Expression of the insulin-like growth factor-II/mannose-6-phosphate receptor in multiple human tissues during fetal life and early infancy

The insulin like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptor has been detected in many cells and tissues. In the rat, there is a dramatic developmental regulation of IGF-II/M6P receptor expression, the receptor being high in fetal and neonatal tissues and declining thereafter. We have systematically studied the expression of the human IGF-II/M6P receptor protein in tissues from 10 human fetuses and infants (age 23 weeks gestation to 24 months postnatal). We have asked 1) whether there is differential expression among different organs, and 2) whether or not the human IGF-II/M6P receptor is developmentally regulated from 23 weeks gestation to 24 months postnatal. Protein was extracted from human tissues using a buffer containing 2% sodium dodecyl sulfate and 2% Triton X-100. Aliquots of the protein extracts were analyzed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and immunoblotting using an anti-IGF- II/M6P receptor antiserum (no. 66416) and 125I-protein A or an immunoperoxidase stain. IGF-II/M6P receptor immunoreactivity was detected in all tissues studied with the highest amount of receptor being expressed in heart, thymus, and kidney and the lowest receptor content being measured in brain and muscle. The receptor content in ovary, testis, lung, and spleen was intermediate. The apparent molecular weight of the IGF-II/M6P receptor (220,000 kilos without reduction of disulfide bonds) varied among the different tissues: in brain the receptor was of lower molecular weight than in other organs. Immunoquantitation experiments employing 125I-protein A and protein extracts from human kidney at different ages revealed a small, albeit not significant, difference of the receptor content between fetal and postnatal tissues: as in other species, larger amounts of receptor seemed to be present in fetal than in postnatal organs. In addition, no significant difference of the receptor content between human fetal liver and early postnatal liver was measured employing 125I-protein A- immunoquantitation in three fetal and five postnatal liver tissue samples. The distribution of IGF-binding protein (IGEBP) species, another abundant and major class of IGF binding principles, was also measured in human fetal and early postnatal lung, liver, kidney, muscle, and brain using Western ligand blotting with 125I-IGF-II: as with IGF-II/M6P receptor immunoreactivity there was differential expression of the different classes of IGFBPs in the various organs

Examining the structural composition and longitudinal change of team identification.

The propensity of strongly identified fans to contribute positive organizational outcomes for sport teams underpins why team identification maintains a central position in sport management. In the present study we examine the multidimensional structure, stability, and interrelationships between the dimensions of team identification, using longitudinal data (April 2011-April 2012) collected from fans of a new Australian Rules football team (N=602). A Confirmatory Factor Analysis (CFA) of the team identification items included (measured using the Team*ID scale), supported a five-dimensional model structure. This model was subsequently computed as a longitudinal CFA to test the configural and metric invariance of the Team*ID scale. We used a cross-lagged panel model to examine the longitudinal stability of, and interrelationships between,the dimensions: affect, behavioral involvement, cognitive awareness, private evaluation, and public evaluation. Each dimension displayed relative stability over time. In addition, public evaluation and private evaluation in April 2011 displayed a positive relationship with behavioral involvement in April 2012. Similarly, cognitive awareness in April 2011 predicted increases in public evaluation in April 2012 two. We conclude with implications for theory and practice

TARGET: A Digitizing And Trigger ASIC For The Cherenkov Telescope Array

The future ground-based gamma-ray observatory Cherenkov Telescope Array (CTA) will feature multiple types of imaging atmospheric Cherenkov telescopes, each with thousands of pixels. To be affordable, camera concepts for these telescopes have to feature low cost per channel and at the same time meet the requirements for CTA in order to achieve the desired scientific goals. We present the concept of the TeV Array Readout Electronics with GSa/s sampling and Event Trigger (TARGET) Application Specific Circuit (ASIC), envisaged to be used in the cameras of various CTA telescopes, e.g. the Gamma-ray Cherenkov Telescope (GCT), a proposed 2-Mirror Small-Sized Telescope, and the Schwarzschild-Couder Telescope (SCT), a proposed Medium-Sized Telescope. In the latest version of this readout concept the sampling and trigger parts are split into dedicated ASICs, TARGET C and T5TEA, both providing 16 parallel input channels. TARGET C features a tunable sampling rate (usually 1 GSa/s), a 16k sample deep buffer for each channel and on-demand digitization and transmission of waveforms with typical spans of ~100 ns. The trigger ASIC, T5TEA, provides 4 low voltage differential signal (LVDS) trigger outputs and can generate a pedestal voltage independently for each channel. Trigger signals are generated by T5TEA based on the analog sum of the input in four independent groups of four adjacent channels and compared to a threshold set by the user. Thus, T5TEA generates four LVDS trigger outputs, as well as 16 pedestal voltages fed to TARGET C independently for each channel. We show preliminary results of the characterization and testing of TARGET C and T5TEA.Comment: 6 pages, 8 figures, Proceedings of the 6th International Symposium on High-Energy Gamma-Ray Astronomy (Gamma2016

