An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface

An oxygen gradient formed along the length of colonic crypts supports stem-cell proliferation at the normoxic crypt base while supporting obligate anaerobe growth in the anoxic colonic lumen. Primary human colonic epithelial cells derived from human gastrointestinal stem cells were cultured within a device possessing materials of tailored oxygen permeability to produce an oxygen-depleted luminal (0.8% ± 0.1% O2) and oxygen-rich basal (11.1% ± 0.5% O2) compartment. This oxygen difference created a stable oxygen gradient across the colonic epithelial cells which remained viable and properly polarized. Facultative and obligate anaerobes Lactobacillus rhamnosus, Bifidobacterium adolescentis, and Clostridium difficile grew readily within the luminal compartment. When formed along the length of an in vitro crypt, the oxygen gradient facilitated cell compartmentalization within the crypt by enhancing confinement of the proliferative cells to the crypt base. This platform provides a simple system to create a physiological oxygen gradient across an intestinal mimic while simultaneously supporting anaerobe co-culture

The Dark Sides of Social Policy: From Neoliberalism to Resurgent Right-wing Populism

This Forum Debate explores the confluence of neoliberal, populist, conservative and reactionary influences on contemporary ideologies and practices of social policy, with a focus on the poorer peripheries of global

Status Report Of The Schenberg Gravitational Wave Antenna

Here we present a status report of the Schenberg antenna. In the past three years it has gone to a radical upgrading operation, in which we have been installing a 1K pot dilution refrigerator, cabling and amplifiers for nine transducer circuits, designing a new suspension and vibration isolation system for the microstrip antennas, and developing a full set of new transducers, microstrip antennas, and oscillators. We are also studying an innovative approach, which could transform Schenberg into a broadband gravitational wave detector.3631Aguiar, O.D., (2002) Class. Quantum Grav., 19, p. 1949Aguiar, O.D., (2004) Class. Quantum Grav., 21, pp. S457Aguiar, O.D., (2005) Class. Quantum Grav., 22, pp. S209Aguiar, O.D., (2006) Class. Quantum Grav., 23, pp. S239Aguiar, O.D., (2008) Class. Quantum Grav., 25, p. 114042Costa, C.A., (2008) Class. Quantum Grav., 25, p. 184002Johnson, W.W., Merkowitz, S.M., (1993) Phys. Rev. Lett., 70, p. 2367Coccia, E., Lobo, J.A., Ortega, J.A., (1995) Phys. Rev. D, 52, p. 3735Thorne, K.S., (1978) Phys. Rev. Lett., 40, p. 667Tobar, M.E., Ivanov, E.N., Blair, D.G., (2000) Gen. Rel. Grav., 32, p. 1799De Waard, (2005) Class. Quantum Grav., 22, pp. S215Vinet, J.-Y., (2010) Research in Astron Astrophys., 10, p. 956Costa, C.A., Aguiar, O.D., Magalhães, N.S., (2004) Class. Quantum Grav., 21, pp. S827Forward, R.L., (1971) Gen. Rel. Grav., 2, p. 149Eardley, D.M., Lee, D.L., Lightman, A.P., Wagoner, R.V., Will, C.M., (1973) Phys. Rev. Lett., 30, p. 884Bianchi, M., Coccia, E., Colacino, C.N., Fafone, V., Fucito, F., (1996) Class. Quantum Grav., 13, p. 2865Andrade, L.A., (2009) Microwave and Optical Tech. Lett., 51, p. 1120Furtado, S.R., (2012), in preparationIvanov, E.N., Hartnett, J.G., Tobar, M.E., (2000) IEEE Trans. Ultrason., Ferroelect., Freq. Contr., 47, p. 1526Pimentel, G.L., (2008) J. Phys. Conf. Series, 122, p. 012028Aguiar, (2009) Int. J. Modern Phys. D, 18, p. 2317Furtado, S.R., (2009), Ph.D. Thesis at INPE, not publishedBraginsky, V.B., Vorontsov, Y.I., Thorne, K.S., (1980) Science, 209, p. 547Thorne, K.S., The Quantum Limit for Gravitational-Wave Detectors and Methods of Circumventing It (1979) Sources of Gravitational Waves, p. 49. , ed. L L Smarr, Cambridge University Press, Cambridge, US

Theotonio dos Santos (1936-2018), the revolutionary intellectual who pioneered dependency theory

This article analyzes the origins and development of the dependency theory through the life and work of Theotonio Dos Santos. His formative years at the academy and his early political activism in Brazil are examined, particularly, his time at the University of Brasilia with Vania Bambirra, Ruy Mauro Marini and André Gunder Frank ("the quartet"). This is followed by a discussion about their years of exile in Chile where the quartet regrouped at the Center for Socio-Economic Studies (ceso) of the University of Chile, and where they wrote their founding texts on dependency theory. Chile provided fertile ground for the development of this theory due to its intellectual climate, institutionality, and the country's political transformations in the late 1960s and early 1970s. The military overthrow of the Allende government on that fateful September 11th, 1973 forced the quartet once again into exile. The article continues with an analysis of Dos Santos' writings during his exile in Mexico and then back in Brazil. During this period he became involved with world system theory which culminated in the publication of his extensive trilogy on the contemporary crisis of capitalism and social theory