Determinacions del perfil genètic de tumors sòlids de l’adult

Perfil genètic; Tumors sòlids; Adults; PrecisióPerfil genético; Tumores sólidos; Adultos; PrecisiónGenetic profile; Solid tumors; Adults; AccuracyEn aquest estudi s’ha definit la llista de gens per a cada patologia i tots ells han estat seleccionats atenent a; la seva utilitat diagnòstica per definir els subtipus tumorals en localitzacions tumorals molt concretes; la seva utilitat pronòstica i predictiva, sempre que això comporti un canvi d’actitud terapèutica; la seva utilitat terapèutica per a la indicació de l’ús de fàrmacs diana

A view of the Brazil-Malvinas confluence, March 2015

The encountering of the subtropical Brazil Current (BC) and the subantarctic Malvinas Current (MC) along the western margin of the Argentine Basin forms the Brazil-Malvinas Confluence (BMC), one of the most intense open-ocean fronts in the world ocean and a site for the formation of intermediate water masses. Here, we provide a comprehensive description of the BMC based on physical and biogeochemical data – hydrographic stations, profiling floats and subsurface drifters – gathered in March 2015. We use these data in order to characterize the impinging and outflowing currents and to describe the cross- and along-frontal thermohaline structure. In addition, we compare the in-situ measurements with both climatological data and the Mercator Ocean eddy-resolving reanalysis. The hydrographic sections illustrate the contrasting properties between the two western boundary currents: warm, salty, nutrient- and oxygen-poor oligotrophic subtropical waters carried southward by the BC and the cold, fresh, oxygen- and nutrient-rich subantarctic waters carried northward by the MC. The frontal system is also characterized by the presence of thermohaline intrusions, with the cross-frontal gradients and along-front velocities sharpening as the colliding currents shape the frontal system. We also observe brackish waters spreading on top of the frontal jet as a result of both the confluence dynamics and off-shelf advection favored by north-easterly winds. These low-salinity waters are positively correlated with surface ageostrophic speeds over the frontal jet. The cruise data illustrates the high regional and mesoscale variability as compared with climatological conditions, and further document the submesoscale subsurface complexity, which is not properly captured by available operational models.Fil: Orúe Echevarría, Dorleta. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Pelegrí, Josep L.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Alonso González, Iván J.. Oceomic, Marine Bio And Technology S.L; EspañaFil: Benítez Barrios, Verónica M.. Oceomic, Marine Bio And Technology S.L; EspañaFil: Emelianov, Mikhail. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: García Olivares, Antonio. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Gasser i Rubinat, Marc. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: De La Fuente, Patricia. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Herrero, Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Isern Fontanet, Jordi. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Masdeu Navarro, Marta. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Peña Izquierdo, Jesús. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Piola, Alberto Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Ramírez Garrido, Sergio. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Rosell Fieschi, Miquel. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Salvador, Joaquín. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Saraceno, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; Argentina. Universidad de Barcelona; EspañaFil: Valla, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias de la Atmósfera y los Océanos; ArgentinaFil: Vallès Casanova, Ignasi. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Vidal, Montserrat. Universidad de Barcelona; Españ

Determinacions del perfil genètic de tumors sòlids de l’adult

Perfil genètic; Tumors sòlids; Adults; PrecisióPerfil genético; Tumores sólidos; Adultos; PrecisiónGenetic profile; Solid tumors; Adults; AccuracyEn aquest estudi s’ha definit la llista de gens per a cada patologia i tots ells han estat seleccionats atenent a; la seva utilitat diagnòstica per definir els subtipus tumorals en localitzacions tumorals molt concretes; la seva utilitat pronòstica i predictiva, sempre que això comporti un canvi d’actitud terapèutica; la seva utilitat terapèutica per a la indicació de l’ús de fàrmacs diana

Ambientalitació del camp de golf PGA Catalunya

Estudi del camp de golf PGA Catalunya de Caldes de Malavella, l’aigua de reg, ús de models nòrdics poc adequats al clima mediterrani, tipus de gespa i integració del camp al medi. Es proposen una sèrie de mesures per tal de millorar l’eficiència dels diferents processos i obtenir així un benefici ambiental i econòmi

Seasonality of biogeochemically relevant genes in the NW Mediterranean coastal microbiome

16 Symposium of Aquatic Microbial Ecology (SAME16), “From Boat to Bench”- Integrating field observation with lab experiments, 1-6 September 2019, Potsdam, Germany.-- 1 pageMarine biogeochemical processes are mediated by microorganisms through protein encoding genes, some being solely present in prokaryotes. During the last decade, genomic site-specific and large-scale expeditions have discovered millions of genes, unveiling new putative functions and large gene heterogeneity. Nonetheless, little still is known about the genomic basis of key biogeochemical processes, the taxonomic groups mediating them and whether seasonal patterns occur across taxonomic levels. For these reasons, we conducted temporal analyses of the functional diversity for 18 key functional genes in a model coastal marine microbiome. We analyzed a 7- year metagenomic time-series from the Blanes Bay Microbial Observatory (NW Mediterranean Sea) through state-of-the-art omics’ tools and time series statistics. This dataset allowed us to explore the seasonal trends and gene heterogeneity in various key genes involved in the four major marine biogeochemical cycles (carbon, phosphorus, sulfur and nitrogen). Preliminary results show some key functions as seasonal, although with heterogeneity across the different taxonomical ranks. Our results define the seasonality of gene presence in that coastal environment and are the basis to discuss the implications of the seasonality of such genes for ecosystem functioningPeer Reviewe

