35 research outputs found
Effect of combination feeding of Nannochloropsis and freshwater Chlorella on the fatty acid composition of rotifer Brachionus plicatilis in a continuous culture
Effect of hormone injection frequency on the lipid content and fatty acid compositions in gonad, muscle and liver of Anguilla japonica during artificial maturation
Changes in European eel ovary development and body and ovary chemistry during stimulated maturation under controlled conditions: preliminary data
Analysis of the key elements of FFAT-like motifs identifies new proteins that potentially bind VAP on the ER, including two AKAPs and FAPP2.
Two phenylalanines (FF) in an acidic tract (FFAT)-motifs were originally described as having seven elements: an acidic flanking region followed by 6 residues (EFFDA-E). Such motifs are found in several lipid transfer protein (LTP) families, and they interact with a protein on the cytosolic face of the ER called vesicle-associated membrane protein-associated protein (VAP). Mutation of which causes ER stress and motor neuron disease, making it important to determine which proteins bind VAP. Among other proteins that bind VAP, some contain FFAT-like motifs that are missing one or more of the seven elements. Defining how much variation is tolerated in FFAT-like motifs is a preliminary step prior to the identification of the full range of VAP interactors
Life history traits influence in gonad composition of two sympatric species of flatfish
AbstractParalichthys orbignyanus and Paralichthys patagonicus are flatfish with different life history traits, having in common the condition of breeding in seawater. Paralichthys patagonicus remain their whole life in open seawater and Paralichthys orbignyanus are sometimes found in brackish water bodies. As marine and estuarine food webs have different fatty acid (FA) compositions, the aim of this study was to characterize the gonadal maturation of P. orbignyanus and P. patagonicus females through the analysis of lipid content and FA profile in order to understand to what extent life history traits are reflected in the ovarian composition. During gonadal maturation lipid content increased and FA profiles changed in both species, but the lipid increase was greater in P. orbignyanus. The N-3FA and n-3HUFA proportions increased in both species but were higher in P. orbignyanus. The differences between the lifestyles of these species were reflected in the ovarian FA profile mainly as a result of differences in their FA metabolism, causing a greater accumulation of n-3FA and n-3HUFA in P. orbignyanus than in P. patagonicus. The higher lipid accumulation in P. orbignyanus’ ovaries could indicate that this species, feeding in brackish water bodies, has the possibility of storing more energy than P. patagonicus
Pulse programs for sensitivity enhanced 13C-direct detection (H)CACO experiments: seHCACO and siHCACO
Bruker pulse programs for seHCACO and siHCACO experiments
For reference, see:
K. Furuita, T. Sugiki, M. Takamuku, Y. Hattori, M. So, Y. Kawata, T. Ikegami, T. Fujiwara, C. Kojima, Sensitivity enhancement by sequential data acquisition for 13C-direct detection NMR, J. Magn. Reson. 322 (2021) 106878. doi:10.1016/j.jmr.2020.106878