Secondary structure of rhBMP-2 in a protective biopolymeric carrier material

Efficient delivery of growth factors is one of the great challenges of tissue engineering. Polyelectrolyte multilayer films (PEM) made of biopolymers have recently emerged as an interesting carrier for delivering recombinant human bone morphogenetic protein 2 (rhBMP-2 noted here BMP-2) to cells in a matrix-bound manner. We recently showed that PEM made of poly(l-lysine) and hyaluronan (PLL/HA) can retain high and tunable quantities of BMP-2 and can deliver it to cells to induce their differentiation in osteoblasts. Here, we investigate quantitatively by Fourier transform infrared spectroscopy (FTIR) the secondary structure of BMP-2 in solution as well as trapped in a biopolymeric thin film. We reveal that the major structural elements of BMP-2 in solution are intramolecular β-sheets and unordered structures as well as α-helices. Furthermore, we studied the secondary structure of rhBMP-2 trapped in hydrated films and in dry films since drying is an important step for future applications of these bioactive films onto orthopedic biomaterials. We demonstrate that the structural elements were preserved when BMP-2 was trapped in the biopolymeric film in hydrated conditions and, to a lesser extent, in dry state. Importantly, its bioactivity was maintained after drying of the film. Our results appear highly promising for future applications of these films as coatings of biomedical materials, to deliver bioactive proteins while preserving their bioactivity upon storage in dry state.This work was supported by the French Ministry of Research through an ANR-EmergenceBIO grant (ANR-09-EBIO-012-01), by the European Commission (FP7 program) via a European Research Council starting grant (BIOMIM, GA 259370), and by GRAVIT (081012_FIBIOS). C.P. is grafetul to IUF for financial support

Etude des effets des Bone Morphogenic Proteins sur la phase de consolidation osseuse dans un modèle d'ostéogénèse en distraction

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Le Chondroblastome de l'enfant (à propos d'une série multicentrique de 76 cas)

LYON1-BU Santé (693882101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Sauvegarde du patrimoine en cas de sinistre - Conception d'une solution de localisation et de surveillance à base de RFIDs actifs, défis et perspectives

International audienceFire as a main vector of collection destruction, remains a major concern in museums. In this paper we present an RFID-assisted localisation system which is intended for providing reliable tracking of both art-craft and fire fighters in a museum. Our project lies in embedding critical art craft information in specific on-site RFID tags so as to facilitate the localisation of that art craft, while minimizing dependence on the commandcenter. As a first step upon that goal, we present the challenges, the research perspectives and the design (as a work in progress) of such a system.La protection contre les incendies constitue l’une des pierres angulaires de la politique de sauvegarde du patrimoine menée par les musées et monuments historiques. Face à ce besoin, nous établissons les défis à relever, nos perspectives de recherche et les prémisses d’un système de surveillance d’une intervention suite à un incendie dans un musée. En munissant les oeuvres d’art d’étiquettes RFID et les combattants du feu de lecteurs RFID, notre objectif est de localiser dynamiquement les œuvres devant être mises à l’abri

Sauvegarde du patrimoine en cas de sinistre - Conception d'une solution de localisation et de surveillance à base de RFIDs actifs, défis et perspectives

International audienceFire as a main vector of collection destruction, remains a major concern in museums. In this paper we present an RFID-assisted localisation system which is intended for providing reliable tracking of both art-craft and fire fighters in a museum. Our project lies in embedding critical art craft information in specific on-site RFID tags so as to facilitate the localisation of that art craft, while minimizing dependence on the commandcenter. As a first step upon that goal, we present the challenges, the research perspectives and the design (as a work in progress) of such a system.La protection contre les incendies constitue l’une des pierres angulaires de la politique de sauvegarde du patrimoine menée par les musées et monuments historiques. Face à ce besoin, nous établissons les défis à relever, nos perspectives de recherche et les prémisses d’un système de surveillance d’une intervention suite à un incendie dans un musée. En munissant les oeuvres d’art d’étiquettes RFID et les combattants du feu de lecteurs RFID, notre objectif est de localiser dynamiquement les œuvres devant être mises à l’abri

