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A fixed-target platform for serial femtosecond crystallography in a hydrated environment.
For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while maintaining low background remains a technical challenge for some experiments, especially where this methodology is applied to relatively low-ordered samples or those difficult to purify and crystallize in large quantities. This work demonstrates a scheme to encapsulate biological samples using polymer thin films and graphene to maintain sample hydration in vacuum conditions. The encapsulated sample is delivered into the X-ray beam on fixed targets for rapid scanning using the Roadrunner fixed-target system towards a long-term goal of low-background measurements on weakly diffracting samples. As a proof of principle, we used microcrystals of the 24 kDa rapid encystment protein (REP24) to provide a benchmark for polymer/graphene sandwich performance. The REP24 microcrystal unit cell obtained from our sandwiched in-vacuum sample was consistent with previously established unit-cell parameters and with those measured by us without encapsulation in humidified helium, indicating that the platform is robust against evaporative losses. While significant scattering from water was observed because of the sample-deposition method, the polymer/graphene sandwich itself was shown to contribute minimally to background scattering
The lower mass function of the young open cluster Blanco 1: from 30 Mjup to 3 Mo
We performed a deep wide field optical survey of the young (~100-150 Myr)
open cluster Blanco1 to study its low mass population well down into the brown
dwarf regime and estimate its mass function over the whole cluster mass
range.The survey covers 2.3 square degrees in the I and z-bands down to I ~ z ~
24 with the CFH12K camera. Considering two different cluster ages (100 and 150
Myr), we selected cluster member candidates on the basis of their location in
the (I,I-z) CMD relative to the isochrones, and estimated the contamination by
foreground late-type field dwarfs using statistical arguments, infrared
photometry and low-resolution optical spectroscopy. We find that our survey
should contain about 57% of the cluster members in the 0.03-0.6 Mo mass range,
including 30-40 brown dwarfs. The candidate's radial distribution presents
evidence that mass segregation has already occured in the cluster. We took it
into account to estimate the cluster mass function across the
stellar/substellar boundary. We find that, between 0.03Mo and 0.6Mo, the
cluster mass distribution does not depend much on its exact age, and is well
represented by a single power-law, with an index alpha=0.69 +/- 0.15. Over the
whole mass domain, from 0.03Mo to 3Mo, the mass function is better fitted by a
log-normal function with m0=0.36 +/- 0.07Mo and sigma=0.58 +/- 0.06. Comparison
between the Blanco1 mass function, other young open clusters' MF, and the
galactic disc MF suggests that the IMF, from the substellar domain to the
higher mass part, does not depend much on initial conditions. We discuss the
implications of this result on theories developed to date to explain the origin
of the mass distribution.Comment: 18 pages, 15 figures and 5 tables accepted in A&
Introducing a new breed of wine yeast: interspecific hybridisation between a commercial Saccharomyces cerevisiae wine yeast and Saccharomyces mikatae
Interspecific hybrids are commonplace in agriculture and horticulture; bread wheat and grapefruit are but two examples. The benefits derived from interspecific hybridisation include the potential of generating advantageous transgressive phenotypes. This paper describes the generation of a new breed of wine yeast by interspecific hybridisation between a commercial Saccharomyces cerevisiae wine yeast strain and Saccharomyces mikatae, a species hitherto not associated with industrial fermentation environs. While commercially available wine yeast strains provide consistent and reliable fermentations, wines produced using single inocula are thought to lack the sensory complexity and rounded palate structure obtained from spontaneous fermentations. In contrast, interspecific yeast hybrids have the potential to deliver increased complexity to wine sensory properties and alternative wine styles through the formation of novel, and wider ranging, yeast volatile fermentation metabolite profiles, whilst maintaining the robustness of the wine yeast parent. Screening of newly generated hybrids from a cross between a S. cerevisiae wine yeast and S. mikatae (closely-related but ecologically distant members of the Saccharomyces sensu stricto clade), has identified progeny with robust fermentation properties and winemaking potential. Chemical analysis showed that, relative to the S. cerevisiae wine yeast parent, hybrids produced wines with different concentrations of volatile metabolites that are known to contribute to wine flavour and aroma, including flavour compounds associated with non-Saccharomyces species. The new S. cerevisiae x S. mikatae hybrids have the potential to produce complex wines akin to products of spontaneous fermentation while giving winemakers the safeguard of an inoculated ferment.Jennifer R. Bellon, Frank Schmid, Dimitra L. Capone, Barbara L. Dunn, Paul J. Chamber
