On the rate of convergence of the Hamiltonian particle-mesh method

The Hamiltonian Particle-Mesh (HPM) method is a particle-in-cell method for compressible fluid flow with Hamiltonian structure. We present a numer- ical short-time study of the rate of convergence of HPM in terms of its three main governing parameters. We find that the rate of convergence is much better than the best available theoretical estimates. Our results indicate that HPM performs best when the number of particles is on the order of the number of grid cells, the HPM global smoothing kernel has fast decay in Fourier space, and the HPM local interpolation kernel is a cubic spline

Leren van toezicht

Toezicht staat onder immer toenemende belangstelling, of het nu gaat om het toezicht op financiële instellingen, de veiligheid van het vliegverkeer of de gezondheidszorg. De oorzaken hiervan zijn divers maar kunnen deels gevonden worden in het terugtreden van de overheid als uitvoerder van publieke diensten. Om controle te kunnen houden op die diensten zijn de afgelopen decennia grote toezichthoudende organen opgericht, die inmiddels een flink deel van de economie beslaan; volgens sommige berekeningen werken momenteel ongeveer een miljoen mensen in de ‘toezichtsindustrie’ (van Waarden 2006). Daarnaast dringt steeds meer het besef door dat we leven in een ‘risicomaatschappij’ (Beck 1992) die, hoe paradoxaal ook, aanleiding heeft gegeven tot een ‘veiligheidsutopie’ (Boutelier 2002). Risico’s worden steeds minder geaccepteerd en overheden en toezichthouders worden daar in toenemende mate op aangesproken. Deze trend brengt met zich mee dat onderzoek naar het functioneren van toezichthouders sterk in maatschappelijk belang heeft gewonnen. De afgelopen jaren is dan ook internationaal te constateren dat dit onderzoek een hoge vlucht heeft genomen. Ook de inspecties zelf hebben inmiddels onderzoeksprogramma’s opgezet. Dit geldt ook voor de Inspectie voor de Gezondheidszorg (IGZ). Het voorliggende rapport is een verslag van onderzoek naar de effectiviteit van een van de belangrijke toezichtsinstrumenten van de IGZ, het zogeheten Thematisch Toezicht. Middels casestudie onderzoek beoogt dit rapport de vraag te beantwoorden op welke manier het thematisch toezicht effectief kan zijn en voor welk type risicoproblemen deze vorm van toezicht het meest geschikt is. Hiermee probeert het rapport een zowel conceptuele als empirische bijdrage te leveren aan de discussie over het toezicht in algemene zin, en in het bijzonder over het toezicht op en in de gezondheidszorg

DNA sequences required for regulated expression of β-globin genes in murine erythroleukaemia cells.

We introduced into MEL cells rabbit beta-globin gene deletion mutants and two sets of hybrid genes constructed from the inducible human beta-globin gene and noninducible human gamma-globin gene or the murine H-2Kbm1 class I MHC gene. S1 nuclease analysis of gene transcripts before and after MEL differentiation showed that induction of the rabbit beta-globin gene did not require more than 58 bp of DNA 5' to the transcription initiation site. Hybrid genes were constructed with human beta-globin DNA sequences from either 5' or 3' of the translation initiation site linked to the complementary parts of the gamma or H2Kbm1 genes. Both types of constructs were inducible during MEL differentiation. The relative rates of transcription of the 5' gamma-3' beta and 5'H2-3' beta hybrid genes show that induction of the hybrid gene transcripts results at least in part from transcriptional activation of the genes. We suggest that DNA sequences that regulate beta-globin gene transcription during MEL differentiation are located both 5' and 3' to the translation initiation site

The complete amino acid sequence of the antenna polypeptide B806-866-β from the cytoplasmic membrane of the green bacterium Chloroflexus auranliacus

AbstractThe bacteriochlorophyll a-binding polypeptide B806–866-β was extracted from membranes of the green thermophilic bacterium Chloroflexus aurantiacus with chloroform/methanol/ammonium acetate. Purification of the antenna polypeptide (6.3 kDa) was achieved by chromatography on Sephadex LH-60, Whatman DE-32 and by FPLC. The complete amino acid sequence (53 amino acid residues) was determined. The B806–866-β polypeptide is sequence homologous to the antenna β-polypeptides of purple bacteria (27–40%) and exhibits the characteristic three domain structure of the B870, B800–850 and B800–820 antenna complexes. The two typical His residues, conserved in all antenna β-polypeptides of purple bacteria, were found: His-24 lies within the N-terminal hydrophilic domain and His-42 within the central hydrophobic domain. This polypeptide together with the previously described α-polypeptide form the basic structural unit of the B806–866 antenna complex from C. aurantiacus

