First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid- chromatography–Urgent need for harmonisation of analytical methods

Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5α-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys, each laboratory used its own internal standard (5α-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs

Simulating the role of tides and sediment characteristics on tidal flat sorting and bedding dynamics

Understanding sediment sorting and bedding dynamics has high value to unravelling the mechanisms underlying geomorphological, geological, ecological and environmental imprints of tidal wetlands and hence to predicting their future changes. Using the Nanhui tidal flat on the Changjiang (Yangtze) Delta, China, as a reference site, this study establishes a schematized morphodynamic model coupling flow, sediment dynamics and bed level change to explore the processes that govern sediment sorting and bedding phenomena. Model results indicate an overall agreement with field data in terms of tidal current velocities, suspended sediment concentrations (SSCs), deposition thicknesses and sedimentary structures. Depending on the variation of tidal current strength, sand-dominated layers (SDLs) and mud-dominated layers (MDLs) tend to form during spring and neap tides, respectively. Thinner tidal couplets are developed during daily scale flood–ebb variations. A larger tidal level variation during a spring–neap tidal cycle, associated with a stronger tidal current variation, favours the formation of SDLs and tidal couplets. A larger boundary sediment supply generally promotes the formation of tidal bedding, though the bedding detail is partially dependent on the SSC composition of different sediment types. Sediment properties, including for example grain size and settling velocity, are also found to influence sediment sorting and bedding characteristics. In particular, finer and coarser sediment respond differently to spring and neap tides. During neap tides, relatively small flow velocities favour the deposition of finer sediment, with limited coarser sediment being transported to the upper tidal flat because of the larger settling velocity. During spring tides, larger flow velocities transport more coarser sediment to the upper tidal flat, accounting for distinct lamination formation. Model results are qualitatively consistent with field observations, but the role of waves, biological processes and alongshore currents needs to be included in further studies to establish a more complete understanding.</p

An integrated approach to mangrove dynamics and management

The main objective of the MADAM project (Mangrove Dynamics and Management) is to generate the scientific basis enabling the sustainable stewardship of the resources of the Caeté mangrove estuary in Northeast Brazil in the sense of integrated coastal (zone) management. To achieve this, it is necessary to acquire in-depth knowledge of natural processes as well as of the relevant institutional, cultural, economic, social and political dynamics. Causal linkages within the ecosystem, as well as between ecosystem, economy and society, are analysed and explained via dynamic and trophic modelling. Scenario construction is intended to forecast the effects of acute or chronic interference on utilized resources, and to answer wider, management-related questions (e.g. restoration of destroyed areas, utilization potential for aquaculture). This paper describes the project strategy as developed and modified in the context of research results from the initial 2-yr project phase. It is argued that a continuous discussion process is essential to assess the validity of the strategies formulated at the beginning of a medium-time project, particularly if the project is of interdisciplinary nature