Plant biomass nitrogen and effects on the risk of nitrate leaching of intercrops under organic farming in Eastern Austria

Data on the potential of intercrops to reduce soil nitrate contents, on their N accumulation and biological nitrogen fixation (BNF) are lacking for organic farming in the dry, pannonic region of Eastern Austria. The effect of legumes, non-legumes, and legumes + non-legumes used as intercrops on inorganic soil nitrogen, crop yield and biomass N, and BNF were tested in comparison to bare fallow. Non-legumes and legumes + non-legumes were more efficient than legumes in reducing inorganic soil N contents in autumn and nitrate contents in soil solution from the subsoil in winter. This reduction in inorganic soil N did not last until March of the following year due to an N mineralisation from the mulch. The legume + non-legume mixture contained a larger amount of crop N than both legumes and non-legumes. This was due to the combined effect of soil-N uptake by the non-legumes and BNF by the legumes in the mixture

preclinical evidence from C57BL/6 mice

Chemotherapy-induced central nervous system (CNS) neurotoxicity presents an unmet medical need. Patients often report a cognitive decline in temporal correlation to chemotherapy, particularly for hippocampus-dependent verbal and visuo-spatial abilities. We treated adult C57Bl/6 mice with 12 × 20 mg kg−1 paclitaxel (PTX), mimicking clinical conditions of dose-dense chemotherapy, followed by a pulse of bromodesoxyuridine (BrdU) to label dividing cells. In this model, mice developed visuo-spatial memory impairments, and we measured peak PTX concentrations in the hippocampus of 230 nm l−1, which was sevenfold higher compared with the neocortex. Histologic analysis revealed a reduced hippocampal cell proliferation. In vitro, we observed severe toxicity in slowly proliferating neural stem cells (NSC) as well as human neuronal progenitor cells after 2 h exposure to low nanomolar concentrations of PTX. In comparison, mature post-mitotic hippocampal neurons and cell lines of malignant cells were less vulnerable. In PTX-treated NSC, we observed an increase of intracellular calcium levels, as well as an increased activity of calpain- and caspase 3/7, suggesting a calcium-dependent mechanism. This cell death pathway could be specifically inhibited with lithium, but not glycogen synthase kinase 3 inhibitors, which protected NSC in vitro. In vivo, preemptive treatment of mice with lithium prevented PTX-induced memory deficits and abnormal adult hippocampal neurogenesis. In summary, we identified a molecular pathomechanism, which invokes PTX-induced cytotoxicity in NSC independent of cell cycle status. This pathway could be pharmacologically inhibited with lithium without impairing paclitaxel’s tubulin-dependent cytostatic mode of action, enabling a potential translational clinical approach

Interplay between distribution of live cells and growth dynamics of solid tumours

Experiments show that simple diffusion of nutrients and waste molecules is not sufficient to explain the typical multilayered structure of solid tumours, where an outer rim of proliferating cells surrounds a layer of quiescent but viable cells and a central necrotic region. These experiments challenge models of tumour growth based exclusively on diffusion. Here we propose a model of tumour growth that incorporates the volume dynamics and the distribution of cells within the viable cell rim. The model is suggested by in silico experiments and is validated using in vitro data. The results correlate with in vivo data as well, and the model can be used to support experimental and clinical oncology

Stickstoffaufnahme und Biomasseertrag von Zwischenfrüchten und deren Auswirkungen auf Bodennitratgehalte und die Folgekultur unter den Bedingungen des Ökologischen Landbaus im pannonischen Klimagebiet

Data on the potential of catch crops to reduce soil nitrate contents, on their N accumulation and biological nitrogen fixation (BNF) are lacking for organic farming in the dry, pannonic region of Eastern Austria. The effect of legume, non-legume, and legume + non-legume crops used as catch crops on inorganic soil nitrogen, crop biomass and crop N, and BNF were tested in comparison to bare fallow. Non-legumes and legumes + non-legumes were more efficient than legumes in reducing inorganic soil N contents in autumn and nitrate contents in soil solution from the subsoil in winter. This reduction in inorganic soil N did not last until March of the following year due to N mineralisation from the mulch. The legume + non-legume mixture contained a larger amount of crop N than both legumes and non-legumes. This was due to the combined effect of soil-N uptake by the non-legumes and BNF by the legumes in the mixture

Massive stars and the energy balance of the interstellar medium. II. The 35 solar mass star and a solution to the "missing wind problem"

