Ecophenotypic plasticity leads to extraordinary gastropod shells found on the "Roof of the World"

The often extraordinary shell forms and shapes of gastropods found in palaeolakes, such as the highly diverse Gyraulus fauna of the famous Steinheim Basin, have been puzzling evolutionary biologists for centuries, and there is an ongoing debate whether these aberrant shell forms are indicative of true species (or subspecies) or ecophenotypic morphs. Interestingly, one of the Steinheim Gyraulus morphs – a corkscrew-like open-coiled shell – has a recent analogue in the Lake Bangong drainage system on the western Tibetan Plateau. Therefore, a combination of morphological, molecular, palaeolimnological, and ecological analyses was used in this study to assess whether the extraordinary shell shape in Gyraulus sp. from this drainage system represents a (young) ecophenotypic phenomenon or if it has been genetically fixed over an extended period of time. Our morphological, ecological, and palaeolimnological data suggest that the corkscrew-like specimens remain restricted to a small pond near Lake Bangong with an elevated pH value and that the colonization may have occurred recently. The phylogenetic reconstruction based on two gene fragments shows that these nonplanispiral specimens cluster within the previous described Tibetan Plateau Gyraulus clade N2. A network analysis indicates that some haplotypes are even shared by planispiral and nonplanispiral specimens. Given the ephemerality of the phenomenon, the compact network patterns inferred, the likely young phylogenetic age of the aberrant Gyraulus shells studied, and the ecological peculiarities of the study site, we suggest that the evolution of the aberrant shell forms on the Tibetan Plateau could likely be considered as a rapid ecophenotypic response, possibly induced by ecological stress. This finding may thus have implications for the ongoing debate about the processes that have caused the extraordinary shell diversity in palaeolakes such as the Steinheim Basin

Allocation in LCA of wood-based products experiences of cost action E9: Part II. Examples

Goal and Background: The treatment of allocation in the descriptive LCA of wood-based products has been discussed for a long time and different solutions have been presented. In general, it is accepted that the influence of different allocation procedures on the results of LCA of wood-based products can be very significant. This paper is a result of the Cost Action E9 ‘Life cycle assessment of forestry and forest products' and represents the experience of involved Cost E9 delegates. Objective: Wood is a renewable material that can be used for wood products and energy production. Consistent methodological procedures are needed in order to correctly address the twofold nature of wood as a material and fuel, the multi-functional wood processing generating large quantities of co-products, and reuse or recycling of paper and wood. Ten different processes in LCAs of wood-based products are identified, where allocation questions can occur: forestry, sawmill, wood industry, pulp and paper industry, particle board industry, recycling of paper, recycling of wood-based boards, recycling of waste wood, combined heat and power production, landfill. Methodology: Following ISO 14 041 a step-wise procedure for system boundary setting and allocation are outlined. As a first priority allocation should be avoided by system expansion, thus adding additional functions to the functional unit. Alternatively, the avoided-burden approach can be followed by subtracting substituted functions of wood that are additionally provided. If allocation cannot be avoided, some allocations methods from case studies are described. Conclusions: The following conclusions for allocation in LCA of wood-based products are given. 1) Avoid allocation by expansion of system boundaries by combining material and energy aspects of wood, meaning a combination of LCA of wood products and of energy from wood with a functional unit for products and energy. 2) Substitute energy from wood with conventional energy in the LCA of wood products to get the functional unit of the wood product only, but identify the criteria for the substituted energy. 3) Substitution of wooden products with non-wooden products in LCA of bioenergy is not advisable, because the substitution criteria can be too complex. 4) If avoiding allocation is not possible, the reasons should be documented. 5) Different allocation procedures must be analysed and documented. In many cases, it seems necessary to make a sensitivity analysis of different allocation options for different environmental effects. It can also be useful to get the acceptance of the chosen allocation procedure by external experts. 6) Different allocation factors, e.g. mass or economic value, are allowed within the same LCA. 7) For allocation of forestry processes it is necessary to describe the main function of the forest where the raw material is taken out. In some cases different types or functions of forests must be considered and described. 8) Regarding the experiences from the examples, the following most practical allocation for some specific processes are identified: forestry: mass or volume; sawmill: mass or volume and proceeds; wood industry: mass and proceed

Allocation in lca of wood-based products experiences of cost action E9 part i. methodology

Goal and Background: The treatment of allocation in the descriptive LCA of wood-based products has been discussed for a long time and different solutions have been presented. In general, it is accepted that the influence of different allocation procedures on the results of LCA of wood-based products can be very significant. This paper is a result of the Cost Action E9 'Life cycle assessment of forestry and forest products' and represents the experience of involved Cost E9 delegates. Objective: Wood is a renewable material that can be used for wood products and energy production. Consistent methodological procedures are needed in order to correctly address the twofold nature of wood as a material and fuel, the multi-functional wood processing generating large quantities of co-products, and reuse or recycling of paper and wood. Ten different processes in LCAs of wood-based products are identified, where allocation questions can occur: forestry, sawmill, wood industry, pulp and paper industry, particle board industry, recycling of paper, recycling of wood-based boards, recycling of waste wood, combined heat and power production, landfill. Methodology: Following ISO 14 041 a step-wise procedure for system boundary setting and allocation are outlined. As a first priority allocation should be avoided by system expansion, thus adding additional functions to the functional unit. Alternatively, the avoided-burden approach can be followed by subtracting substituted functions of wood that are additionally provided. If allocation cannot be avoided, some allocations methods from case studies are described. Conclusions: The following conclusions for allocation in LCA of wood-based products are given. 1) Avoid allocation by expansion of system boundaries by combining material and energy aspects of wood, meaning a combination of LCA of wood products and of energy from wood with a functional unit for products and energy. 2) Substitute energy from wood with conventional energy in the LCA of wood products to get the func-tional unit of the wood product only, but identify the criteria for the substituted energy. 3) Substitution of wooden products with non-wooden products in LCA of bioenergy is not advis able, because the substitution criteria can be too complex. 4) If avoiding allocation is not possible, the reasons should be documented. 5) Different allocation procedures must be analysed and documented. In many cases, it seems necessary to make a sensitivity analysis of different allocation options for different environmental effects. It can also be useful to get the acceptance of the chosen allocation procedure by external experts. 6) Different allocation factors, e.g. mass or economic value, are allowed within the same LCA. 7) For allocation of forestry processes it is necessary to describe the main function of the forest where the raw material is taken out. In some cases different types or functions of forests must be considered and described. 8) Regarding the experiences from the examples, the following most practical allocation for some specific processes are identified: forestry: mass or volume; sawmill: mass or volume and proceeds; wood industry: mass and proceed

