Postgraduate Coursework Studies handbook

2002 handbook for Postgraduate Coursework Studie

Modular actin nano-architecture enables podosome protrusion and mechanosensing

Basement membrane transmigration during embryonal development, tissue homeostasis and tumor invasion relies on invadosomes, a collective term for invadopodia and podosomes. An adequate structural framework for this process is still missing. Here, we reveal the modular actin nano-architecture that enables podosome protrusion and mechanosensing. The podosome protrusive core contains a central branched actin module encased by a linear actin module, each harboring specific actin interactors and actin isoforms. From the core, two actin modules radiate: ventral filaments bound by vinculin and connected to the plasma membrane and dorsal interpodosomal filaments crosslinked by myosin IIA. On stiff substrates, the actin modules mediate long-range substrate exploration, associated with degradative behavior. On compliant substrates, the vinculin-bound ventral actin filaments shorten, resulting in short-range connectivity and a focally protrusive, non-degradative state. Our findings redefine podosome nanoscale architecture and reveal a paradigm for how actin modularity drives invadosome mechanosensing in cells that breach tissue boundaries

Ultrastructural immunogold labeling of lipid-laden enterocytes from patients with genetic malabsorption syndromes

Contains fulltext : 23285___.PDF (publisher's version ) (Open Access

Polarised expression of human intestinal N-benzoyl-l-tyroxyl-p-aminobenzoic acid hydrolase (human meprin) alpha and beta subunits in Madin Darby canine kidney cells

Contains fulltext : 24674___.PDF (publisher's version ) (Open Access

Detection of specific messenger RNA by electron microscopy in situ hybridization : implications for nucleocytoplasmic transport

Contains fulltext : 21773___.PDF (publisher's version ) (Open Access

Saponin pre-treatment in pre-embedding electron microscopic in situ hybridization for detection of specific RNA sequences in cultured cells : a methodological study

Contains fulltext : 21092___.PDF (publisher's version ) (Open Access

Role of negatively charged residues in the fifth and sixth transmembrane domains of the catalytic subunit of gastric H+,K+-ATPase

Contains fulltext : 28302.pdf (publisher's version ) (Open Access

Evaluation of pepsin treatment for electron microscopic RNA in situ hybridization on ultra-thin cryosections of cultured cells

Contains fulltext : 23853___.PDF (publisher's version ) (Open Access

Advantages of the use of a filmrecorder for the reproduction of digital TEM/SEM (and LM) images

Item does not contain fulltex