The impact of a high versus a low glycaemic index breakfast cereal meal on verbal episodic memory in healthy adolescents

In this study, healthy adolescents consumed a) a low glycaemic index (G.I.) breakfast cereal meal, or b) a high G.I. breakfast cereal meal, before completing a test of verbal episodic memory in which the memory materials were encoded under conditions of divided attention. Analysis of remembering/forgetting indices revealed that the High G.I. breakfast group remembered significantly more items relative to the Low G.I. breakfast group after a long delay. The superior performance observed in the High G.I. group, relative to the Low G.I. group, may be due to the additional glucose availability provided by the high G.I. meal at the time of memory encoding. This increased glucose availability may be necessary for effective encoding under dual task conditions

A Versatile Pseudo-Random Noise Generator

A detailed design is presented for a digital pseudo-random noise generator. The instrument is built with standard integrated circuits. It produces both binary noise (pseudo-random binary sequences) and white Gaussian noise of variable bandwidth. By setting front panel switches to match tabulated octal codes, one may select a vast number of independent noise programs

Oligonucleotide sequences forming short self-complimentary hairpins can expedite the down-regulation of Coprinopsis cinerea genes

Gene silencing in fungi is often induced by dsRNA hairpin forming constructs the preparation of which can require multiple cloning steps. To simplify gene silencing in the filamentous fungi we have evaluated a high throughput cloning method for target sequences using the homobasidiomycete Coprinopsis cinerea, the GFP reporter and a commercially available vector system. The pSUPER RNAi System™, which was developed for mammalian experiments, exploits the human H1 Polymerase III (Pol III) RNA gene promoter and expedites cloning/expression of specific user-defined oligonucleotide sequences to form short self-complimentary hairpins. Transformation of C. cinerea with pSUPER constructs harboring specific oligonucleotides (19 nt stem length) enabled recovery of transformants with reduced transcripts of the GFP transgene, that were less fluorescent in protein assays and microscopic phenotypes. This technological advance should expedite functional genomic studies in C. cinerea and has wider potential for utility in other homobasidiomycete and filamentous fungi

The Yuma Territorial Prison Cemetery: Cold Cases of Grave Importance

Cemeteries, via grave markers and burial records, usually offer sufficiently scant data to enable a reconstruction of the communities they represent, but cemeteries of total institutions, here, the Yuma Territorial Prison, often yield even less data. With only the variables of ethnicity, sex, prisoner number, date of death, and cause of death, prison conditions were reconstructed for the 111 who died during the prison’s operation (1876-1909), and likely for the other 2,958 who were incarcerated there. First, prisoner number had a high, positive correlation with year of death, indicating that those who died in prison did not live long after incarceration. Further investigation found statistical dependence between the ethnicity of the prisoner and cause (and thus, manner) of death, with tuberculosis disproportionately effecting Hispanics and Native Americans, perhaps suggesting segregation by ethnicity. Hispanics were the only ones shot and killed attempting escape, though numerous escapes were attempted. Also statistically dependent was the decade of death by ethnicity, perhaps reflecting some ethnic sequence of incarceration. Finally, cause and manner of death, over time, were also dependent, likely reflecting deteriorating prison conditions. That so few variables can reveal so much refutes the adage that dead men tell no tales

Real-time observation of epitaxial graphene domain reorientation.

Graphene films grown by vapour deposition tend to be polycrystalline due to the nucleation and growth of islands with different in-plane orientations. Here, using low-energy electron microscopy, we find that micron-sized graphene islands on Ir(111) rotate to a preferred orientation during thermal annealing. We observe three alignment mechanisms: the simultaneous growth of aligned domains and dissolution of rotated domains, that is, 'ripening'; domain boundary motion within islands; and continuous lattice rotation of entire domains. By measuring the relative growth velocity of domains during ripening, we estimate that the driving force for alignment is on the order of 0.1 meV per C atom and increases with rotation angle. A simple model of the orientation-dependent energy associated with the moiré corrugation of the graphene sheet due to local variations in the graphene-substrate interaction reproduces the results. This work suggests new strategies for improving the van der Waals epitaxy of 2D materials

Evolution of diurnal surface winds and surface currents for Monterey Bay

The diurnal-period fluctuations of winds and surface currents are analyzed for September 1992 in and around Monterey Bay. Wind records are compared for three coastal stations and two mooring sites. Remotely-sensed surface current observations from two CODAR (HF radar) sites are used to explore the ocean's response to diurnal-period forcing. An average diurnal cycle is formed at each wind station and at all CODAR bins. The earliest sea breeze response is seen at the coastal wind stations where morning winds accelerate toward the coastal mountain ranges. A few hours later, the coastal winds accelerate to the southeast down the Salinas Valley. Offshore afternoon winds rotate from their normal alongshore orientation to also become aligned with the valley. The CODAR-derived surface currents respond in less than the two-hour sampling rate to the onset of the diurnal onshore winds. Currents accelerate in the direction of the Salinas Valley. As the day progresses, the more offshore currents rotate clockwise out from under the winds in a possible Ekman or inertial adjustment that continues throughout the night and spreads onshore. In the afternoon, a complicated eddy pattern develops near shore in a possible response to the coastal boundary.http://archive.org/details/evolutionofdiurn1094539684Lieutenant Commander, United States NavyApproved for public release; distribution is unlimited

