Differential effects of caffeine on hair shaft elongation, matrix and outer root sheath keratinocyte proliferation, and TGF-beta2-/IGF-1-mediated regulation of hair cycle in male and female human hair follicles in vitro.

BACKGROUND: Caffeine reportedly counteracts the suppression of hair shaft production by testosterone in organ-cultured male human hair follicles (HFs). OBJECTIVES: We aimed at investigating the impact of caffeine a) on additional key hair growth parameters, b) on major hair growth-regulatory growth factors and c) on male versus female HFs in the presence of testosterone. METHODS: Microdissected male and female human scalp HFs were treated in serum-free organ culture for 120 h with testosterone alone (0,5 mug/ml) or in combination with caffeine (0.005-0.0005%), and effects on hair shaft elongation, HF cycling (i.e. anagen-catagen transition), hair matrix keratinocyte proliferation and expression of a key catagen inducer, transforming growth factor beta2 (TGF-beta2), and anagen-prolonging insulin-like growth factor 1 (IGF-1) were evaluated by quantitative (immuno-) histomorphometry. Caffeine effects were further investigated in human outer root sheath keratinocytes (ORSK). RESULTS: Caffeine enhanced hair shaft elongation, prolonged anagen duration and stimulated hair matrix keratinocyte proliferation. Female HFs showed higher sensitivity to caffeine compared to male HFs. Caffeine counteracted testosterone-enhanced TGF-beta2 protein expression in male HFs. In female HFs, testosterone failed to induce TGF-beta2 expression, while caffeine reduced it. In male and female HFs, caffeine enhanced IGF-1 protein expression. In ORSK, caffeine stimulated cell proliferation, inhibited apoptosis/necrosis, up-regulated IGF-1 gene expression and protein secretion, while TGF-beta2 protein secretion was down-regulated. CONCLUSIONS: This study reveals new growth-promoting effects of caffeine on human hair follicles of both genders at different (molecular, cellular and organ) levels. This article is protected by copyright. All rights reserved

Joint H.E.S.S. and Fermi-LAT analysis of the region around PSR J1813-1749

HESS J1813-178 is one of the brightest sources detected during the first HESS Galactic Plane survey. The compact source, also detected by MAGIC, is believed to be a pulsar wind nebula powered by one of the most powerful pulsars known in the Galaxy, PSR J1813-1749 with a spin-down luminosity of $\dot{\mathrm{E}} = 5.6 \cdot 10^{37}\,\mathrm{erg}\,\mathrm{s}^{-1}$. With its extreme physical properties, as well as the pulsar's young age of 5.6 kyrs, the $\gamma$-rays detected in this region allow us to study the evolution of a highly atypical system. Previous studies of the region in the GeV energy range show emission extended beyond the size of the compact H.E.S.S. source. Using the archival H.E.S.S. data with improved background methods, we perform a detailed morphological and spectral analysis of the region. Additionally to the compact, bright emission component, we find significantly extended emission, whose position is coincident with HESS J1813-178. We reanalyse the region in GeV and derive a joint-model in order to find a continuous description of the emission in the region from GeV to TeV. Using the results derived in this analysis, as well as X-ray and radio data of the region, we perform multi-wavelength spectral modeling. Possible hadronic or leptonic origins of the $\gamma$-ray emission are investigated, and the diffusion parameters necessary to explain the extended emission are examined.Comment: 8 pages, 4 figures, 1 table, In proceedings of ICRC202

Relationship Between Intraseasonal Oscillation and Subtropical Wind Maxima Over the South Pacific Ocean

The significance of tropical heat sources on higher latitude jet streams has been examined by numerous investigators. Hurrell and Vincent (1990) provide a summary of many of these investigations in their observational case study of the relationship between tropical heating and subtropical wind maxima in the Southern Hemisphere during SOP-1, FGGE. They showed that the divergent outflow from tropical heating associated with the South Pacific Convergence Zone (SPCZ), acted on by the coriolis force, was an important factor in maintaining the subtropical jet on the poleward side of the SPCZ during the period, 6-20 January 1979. They found a similar, but weaker relationship, over the southern Indian Ocean from 3-17 February 1979, a period when the SPCZ heating was greatly reduced and the jet was essentially non-existent. Since their findings were based on a case study and involved the use of the highly-specialized FGGE data set, the natural questions which arose were: (1) Is this relationship a regular feature of the circulation over the South Pacific? and, (2) If so, can it be detected with a routine data set? Another question posed by Hurrell and Vincent in their papers was:(3) How important was the intraseasonal oscillation in causing the enhanced heating and divergent outflow in the Pacific Ocean in January and southern Indian Ocean in February? The purpose of the present paper is to address the answer to these three questions. To accomplish this, some circulation features for an entire warm season in the Southern Hemisphere were examined. The year selected was 1984-85, and the warm season consisted of the 6-month period, 1 November 1984 - 30 April 1985. This period was chosen because there were numerous cases of the westerly wind maxima over the South Pacific and the intraseasonal oscillation was well documented

Nine challenges in incorporating the dynamics of behaviour in infectious diseases models.