We continue our numerical analysis of the morphological and energetic influence of massive stars on their ambient interstellar medium for a 35 solar mass star that evolves from the main sequence through red supergiant and Wolf-Rayet phases, until it ultimately explodes as a supernova. We find that structure formation in the circumstellar gas during the early main-sequence evolution occurs as in the 60 solar mass case but is much less pronounced because of the lower mechanical wind luminosity of the star. Since on the other hand the shell-like structure of the HII region is largely preserved, effects that rely on this symmetry become more important. At the end of the stellar lifetime 1% of the energy released as Lyman continuum radiation and stellar wind has been transferred to the circumstellar gas. From this fraction 10% is kinetic energy of bulk motion, 36% is thermal energy, and the remaining 54% is ionization energy of hydrogen. The sweeping up of the slow red supergiant wind by the fast Wolf-Rayet wind produces remarkable morphological structures and emission signatures, which are compared with existing observations of the Wolf-Rayet bubble S308. Our model reproduces the correct order of magnitude of observed X-ray luminosity, the temperature of the emitting plasma as well as the limb brightening of the intensity profile. This is remarkable, because current analytical and numerical models of Wolf-Rayet bubbles fail to consistently explain these features. A key result is that almost the entire X-ray emission in this stage comes from the shell of red supergiant wind swept up by the shocked Wolf-Rayet wind rather than from the shocked Wolf-Rayet wind itself as hitherto assumed and modeled. This offers a possible solution to what is called the ``missing wind problem'' of Wolf-Rayet bubbles.Comment: 52 pages, 20 figures, 2 tables, accepted for publication in the Astrophysical Journa

Wasserhaushaltsmessungen in ökologisch bewirtschafteten Leguminosenbeständen im Trockengebiet Ostösterreichs

Durch die Kombination von Feldmessungen und Simulation werden Änderungen im Bodenwassergehalt erfasst und Wasserbilanzen errechnet. Es ist vorgesehen in vier Plots (Meßprofile) die Bodenwasserspannung und den Wasseranteil online zu erfassen. Diese Profile dienen zur Erfassung der zeitlichen Auflösung an einem Standort und werden auch für die Kalibrierung der Simulation herangezogen. Die Simulation bietet die Möglichkeit unterschiedliche Szenerien zu bearbeiten und Sensibilitätsanalysen durchzuführen. Die Kombination der Messung und der Simulation ist eine sehr kostengünstige und flexible Vorgangsweise zur umfassenden Beschreibung der Stoffumsetzungsprozesse im Boden unter verschiedenen Bedingunge

Azobenzene-functionalized alkanethiols in self-assembled monolayers on gold

Self-assembledmonolayers (SAMs) of 4-trifluoromethyl-azobenzene-4'-methyleneoxy-alkanethiols (CF3–C6H4–N=N–C6H4–O–(CH2) n–SH on (111)-oriented polycrystalline gold films on mica were examined by X-ray photoelectron spectroscopy (XPS) and X-ray absorption spectroscopy (XAS). The spectra are analyzed with the help of density-functional-theory calculations of the isolated molecule. Only one doublet is detected in the sulphur 2p spectra of the investigated SAMs, consistent with a thiolate bond of the molecule to the gold surface. The C 1s XP spectra and the corresponding XAS π* resonance exhibit a rich structure which is assigned to the carbon atoms in the different chemical surroundings. Comparing XPS binding energies of the azobenzene moiety and calculated initial-state shifts reveals comparable screening of all C 1s core holes. While the carbon 1s XPS binding energy lies below the π*-resonance excitation-energy, the reversed order is found comparing core ionization and neutral core excitation of the nitrogen 1s core-hole of the azo group. This surprising difference in core-hole binding energies is interpreted as site-dependent polarization screening and charge transfer among the densely packed aromatic moieties. We propose that a quenching of the optical excitation within the molecular layer is thus one major reason for the low trans to cis photo-isomerization rate of azobenzene in romaticaliphatic SAMs

Ranking ligand affinity for the DNA minor groove by experiment and simulation

The structural and thermodynamic basis for the strength and selectivity of the interactions of minor-groove binders (MGBs) with DNA is not fully understood. In 2003 we reported the first example of a thiazole containing MGB that bound in a phase shifted pattern that spanned 6 base-pairs rather than the usual 4 (for tricyclic distamycin-like compounds). Since then, using DNA footprinting, nuclear magnetic resonance spectroscopy, isothermal titration calorimetry and molecular dynamics, we have established that the flanking bases around the central 4 being read by the ligand have subtle effects on recognition. We have investigated the effect of these flanking sequences on binding and the reasons for the differences and established a computational method to rank ligand affinity against varying DNA sequences

A phenomenological approach to the simulation of metabolism and proliferation dynamics of large tumour cell populations

A major goal of modern computational biology is to simulate the collective behaviour of large cell populations starting from the intricate web of molecular interactions occurring at the microscopic level. In this paper we describe a simplified model of cell metabolism, growth and proliferation, suitable for inclusion in a multicell simulator, now under development (Chignola R and Milotti E 2004 Physica A 338 261-6). Nutrients regulate the proliferation dynamics of tumor cells which adapt their behaviour to respond to changes in the biochemical composition of the environment. This modeling of nutrient metabolism and cell cycle at a mesoscopic scale level leads to a continuous flow of information between the two disparate spatiotemporal scales of molecular and cellular dynamics that can be simulated with modern computers and tested experimentally.Comment: 58 pages, 7 figures, 3 tables, pdf onl