Development and Validation of a Specific Stability Indicating High Performance Liquid Chromatographic Method for Valsartan

A stability-indicating HPLC assay method has been developed and validated for valsartan in bulk drug and pharmaceutical dosage forms. An isocratic RP-HPLC was achieved on Waters 2695 using Symmetry C18 (250mm × 4.6mm × 5μ) column with the mobile phase consisting of 0.02 mM sodium dihydrogen ortho-phosphate, pH adjusted to 2.5 using ortho-phosphoric acid (solvent A), and acetonitrile (solvent B) in the ratio of 58:42 %v/v. The stress testing of valsartan was carried out under acidic, alkaline, oxidative, thermal, and photolytic conditions. Valsartan was well resolved from its degradation products. The proposed method was validated as per ICH guidelines. The method was found to be suitable for the quality control of valsartan in bulk and pharmaceutical dosage forms as well as the stability-indicating studies

Chiral Four-Wave-Mixing signals with circularly-polarized X-ray pulses

Chiral four-wave-mixing signals are calculated using the irreducible tensor formalism. Different polarization and crossing angle configurations allow to single out the magnetic dipole and the electric quadrupole interactions. Other configurations can reveal that the chiral interaction occurs at a given step within the nonlinear interaction pathways contributing to the signal. Applications are made to the study of valence excitations of S-ibuprofen by chiral Stimulated X-ray Raman signals at the Carbon K-edge and by chiral visible 2D Electronic Spectroscopy.teraction pathways contributing to the signal.Comment: 33 pages, 10 figure

Treatment outcome in infant acute lymphoblastic leukemia

peer reviewe

Qualitative organic micro-analysis based on physical constants: Identification of amino-acids

SCOPUS: le.jinfo:eu-repo/semantics/publishe

Microanalyse fonctionnelle organique basée sur la détermination des constantes physiques. Identification des acides aminés

This study, though entitled as a contribution to the microdetermination of the physical constants of organic compounds, is at the same time a contribution to functional organic microanalysis, in the sense that it involves the use of two physical constants: the eutectic fusion temperature and the index of refraction for identifying amino acids. All the determinations can be made in one minute and on only one crystal. This method may be extended to other classes of compounds and is capable of rendering great service in industrial and biochemical control. The method given here for the identification of organic compounds competes advantageously with that of preparing derivatives of organic materials and furthermore it permits speeding up the deductions of classical qualitative organic analysis based on the differences in solubility (method of Staudinger, Kamm, Shriner, etc.). Quantitative developments are being studied. © 1953 Springer-Verlag.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Clinical Case of the Month. Osteoporosis and Beta-Thalassemia

peer reviewe

Primary intraventricular brain abscess.

We report a primary intraventricular brain abscess in a 13-year-old boy. We discuss possible explanations for this rare occurrence

Contribution of NMR spectroscopy to the differential diagnosis of a recurrent cranial mass 7 years after irradiation for a pediatric ependymoma.

CASE REPORT: We describe the case of a 5-year-old-boy who underwent surgery and focal radiotherapy for an anaplastic ependymoma of the fourth ventricle. One year later, a spinal metastasis was treated the same way. Six years later, a 16-mm lesion was found on a control MRI in the posterior fossa. To help the differential diagnosis between a relapse, a radio-induced modification, and a new tumor, magnetic resonance spectroscopy was performed. The main findings were a peak at the expected resonance frequency of reduced glutathione, a prominent peak of glutamate/glutamine compounds, a low N-acetylaspartate, and the absence of elevated choline. These findings were suggestive of a meningioma, although the latency between irradiation and development of the lesion was quite short. The diagnosis was confirmed by the pathological examination. CONCLUSION: This case exemplifies the fact that magnetic resonance spectroscopy provides useful biochemical information in such a clinical setting.Case ReportsJournal ArticleSCOPUS: ar.jinfo:eu-repo/semantics/publishe

Paediatric and adolescent alveolar soft part sarcoma: A joint series from European cooperative groups

Abstract BackgroundAlveolar soft part sarcomas (ASPS) are generally chemo- and radio-resistant mesenchymal tumours, with no standardized treatment guidelines. We describe the clinical behaviour of paediatric ASPS and compare these features to previously reported adult series. Patients and MethodsThe clinical data of 51 children and adolescents with ASPS, prospectively enrolled in or treated according to seven European Paediatric trials were analysed. ResultsMedian age was 13 years [range: 2-21]. Primary sites included mostly limbs (63%). IRS post-surgical staging was: IRS-I (complete resection) 35%, II (microscopic residual disease) 20%, III (gross residual disease) 18% and IV (metastases) 27%. Only 3 of the 18 evaluable patients (17%) obtained a response to conventional chemotherapy. After a median follow-up of 126 months (range: 9-240), 14/18 patients with IRS-I tumour, 10/10 IRS-II, 7/9 IRS-III and 2/14 IRS-IV were alive in remission. Sunitinib treatment achieved two very good partial responses in four patients. Ten-year overall survival (OS) and event free survival (EFS) was 78.07% and 62.8 +/- 7% respectively. Stage IV, size >5cm and T2 tumours had a poorer outcome, but only IRS staging was an independent prognostic factor. ConclusionsASPS is a very rare tumour frequently arising in adolescents and in the extremities, and chemo resistant. Local surgical control is critical. ASPS is a poorly chemo sensitive tumour. For IRS-III/IV tumours, delayed radical local therapies including surgery are essential. Metastatic patients had a poor prognosis but targeted therapies showed promising results. Pediatr Blood Cancer 2013;60:1826-1832. (c) 2013 Wiley Periodicals, Inc