Adaptive Image Contrast Enhancement by Computing Distances into a 4-Dimensional Fuzzy Unit Hypercube

A new fuzzy procedure for adaptive gray-level image contrast enhancement (CE) is presented in this paper. Starting from the pixels belonging to a normalized gray-level image, an appropriate smooth S-shaped fuzzy membership function (MF) is considered for gray-scale transformation and is adaptively developed through noise reduction and information loss minimization. Then, a set of fuzzy patches is extracted from the MF, and for each support of each patch, we compute four ascending-order statistics that become points inside a 4-D fuzzy unit hypercube after a suitable fuzzification step. CE is performed by computing the distances among the above points and the points of maximum darkness and maximum brightness (special vertexes in the hypercube), and by determining the rotation of the tangent line to the MF around a crucial point where fuzzy patches and the MF coexist. The proposed procedure enables high CE in all the treated images with performance that is fully comparable with that obtained by three more sophisticated fuzzy techniques and by standard histogram equalization

Joint use of eddy current imaging and fuzzy similarities to assess the integrity of steel plates

AbstractSteel plates bi-axially loaded are characterized by mechanical deformations whose 2D image representations are very difficult to achieve. In this work, the authors propose an innovative approach based on eddy current techniques for obtaining 2D electrical maps to assess the mechanical integrity of a steel plate. The procedure, also exploiting fuzzy similarity computations, translates the problem of the assessment of the mechanical integrity of a steel plate into a suitable classification problem. The results obtained by this proposed procedure show performances comparable to those provided by well-established soft computing approaches with a higher computational complexity

Notulae to the Italian native vascular flora: 1

In this contribution, new data concerning the Italian distribution of native vascular flora are presented. It includes new records, exclusions, and confirmations pertaining to the Italian administrative regions for taxa in the genera Arundo, Bromopsis, Cistus, Crocus, Festuca, Galeopsis, Genista, Lamium, Leucanthemum, Nerium, Orobanche, Peucedanum, Pilosella, Polycnemum, Stipa and Viola

A Semi-Linear Elliptic Model for a Circular Membrane MEMS Device Considering the Effect of the Fringing Field

In this study, an accurate analytic semi-linear elliptic differential model for a circular membrane MEMS device, which considers the effect of the fringing field on the membrane curvature recovering, is presented. A novel algebraic condition, related to the membrane electromechanical properties, able to govern the uniqueness of the solution, is also demonstrated. Numerical results for the membrane profile, obtained by using the Shooting techniques, the Keller–Box scheme, and the III/IV Stage Lobatto IIIa formulas, have been carried out, and their performances have been compared. The convergence conditions, and the possible presence of ghost solutions, have been evaluated and discussed. Finally, a practical criterion for choosing the membrane material as a function of the MEMS specific application is presented

Joint use of eddy current imaging and fuzzy similarities to assess the integrity of steel plates

Steel plates bi-axially loaded are characterized by mechanical deformations whose 2D image representations are very difficult to achieve. In this work, the authors propose an innovative approach based on eddy current techniques for obtaining 2D electrical maps to assess the mechanical integrity of a steel plate. The procedure, also exploiting fuzzy similarity computations, translates the problem of the assessment of the mechanical integrity of a steel plate into a suitable classification problem. The results obtained by this proposed procedure show performances comparable to those provided by well-established soft computing approaches with a higher computational complexity

A PRELIMINARY COMPARATIVE STUDY ON THE EFFECTS OF PILATES TRAINING ON PHYSICAL FITNESS OF YOUNG FEMALE VOLLEYBALL PLAYERS

Pilates Trainin

Leishmania infantum trypanothione reductase is a promiscuous enzyme carrying an nadph:o2 oxidoreductase activity shared by glutathione reductase

Abstract BACKGROUND: Leishmania infantum is a protozoan of the trypanosomatid family causing visceral leishmaniasis. Leishmania parasites are transmitted by the bite of phlebotomine sand flies to the human host and are phagocyted by macrophages. The parasites synthesize N1-N8-bis(glutationyl)-spermidine (trypanothione, TS2), which furnishes electrons to the tryparedoxin-tryparedoxin peroxidase couple to reduce the reactive oxygen species produced by macrophages. Trypanothione is kept reduced by trypanothione reductase (TR), a FAD-containing enzyme essential for parasite survival. METHODS: The enzymatic activity has been studied by stopped-flow, absorption spectroscopy, and amperometric measurements. RESULTS: The study reported here demonstrates that the steady-state parameters change as a function of the order of substrates addition to the TR-containing solution. In particular, when the reaction is carried out by adding NADPH to a solution containing the enzyme and trypanothione, the KM for NADPH decreases six times compared to the value obtained by adding TS2 as last reagent to start the reaction (1.9 vs. 12μM). More importantly, we demonstrate that TR is able to catalyze the oxidation of NADPH also in the absence of trypanothione. Thus, TR catalyzes the reduction of O2 to water through the sequential formation of C(4a)-(hydro)peroxyflavin and sulfenic acid intermediates. This NADPH:O2 oxidoreductase activity is shared by Saccharomyces cerevisiae glutathione reductase (GR). CONCLUSIONS: TR and GR, in the absence of their physiological substrates, may catalyze the electron transfer reaction from NADPH to molecular oxygen to yield water. GENERAL SIGNIFICANCE: TR and GR are promiscuous enzymes