Fecundity compensation and tolerance to a sterilizing pathogen in <em>Daphnia</em>

Hosts are armed with several lines of defence in the battle against parasites: they may prevent the establishment of infection, reduce parasite growth once infected or persevere through mechanisms that reduce the damage caused by infection, called tolerance. Studies on tolerance in animals have focused on mortality, and sterility tolerance has not been investigated experimentally. Here, we tested for genetic variation in the multiple steps of defence when the invertebrate Daphnia magna is infected with the sterilizing bacterial pathogen Pasteuria ramosa: anti-infection resistance, anti-growth resistance and the ability to tolerate sterilization once infected. When exposed to nine doses of a genetically diverse pathogen inoculum, six host genotypes varied in their average susceptibility to infection and in their parasite loads once infected. How host fecundity changed with increasing parasite loads did not vary between genotypes, indicating that there was no genetic variation for this measure of fecundity tolerance. However, genotypes differed in their level of fecundity compensation under infection, and we discuss how, by increasing host fitness without targeting parasite densities, fecundity compensation is consistent with the functional definition of tolerance. Such infection-induced life-history shifts are not traditionally considered to be part of the immune response, but may crucially reduce harm (in terms of fitness loss) caused by disease, and are a distinct source of selection on pathogens

Isolation of β-globin related genes from a human cosmid library.

A human gene library was constructed using an improved cloning technique for cosmid vectors. Human placental DNA was partially digested with restriction endonuclease MboI; size-fractionated and ligated to BamHI-cut and phosphatase-treated cosmid vector pJB8. After packaging in lambda phage particles, the recombinant DNA was transduced into Escherichia coli 1400 or HB101 followed by selection on ampicillin for recombinant E. coli. 150 000 recombinant-DNA-containing colonies were screened for the presence of the human beta-globin related genes. Five recombinants were isolated containing the human beta-globin locus and encompassing approx. 70 kb of human DNA

Eigenvalue estimates for non-selfadjoint Dirac operators on the real line

We show that the non-embedded eigenvalues of the Dirac operator on the real line with non-Hermitian potential $V$ lie in the disjoint union of two disks in the right and left half plane, respectively, provided that the $L^1-norm$ of $V$ is bounded from above by the speed of light times the reduced Planck constant. An analogous result for the Schr\"odinger operator, originally proved by Abramov, Aslanyan and Davies, emerges in the nonrelativistic limit. For massless Dirac operators, the condition on $V$ implies the absence of nonreal eigenvalues. Our results are further generalized to potentials with slower decay at infinity. As an application, we determine bounds on resonances and embedded eigenvalues of Dirac operators with Hermitian dilation-analytic potentials

Short-time Gibbsianness for Infinite-dimensional Diffusions with Space-Time Interaction

We consider a class of infinite-dimensional diffusions where the interaction between the components is both spatial and temporal. We start the system from a Gibbs measure with finite-range uniformly bounded interaction. Under suitable conditions on the drift, we prove that there exists $t_0>0$ such that the distribution at time $t\leq t_0$ is a Gibbs measure with absolutely summable interaction. The main tool is a cluster expansion of both the initial interaction and certain time-reversed Girsanov factors coming from the dynamics

Optical Imaging and Spectroscopic Observation of the Galactic Supernova Remnant G85.9-0.6

Optical CCD imaging with H$\alpha$ and [SII] filters and spectroscopic observations of the galactic supernova remnant G85.9-0.6 have been performed for the first time. The CCD image data are taken with the 1.5m Russian-Turkish Telescope (RTT150) at TUBITAK National Observatory (TUG) and spectral data are taken with the Bok 2.3 m telescope on Kitt Peak, AZ. The images are taken with narrow-band interference filters H$\alpha$, [SII] and their continuum. [SII]/H$\alpha$ ratio image is performed. The ratio obtained from [SII]/H$\alpha$ is found to be $\sim$0.42, indicating that the remnant interacts with HII regions. G85.9-0.6 shows diffuse-shell morphology. [SII]$\lambda\lambda 6716/6731$ average flux ratio is calculated from the spectra, and the electron density $N_{e}$ is obtained to be 395 $cm^{-3}$. From [OIII]/H$\beta$ ratio, shock velocity has been estimated, pre-shock density of $n_{c}=14$ $cm^{-3}$, explosion energy of $E=9.2\times10^{50}$ ergs, interstellar extinction of $E(B-V)=0.28$, and neutral hydrogen column density of $N(HI)=1.53\times10^{21}$ $cm^{-2}$ are reported.Comment: 20 pages, 4 tables, 4 figures. Accepted for publication in Astrophysics & Space Scienc