Cornea organoids from human induced pluripotent stem cells.

The cornea is the transparent outermost surface of the eye, consisting of a stratified epithelium, a collagenous stroma and an innermost single-cell layered endothelium and providing 2/3 of the refractive power of the eye. Multiple diseases of the cornea arise from genetic defects where the ultimate phenotype can be influenced by cross talk between the cell types and the extracellular matrix. Cell culture modeling of diseases can benefit from cornea organoids that include multiple corneal cell types and extracellular matrices. Here we present human iPS cell-derived organoids through sequential rounds of differentiation programs. These organoids share features of the developing cornea, harboring three distinct cell types with expression of key epithelial, stromal and endothelial cell markers. Cornea organoid cultures provide a powerful 3D model system for investigating corneal developmental processes and their disruptions in diseased conditions

Leucocyte numbers in normal and dwarf beef cattle before and after insulin injection

This bulletin reports on Missouri Agricultural Experiment Station research project 198, Cattle Improvement--P. [2].Includes bibliographical references (page 31)

Blood sugar level in normal and dwarf beef cattle before and after insulin injections

Digitized 2007 AES.Includes bibliographical references (pages 30-31)

Estate planning: important changes in tax law

https://egrove.olemiss.edu/dl_dhs/1052/thumbnail.jp

Survival of pines on droughty soils: two-year results

Three species of pines (loblolly, slash and longleaf) were planted with four treatments (loblolly and slash = bareroot, clay dip slurry and TerrasorH ; longleaf = containerized) to test survival on droughty, typic quartzipsamments soils. At the end of the first season, survival~s significantly better for the containerized longleaf and Terrasoro treated loblolly pine (81 and 85%) followed by untreaRed loblolly pine (51%), clay-treated loblolly (50%), then Terrasorb treated slash (48%), untreated slash (41%) and clay-treated slash (36%). At the e~of the second growing season, longleaf had 56% survival, Terrasor~ treated loblolly (51%), clay-dip slurry loblolly (31.9%), Terrasoro treated slash (21%), untreated loblolly pine (20%) and clay-treated slash and untreated slash (17%). Pest management recommendations and management considerations are presented

Timing of extension in the Pioneer metamorphic core complex with implications for the spatial-temporal pattern of Cenozoic extension and exhumation in the northern U.S. Cordillera

The Pioneer core complex (PCC) in central Idaho lies along a transition between Early Eocene and ca. 40 Ma core complexes to the north and south, respectively. Thus, the age of extensional development of the PCC is important in understanding the spatial-temporal patterns of core-complex development in the North American Cordillera. New results, including structural observations and U-Pb zircon (SHRIMP and ICPMS) geochronology, constrain the early extensional history of the footwall for the first time. High-temperature strain with a top-WNW shear-sense is pervasive throughout metamorphic rocks of the northwestern footwall. An isoclinally folded dike yields a crystallization age of ∼48-47 Ma, whereas a crosscutting dike yielded an age of 46 Ma. Metamorphic rocks are also intruded by the ∼50-48 Ma Pioneer intrusive suite (PIS), a W-dipping granodiorite sheet displaying a magmatic fabric. Northwest-trending lineations are locally visible and also defined by anisotropy of magnetic susceptibility, indicating that during emplacement, the PIS was undergoing similarly oriented extensional strain as the enclosing metamorphic rocks. Therefore, WNW-directed extension spanning this structural section occurred between ∼50 and 46 Ma. Following emplacement of crosscutting 46 Ma dikes, deformation was partitioned into the WNW-directed Wildhorse detachment. Motion on the detachment occurred between ∼38 and 33 Ma, as documented by previous 40Ar/ 39Ar thermochronology. It is not clear, however, whether extension was continuous through the interval between these two time periods. Although Early Eocene extension in the PCC was synchronous with extension in core complexes to the north, rates of footwall exhumation in central Idaho were much lower. This southward slowing is compatible with N-S differences in inferred subduction zone geometry/kinematics and in the internal character of the orogenic wedge

Identification of Brush Species and Herbicide Effect Assessment in Southern Texas Using an Unoccupied Aerial System (UAS)

Cultivation and grazing since the mid-nineteenth century in Texas has caused dramatic changes in grassland vegetation. Among these changes is the encroachment of native and introduced brush species. The distribution and quantity of brush can affect livestock production and water holding capacity of soil. Still, at the same time, brush can improve carbon sequestration and enhance agritourism and real estate value. The accurate identification of brush species and their distribution over large land tracts are important in developing brush management plans which may include herbicide application decisions. Near-real-time imaging and analyses of brush using an Unoccupied Aerial System (UAS) is a powerful tool to achieve such tasks. The use of multispectral imagery collected by a UAS to estimate the efficacy of herbicide treatment on noxious brush has not been evaluated previously. There has been no previous comparison of band combinations and pixel- and object-based methods to determine the best methodology for discrimination and classification of noxious brush species with Random Forest (RF) classification. In this study, two rangelands in southern Texas with encroachment of huisache (Vachellia farnesianna [L.] Wight & Arn.) and honey mesquite (Prosopis glandulosa Torr. var. glandulosa) were studied. Two study sites were flown with an eBee X fixed-wing to collect UAS images with four bands (Green, Red, Red-Edge, and Near-infrared) and ground truth data points pre- and post-herbicide application to study the herbicide effect on brush. Post-herbicide data were collected one year after herbicide application. Pixel-based and object-based RF classifications were used to identify brush in orthomosaic images generated from UAS images. The classification had an overall accuracy in the range 83–96%, and object-based classification had better results than pixel-based classification since object-based classification had the highest overall accuracy in both sites at 96%. The UAS image was useful for assessing herbicide efficacy by calculating canopy change after herbicide treatment. Different effects of herbicides and application rates on brush defoliation were measured by comparing canopy change in herbicide treatment zones. UAS-derived multispectral imagery can be used to identify brush species in rangelands and aid in objectively assessing the herbicide effect on brush encroachment

Decomposition Analyses Applied to a Complex Ultradian Biorhythm: The Oscillating NADH Oxidase Activity of Plasma Membranes Having a Potential Time-Keeping (Clock) Function

Seasonal decomposition analyses were applied to the statistical evaluation of an oscillating activity for a plasma membrane NADH oxidase activity with a temperature compensated period of 24 min. The decomposition fits were used to validate the cyclic oscillatory pattern. Three measured values, average percentage error (MAPE), a measure of the periodic oscillation, mean average deviation (MAD), a measure of the absolute average deviations from the fitted values, and mean standard deviation (MSD), the measure of standard deviation from the fitted values plus R-squared and the Henriksson-Merton p value were used to evaluate accuracy. Decomposition was carried out by fitting a trend line to the data, then detrending the data if necessary, by subtracting the trend component. The data, with or without detrending, were then smoothed by subtracting a centered moving average of length equal to the period length determined by Fourier analysis. Finally, the time series were decomposed into cyclic and error components. The findings not only validate the periodic nature of the major oscillations but suggest, as well, that the minor intervening fluctuations also recur within each period with a reproducible pattern of recurrence. biological clockdecomposition analysesultradian rhythmtemperature compensationcircadian rhythmcultured cells