The freshwater crayfish Austropotamobius pallipes in South Tyrol. Heritage species and bioindicator

Rapid decline of crayfish in European freshwaters and continuing threat necessitate integrated actions in conservation and management of native crayfish populations. Besides biological reasons (diseases, plague), the impact of toxic and harmful substances (fertilisers, herbicides) or wastewater effluents, habitat alteration or fragmentation have been responsible for their decline in some regions. The same is true for the region of South Tyrol, where compared to previous investigations, only 10 of a former total of 15 crayfish locations in the water bodies could be affirmed. Although two new populations of the non-indigenous Astacus astacus were detected, the native Austropotamobius pallipes continues to decline. While many investigations have focused accurately on causal coherences for the decline of native populations, the properties of crayfish facilitate to reverse the situation. In a few examples, the potential of Austropotamobius pallipes, the native crayfish in South Tyrol, as “surrogate species” for effective biological conservation is discussed. Given the various adequate attributes of freshwater crayfish as surrogate species (including indicator species, umbrella species and flagship species qualities), they may help to advance not only the crayfish situation itself but also freshwater ecosystem properties in general

Tolerance of three European native species of crayfish to hypoxia.

Species that can act as indicators of ecosystem health offer a valuable tool in the management of natural resources. Crayfish have been suggested as bioindicators of water quality in Europe and at least one species (Austropotamobius pallipes) has been studied to determine its tolerance to pollution and its potential as a bioindicator. The genus Austropotamobius includes three crayfish species native to western Europe: A. pallipes, A. italicus and A. torrentium. It was hypothesised that because of their geographical and habitat distribution, the three Austropotamobius species might vary in their value as a bioindicator of water quality. Crayfish of species A. pallipes and A. italicus were subjected to three different treatments: hypoxia (treatment 3, approx 3 mg 1(-1) O-2), light hypoxia (treatment 2, approx 5.5 mg 1(-1) O-2) and normoxia (treatment 1, control, approx 8.5 mg 1(-1) O-2). A. torrentium crayfish were only subjected to treatment 1 (control) and 3. Variations in haemolymph sodium, calcium and chloride were used as a biomarker and concentrations were measured before and after treatment to evaluate hypoxia-induced stress. Significant differences in the concentrations of sodium between the control groups (treatment 1, normoxia) and the experimental groups (treatment 3, 3 mg 1(-1) O-2) were found in the species A. pallipes and A. torrentium. Groups of A. italicus did not show any significant difference between treatments in sodium concentrations but in chloride concentrations. Crayfish of all three species demonstrated a disruption in the ion exchange process in hypoxia, but all tolerated very low oxygen concentration for an extended period of time

Assessment of immunogenicity of romiplostim in clinical studies with ITP subjects

Romiplostim is an Fc-peptide fusion protein that activates intracellular transcriptional pathways via the thrombopoietin (TPO) receptor leading to increased platelet production. Romiplostim has been engineered to have no amino acid sequence homology to endogenous TPO. Recombinant protein therapeutics can be at a risk of development of an antibody response that can impact efficacy and safety. Hence, a strategy to detect potential antibody formation to the drug and to related endogenous molecules can be useful. The immunogenicity assessment strategy involved both the detection and characterization of binding and neutralizing antibodies. The method for detection was based on a surface plasmon resonance biosensor platform using the Biacore 3000. Samples that tested positive for binding antibodies in the Biacore immunoassay were then tested in a neutralization assay. Serum samples from 225 subjects with immune thrombocytopenic purpura (ITP) dosed with romiplostim and 45 ITP subjects dosed with placebo were tested for romiplostim and TPO antibodies. Prior to romiplostim treatment, 17 subjects (7%) tested romiplostim antibody positive and 12 subjects (5%) tested TPO antibody positive for pre-existing binding antibodies. After romiplostim exposure, 11% of the subjects exhibited binding antibodies against romiplostim and 5% of the subjects with ITP showed binding antibodies against TPO. The antibodies against romiplostim did not cross-react with TPO and vice versa. No cases of anti-TPO neutralizing antibodies were detected in romiplostim-treated subjects. The incidence of anti-romiplostim neutralizing antibodies to romiplostim was 0.4% (one subject); this subject tested negative at the time of follow-up 4 months later. No impact on platelet profiles were apparent in subjects that had antibodies to romiplostim to date. In summary, administration of romiplostim in ITP subjects resulted in the development of a binding antibody response against romiplostim and TPO ligand. One subject developed a neutralizing antibody response to romiplostim that impacted the platelet counts of this subject. No neutralizing antibodies to endogenous TPO were observed

Multiple drivers of decline in the global status of freshwater crayfish (Decapoda: Astacidea)