Seasonality of biogeochemically relevant microbial genes in a coastal ocean microbiome

19 pages, 6 figures, 1 table, supporting information https://doi.org/10.1111/1462-2920.16367.-- Data Availability Statement: All the code used for the analyses can be found in the following repository: https://github.com/adriaaulaICM/key_biogem_genes. The data to reproduce the analyses can be found in the same repository. The reader can find in the data folder a FASTA file for all the gene variants, the abundance count table, the taxonomic assignation (both with GTDB and Uniref databases), the functional annotation for each variant, and the seasonality testMicrobes drive the biogeochemical cycles of marine ecosystems through their vast metabolic diversity. While we have a fairly good understanding of the spatial distribution of these metabolic processes in various ecosystems, less is known about their seasonal dynamics. We investigated the annual patterns of 21 biogeochemical relevant functions in an oligotrophic coastal ocean site by analysing the presence of key genes, analysing high-rank gene taxonomy and the dynamics of nucleotide variants. Most genes presented seasonality: photoheterotrophic processes were enriched during spring, phosphorous-related genes were dominant during summer, coinciding with potential phosphate limitation, and assimilatory nitrate reductases appeared mostly during summer and autumn, correlating negatively with nitrate availability. Additionally, we identified the main taxa driving each function at each season and described the role of underrecognized taxa such as Litoricolaceae in carbon fixation (rbcL), urea degradation (ureC), and CO oxidation (coxL). Finally, the seasonality of single variants of some families presented a decoupling between the taxonomic abundance patterns and the functional gene patterns, implying functional specialization of the different genera. Our study unveils the seasonality of key biogeochemical functions and the main taxonomic groups that harbour these relevant functions in a coastal ocean ecosystemThis research was funded by grants REMEI (CTM2015-70340-R), MIAU (RTI2018-101025-B-I00), and ECLIPSE (PID2019-110128RB-100) from the Spanish Ministry of Science and Innovation, and received support from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000924-S). Adrià Auladell was supported by a Spanish FPI grant from the Ministry of Science and Innovation. Isabel Ferrera had the support of a ‘2019 Leonardo Grant for Researchers and Cultural Creators’ from the BBVA FoundationPeer reviewe

Long-Term Synchrony, Rhythmicity, and Functional Redundancy in Marine Microbiomes

ASLO Aquatic Sciences Meeting, Resilience and Recovery in Aquatic Systems, 4-9 June 2023, Palma de Mallorca, SpainThe amount of functional redundancy in microbiomes is a matter of debate. Investigating the synchrony and the rhythmicity in the temporal dynamics of microbes populating environmentally similar locations can provide new insights into functional redundancy. When comparing the dynamics of neighbouring microbiomes, high functional redundancy could be depicted by cases where metabolic functions are synchronous over time while the genes or taxa contributing to those functions are not. In turn, low functional redundancy would be exemplified by cases where both functions and genes or taxa are synchronous over time. The same behaviour is predicted for the rhythmicity in each location. Here we investigate the amount of synchrony and rhythmicity at the functional, gene, and taxon levels over 7 years in monthly metagenomes from two marine-coastal microbiomes in the Mediterranean Sea separated by ~150 km and connected by a dominant south-west marine current. We found functions, genes, and taxa showing high, low, or anti-synchrony, as well as rhythmic and arhythmic patterns. The distribution of functions, genes, and taxa displayed an average rhythmic pattern (PnMax > 8). Yet, altogether, they showed low average synchrony (Sy~0.25). The analysis of 45 key biogeochemical functions indicated high synchrony and rhythmicity in some functions even when the most abundant corresponding genes had low synchrony and rhythmicity, pointing to functional redundancy in crucial metabolismsPeer reviewe

Global metagenomics reveals a widespread fungal component of the deep-ocean microbiome

Association for the Sciences of Limnology and Oceanography (ASLO) Aquatic Sciences Meeting, Mountains of the Sea, 26 February - 3 March 2017, Honolulu, Hawai`iThe deep ocean is the largest habitat on Earth but our knowledge about its microbiome, microeukaryotes in particular, is limited. Here we investigate a deep-sea fungal Metagenome-Assembled Genome (MAG) with a widespread distribution and a relatively high abundance in specific locations. A total of 83 globally distributed metagenomes recovered from 1,500m to 4,000m depth, which were part of the Malaspina 2010 expedition, were co-assembled and binned using different algorithms. We retrieved 15.61Mb of a putative yeast, phylogenetically related to Tilletiopsis spp., featuring ca. 80% of genome recovery. Gene prediction based on conserved orthologs indicated 4,370 genes, which were annotated using a number of databases. In particular, a total of 2,846 genes were annotated using KEGG, with most matching orthologs originating from other fungi (Pseudozyma flocculosa [47.4%], Ustilago maydis [37.5%] and Malassezia globosa [3%]). Functions related to membrane transporters, endocytosis and potential pathogenicity as well as lysosome-like proteins are currently being analysed to understand the ecology of this uncharacterized fungi. Analyses of 18S rDNA and rRNA amplicons from 13 globally-distributed vertical profiles pointed to geographically heterogeneous abundances that tended to increase with depth. In particular, MAG rRNA detection in the deep ocean points to an active metabolism. Analyses of SNPs and indels indicate fine¿grained genomic change that could reveal ecotypic variation. Overall, our results provide insight on the functional role of fungi in the deep oceanPeer Reviewe