Kondo physics in carbon nanotubes
The connection of electrical leads to wire-like molecules is a logical step
in the development of molecular electronics, but also allows studies of
fundamental physics. For example, metallic carbon nanotubes are quantum wires
that have been found to act as one-dimensional quantum dots, Luttinger-liquids,
proximity-induced superconductors and ballistic and diffusive one-dimensional
metals. Here we report that electrically-contacted single-wall nanotubes can
serve as powerful probes of Kondo physics, demonstrating the universality of
the Kondo effect. Arising in the prototypical case from the interaction between
a localized impurity magnetic moment and delocalized electrons in a metallic
host, the Kondo effect has been used to explain enhanced low-temperature
scattering from magnetic impurities in metals, and also occurs in transport
through semiconductor quantum dots. The far higher tunability of dots (in our
case, nanotubes) compared with atomic impurities renders new classes of
Kondo-like effects accessible. Our nanotube devices differ from previous
systems in which Kondo effects have been observed, in that they are
one-dimensional quantum dots with three-dimensional metal (gold) reservoirs.
This allows us to observe Kondo resonances for very large electron number (N)
in the dot, and approaching the unitary limit (where the transmission reaches
its maximum possible value). Moreover, we detect a previously unobserved Kondo
effect, occurring for even values of N in a magnetic field.Comment: 7 pages, pdf onl
Identification of adults with symptoms suggestive of obstructive airways disease: Validation of a postal respiratory questionnaire
BACKGROUND: Two simples scoring systems for a self-completed postal respiratory questionnaire were developed to identify adults who may have obstructive airways disease. The objective of this study was to validate these scoring systems. METHOD: A two-stage design was used. All adults in two practice populations were sent the questionnaire and a stratified random sample of respondents was selected to undergo full clinical evaluation. Three respiratory physicians reviewed the results of each evaluation. A majority decision was reached as to whether the subject merited a trial of obstructive airways disease medication. This clinical decision was compared with two scoring systems based on the questionnaire in order to determine their positive predictive value, sensitivity and specificity. RESULTS: The PPV (positive predictive value) of the first scoring system was 75.1% (95% CI 68.6–82.3), whilst that of the second system was 82.3% (95% CI 75.9–89.2). The more stringent second system had the greater specificity, 97.1% (95% CI 96.0–98.2) versus 95.3% (95% CI 94.0–96.7), but poorer sensitivity 46.9% (95% CI 33.0–66.8) versus 50.3% (95% CI 35.3–71.6). CONCLUSION: This scoring system based on the number of symptoms/risk factors reported via a postal questionnaire could be used to identify adults who would benefit from a trial of treatment for obstructive airways disease
The Effect of Incremental Context on Conceptual Processing: Evidence from Visual World and Reading Experiments
Abstract The analysis of the internal structure of concepts reveals the presence of a substantial amount of contextual information. Even though this interaction is easily recognizable, it is not clear how contextual information is processed and included into concept representations. The aim of this paper is to shed light on this question by analyzing the effect that an increasing amount of context exerts on conceptual processing. We report a self-paced reading experiment and a visual world experiment to test two hypotheses about the integration of context information: the incremental activation hypothesis suggests that the degree of facilitation in concept processing increases with the amount of context available; and the immediate activation hypothesis states that once a sufficient amount of contextual support is reached, no more facilitation occurs. Our data are compatible with the latter account
Things change: Women’s and men’s marital disruption dynamics in Italy during a time of social transformations, 1970-2003