International audienceRates of biodiversity loss are higher in freshwater ecosystems than in most terrestrial or marine ecosystems, making freshwater conservation a priority. However, prioritization methods are impeded by insufficient knowledge on the distribution and conservation status of freshwater taxa, particularly invertebrates. We evaluated the extinction risk of the world's 590 freshwater crayfish species using the IUCN Categories and Criteria and found 32% of all species are threatened with extinction. The level of extinction risk differed between families, with proportionally more threatened species in the Parastacidae and Astacidae than in the Cambaridae. Four described species were Extinct and 21% were assessed as Data Deficient. There was geographical variation in the dominant threats affecting the main centres of crayfish diversity. The majority of threatened US and Mexican species face threats associated with urban development, pollution, damming and water management. Conversely, the majority of Australian threatened species are affected by climate change, harvesting, agriculture and invasive species. Only a small proportion of crayfish are found within the boundaries of protected areas, suggesting that alternative means of long-term protection will be required. Our study highlights many of the significant challenges yet to come for freshwater biodiversity unless conservation planning shifts from a reactive to proactive approach

Environmental harshness mediates the quality of periphyton and chironomid body mass in alpine streams

Glacier retreat alters physical and chemical characteristics and biological communities of glacier-fed streams. Functional relationships, including effects of different food-source qualities on the density and biomass of primary consumers, are insufficiently known in alpine and subalpine headwaters. We measured: 1) the relative abundance of diatoms 1 chrysophytes, green algae, and cyanobacteria in the periphyton and assemblage patterns of the 2 dominant chironomid subfamilies, Diamesinae and Orthocladiinae, and 2) differences in chironomid body mass as an unexpected response to living conditions in harsh alpine stream ecosystems. Diatoms 1 chrysophytes dominated in harsh environments, and cyanobacteria were abundant in more benign streams. Patterns in algal dominance corresponded to differences in individual body mass of Diamesinae and Orthocladiinae among streams. Body mass of glacial-river specialists Diamesa steinboecki and Diamesa latitarsis gr. was higher in harsh than in benign streams, but other Diamesinae and Orthocladiinae biomass did not differ between harsh or less harsh conditions. Plausible explanations include higher nutritional quality of algal classes in periphyton and less competition for specialists in harsh than in benign streams. Our results indicate that harsh environmental conditions, such as low temperatures and high turbidity, lead to lower taxon numbers and food quality and that food quality influences invertebrate occurrence and body mass in alpine and subalpine headwater streams. Closely related species respond differently to specific environmental conditions and may perform differing functions. This result widens our general understanding of climate-change effects on alpine stream ecosystems and provides additional explanations for species abilities and performance

Benthic algae in high altitude streams of the Alps - A neglected component of the aquatic biota

This is a review on benthic algae from streams situated above the tree line in the Alps. It integrates published and unpublished data from alpine streams in Austria and in the Trento Province (Northern Italy). The main focus is on the structural and taxonomic composition of benthic algae including macro- and micro-algae and their contribution to the epilithic biofilm and the stream food-web. The environmental conditions relevant to algae within the two major stream types, the glacial (glacier-fed) and non-glacial krenal (spring-fed) stream are discussed. The paper considers both the maximum possible structural complexity of transverse algae zonation in cascading alpine/subalpine stream segments, and the effects of glacial water on species richness in the Central Alps in Austria. Autecological data are given for 46 common diatoms from 42 sites in the Central Eastern and Southern Central Alps and for 30 algae in addition to diatoms for 22 streams in the Central Eastern Alps. Since data on responses of benthic algae to the harsh conditions in high altitude Alpine streams are very scarce, results from literature and the author's experiences from these and other mountain stream types are synthesized to formulate major objectives for future research in benthic high altitude Alpine stream ecology. © Springer 2006

Crenic habitats, hotspots for freshwater biodiversity conservation: Toward an understanding of their ecology

Springs are unique aquatic habitats that contribute significantly to local and regional biodiversity because of their high habitat complexity and the large number of different spring types. Many springs are small, but they are numerous and often of high water quality, and thus, provide habitats for species that are rare elsewhere because of their sensitivity to anthropogenic impacts (least-impaired habitat relicts). Springs are often species-rich and contain a larger number of Red List taxa than other aquatic habitats. Hydrological factors, particularly flow permanence, water chemistry, and temperature are important ecological factors determining species distribution and community composition. Despite their importance for biodiversity and water quality, springs are much less studied than other aquatic ecosystems. They also are insufficiently covered by protective legislation, often resulting in the destruction of their natural habitat. The authors of papers in this special issue describe specific spring biota, including multitaxon studies, and discuss the role of environmental factors, habitat variability at different spatial and temporal scales, and the importance of natural and anthropogenic disturbance in spring habitats. They suggest directions for future research, including defining reference conditions for springs and their role in long-term ecological research, the development of quality-assessment methods, and their more sustainable use as freshwater resources. © 2012 The Society for Freshwater Science