Sustained swimming capacity of Atlantic salmon

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Heart rate bio-loggers as welfare indicators in Atlantic salmon (Salmo salar) aquaculture

In this study, 12 farmed Atlantic salmon (~1200 g) were tagged with commercially available heart rate (HR) bio-loggers and maintained in a controlled fish tank laboratory environment at 9 °C on a 12 h day/night cycle for 13 weeks. Apart from one fish that had obtained severe wounds on the tail region in the beginning, the remaining fish survived the entire test period and displayed consistent and similar HR in response to the day/night cycles with peak HR midday during feeding. At the end of the experiment, untagged conspecifics had significantly higher weights, fork lengths and conditions factors, showing the bio-logger may have a long term negative impact on growth. However, tagged fish still gained weight during the trial. Resting HR, as measured at night and early morning, decreased significantly over the first 2–3 weeks, and remained stable at ~25 beats min−1 between week 3 and 10, highlighting that substantial time is required for complete recovery following implantation of the bio-logger. At the start of week 11, 12 and 13, crowding stress trials of 30 min were performed which elevated HR to 55.7 beats min−1, whereafter it took 24 h to recover normal HR. Emerging bio-logger technologies can provide otherwise unobtainable information on the physiology and behaviour in free swimming individual fish over long periods and has great potential as welfare assessment tools in aquaculture. However, the impact of the tag must be considered with regards to the general representativeness of untagged counterparts when interpreting data.publishedVersio

What is The Limit of Sustained Swimming in Atlantic Salmon Post smolts?

A trend in Atlantic salmon aquaculture is to establish new production sites that are susceptible to extreme weather conditions; however, strong and persistent water currents may compromise fish welfare. Defining acceptable current conditions necessitates an assessment of sustained swimming abilities that are fuelled solely by aerobic metabolism and do not result in fatigue. In this study, the limit of sustained swimming was quantified with regards to both speed and time in Atlantic salmon of ~700 g and ~39 cm at 12°C by testing fish in groups of 10 in a large swim tunnel respirometer. First, critical swimming speed (Ucrit) (107 cm s-1) and minimum cost of transport (66 cm s-1) were measured. Sustained swimming trials at constant speeds were then performed based on a percentage of the group mean Ucrit (80, 85, 90, 95, 100 or 105%). Fish were forced to swim until they fatigued or until 72 h had passed. Surprisingly, most fish were able to sustain 80 and 85% Ucrit for 72 consecutive hours. However, at the highest speeds, fatigue was reached within the first 2 h. By categorizing fatigue times of individual fish into 72 h, significant differences in relative swimming speeds were found that corresponded to 2.7, 2.5 and 2.2 body lengths s-1, respectively. These results document impressive sustained swimming capacities in farmed Atlantic salmon and add important temporal insights about ambient current limits with regards to fish welfare at exposed aquaculture sites.publishedVersio

Fish welfare in offshore salmon aquaculture

To accommodate further growth in the Atlantic salmon aquaculture industry, new production sites may well be established at more exposed locations along the coast or even offshore. Here, fish will encounter strong currents and powerful waves, which are avoided at traditional sheltered locations. Exposed locations offer several advantages and necessitate new technological advancements. However, the most crucial question is whether Atlantic salmon are able to thrive in these more extreme environments. In this review, we describe how strong water currents affect the physiology, behaviour and ultimately the welfare of the fish. If ambient current speeds exceed swimming capacities, fish become fatigued and get stuck on the cage wall leading to unacceptable welfare. The swimming capacity will depend on both the magnitude and duration of the current speeds encountered. Moreover, several environmental and biological factors modulate swimming capabilities, where temperature, body size and health status are particularly important to consider. A series of empirical studies are subsequently used to formulate welfare guidelines with regard to biological limits in exposed aquaculture. In addition, owing to the growing popularity of cleaner fish in salmon aquaculture, we also evaluate their usefulness at exposed sites. Overall, Atlantic salmon is a powerful sustained swimmer, and based on available site surveys of ocean currents, we conclude that the prospects for responsible farming at exposed sites looks promising. However, cleaner fish species such as lumpfish and ballan wrasse are poor swimmers and are therefore not recommended for deployment at exposed sites.publishedVersio

