Variabilidade genética de duas populações de Astyanax altiparanae da bacia do alto rio Paraná.

O presente trabalho tem como objetivo caracterizar a estrutura genética de duas populações de A. altiparanae da bacia do alto rio Paraná (Paranapanema e Tietê) através da análise de 11 marcadores moleculares do tipo microssatélite.Organizado por: Sílvio Ricardo Maurano; AQUACIÊNCIA 2012

Study of the urban evolution of Brasilia with the use of LANDSAT data

The urban growth of Brasilia within the last ten years is analyzed with special emphasis on the utilization of remote sensing orbital data and automatic image processing. The urban spatial structure and the monitoring of its temporal changes were focused in a whole and dynamic way by the utilization of MSS-LANDSAT images for June 1973, 1978 and 1983. In order to aid data interpretation, a registration algorithm implemented at the Interactive Multispectral Image Analysis System (IMAGE-100) was utilized aiming at the overlap of multitemporal images. The utilization of suitable digital filters, combined with the images overlap, allowed a rapid identification of areas of possible urban growth and oriented the field work. The results obtained permitted an evaluation of the urban growth of Brasilia, taking as reference the proposed stated for the construction of the city

A new emerging oral infection: Raoultella planticola in a boy with haematological malignancy

BACKGROUND: Oral mucositis is a common complication in pediatric cancer patients, affecting up to 80% of children. Due to neutropenia and disruption of the mucosal barrier, chemotherapy-induced oral mucositis is often complicated by super-infections. CASE REPORT: A 16-years old male with stage 3 Burkitt's lymphoma developed chemotherapy induced oral mucositis grade 3 (according to WHO scale). Ulcers were quickly growing (reaching a maximum diameter of 3 cm) and became greyish in colour, resulting in dysphagia and pain. A swab of the lesions was taken and microbiological tests were performed. The sample grew for Raoultella planticola, an encapsulated Gram-negative bacterium whose full pathogenic potential still needs to be defined. TREATMENT: The patient received antibiotic combination therapy with Amikacin and Ceftazidime for 8 days. Complete healing of the lesions and resolution of the symptoms were reached and he completed his antineoplastic therapy without further complications. FOLLOW-UP: Twelve months after the infection, he is alive and well, with no oral complaints. CONCLUSION: This is the first report of a Raoultella planticola infection in a patient with chemotherapy induced oral mucositis. This type of infection must be added to the list of organisms to be considered when caring for these patients

Reprodução induzida e criação de larvas de híbridos interespecíficos e intragenérico da ordem siluriforme (Pisces).

O presente trabalho tem como objetivo analisar a resposta à indução artificial da reprodução em exemplares de cachapinta, híbrido resultante do cruzamento de Pseudoplatystoma corruscans X Pseudoplatystoma reticulatum no cruzamento intergenérico entre fêmea deste produto híbrido e macho de jundiá (Leiarius marmoratus), para verificar a produção de gametas viáveis, quantidade de larvas eclodidas, percentual de sobrevivência e crescimento das larvas e consequentemente a produção de exemplares para estudos genéticos.Organizado por: Sílvio Ricardo Maurano; AQUACIÊNCIA 2012

Determinação de polifenóis como subsídio para seleção de variedades do Banco Ativo de Germoplasma de Uva.

A proposta do presente trabalho é analisar os teores de polifenóis bioativos em variedades do Banco Ativo de Germoplasma (acessos) e/ou novas variedades em fase de criação (seleções), através da técnica de Cromatografia Líquida de Alta Eficiência (HPLC)

Verification of the selectivity of a liquid chromatography method for determination of stilbenes and flavonols in red wines by mass spectrometry.

Quantification of bioactive phenols, like stilbenes and flavonols (SaF), has been conducted to evaluate the nutraceutical potential of red wines. However, there is still a lack of full validated, fast and accessible liquid chromatography methods offering high selectivity and a simple procedure. We present here the use of a high-resolution mass spectrometer to evaluate the selectivity of a feasible and traditional liquid chromatography technique (HPLC? DAD) to analyze markers of aglycone SaF in red wines. The SaF compounds were tested: trans-resveratrol, trans-eviniferin, quercetin, myricetin, and kaempferol, as well as trans-cinnamic acid, one of their precursors. System suitability and validation tests were employed for the selected conditions (octylsilane column, methanol mobile phase, and gradient elution). The validation process ensured the HPLC?DAD method was selective, linear, sensitive, precise, accurate and robust. The method was then applied to red wine samples from the Campanha Gau´cha region, Southern Brazil. The real samples contained different SaF levels, showing that the method is applicable to routine use. Furthermore, this was the first SaF characterization of red wines from the Campanha Gau´cha, contributing to regional and product development. Keywords Bioactive phenols Red wine Liquid chromatography Mass spectrometry Validatio

Atropisomerism in Hindered Naphthyl Sulfoxides: Structure, Stereodynamics, and Chiral Resolution.

Barriers for the EZ interconversion of atropisomers of 1-naphthyl sulfoxides (ArSOR) having a methyl group at position 2 of the naphthalene moiety were measured by variable-temperature NMR. Their values were found to cover the range 10.6-18.4 kcal mol-1, the extreme values corresponding to derivatives 1 (R = Me) and 4 (R = Bu(t)), respectively. NOE and LIS measurements indicated that the Z atropisomer is more stable than the E but that the absence of the methyl group at position 2 of the naphthalene moiety reverses this trend, rendering E more stable than Z. Solid-state NMR and X-ray diffraction of 4 established that only the more stable atropisomer (Z) is present in the crystalline state. Molecular mechanics calculations suggest that the Z,E interconversion process might occur by a rotation pathway having an opposite direction in the case of the more hindered derivatives 3 and 4 (R = Pr(i) and Bu(t), respectively) with respect to the less hindered 1 and 2 (R = Me and Et, respectively). The enantiomers, which are due to the presence of the asymmetric sulfur atom, were resolved on a chiral stationary phase (DACH-DNB) having an SS configuration. Asymmetric oxidation reactions were employed to assign the absolute R configuration to the more retained enantiomers of alkyl aryl sulfoxides. The opposite trend (S being retained longer) was observed for diaryl sulfoxides such as 5 (R = Ph). In the case of the derivative with the largest interconversion barrier, sulfoxide 4, it was also possible to resolve (at -35-degrees-C) the two enantiomeric forms and their associated atropisomers. The use of on-line CD detection and the knowledge of the NMR assignments allowed us to unambiguously assign the elution order of the four species as ES, ER, ZS, ZR

A RAPD-PCR-based genetic diversity analysis of Helicoverpa armigera and H. zea populations in Brazil.

Therefore, the aim of this study was to determine the genetic diversity of H. armigera and H. zea populations by RAPD-PCR analysis. The most important result was the clustering of one H. armigera population in a group predominantly formed by H. zea. It could indicate a possible occurrence of an interspecific cross between these species. This is a concern to Brazilian agriculture due to the possibility of selection of hybrids well adapted to the American environment, which would be inherited from H. zea