Adapted Tricycle

This Final Design Review document describes the senior design project carried out by a team of four mechanical engineering students from California Polytechnic State University, San Luis Obispo in conjunction with California Children’s Services for Savannah, a student at San Luis Obispo High School. The purpose of the project is to design an adaptive vehicle for Savannah that serves as a form of exercise and can be easily operated by her with little to no outside assistance. Background into Savannah’s condition is provided as well as previous designs of similar adaptive tricycles, document standards and specifications which constrain design solutions, outline the scope of the project as well as the needs and wants of the end user as understood by the team, and develop a path towards the final design through description of the design process. The final design described in this document is centered around the user’s strongest muscle group (her abdomen and back muscles) to provide all necessary tricycle functions. These functions include steering, powering and braking. In general, the steering mechanism will utilize bevel gears to actuate the front wheel of the tricycle, the powering system will be a ratcheting push bar that is harnessed to the user’s torso, and the braking system will be a brake pad on the front wheel that is engaged by leaning back in the seat. This document contains our team’s process for developing our final design, solid model of our final design, justification calculations, manufacturing plans and engineering drawings, and our schedule for completion of the final product. In addition, a summary of the effects of the COVID-19 pandemic on project completion is provided, including an outline of future documentation which will aid an outside party in development and completion of our intended design, as well as the team’s revised project direction and scope

Characterization of serine proteinase expression in agaricus bisporus and coprinopsis cinerea by using green fluorescent protein and the A. bisporus SPR1 Promoter

The Agaricus bisporus serine proteinase 1 (SPR1) appears to be significant in both mycelial nutrition and senescence of the fruiting body. We report on the construction of an SPR promoter::green fluorescent protein (GFP) fusion cassette, pGreen_hph1_SPR_GFP, for the investigation of temporal and developmental expression of SPR1 in homobasidiomycetes and to determine how expression is linked to physiological and environmental stimuli. Monitoring of A. bisporus pGreen_hph1_SPR_GFP transformants on media rich in ammonia or containing different nitrogen sources demonstrated that SPR1 is produced in response to available nitrogen. In A. bisporus fruiting bodies, GFP activity was localized to the stipe of postharvest senescing sporophores. pGreen_hph1_SPR_GFP was also transformed into the model basidiomycete Coprinopsis cinerea. Endogenous C. cinerea proteinase activity was profiled during liquid culture and fruiting body development. Maximum activity was observed in the mature cap, while activity dropped during autolysis. Analysis of the C. cinerea genome revealed seven genes showing significant homology to the A. bisporus SPR1 and SPR2 genes. These genes contain the aspartic acid, histidine, and serine residues common to serine proteinases. Analysis of the promoter regions revealed at least one CreA and several AreA regulatory motifs in all sequences. Fruiting was induced in C. cinerea dikaryons, and fluorescence was determined in different developmental stages. GFP expression was observed throughout the life cycle, demonstrating that serine proteinase can be active in all stages of C. cinerea fruiting body development. Serine proteinase expression (GFP fluorescence) was most concentrated during development of young tissue, which may be indicative of high protein turnover during cell differentiatio

Cross-Resistance and Resistance Longevity as Induced by Bean Leaf Beetle, Cerotoma trifurcata and Soybean Looper, Pseudoplusia includens herbivory on Soybean

Cross-resistance, and longevity of resistance, induced by the bean leaf beetle, Cerotoma trifurcata, was studied IN the soybean PI 227687 that exhibited induced response in earlier studies. Bean leaf beetle adults and soybean looper, Pseudoplusia includens, larvae were used to induce resistance and to determine beetle feeding preference. Beetles were collected from soybean fields 2 to 5 days prior to the feeding preference test. The level of cross-resistance induced by soybean looper herbivory to subsequent bean leaf beetle feeding was higher when compared to cross-resistance induced by bean leaf beetle herbivory against subsequent feeding by soybean looper. Further, herbivory by the bean leaf beetle also induced resistance against soybean looper feeding. In the longevity study, leaflets from treated plants were collected 5, 10, 12, 14, 16, 20 and 25 days after initiation of feeding. Pairwise comparisons of leaflets from plants treated by bean leaf beetle herbivory with untreated plants revealed that induced responses were highest 14 and lowest 25 days after initiation of feeding. On other sampling days, levels of induced response varied with the sampling day

Locating air leaks in manned spacecraft using structure-borne noise

All manned spacecraft are vulnerable to leaks generated by micrometeorite or debris impacts. Methods for locating such leaks using leak-generated, structure-borne ultrasonic noise are discussed and demonstrated. Cross-correlations of ultrasonic noise waveforms from a leak into vacuum are used to find the location of the leak. Four methods for sensing and processing leak noise have been developed and tested and each of these can be used to reveal the leak location. The methods, based on phased-array, distributed sensor, and dual sensor approaches, utilize the propagation patterns of guided ultrasonic Lamb waves in the spacecraft skin structure to find the source or direction of the leak noise. It is shown that each method can be used to successfully locate the leak to within a few millimeters on a 0.6-m2 aluminum plate. The relative merits of the four methods are discussed