Bari Shoulder Telemedicine Examination Protocol (B-STEP): A Standard Protocol for Personalized Remote Shoulder Examination

The COVID-19 pandemic drastically changed many aspects of the traditional functioning of health systems all around the world. In Italy, as reported by the CIO, compared to the previous year, there was a significant reduction in 2020 in overall outpatient activities by up to 75%. These data support the need for telemedicine, which represents a current challenge and can no longer be postponed in the future. This study aims to elaborate on a possible model for remote shoulder examination based on traditional tests to improve the quality of telemedicine in orthopedic and rehabilitation. Between May 2020 and November 2020, ten orthopedic surgeons individually examined six patients with a known shoulder disorder, both in hospital and via webcam according to the previously shared protocol (B-STEP). According to the 10 observers, completing 100% of the ASES score and at least 87.5% of the Constant score is possible. Shoulder ROM and many specific tests are also reproducible via webcam, but with less sensitivity, according to the subjective opinion of observers. The B-STEP is a useful protocol for the standardization of the objective examination of the shoulder via webcam. Further studies are necessary to determine if the B-STEP protocol is useful for diagnosing pathology in unknown patients and evaluating its sensitivity and specificity for each pathology

Anterior Cruciate Ligament Reconstruction with Quadrupled Semitendinosus Graft or Synthetic Ligament: Knee Stability and Clinical Outcomes at Three Years Follow-Up

'e incidence of anterior cruciate ligament reconstruction (ACLR) surgeries is increasing and graft choice is important for a rapid return to activity, especially in patients older than 30 years. 'e aim of this study is to compare in term of quality of life and knee stability of patients who undergone ACLR using quadrupled semitendinosus (ST4) graft against patients who undergone ACLR with synthetic ligaments. 'irty-nine patients undergoing ACLR were enrolled in the study and were divided into two groups: ACLR with synthetic ligaments-LARS (group A) and ACLR with quadrupled semitendinosus graft ST4 (group B). 'ey underwent surgery at Policlinico di Bari Orthopedic Unit between January 2017 and January 2020. Group A was composed by nineteen patients (36.16 ± 4.41 mean age-years, 22.47 ± 2.63 mean BMI-kg/m2, 39.37 ± 10.05 mean time evaluation after surgerymonths) and group B was composed by twenty patients (34.95 ± 3.59 mean age-years, 21.1 ± 2.88 mean BMI-kg/m2, 36.75 ± 8.69 mean time evaluation after surgery-months). For each patient, the following data were recorded: age; side of injury, BMI, date of surgery, anterior knee laxity with the arthrometer, and Lysholm knee scoring scale. Mean value of anterior tibial translation (ATT) in group A was 3.09mm± 0.65 and in group B was 2.66mm± 1.61 (pvalue of 0.1139). Mann–-Whitney U test used to compare the Lysholm means values between groups showed a pvalue of 0.9307. LARS has comparable clinical and functional outcomes compared with hamstring autografts at short-term of 3 years follow-up. Level of Evidence: IV

Structure-based discovery of the first non-covalent inhibitors of Leishmania major tryparedoxin peroxidase by high throughput docking

Leishmaniasis is a neglected vector-born disease caused by a protozoan of the genus Leishmania and affecting more than 1.300.000 people worldwide. The couple tryparedoxin/tryparedoxin peroxidase is essential for parasite survival in the host since it neutralizes the hydrogen peroxide produced by macrophages during the infection. Herein we report a study aimed at discovering the first class of compounds able to non-covalently inhibit tryparedoxin peroxidase. We have solved the high-resolution structure of Tryparedoxin peroxidase I from Leishmania major (LmTXNPx) in the reduced state and in fully folded conformation. A first series of compounds able to inhibit LmTXNPx was identified by means of the high throughput docking technique. The inhibitory activity of these compounds was validated by a Horseradish peroxidase-based enzymatic assay and their affinity for LmTXNPx calculated by surface plasmon resonance experiments. On the basis of these results, the analysis of the enzyme-inhibitor docked models allowed us to rationally design and synthesize a series of N,N-disubstituted 3-aminomethyl quinolones. These compounds showed an inhibitory potency against LmTXNPx in the micromolar range. Among them, compound 12 represents the first non-covalent LmTXNPx inhibitor reported to date and could pave the way to the discovery of a new class of drugs against leishmaniasis