Structural characterization of proteoglycans produced by testicular peritubular cells and Sertoli cells

The structural characteristics of proteoglycans produced by seminiferous peritubular cells and by Sertoli cells are defined. Peritubular cells secrete two proteoglycans designated PC I and PC II. PC I is a high molecular mass protein containing chondroitin glycosaminoglycan (GAG) chains (maximum 70 kDa). PC II has a protein core of 45 kDa and also contains chondroitin GAG chains (maximum 70 kDa). Preliminary results imply that PC II may be a degraded or processed form of PC I. A cellular proteoglycan associated with the peritubular cells is described which has properties similar to those of PC I. Sertoli cells secrete two different proteoglycans, designated SC I and SC II. SC I is a large protein containing both chondroitin (maximum 62 kDa) and heparin (maximum 15 kDa) GAG chains. Results obtained suggest that this novel proteoglycan contains both chondroitin and heparin GAG chains bound to the same core protein. SC II has a 50-kDa protein core and contains chondroitin (maximum 25 kDa) GAG chains. A proteoglycan obtained from extracts of Sertoli cells is described which contains heparin (maximum 48 kDa) GAG chains. In addition, Sertoli cells secrete a sulfoprotein, SC III, which is not a proteoglycan. SC III has properties similar to those of a major Sertoli cell-secreted protein previously defined as a dimeric acidic glycoprotein. The stimulation by follicle-stimulating hormone of the incorporation of [35S]SO2(-4) into moieties secreted by Sertoli cells is shown to represent an increased production or sulfation of SC III (i.e. dimeric acidic glycoprotein), and not an increased production or sulfation of proteoglycans. Results are discussed in relation to the possible functions of proteoglycans in the seminiferous tubule

Detailed Abundances for the Old Population near the Galactic Center: I. Metallicity distribution of the Nuclear Star Cluster

We report the first high spectral resolution study of 17 M giants kinematically confirmed to lie within a few parsecs of the Galactic Center, using R=24,000 spectroscopy from Keck/NIRSPEC and a new linelist for the infrared K band. We consider their luminosities and kinematics, which classify these stars as members of the older stellar population and the central cluster. We find a median metallicity of =-0.16 and a large spread from approximately -0.3 to +0.3 (quartiles). We find that the highest metallicities are [Fe/H]<+0.6, with most of the stars being at or below the Solar iron abundance. The abundances and the abundance distribution strongly resembles that of the Galactic bulge rather than disk or halo; in our small sample we find no statistical evidence for a dependence of velocity dispersion on metallicity.Comment: 18 pages, 14 figures, accepted for publication in A

Atmospheric Effects on Fate of Aerially Applied Agricultural Sprays

The deposition and drift of aerially applied crop protection materials is influenced by a numberof factors including equipment setup and operational parameters, spray material, and meteorological conditions. This work focuses on evaluating the meteorological influences on the transport and ultimate fate of aerially applied sprays. There was no single meteorological factor that dominated the downwind transport of the spray treatments replicated in this study. Generally, lower relative humidity decreased downwind deposition and the amount of spray unaccounted for due to evaporative effects. Increasing wind speeds decreased both in-swath deposition and downwind deposition, and increased the amount of mass unaccounted for.Increases in stability were only moderately correlated to downwind deposition and to flux measurements past 40 m. Though this data set covers a limited range of meteorological conditions, the trends hold from the standpoint of the system physics, and provide applicators with a further understanding of the relationships between spray transport and deposition and local meteorology

Tsirelson's problem and Kirchberg's conjecture

Tsirelson's problem asks whether the set of nonlocal quantum correlations with a tensor product structure for the Hilbert space coincides with the one where only commutativity between observables located at different sites is assumed. Here it is shown that Kirchberg's QWEP conjecture on tensor products of C*-algebras would imply a positive answer to this question for all bipartite scenarios. This remains true also if one considers not only spatial correlations, but also spatiotemporal correlations, where each party is allowed to apply their measurements in temporal succession; we provide an example of a state together with observables such that ordinary spatial correlations are local, while the spatiotemporal correlations reveal nonlocality. Moreover, we find an extended version of Tsirelson's problem which, for each nontrivial Bell scenario, is equivalent to the QWEP conjecture. This extended version can be conveniently formulated in terms of steering the system of a third party. Finally, a comprehensive mathematical appendix offers background material on complete positivity, tensor products of C*-algebras, group C*-algebras, and some simple reformulations of the QWEP conjecture.Comment: 57 pages, to appear in Rev. Math. Phy

Current status and future trends of precision agricultural aviation technologies

Modern technologies and information tools can be used to maximize agricultural aviation productivity allowing for precision application of agrochemical products. This paper reviews and summarizes the state-of-the-art in precision agricultural aviation technology highlighting remote sensing, aerial spraying and ground verification technologies. Further, the authors forecast the future of precision agricultural aviation technology with key development directions in precision agricultural aviation technologies, such as real-time image processing, variable-rate spraying, multi-sensor data fusion and RTK differential positioning, and other supporting technologies for UAV-based aerial spraying. This review is expected to provide references for peers by summarizing the history and achievements, and encourage further development of precision agricultural aviation technologies

Fibronectin Synthesis is a Marker for Peritubular Cell Contaminants in Sertoli Cell-Enriched Cultures

With indirect immunofluorescent microscopic techniques, we have shown that fibronectin is distributed primarily in or along the basal lamina of the seminiferous tubule boundary tissue in sections of testes from 20-day-old rats. Purified rat Sertoli cell-enriched aggregates, maintained in culture in the presence or absence of serum, exhibit no detectable immunofluorescence with fibronectin antibody, whereas purified peritubular cells in culture do have a positive reaction to fibronectin antibody. Peritubular cells in culture incorporate [35S] methionine into fibronectin which can be immunoprecipitated with a fibronectin antiserum, but Sertoli cells do not. We have used various criteria to estimate the degree of purity of Sertoli cell-enriched preparations. The presence of peritubular myoid cells in conventional Sertoli cell-enriched aggregates, cultured in the presence or absence of serum, can be detected with transmission electron microscopic examination, by the Feulgen staining procedure, and by the immunocytochemical identification of fibronectin. We describe a technique to purify Sertoli cells in conventional Sertoli cell-enriched preparations by treatment with hyaluronidase, resulting in a lesser number of peritubular cells by the above criteria, even in preparations cultured in the presence of serum. Data presented suggest that some of the products previously attributed exclusively to Sertoli cells in Sertoli cell-enriched preparations, particularly those cultured in the presence of serum, may have been contributed by peritubular cells