Therapeutic effects of Reb eyedrop administration on tear secretion in the murine model of SS.

<p>Average tear volumes after pilocarpine administration (2.5 mg/kg) were measured for 5 min (0–5 min and 5–10 min). Results are expressed as means ± SD for 5 mice per group. Control, nonthymectomized (non-Tx) NFS/<i>sld</i> mice. ** <i>P</i><0.005<b>.</b></p

Lactoferrin in tears from Reb-treated mice and direct effect of Reb on lacrimal glands.

<p>(A) The lactoferrin concentrations in the tears from Reb-treated mice were determined using ELISA. Results are expressed as mean ± SD for five mice per group. (B) Lacrimal gland cells from Tx-NFS/<i>sld</i> mice were cultured with or without Reb (0.25 mM) for 6 h, and the lactoferrin mRNA levels in cultured Lg fragments were then analyzed using real-time RT-PCR. Results are expressed as mean ± SD of triplicate determinations per group from 3 independent experiments. *<i>P</i><0.05, **<i>P</i><0.01.</p

Lactoferrin in tears from Reb-treated mice and direct effect of Reb on lacrimal glands.

<p>(A) The lactoferrin concentrations in the tears from Reb-treated mice were determined using ELISA. Results are expressed as mean ± SD for five mice per group. (B) Lacrimal gland cells from Tx-NFS/<i>sld</i> mice were cultured with or without Reb (0.25 mM) for 6 h, and the lactoferrin mRNA levels in cultured Lg fragments were then analyzed using real-time RT-PCR. Results are expressed as mean ± SD of triplicate determinations per group from 3 independent experiments. *<i>P</i><0.05, **<i>P</i><0.01.</p

Anti-inflammatory proteins in the ocular lesions in Reb-treated murine model of SS.

<p>The mRNA expression levels of TGF-β (A), IL-10 (B), and mucin 5AC (C) in conjunctival tissues in Reb-treated (0% and 1%) mice were analyzed using real-time RT-PCR. Results expressed as mean ± SD for five mice per group.</p

Effect of Reb eyedrops on the ocular lesions in the murine model of SS.

<p>(A) Ocular surface lesions in Reb-treated mice were examined using fluorescein staining of the ocular surface. Representative photomicrographs for each group are shown. (B) The scores for corneal epithelial disorders. Results are expressed as mean ± SD for five mice per group. *<i>P</i><0.05, **<i>P</i><0.005.</p