Cage size affects dissolved oxygen distribution in salmon aquaculture

Atlantic salmon aquaculture is shifting toward larger cages, but the water quality implications of this shift are unknown. While larger cages could improve profitability through economies of scale, they may increase the risk of low dissolved O2 (DO) conditions due to reduced water exchange. Low DO conditions reduce feed intake, meaning that the benefits of shifting to larger cages must be weighed against potential negative impacts on fish growth. To test the impact of cage size on DO distribution, we recorded DO saturation in several circular cages of 2 different sizes on a commercial salmon farm: 6 with 168 m and 4 with 240 m circumference. Static strings of DO loggers at 1, 4.5, 8, 12 and 16 m depths recorded DO saturation once every 60 s throughout a 10 d period in mid-summer. Overall, DO levels in standard 168 m circumference cages were suitable for salmon feeding and growth. DO levels were highly variable (57 to 134% saturation), and were lower in cages than at the reference site. On average, DO saturation decreased with depth, and was lowest during the early morning hours. Lowest DO measurements occurred in the large 240 m circumference cages, where 1 in 20 of all recordings were at levels known to reduce salmon feeding and growth. DO levels in larger cages can suit salmon production, but site-specific environmental conditions throughout the year must be considered to ensure there is sufficient capacity to tolerate reduced water exchange.publishedVersio

The metabolic rate response to feed withdrawal in Atlantic salmon post-smolts

Feed withdrawal is a widespread practice in Atlantic salmon (Salmo salar) aquaculture to empty the gut prior to major farming operations, while certain pathogens and suboptimal environmental conditions in production cages are known to induce prolonged fasting. However, these fasting periods may be in conflict with ethical and legal obligations to farm animals. Presently, science-based recommendations on responsible fasting times that consider fish welfare are lacking. In this study, we measured the standard metabolic rate (SMR) and metabolic rate following acute handling and confinement stress in Atlantic salmon post smolts (~575 g, ~38 cm) following 1, 2, 3 and 4 weeks of feed withdrawal and 1 week of subsequent refeeding at 12 °C. The purpose was to identify when changes in metabolic mode occurred and assess whether the capacity to respond to stress eventually was compromised, since such observations could serve as potential welfare indicators. The SMR decreased significantly from 84.4 ± 4.7 mg O2 kg h−1 in control fish to 71.0 ± 1.8 mg O2 kg h−1 following 1 week of fasting. A further significant decrease to 65.0 ± 3.7 mg O2 kg h−1 was measured after 3 weeks, while refeeding returned SMR to control levels. The increase in MO2 following acute stress was unaffected for the first three weeks of fasting. However, the 4 week group showed a reduced peak response compared to the preceding weeks (278 ± 13 vs. 310 ± 7 mg O2 kg h−1). Weight, fork length and condition factor did not change significantly during the fasting period, and the fish immediately resumed eating upon refeeding. We conclude that up to 4 weeks of feed withdrawal had negligible effects on fish welfare. Moreover, an improved aerobic scope owing to a reduced SMR may be advantageous prior to certain farm operations.publishedVersio

