Human pregnane X receptor is expressed in breast carcinomas, potential heterodimers formation between hPXR and RXR-alpha.

The human pregnane X receptor (hPXR) is an orphan nuclear receptor that induces transcription of response elements present in steroid-inducible cytochrome P 450 gene promoters. This activation requires the participation of retinoid X receptors (RXRs), needed partners of hPXR to form heterodimers. We have investigated the expression of hPXR and RXRs in normal, premalignant, and malignant breast tissues, in order to det. whether their expression profile in localized infiltrative breast cancer is assocd. with an increased risk of recurrent disease. Methods: Breast samples from 99 patients including benign breast diseases, in situ and infiltrative carcinomas were processed for immunohistochem. and Western-blot anal. Results: Cancer cells from patients that developed recurrent disease showed a high cytoplasmic location of both hPXR isoforms. Only the infiltrative carcinomas that relapsed before 48 mo showed nuclear location of hPXR isoform 2. This location was assocd. with the nuclear immunoexpression of RXR-alpha. Conclusion: Breast cancer cells can express both variants 1 and 2 of hPXR. Infiltrative carcinomas that recurred showed a nuclear location of both hPXR and RXR-alpha; therefore, the overexpression and the subcellular location changes of hPXR could be considered as a potential new prognostic indicator

Synthesis of axially chiral vinylallenes, and their subsequent reactions : silver(I) catalyzed enantioselective diels-alder reaction and aerobic oxidation

abstractChemistryMasterMaster of Philosoph

Revista de enseñanza universitaria

Resumen tomado del autor. Resumen también en inglésDescribe un proyecto de innovación consistente en el diseño de un material didáctico hipermedia (distribuido en CD-ROM) que permite el auto-aprendizaje de un programa de facturación. Con ello tratamos de dar solución a dos problemas que venimos encontrándonos a la hora de impartir su docencia: a) escasez de tiempo para impartirlo (un programa informático relativamente complejo) y b) la falta de flexibilidad que caracteriza habitualmente a los manuales de ayuda de este tipo de programas. Este proyecto se incluye, además, en otro de mayor alcance en el que tratamos de evaluar la eficacia y eficiencia de la metodología utilizada.AndalucíaUniversidad de León. Biblioteca General San Isidoro. Hemeroteca; Campus de Vegazana, s. n.; 24071 León; Tel. +34987291685; [email protected]

Revista de enseñanza universitaria

Resumen basado en el del autorLas percepciones de los alumnos son una valiosa fuente de información para la mejora y rediseño del software educativo y su posterior introducción en las asignaturas universitarias. Describe el proceso de evaluación seguido con una herramienta hipermedia destinada al estudio de un programa de facturación, analizando sus resultados e implicaciones en la futuras fases de este proyecto de innovación docente.AndalucíaUniversidad de León. Facultad de Educación. Servicio de Biblioteca; Campus de Vegazana, s. n.; 24071 León; Tel. +34987291146; Fax +34987291145; [email protected]; [email protected]

The Santa Pola saltern as a model for studying the microbiota of hypersaline environments

Multi-pond salterns constitute an excellent model for the study of the microbial diversity and ecology of hypersaline environments, showing a wide range of salt concentrations, from seawater to salt saturation. Accumulated studies on the Santa Pola (Alicante, Spain) multi-pond solar saltern during the last 35 years include culture-dependent and culture-independent molecular methods and metagenomics more recently. These approaches have permitted to determine in depth the microbial diversity of the ponds with intermediate salinities (from 10 % salts) up to salt saturation, with haloarchaea and bacteria as the two main dominant groups. In this review, we describe the main results obtained using the different methodologies, the most relevant contributions for understanding the ecology of these extreme environments and the future perspectives for such studies.Ministerio de Ciencia e Innovación CGL2013-46941-P, CGL2010-19303, CGL2009-12651-C02-01, BIO2011-12879-E, BIO2008-02444, CDS2009-00006National Science Foundation DEB-0919290European Commission 311975Generalitat Valenciana DIMEGEN PROMETEO/2010/089, ACOMP/2009/155Junta de Andalucía P10-CVI-622