The WAGGS project - II. The reliability of the calcium triplet as a metallicity indicator in integrated stellar light.

Using data from the WiFeS Atlas of Galactic Globular cluster Spectra, we study the behaviour of the calcium triplet (CaT), a popular metallicity indicator in extragalactic stellar population studies. A major caveat of these studies is that the potential sensitivity to other stellar population parameters such as age, calcium abundance, and the initial mass function has not yet been empirically evaluated. Here, we present measurements of the strength of the CaT feature for 113 globular clusters in the Milky Way and its satellite galaxies. We derive empirical calibrations between the CaT index and both the iron abundance ([Fe/H]) and calcium abundance ([Ca/H]), finding a tighter relationship for [Ca/H] than for [Fe/H]. For stellar populations, 3 Gyr and older the CaT can be used to reliably measure [Ca/H] at the 0.1 dex level but becomes less reliable for ages of ∼2 Gyr and younger. We find that the CaT is relatively insensitive to the horizontal branch morphology. The stellar mass function however affects the CaT strengths significantly only at low metallicities. Using our newly derived empirical calibration, we convert our measured CaT indices into [Ca/H] values for the globular clusters in our sample

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Extended-Range Undersea Laser Imaging: Current Research Status and a Glimpse at Future Technologies

AbstractRecent advancements in obtaining visibility of undersea objects at extended ranges in coastal and oceanic waters are reviewed for the years 2009 to present. The paper focuses on the latest techniques that are utilized to reduce the undesirable effects of scattering, mainly due to suspended particulate within the imaging volume, leading to the loss of contrast and blurring characteristic of undersea optical images produced over long ranges. Several recent sets of experimental results obtained using both benchtop laboratory development systems as well as field-deployable prototypes of new system concepts are presented, with observed performance attributes being discussed. Simulation studies that make use of accurate radiative transfer physical models to enable design and operation of new system concepts within a turbid water environment are also presented. Finally, this paper includes a description and results from an extended-range laser system that has reached a level of packaging and automation necessary to be available as a commercial product.</jats:p