143 research outputs found
Lipid classes of mussel seeds Mytilus galloprovincialis of subtidal and rocky shore origin
15 pĂĄginas, 4 tablas, 1 figuraThe lipid class composition in juveniles of the mussel Mytilus galloprovincialis of rocky shore
and subtidal origin were compared after transfer to a subtidal environment in the Ria de Arousa
(northwest Spain). The experiment was conducted between November 27, 1995 and July 3, 1996. In
addition to mussel origin, the influence of the different environmental parameters on the changes in
lipid classes was studied. At the start of the experimental period, only the relative percentage of the
triacylglycerols (energetic function) was significantly higher in the subtidal specimens. However,
when the initial absolute contents were examined, the phospholipids and sterols were also
significantly higher in this mussel group. Differences in the relative percentages of phospholipids
and sterols were maintained until day 22 of the experiment. Our results show that during the first 36
days of the experimental period the mussel origin participated significantly in the model explaining
the variance of triacylglycerols, phospholipids and sterols. These results suggest the initial
differences in content and relative percentages of the lipid classes studied are possibly linked to the
contrasting environmental conditions in which the two mussel groups had previously developed
(subtidal and rocky shore habitats). In contrast, 50 days into the experiment the origin term did not
participate in the model of variance of these lipid classes. These results in turn suggest that during the
course of the investigation the mussel seeds of rocky shore origin were able to exploit the available
food resources in the subtidal habitat. Based on these results, the influence of mussel origin and environmental parameters on the changes in lipid classes of both mussel groups is discussed.This study was financed by the project CICYT MAR97-0592.Peer reviewe
Abundancia, DistribuciĂłn y DescripciĂłn de la PesquerĂa del Bivalvo Atrina seminuda en el Nororiente de Venezuela
Structure and hydration of membranes embedded with voltage-sensing domains.
Despite the growing number of atomic-resolution membrane protein structures, direct structural information about proteins in their native membrane environment is scarce. This problem is particularly relevant in the case of the highly charged S1-S4 voltage-sensing domains responsible for nerve impulses, where interactions with the lipid bilayer are critical for the function of voltage-activated ion channels. Here we use neutron diffraction, solid-state nuclear magnetic resonance (NMR) spectroscopy and molecular dynamics simulations to investigate the structure and hydration of bilayer membranes containing S1-S4 voltage-sensing domains. Our results show that voltage sensors adopt transmembrane orientations and cause a modest reshaping of the surrounding lipid bilayer, and that water molecules intimately interact with the protein within the membrane. These structural findings indicate that voltage sensors have evolved to interact with the lipid membrane while keeping energetic and structural perturbations to a minimum, and that water penetrates the membrane, to hydrate charged residues and shape the transmembrane electric field
Influence of environmental factors on the reproductive cycle of the eared ark Anadara notabilis (Röding, 1798) in Northeastern Venezuela
5 figuras, 2 tablasThe reproductive cycle of the eared ark Anadara notabilis and its relationship with environmental factors was
evaluated every 15 days between March 2004 and February 2005 at the northern part of the Peninsula de Araya, Venezuela.
