CD4+ Th immunogenicity of the Ascaris spp. secreted products

Ascaris spp. is a major health problem of humans and animals alike, and understanding the immunogenicity of its antigens is required for developing urgently needed vaccines. The parasite-secreted products represent the most relevant, yet complex (>250 proteins) antigens of Ascaris spp. as defining the pathogen-host interplay. We applied an in vitro antigen processing system coupled to quantitative proteomics to identify potential CD4+ Th cell epitopes in Ascaris-secreted products. This approach considerably restricts the theoretical list of epitopes using conventional CD4+ Th cell epitope prediction tools. We demonstrate the specificity and utility of our approach on two sets of candidate lists, allowing us identifying hits excluded by either one or both computational methods. More importantly, one of the candidates identified experimentally, clearly demonstrates the presence of pathogen-reactive T cells in healthy human individuals against these antigens. Thus, our work pipeline identifies the first human T cell epitope against Ascaris spp. and represents an easily adaptable platform for characterization of complex antigens, in particular for those pathogens that are not easily amenable for in vivo experimental validation

Validation of the Human Activity Profile Questionnaire in Patients after Allogeneic Hematopoietic Stem Cell Transplantation

Chronic graft-versus-host disease (cGVHD) associated morbidity and mortality remain major barriers for successful allogeneic hematopoietic stem cell transplantation (alloHSCT). Currently, no reliable measures are established to monitor cGVHD activity changes for use in clinical trials. The Human Activity Profile (HAP) patient self-report was proposed by the National Institutes of Health (NIH) cGVHD consensus project as an independent measure of patients' functional status that could also indirectly reflect improvement of cGVHD, but that has not been validated in an alloHSCT patient population. One hundred seventy-six patients (median age 44 years [range: 18-72 years] after alloHSCT were evaluated with a German translation of the HAP, the NIH criteria-based cGVHD activity assessment, the Lee cGVHD Symptom-Scale, FACT-BMT, SF36, Berlin Social Support Scale, 24-Item Adjective Measure (24-AM), Hospital Anxiety and Depression Scale, and the NCCN-Distress-Thermometer. Enrollment occurred a median of 286 (range: 85-4003) days after alloHSCT. Follow-up surveys were conducted at 1, 2, 3, 5, 8, and 12 months after the baseline survey. Although 117 patient had cGVHD at time of enrollment (mild n = 33, moderate n = 50, or severe n = 34), 59 patients were included into the study in the absence of cGVHD between days 85 and 395 after transplantation. The maximum activity score (MAS) and adjusted activity score (AAS) of the HAP correlated inversely with grading of cGVHD severity (mild, moderate, or severe) (r = −0.25 for MAS and −0.24 for AAS). Lung manifestations of cGVHD correlated with AAS (r = 0.17), but not with MAS. HAP scores correlated with subscales from other instruments measuring physical domains, especially the physical functioning scale of the SF36. Performance was improved by use of an HSCT-modified HAP scoring system that excluded activities prohibited within the first year after alloHSCT. No significant correlation of the HAP was found with personality, age, sex, symptom burden, or social functioning or social well-being. Moreover, the HAP displayed a higher sensitivity to change of cGVHD activity compared to the SF36 and the FACT-BMT. In addition, steroid myopathy correlated with both HAP scores, but not the SF36. The HAP is a simple and valid questionnaire for the evaluation of the physical activity in patients after alloHSCT, with the advantage of detecting changes in cGVHD status independently of other quality-of-life measures and with a superior sensitivity compared to the SF36

Dental therapy before and after radiotherapy–an evaluation on patients with head and neck malignancies