We study women’s and men’s marital disruption in Italy between 1970 and 2003. By applying an event-history analysis to the 2003 Italian variant of the Generations and Gender Survey we found that the spread of marital disruption started among middle-highly educated women. Then in recent years it appears that less educated women have also been able to dissolve their unhappy unions. Overall we can see the beginning of a reversed educational gradient from positive to negative. In contrast the trend in men’s marital disruption risk appears as a change over time common to all educational groups, although with persisting educational differentials.determinants, educational differences, event history analysis, gender difference, Italy, marital disruption
Tuning ultrafast electron thermalization pathways in a van der Waals heterostructure
Ultrafast electron thermalization - the process leading to Auger
recombination, carrier multiplication via impact ionization and hot carrier
luminescence - occurs when optically excited electrons in a material undergo
rapid electron-electron scattering to redistribute excess energy and reach
electronic thermal equilibrium. Due to extremely short time and length scales,
the measurement and manipulation of electron thermalization in nanoscale
devices remains challenging even with the most advanced ultrafast laser
techniques. Here, we overcome this challenge by leveraging the atomic thinness
of two-dimensional van der Waals (vdW) materials in order to introduce a highly
tunable electron transfer pathway that directly competes with electron
thermalization. We realize this scheme in a graphene-boron nitride-graphene
(G-BN-G) vdW heterostructure, through which optically excited carriers are
transported from one graphene layer to the other. By applying an interlayer
bias voltage or varying the excitation photon energy, interlayer carrier
transport can be controlled to occur faster or slower than the intralayer
scattering events, thus effectively tuning the electron thermalization pathways
in graphene. Our findings, which demonstrate a novel means to probe and
directly modulate electron energy transport in nanoscale materials, represent
an important step toward designing and implementing novel optoelectronic and
energy-harvesting devices with tailored microscopic properties.Comment: Accepted to Nature Physic
Ori-Finder: A web-based system for finding oriCs in unannotated bacterial genomes
<p>Abstract</p> <p>Background</p> <p>Chromosomal replication is the central event in the bacterial cell cycle. Identification of replication origins (<it>oriC</it>s) is necessary for almost all newly sequenced bacterial genomes. Given the increasing pace of genome sequencing, the current available software for predicting <it>oriC</it>s, however, still leaves much to be desired. Therefore, the increasing availability of genome sequences calls for improved software to identify <it>oriC</it>s in newly sequenced and unannotated bacterial genomes.</p> <p>Results</p> <p>We have developed Ori-Finder, an online system for finding <it>oriC</it>s in bacterial genomes based on an integrated method comprising the analysis of base composition asymmetry using the <it>Z</it>-curve method, distribution of DnaA boxes, and the occurrence of genes frequently close to <it>oriC</it>s. The program can also deal with unannotated genome sequences by integrating the gene-finding program ZCURVE 1.02. Output of the predicted results is exported to an HTML report, which offers convenient views on the results in both graphical and tabular formats.</p> <p>Conclusion</p> <p>A web-based system to predict replication origins of bacterial genomes has been presented here. Based on this system, <it>oriC </it>regions have been predicted for the bacterial genomes available in GenBank currently. It is hoped that Ori-Finder will become a useful tool for the identification and analysis of <it>oriC</it>s in both bacterial and archaeal genomes.</p
Transcription factor 7-like 2 (TCF7L2) variant is associated with familial breast cancer risk: a case-control study
BACKGROUND: The transcription factor 7-like 2 (TCF7L2) is a critical component of the Wnt/β-catenin pathway. Aberrant TCF7L2 expression modifies Wnt signaling and mediates oncogenic effects through the upregulation of c-MYC and cyclin D. Genetic alterations in TCF7L2 may therefore affect cancer risk. Recently, TCF7L2 variants, including the microsatellite marker DG10S478 and the nearly perfectly linked SNP rs12233372, were identified to associate with type 2 diabetes. METHODS: We investigated the effect of the TCF7L2 rs12255372 variant on familial breast cancer (BC) risk by means of TaqMan allelic discrimination, analyzing BRCA1/2 mutation-negative index patients of 592 German BC families and 735 control individuals. RESULTS: The T allele of rs12255372 showed an association with borderline significance (OR = 1.19, 95% C.I. = 1.01-1.42, P = 0.04), and the Cochran-Armitage test for trend revealed an allele dose-dependent association of rs12255372 with BC risk (P(trend )= 0.04). CONCLUSION: Our results suggest a possible influence of TCF7L2 rs12255372 on the risk of familial BC
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