Environmental factors measured included temperature, salinity, chlorophyll a, and total seston, including the particulate organic
matter and particulate inorganic matter fractions. Adult specimens were collected using a net drag and then randomly selected to
estimate wet live biomass and dry biomass of soft body tissues. Gonad samples were processed with histological analysis to
determine sex ratio, developmental stages, and variations of follicle size. Reproduction in A. notabilis was continuous throughout
the year, with spawning peaks occurring in June and October, coinciding with the lowest water temperatures. In March,
September, and November 2004, specimens attained the highest dry biomass values, whereas lowest dry biomass occurred in June
and October 2004. Stepwise regression analyses demonstrated that temperature and particulate organic matter values are the main
modulators of reproductive events. When temperature decreased, dribble spawning was detected in this species, possibly inducing
a survival strategy whereby the spawning period is extended to increase reproductive successPeer reviewe
Allosteric Mechanism of Water Channel Gating by Ca2+âcalmodulin
Calmodulin (CaM) is a universal regulatory protein that communicates the presence of calcium to its molecular targets and correspondingly modulates their function. This key signaling protein is important for controlling the activity of hundreds of membrane channels and transporters. However, our understanding of the structural mechanisms driving CaM regulation of full-length membrane proteins has remained elusive. In this study, we determined the pseudo-atomic structure of full-length mammalian aquaporin-0 (AQP0, Bos Taurus) in complex with CaM using electron microscopy to understand how this signaling protein modulates water channel function. Molecular dynamics and functional mutation studies reveal how CaM binding inhibits AQP0 water permeability by allosterically closing the cytoplasmic gate of AQP0. Our mechanistic model provides new insight, only possible in the context of the fully assembled channel, into how CaM regulates multimeric channels by facilitating cooperativity between adjacent subunits
Sympathetic Innervation of the Mammalian Pineal Gland: Its Involvement in Ontogeny and Physiology, and in Pineal Dysfunction
In mammals, the melatonin-producing pineal gland (PG) receives sympathetic innervation from the superior cervical ganglia (SCG). This chapter describes the role of this innervation on the PGâs ontogeny and rhythmic function, along with consequences to physiology when this regulation is disrupted. The PG and the SCG are components of the circadian timing system (CTS). Therefore, the overall CTS is described, including its oscillatory basis, its synchronization to the light: dark (L:D) cycles, and the dissemination of timing cues to all cells throughout the body. Pineal cellular composition and heterogeneity, cell-cell interactions, and the molecular mechanisms involved in the circadian rhythm of melatonin (MEL), are discussed. The SCGâs bilateral placement among surrounding anatomical landmarks, as well as their afferent and efferent connections, are described and illustrated. In addition, the SCG-related surgical models and the state-of-the art technology used to investigate the connection between SCG and PG are presented. Perspectives and gaps in our understanding are also discussed. We hope this chapter inspires readers to delve deeper into the field of the pineal gland and its main messenger, melatonin, as well as MELâs impact in health and disease, including as a remedial therapy
Comparative performance of the mussels Perna perna and Perna viridis, cultivated at four different depths
Multidrug-resistant Tuberculosis in Military Recruits
We conducted a tuberculosis contact investigation for a female military recruit with an unreported history of multidrug-resistant tuberculosis (MDRTB) and subsequent recurrence. Pertinent issues included identification of likely contacts from separate training phases, uncertainty on latent MDRTB infection treatment regimens and side effects, and subsequent dispersal of the contacts after exposure
Sustainability of bioenergy â Mapping the risks & benefits to inform future bioenergy systems
Bioenergy is widely included in energy strategies for its GHG mitigation potential. Bioenergy technologies will likely have to be deployed at scale to meet decarbonisation targets, and consequently biomass will have to be increasingly grown/mobilised. Sustainability risks associated with bioenergy may intensify with increasing deployment and where feedstocks are sourced through international trade. This research applies the Bioeconomy Sustainability Indicator Model (BSIM) to map and analyse the performance of bioenergy across 126 sustainability issues, evaluating 16 bioenergy case studies that reflect the breadth of biomass resources, technologies, energy vectors and bio-products. The research finds common trends in sustainability performance across projects that can inform bioenergy policy and decision making. Potential sustainability benefits are identified for People (jobs, skills, income, energy access); for Development (economy, energy, land utilisation); for Natural Systems (soil, heavy metals), and; for Climate Change (emissions, fuels). Also, consistent trends of sustainability risks where focus is required to ensure the viability of bioenergy projects, including for infrastructure, feedstock mobilisation, techno-economics and carbon stocks. Emission mitigation may be a primary objective for bioenergy, this research finds bioenergy projects can provide potential benefits far beyond emissions - there is an argument for supporting projects based on the ecosystem services and/or economic stimulation they may deliver. Also given the broad dynamics and characteristics of bioenergy projects, a rigid approach of assessing sustainability may be incompatible. Awarding âcreditâ across a broader range of sustainability indicators in addition to requiring minimum performances in key areas, may be more effective at ensuring bioenergy sustainability
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