The present investigation evaluates the dental care situation of patients with head and neck cancer before and after radiotherapy. The situations of these patients in 1993 and 2005 were compared to detect similarities, differences and developments. In the years 1993 and 2005, 37 and 36 patients, respectively, with head and neck cancer treated by the local departments of otorhinolaryngology and of radiotherapy were examined consecutively according to their aftercare appointments. Time points of radiotherapy treatment of the patients evaluated in 1993 varied from 1984 to 1993. The patients evaluated in 2005 had received radiotherapy between 1998 and 2005. Therefore the applied radiotherapeutic regimen differed not only between the two groups of patients, but also within each group. The information for these investigations was provided anonymously. It was evaluated with descriptive statistics. The evaluation of the data shows distinct differences with respect to preventive and therapeutic dental care measures. In 2005, 35 out of 36 patients (97.2%) had a dental consultation before radiotherapy (1993, 65%). All 27 dentate patients (100%) obtained a splint for fluoride application (1993, none). 29% fewer edentulous patients were seen than in 1993. The number of teeth destroyed decreased from 19.2% (1993) to 7.8% in 2005. Mycoses due to Candida spp. and chronic failures in wound healing were rare (5.5%). In the course of the 12 years, prophylactic measures, such as the application of splints for fluoride treatment, were intensified. However, concepts for the dental care of patients undergoing radiotherapy, especially following the radiation, should be widened to avoid ruined teeth and long delayed wound healings

Paleo‐Hydrogeological Modeling to Understand Present‐Day Groundwater Salinities in a Low‐Lying Coastal Groundwater System (Northwestern Germany)

Groundwater abstraction and drainage are considered to be the main drivers for the salinization of low‐lying coastal groundwater systems, while the role of past boundary conditions is less clear. In this study, 3‐D paleo‐hydrogeological variable‐density groundwater flow and salt transport modeling (“paleo‐modeling”) is applied to reconstruct the evolution of groundwater salinities during the Holocene, that is, the last 9,000 years, in Northwestern Germany. Novel aspects of this study include the consideration of highly resolved time‐variant boundary conditions in a 3‐D paleo‐modeling framework, for example, sea‐level rise, surface elevation and coastline changes, development of drainage networks and groundwater abstraction, as well as the quantification of isolated processes impacting salinization. Results show that salinization was a function of sea‐level rise from 9000 BP until 1300 CE. The creation of the dike line ∼1300 CE set the starting point for increasing anthropogenic control of the hydro(geo)logical system: changes in surface elevation and drainage of low‐lying marshes have become main drivers for salinization after 1600 CE when peat was artificially degenerated. Moreover, changes in the dike line caused by storm floods impacted the salinities. Model results for 2020 CE match well with present‐day salinity observations. Yet, salinization will continue in the future, as the hydro(geo)logical system has not reached an equilibrium. The presented paleo‐modeling framework can be viewed as a blueprint for similar low‐lying coastal groundwater systems, influenced by marine transgression and human development. Thereby, it enables the reconstruction of meaningful present‐day salinity distributions, serving as a vital basis for modeling future groundwater systems in a changing climate.Key Points: Salinization of a low‐lying coastal groundwater system during the Holocene, that is, 9000 BP until present‐day, was investigated. Sea‐level rise and the evolution of paleogeography were major controls on groundwater salinization during most of the time. Surface elevation changes due to land cultivation, as well as the development of drainage networks, control salinization since ∼1600 CE.Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659Bundesministerium für Bildung und Forschung http://dx.doi.org/10.13039/501100002347https://doi.org/10.5281/zenodo.763338

Link technology for all-optical satellite-based quantum key distribution system in C-/L-band

Satellite based quantum key distribution (QKD) enables the delivery of keys for quantum secure communications over long distances. Maturity of the technology as well as industrial interest keep increasing. So does the technology readiness of satellite free-space optical communications. A satellite QKD system comprises a quantum communication subsystem and a classical communication subsystem (public channel). Both are implemented with free-space optics. Thus, in satellite QKD system design, there are strong synergies that should be exploited as much as possible and lead to an all-optical satellite QKD system. In this paper, we present a system like this locating all optical channels in ITU DWDM C-band. We focus on the overall conceptual design and the setup of the optical channels for quantum and classical signal transmission. The system description addresses the breadboards of a transmitter laser terminal (Alice terminal), a receiver laser terminal, (Bob terminal), the public channel implementation, the interfaced QKD system and the deployed encryption system. The design basis for the Alice terminal is the laser terminal development OSIRISv3. The design basis for the Bob terminal is the ground station development THRUST. The later contains an adaptive optics correction to enable single mode fiber coupling. This enables the interfacing to almost arbitrary quantum receivers such as the Bob modules used in the described experiment. The public channel is composed of a bi-directional 1 Gbps IM/DD system and a MODEM that implements a proprietary waveform optimized for free-space channels