Deer reduce habitat quality for a woodland songbird: evidence from settlement patterns, demographic parameters, and body condition.

Understanding avian responses to ungulate-induced habitat modification is important because deer populations are increasing across much of temperate Europe and North America. Our experimental study examined whether habitat quality for Blackcaps (Sylvia atricapilla) in young woodland in eastern England was affected by deer, by comparing Blackcap behavior, abundance, and condition between paired plots (half of each pair protected from deer). The vegetation in each pair of plots was the same age. The Blackcap is an ideal model species for testing effects of deer on avian habitat quality because it is dependent on dense understory vegetation and is abundant throughout much of Europe. We compared timing of settlement, abundance, age structure (second-year vs. after-second-year), and phenotypic quality (measured as a body condition index, body mass divided by tarsus length) between experimental and control plots. We used point counts to examine Blackcap distribution, and standardized mist netting to collect demographic and biometric data. Incidence of singing Blackcaps was higher in nonbrowsed than in browsed plots, and singing males were recorded in nonbrowsed plots earlier in the season, indicating earlier and preferential territory establishment. Most Blackcaps, both males and females, were captured in vegetation prior to canopy closure (2–4 years of regrowth). Body condition was superior for male Blackcaps captured in nonbrowsed plots; for second-year males this was most marked in vegetation prior to canopy closure. We conclude that deer browsing in young woodland can alter habitat quality for understory-dependent species, with potential consequences for individual fitness and population productivity beyond the more obvious effects on population density

Single Neuron Analysis by Capillary Electrophoresis with Fluorescence Spectroscopy

AbstractA technique to identify and quantitate simultaneously more than 30 compounds in individual neurons is described. The method uses nanoliter volume sampling, capillary electrophoresis separation, and wavelength-resolved native fluorescence detection. Limits of detection (LODs) range from the low attomole to the femtomole range, with 5-hydroxytryptamine (or serotonin [5-HT]) LODs being ∼20 attomoles. Although the cellular sample matrix is chemically complex, the combination of electrophoretic migration time and fluorescence spectral information allows positive identification of aromatic monoamines, aromatic amino acids and peptides containing them, flavins, adenosine- and guanosine-nucleotide analogs, and other fluorescent compounds. Individual identified neurons from Aplysia californica and Pleurobranchaea californica are used to demonstrate the applicability and figures of merit of this technique

Simulated Cytoskeletal Collapse via Tau Degradation

We present a coarse-grained two dimensional mechanical model for the microtubule-tau bundles in neuronal axons in which we remove taus, as can happen in various neurodegenerative conditions such as Alzheimer's disease, tauopathies, and chronic traumatic encephalopathy. Our simplified model includes (i) taus modeled as entropic springs between microtubules, (ii) removal of taus from the bundles due to phosphorylation, and (iii) a possible depletion force between microtubules due to these dissociated phosphorylated taus. We equilibrate upon tau removal using steepest descent relaxation. In the absence of the depletion force, the transverse rigidity to radial compression of the bundle falls to zero at about 60% tau occupancy, in agreement with standard percolation theory results. However, with the attractive depletion force, spring removal leads to a first order collapse of the bundles over a wide range of tau occupancies for physiologically realizable conditions. While our simplest calculations assume a constant concentration of microtubule intercalants to mediate the depletion force, including a dependence that is linear in the detached taus yields the same collapse. Applying percolation theory to removal of taus at microtubule tips, which are likely to be the protective sites against dynamic instability, we argue that the microtubule instability can only obtain at low tau occupancy, from 0.06-0.30 depending upon the tau coordination at the microtubule tips. Hence, the collapse we discover is likely to be more robust over a wide range of tau occupancies than the dynamic instability. We suggest in vitro tests of our predicted collapse.Comment: 11 pages, 9 figure