5-Bromo-17-nitro-26,28-prop-2-en­oxy-25,27-dipropoxycalix[4]arene

Mol­ecules of the title compound, C40H42BrNO6, are located on a crystallographic twofold rotation axis. As a result, the nitro group and bromine residue are mutually disordered with equal occupancies. The prop­oxy-substituted aromatic rings are close to parallel to each other [dihedral angle = 21.24 (1)°], whereas the propen­oxy-substituted rings enclose a dihedral angle of 70.44 (1)°. The dihedral angles between the methyl­ene C atoms and the aromatic rings shows that the propen­oxy substituted rings are bent away from the calixarene cavity [dihedral angle between the planes = 35.22 (8)°], whereas the prop­oxy-substituted rings are almost perpendicular [79.38 (10)°] to the plane of the methyl­ene C atoms

Relevance of different prior knowledge sources for inferring gene interaction networks

When inferring networks from high-throughput genomic data, one of the main challenges is the subsequent validation of these networks. In the best case scenario, the true network is partially known from previous research results published in structured databases or research articles. Traditionally, inferred networks are validated against these known interactions. Whenever the recovery rate is gauged to be high enough, subsequent high scoring but unknown inferred interactions are deemed good candidates for further experimental validation. Therefore such validation framework strongly depends on the quantity and quality of published interactions and presents serious pitfalls: (1) availability of these known interactions for the studied problem might be sparse; (2) quantitatively comparing different inference algorithms is not trivial; and (3) the use of these known interactions for validation prevents their integration in the inference procedure. The latter is particularly relevant as it has recently been showed that integration of priors during network inference significantly improves the quality of inferred networks. To overcome these problems when validating inferred networks, we recently proposed a data-driven validation framework based on single gene knock-down experiments. Using this framework, we were able to demonstrate the benefits of integrating prior knowledge and expression data. In this paper we used this framework to assess the quality of different sources of prior knowledge on their own and in combination with different genomic data sets in colorectal cancer. We observed that most prior sources lead to significant F-scores. Furthermore, their integration with genomic data leads to a significant increase in F-scores, especially for priors extracted from full text PubMed articles, known co-expression modules and genetic interactions. Lastly, we observed that the results are consistent for three different data sets: experimental knock-down data and two human tumor data sets

The Effects of Immobility on Long Bone Remodelling in the Rhesus Monkey

Using Frost\u27s method for undecalcified bone sections, long bones of the lower extremities of ten rhesus monkeys were examined following two months\u27 immobilization and compared with thirteen controls. A decrease in appositional rate and in the surface extent of the ossification process were noted in the immobilized animals. No typical change in resorption was noted. The immobilized animals showed a decreased cortical-total area ratio. These findings suggest that a decrease in activity affects bone by depressing functions mediated by the osteoblast without necessarily evoking an Increased remodelling response

Effects of spermidine supplementation on cognition and biomarkers in older adults with subjective cognitive decline (SmartAge)—study protocol for a randomized controlled trial

Background: Given the global increase in the aging population and age-related diseases, the promotion of healthy aging is one of the most crucial public health issues. This trial aims to contribute to the establishment of effective approaches to promote cognitive and brain health in older individuals with subjective cognitive decline (SCD). Presence of SCD is known to increase the risk of objective cognitive decline and progression to dementia due to Alzheimer’s disease. Therefore, it is our primary goal to determine whether spermidine supplementation has a positive impact on memory performance in this at-risk group, as compared with placebo. The secondary goal is to examine the effects of spermidine intake on other neuropsychological, behavioral, and physiological parameters. Methods: The SmartAge trial is a monocentric, randomized, double-blind, placebo-controlled phase IIb trial. The study will investigate 12 months of intervention with spermidine-based nutritional supplementation (target intervention) compared with 12months of placebo intake (control intervention). We plan to recruit 100 cognitively normal older individuals with SCD from memory clinics, neurologists and general practitioners in private practice, and the general population. Participants will be allocated to one of the two study arms using blockwise randomization stratified by age and sex with a 1:1 allocation ratio. The primary outcome is the change in memory performance between baseline and post-intervention visits (12 months after baseline). Secondary outcomes include the change in memory performance from baseline to follow-up assessment (18months after baseline), as well as changes in neurocognitive, behavioral, and physiological parameters (including blood and neuroimaging biomarkers), assessed at baseline and post-intervention. Discussion: The SmartAge trial aims to provide evidence of the impact of spermidine supplementation on memory performance in older individuals with SCD. In addition, we will identify possible neurophysiological mechanisms of action underlying the anticipated cognitive benefits. Overall, this trial will contribute to the establishment of nutrition intervention in the prevention of Alzheimer’s disease