Characteristics of the fads2 gene promoter in marine teleost Epinephelus coioides and role of Sp1-binding site in determining promoter activity

Δ6 fatty acyl desaturase (Fads2) is a rate-limiting enzyme in long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis. Comparative analysis of gene promoters of Fads2 between salmonids and carnivorous marine sh suggested that the lack of binding site for stimulatory protein 1 (Sp1) was responsible for the low expression of fads2 gene of carnivorous marine species. To con rm this speculation, the fads2 candidate promoter (2646 bp) was cloned from carnivorous marine teleost Epinephelus coioides, and 330 bp core regulatory region was identi ed. Several binding sites for transcriptional factors such as nuclear factor 1, nuclear factorY, sterol regulatory element and hepatocyte nuclear factor 4γ were identi ed, while that for Sp1 was shown to be absent in the promoter by both bioinformatic analysis and site-directed mutation. Moreover, after the Sp1-binding site from the fads2 promoter of herbivorous Siganus canaliculatus, the rst marine teleost demonstrated to have LC-PUFA biosynthetic ability, was inserted into the corresponding region of E. coioides fads2 promoter, activity was signi cantly increased. The results provided direct data for the importance of the Sp1- binding site in determining fads2 promoter activity, and indicated that its lack may be a reason for low expression of fads2 and poor LC-PUFA biosynthetic ability in E. coioides

Exploring risk transfer of human brucellosis in the context of livestock agriculture transition: A case study in Shaanxi, China

With the booming of worldwide agriculture intensification, brucellosis, one of the most neglected zoonotic diseases, has become an increasing challenge for global public health. Although the transmission patterns of human brucellosis (HB) have been studied in many regions, the dynamic transfer processes of risk and its driving factors remain poorly understood, especially in the context of agricultural intensification. This study attempted to explore the risk transfer of HB between the exact epidemic areas and the neighboring or distant low-risk areas to explain the impact of livestock agriculture intensification and foodborne infections on the transmission of HB in Shaanxi Province as a case study. We adopted multiple approaches, including test-based methods, model-based methods, and a geographical detector to detect the spatial-temporal dynamic changes of high-risk epidemic areas of HB at the county scale. We also quantitatively estimated how the related factors drove the risk transfer of the disease. Results confirmed the risk transfer pattern of HB with an expansion from north to south in Shaanxi Province and identified two primary transfer routes. In particular, in the traditional epidemic areas of the Shaanbei plateau, the farm agglomeration effect can significantly increase the risk of HB. Meanwhile, retail outlets for milk and dairy products were partially responsible for the foodborne infections of HB in the emerging epidemic areas of Xi'an. This study not only contributed helpful insights to support HB control and prevention in the rapid transition of livestock agriculture but also provided possible directions for further research on foodborne HB infections in urbanized areas

A high-precision correction method in non-rigid 3D motion poses reconstruction

Occlusion, rotation and other factors affect human motion structure because of the incomplete acquired image sequence, resulting in poor performance of non-rigid three-dimensional (3D) motion pose reconstruction. A non-rigid 3D reconstruction and high-precision correction method for motion pose are studied in this paper. A non-rigid imaging model is designed to obtain 3D moving images. According to the frame difference and morphological processing, the background of image is separated and denoised. Combined with motion analysis, 3D motion pose features are extracted as identification of non-rigid 3D motion error actions in a hybrid Convolution Neural Network-Hidden Markov Model to train the correction coefficients, which are used to adjust the pose in 3D motion reconstruction and realise correction. Experimental results show that this method has high precision reconstruction and correction of non-rigid 3D motion pose

資生堂の中国における競争戦略

　Shiseido, Ltd is a major Japanese cosmetics producer. In this paper, we focus on Shiseido’s efforts seizing of business opportunities from China’s reform and opening and spreading the culture of cosmetics from Japan among Chinese women, which have been successful in expanding their business.　We consider competitive strategy of Shiseido in China’s cosmetics industry using SWOT analysis and Porter’s five forces. We also discuss management strategies for Shiseido in China in the future

Yoctonewton force detection based on optically levitated oscillator

Optically levitated oscillators in high vacuum have excellent environmental isolation and low mass compared with conventional solid-state sensors, which makes them suitable for ultrasensitive force detection. The force resolution usually scales with the measurement bandwidth, which represents the ultimate detection capability of the system under ideal conditions if sufficient time is provided for measurement. However, considering the stability of a real system, a method based on the Allan variance is more reliable to evaluate the actual force detection performance. In this study, a levitated optomechanical system with a force detection sensitivity of 6.33 ± 1.62 zN/Hz1/2 was demonstrated. And for the first time, the Allan variance was introduced to evaluate the system stability due to the force sensitivity fluctuations. The force detection resolution of 166.40 ± 55.48 yN was reached at the optimal measurement time of 2751 s. The system demonstrated in this work has the best force detection performance in both sensitivity and resolution that have been reported so far for optically levitated particles. The reported high-sensitivity force detection system is an excellent candidate for the exploration of new physics such as fifth force searching, high-frequency gravitational waves detection, dark matter research and so on

Stratification analysis of rs667282 genotypes by selected variables in ESCC patients and controls.

†<p>the smoker with CPD≤20.</p>‡<p>the smoker with CPD>20.</p>*<p>CI: confidence interval; OR: odds ratio.</p

The genotype distribution of 2 SNPs among cases and controls and their association with ESCC risk.

†<p>adjusted by age, gender, drinking status and smoking statusin logistic regression model. CI: confidence interval; OR: odds ratio.</p

Association between 2 SNPs genotype groups with histological grade and clinical stage of ESCC.

*<p>CI: confidence interval; OR: odds ratio.</p