Traditionally, the spread of infectious diseases in human populations has been modelled with static parameters. These parameters, however, can change when individuals change their behaviour. If these changes are themselves influenced by the disease dynamics, there is scope for mechanistic models of behaviour to improve our understanding of this interaction. Here, we present challenges in modelling changes in behaviour relating to disease dynamics, specifically: how to incorporate behavioural changes in models of infectious disease dynamics, how to inform measurement of relevant behaviour to parameterise such models, and how to determine the impact of behavioural changes on observed disease dynamics

Variation in Isoprene Emission from Quercus rubra: Sources, Causes, and Consequences for Estimating Fluxes

Isoprene is the dominant volatile organic compound produced in many forest systems. Uncertainty in estimates of leaf level isoprene emission rate stems from an insufficient understanding of the patterns and processes controlling isoprene emission capacity in plant leaves. Previous studies suggest that variation in isoprene emission capacity is substantial; however, it is not known at what scale emission capacity is the most variable. Identifying the sources of variation in emission capacity has implications for conducting measurements and for model development, which will ultimately improve emission estimates and models of tropospheric chemistry. In addition, understanding the sources of variation will help to develop a comprehensive understanding of the physiological controls over isoprene emission. This study applied a variance partitioning approach to identify the major sources of variation in isoprene emission capacity from two populations of northern red oak (Quercus rubra) over three growing seasons. Specifically, we evaluated variation due to climate, populations, trees, branches, leaves, seasons, and years. Overall, the dominant source of variation was the effect of a moderate drought event. In the years without drought events, variation among individual trees (intraspecific) explained approximately 60% of the total variance. Within the midseason, isoprene emission capacity of sun leaves varied by a factor of 2 among trees. During the third year a moderate 20-day drought event caused isoprene emission capacity to decrease fourfold, and the relative importance of intraspecific variation was reduced to 24% of total variance. Overall, ambient temperature, light, and a drought index were poor predictors of isoprene emission capacity over a 0 to 14-day period across growing seasons. The drought event captured in this study emphasizes the need to incorporate environmental influences into leaf level emission models

(Cyclopentadienone)iron-Catalyzed Transfer Dehydrogenation of Symmetrical and Unsymmetrical Diols to Lactones

Air-stable iron carbonyl compounds bearing cyclopentadienone ligands with varying substitution were explored as catalysts in dehydrogenative diol lactonization reactions using acetone as both the solvent and hydrogen acceptor. Two catalysts with trimethylsilyl groups in the 2- and 5-positions, [2,5-(SiMe3)2-3,4-(CH2)4(η4-C4C═O)]Fe(CO)3 (1) and [2,5-(SiMe3)2-3,4-(CH2)3(η4-C4C═O)]Fe(CO)3 (2), were found to be the most active, with 2 being the most selective in the lactonization of diols containing both primary and secondary alcohols. Lactones containing five-, six-, and seven-membered rings were successfully synthesized, and no over-oxidations to carboxylic acids were detected. The lactonization of unsymmetrical diols containing two primary alcohols occurred with catalyst 1, but selectivity was low based on alcohol electronics and modest based on alcohol sterics. Evidence for a transfer dehydrogenation mechanism was found, and insight into the origin of selectivity in the lactonization of 1°/2° diols was obtained. Additionally, spectroscopic evidence for a trimethylamine-ligated iron species formed in solution during the reaction was discovered

Computational Modeling Using OpenSim to Simulate a Squat Exercise Motion

Long duration space travel to destinations such as Mars or an asteroid will expose astronauts to extended periods of reduced gravity. Astronauts will use an exercise regime for the duration of the space flight to minimize the loss of bone density, muscle mass and aerobic capacity that occurs during exposure to a reduced gravity environment. Since the area available in the spacecraft for an exercise device is limited and gravity is not present to aid loading, compact resistance exercise device prototypes are being developed. Since it is difficult to rigorously test these proposed devices in space flight, computational modeling provides an estimation of the muscle forces, joint torques and joint loads during exercise to gain insight on the efficacy to protect the musculoskeletal health of astronauts

Controlled order rearrangement encryption for quantum key distribution

A novel technique is devised to perform orthogonal state quantum key distribution. In this scheme, entangled parts of a quantum information carrier are sent from Alice to Bob through two quantum channels. However before the transmission, the orders of the quantum information carrier in one channel is reordered so that Eve can not steal useful information. At the receiver's end, the order of the quantum information carrier is restored. The order rearrangement operation in both parties is controlled by a prior shared control key which is used repeatedly in a quantum key distribution session.Comment: 5 pages and 2 figure