Tracking the rapid loss of tidal wetlands in the Yellow Sea

In the Yellow Sea region of East Asia, tidal wetlands are the frontline ecosystem protecting a coastal population of more than 60 million people from storms and sea-level rise. However, unprecedented coastal development has led to growing concern about the status of these ecosystems. We developed a remote-sensing method to assess change over ∼4000 km of the Yellow Sea coastline and discovered extensive losses of the region's principal coastal ecosystem - tidal flats - associated with urban, industrial, and agricultural land reclamations. Our analysis revealed that 28% of tidal flats existing in the 1980s had disappeared by the late 2000s (1.2% annually). Moreover, reference to historical maps suggests that up to 65% of tidal flats were lost over the past five decades. With the region forecast to be a global hotspot of urban expansion, development of the Yellow Sea coastline should pursue a course that minimizes the loss of remaining coastal ecosystems

The Dairy Heard Buyout Program: How Much to Bid. A Computer decision aid approach.

This archival publication may not reflect current scientific knowledge or recommendations. Current information available from the University of Minnesota Extension: https://www.extension.umn.edu

Some comments on graduate training for engineers

QUESTIONING the value and adequacy of postgraduate engineering courses as commonly conducted, L. W. W. Morrow in an article in Electrical Engineering for March 1939, pages 118–22, called for a program of investigation of postgraduate training. Solicited comments of some representative industrialists and educators are presented here

Enzyme based soil stabilization for unpaved road construction

Enzymes as soil stabilizers have been successfully used in road construction in several countries for the past 30 years. However, research has shown that the successful application of these enzymes is case specific, emphasizing that enzyme performance is dependent on subgrade soil type, condition and the type of enzyme used as the stabilizer. A universal standard or a tool for road engineers to assess the performance of stabilized unbound pavements using well-established enzymes is not available to date. The research aims to produce a validated assessment tool which can be used to predict strength enhancement within a generalized statistical framework. The objective of the present study is to identify new materials for developing the assessment tool which supports enzyme based stabilization, as well as to identify the correct construction sequence for such new materials. A series of characterization tests were conducted on several soil types obtained from proposed construction sites. Having identified the suitable soil type to mix with the enzyme, a trial road construction has been performed to investigate the efficiency of the enzyme stabilization along with the correct construction sequence. The enzyme stabilization has showed significant improvement of the road performance as was evidenced from the test results which were based on site soil obtained before and after stabilization. The research will substantially benefit the road construction industry by not only replacing traditional construction methods with economical/reliable approaches, but also eliminating site specific tests required in current practice of enzyme based road construction

The Dairy Heard Buyout Program: How Much to Bid: FINLRB (Financial Long Range budgeting) Approach

This archival publication may not reflect current scientific knowledge or recommendations. Current information available from the University of Minnesota Extension: https://www.extension.umn.edu

Heterogeneity among Isolates Reveals that Fitness in Low Oxygen Correlates with Aspergillus fumigatus Virulence

Previous work has shown that environmental and clinical isolates of Aspergillus fumigatus represent a diverse population that occupies a variety of niches, has extensive genetic diversity, and exhibits virulence heterogeneity in a number of animal models of invasive pulmonary aspergillosis (IPA). However, mechanisms explaining differences in virulence among A. fumigatus isolates remain enigmatic. Here, we report a significant difference in virulence of two common lab strains, CEA10 and AF293, in the murine triamcinolone immunosuppression model of IPA, in which we previously identified severe low oxygen microenvironments surrounding fungal lesions. Therefore, we hypothesize that the ability to thrive within these lesions of low oxygen promotes virulence of A. fumigatus in this model. To test this hypothesis, we performed in vitro fitness and in vivo virulence analyses in the triamcinolone murine model of IPA with 14 environmental and clinical isolates of A. fumigatus Among these isolates, we observed a strong correlation between fitness in low oxygen in vitro and virulence. In further support of our hypothesis, experimental evolution of AF293, a strain that exhibits reduced fitness in low oxygen and reduced virulence in the triamcinolone model of IPA, results in a strain (EVOL20) that has increased hypoxia fitness and a corresponding increase in virulence. Thus, the ability to thrive in low oxygen correlates with virulence of A. fumigatus isolates in the context of steroid-mediated murin