A multiple scale model for tumor growth

We present a physiologically structured lattice model for vascular tumor growth which accounts for blood flow and structural adaptation of the vasculature, transport of oxygen, interaction between cancerous and normal tissue, cell division, apoptosis, vascular endothelial growth factor release, and the coupling between these processes. Simulations of the model are used to investigate the effects of nutrient heterogeneity, growth and invasion of cancerous tissue, and emergent growth laws

Fermi-LAT and Suzaku Observations of the Radio Galaxy Centaurus B

Centaurus B is a nearby radio galaxy positioned in the Southern hemisphere close to the Galactic plane. Here we present a detailed analysis of about 43 months of accumulated Fermi-LAT data of the gamma-ray counterpart of the source initially reported in the 2nd Fermi-LAT catalog, and of newly acquired Suzaku X-ray data. We confirm its detection at GeV photon energies, and analyze the extension and variability of the gamma-ray source in the LAT dataset, in which it appears as a steady gamma-ray emitter. The X-ray core of Centaurus B is detected as a bright source of a continuum radiation. We do not detect however any diffuse X-ray emission from the known radio lobes, with the provided upper limit only marginally consistent with the previously claimed ASCA flux. Two scenarios that connect the X-ray and gamma-ray properties are considered. In the first one, we assume that the diffuse non-thermal X-ray emission component is not significantly below the derived Suzaku upper limit. In this case, modeling the inverse-Compton emission shows that the observed gamma-ray flux of the source may in principle be produced within the lobes. This association would imply that efficient in-situ acceleration of the radiating electrons is occurring and that the lobes are dominated by the pressure from the relativistic particles. In the second scenario, with the diffuse X-ray emission well below the Suzaku upper limits, the lobes in the system are instead dominated by the magnetic pressure. In this case, the observed gamma-ray flux is not likely to be produced within the lobes, but instead within the nuclear parts of the jet. By means of synchrotron self-Compton modeling we show that this possibility could be consistent with the broad-band data collected for the unresolved core of Centaurus B, including the newly derived Suzaku spectrum.Comment: Accepted for publication in A&A. 11 page

A search for VHE counterparts of Galactic Fermi bright sources and MeV to TeV spectral characterization

Very high-energy (VHE; E>100 GeV) gamma-rays have been detected from a wide range of astronomical objects, such as pulsar wind nebulae (PWNe), supernova remnants (SNRs), giant molecular clouds, gamma-ray binaries, the Galactic Center, active galactic nuclei (AGN), radio galaxies, starburst galaxies, and possibly star-forming regions as well. At lower energies, observations using the Large Area Telescope (LAT) onboard Fermi provide a rich set of data which can be used to study the behavior of cosmic accelerators in the MeV to TeV energy bands. In particular, the improved angular resolution of current telescopes in both bands compared to previous instruments significantly reduces source confusion and facilitates the identification of associated counterparts at lower energies. In this paper, a comprehensive search for VHE gamma-ray sources which are spatially coincident with Galactic Fermi/LAT bright sources is performed, and the available MeV to TeV spectra of coincident sources are compared. It is found that bright LAT GeV sources are correlated with TeV sources, in contrast to previous studies using EGRET data. Moreover, a single spectral component seems unable to describe the MeV to TeV spectra of many coincident GeV/TeV sources. It has been suggested that gamma-ray pulsars may be accompanied by VHE gamma-ray emitting nebulae, a hypothesis that can be tested with VHE observations of these pulsars.Comment: Astronomy and Astrophysics, in press, 17 pages, 12 figures, 5 table