Hereditary multiple exostoses of the ribs as an uncommon cause of pneumothorax

International audienceRationale: Hereditary multiple exostoses (HME) is a genetic musculoskeletal condition causing multiple exostoses. Rib location of exostosis can be complicated by thoracic injuries.Patient concerns and diagnoses: We report a case of pneumothorax in a 32-year-old man with a partial left-sided pneumothorax caused by an exostosis of the fourth and fifth ribs.Interventions and outcomes: Clinical and radiological presentations allowed a conservative management. A video-assisted thoracoscopic surgery was performed a few weeks later to avoid any recurrence.Lessons: Rib exostosis represents an unusual cause of pneumothorax. Any local modification of symptoms or size of the exostosis should lead to investigations in regard to chondrosarcoma transformation

Influence of preoperative biological parameters on postoperative complications and survival in spinal bone metastasis. A multicenter prospective study

International audienceIntroduction: Onset of spinal bone metastasis is a turning point in the progression of tumoral disease; although incidence is increasing, management is not standardized. Various prognostic scores are available, but advances in medical and surgical treatment have made them less well adapted, and sometimes discordant for a given patient. It would therefore be useful to develop new prognostic instruments. The aim of the present study was to identify biologic risk factors for onset of postoperative complications and death following spinal bone metastasis surgery.Material and methods: A prospective multicenter study included all patients operated on for spinal bone metastasis between November 2015 and May 2017. The main epidemiologic data and biologic data (CRP, albuminemia, calcemia) were collected preoperatively. Surgical strategy, death and/or postoperative complications were collected prospectively.Results: Five of the initial 264 patients died during the immediate postoperative course, and 107 within 6 months. At 1 year, 57 patients remained alive. Twenty-six (10%) were lost to follow-up. Preoperative albuminemia 2.6 nmol/L (8%) and CRP > 10 mg/L (47.5%) were associated with significantly elevated mortality. Only CRP elevation correlated with postoperative complications rate.Conclusion: The study confirmed the prognostic value of 3 biologic parameters (CRP level, albuminemia, calcemia) for survival after spinal bone metastasis surgery. A hybrid score taking account of not only clinical but also biologic parameters should be developed to improve estimation of survival

A decision-making tool to prescribe knee orthoses in daily practice for patients with osteoarthritis

International audienceObjective: To develop a decision-making tool (DMT) to facilitate the prescription of knee orthoses for patients with osteoarthritis (OA) in daily practice. Methods: A steering committee gathered a multidisciplinary task force experienced in OA management/clinical research. Two members performed a literature review with qualitative analysis of the highest-quality randomized controlled trials and practice guidelines to confirm evidence concerning knee orthosis for OA. A first DMT draft was presented to the task force in a 1-day meeting in January 2016. The first version of the DMT was criticized and discussed regarding everyday practice issues. Every step was discussed and amended until consensus agreement was achieved within the task force. Then 4 successive consultation rounds occurred by electronic communication, first withprimary-and secondary-carephysicians, then with international experts. All corrections and suggestions by each member were shared with the rest of the task force and included to reach final consensus. The final version was validated by the steering committee. Results: The definition and indication of several types of knee orthoses (sleeve, patello-femoral, hinged or unicompartmental offloading braces) were detailed. Orthoses may be proposed in addition to first-line non-pharmacological treatment if patient acceptance is considered good. At every step, a specific clinical assessment is needed. Discussion/conclusion: Based on the latest high-level evidence, practice guidelines, and an expert panel, a DMT to facilitate daily practice prescription of knee orthoses for OA patients was designed. An evaluation of DMT implementation in a wide range of health professionals is still needed. (C) 2018 The Author(s). Published by Elsevier Masson SAS