Utvikling av lakselus ved ulik temperatur og lys - TEMPLUS

Lakselus påvirkes sterkt av miljøfaktorer som sjøvannets temperatur og saltholdighet. Sjøvannstemperaturen langs varierer sterkt avhengig av sesong og geografisk plassering. Saltholdighet utviser også store forskjeller lokalt, spesielt i fjorder med stort innslag av ferskvann. Formålet med dette prosjektet var å a) undersøke temperaturens innflytelse på utviklingsprosesser i lakselus på fisk og b) undersøke innflytelsen av saltholdighet og temperatur på adferden til frittlevende larvestadier. Alle forsøk ble utført i kar med lakselus samlet fra vestkysten av Norge. Resultatene viser tydelig at temperaturen er en sterk regulator av alle undersøkte biologiske prosesser i lakselus, med raskere utvikling, høyere reproduksjon og større infeksjonssuksess funnet ved høye temperaturer. Både saltholdighet og temperatur har også stor innflytelse på larveadferd, med forskjellige effekt på naupliuslarver og de smittsomme kopepoditter. Resultatene som er oppnådd, kan brukes til å predikere utvikling av lakselus i akvakultur og i laboratoriet, og gi ny informasjon om forvaltningen av parasitten ved lave temperaturer. Resultatene på larveadferd vil forbedre modellering av smittsomme kopepoditter og hjelpemiddel i utforming og bruk av forebyggende tiltak som brukes i oppdrettsanlegg.publishedVersio

Where art thou louse? A snapshot of attachment location preferences in salmon lice on Atlantic salmon hosts in sea cages

Problematic sea lice infestations on farmed Atlantic salmon (Salmo salar) have motivated extensive research and development into new methods to prevent, monitor and control sea lice. Most of these technologies require detailed information on the behaviour, spatial distribution and demography of lice on host fish. This study investigated how salmon lice (Lepeophtheirus salmonis) infestation density varies across the host's surface under sea cage farming conditions. Lice abundance, demography and attachment location were tracked over time, with repeated sampling of 300 individually tagged salmon across three replicate experimental sea cages. The data reveal clear differences in attachment locations according to sex and stage, but with an overall preference for the dorsal surface among mobile stages—dorsal head for adult females and dorsal‐posterior section for males and pre‐adults. Total lice abundance was highly variable between repeated measures of individual fish, consistent with frequent host‐switching or mortality. Total lice numbers also declined between sampling dates, likely due to handling, with lost mobile lice being almost exclusively adult males. As the distribution of sea lice on hosts is likely determined by numerous factors, future image‐based automated detection systems should be validated in settings that reflect the complex host–parasite interactions that occur in open farming systems.publishedVersio

Sea lice prevention strategies affect cleaner fish delousing efficacy in commercial Atlantic salmon sea-cages

Over the last 2 decades, cleaner fishes have been employed to remove external sea lice parasites from Atlantic salmon Salmo salar in sea cages. Norway, Scotland, Ireland, and the Faroe Islands combined now use ~60 million cleaner fish per year. While small-scale experiments demonstrate the efficacy of cleaner fishes, industrial-scale sea cages have multiple structures and conditions that create different environments, which may impact cleaner fish efficacy and welfare. Here, in commercial sea cages, we investigated if 4 different anti-lice strategies impacted the delousing efficacy, physical condition, and behaviour of cleaner fish (corkwing wrasse Symphodus melops). The strategies tested were: (1) cleaner fishes only; (2) cleaner fishes and functional feed; (3) cleaner fishes, functional feed, and deep lights and feeding; and (4) cleaner fishes, functional feed, deep lights and feeding, and lice skirts. Corkwing wrasse were sampled from 3 cage-level replicates of each anti-lice strategy 3 times over 2 mo. Lice levels on salmon were recorded every 3 to 4 wk. Only 11% of corkwing wrasse had salmon lice in their gut, with individual wrasse having up to 72 lice in their stomach. Wrasse in cages encircled by lice skirts consumed one-ninth as many lice as those in other anti-lice treatments and had less overall impact on the number of lice per salmon. Fin, skin, mouth and eye condition, K factor, and observed cleaning behaviours of corkwing wrasse were similar across all anti-lice strategies. Our results demonstrate that different in-cage anti-lice strategies altered the magnitude of lice consumption in corkwing wrasse at this site and for this production period. Moreover, while a small proportion of corkwing wrasse appear to target lice as prey, most individual corkwing wrasse were ineffective biological control agents in a full-scale farm setting.publishedVersio