Desempenho produtivo e parâmetros sanguíneos de vacas leiteiras em pastejo suplementadas com resíduos de feijão

The objective of this work was to evaluate the effect of dietary supplement bean residue on growth performance and blood parameters of dairy cows grazing. Eight crossbred cows weighing 500 kg were analyzed, distributed in 4×4 double Latin square, and fed with 134, 240, 348, and 449 g kg‑1 of a supplementary diet of bean waste in the concentrated feed. The animals received the supplementation after milking in the morning and in the afternoon. The inclusion of bean residues did not change average body weights and body scores; however, it reduced the milk production. Fat, protein, lactose, and total solid contents (g kg‑1) were not changed by bean waste inclusion. Blood glycose and cholesterol did not change before the offering of the concentrated feed diet, nor after four to six hours of feeding this diet. The inclusion of bean residues in the concentrated feed of dairy cows subjected to grazing, with a daily average production of 13 kg, reduces milk production. However, the use of these residues has little effect on milk composition and blood parameters.O objetivo deste trabalho foi avaliar o efeito da alimentação suplementar de resíduos de feijão no desempenho produtivo e nos parâmetros sanguíneos de vacas leiteiras em pastejo. Foram utilizadas oito vacas mestiças, com peso médio de 500 kg, distribuídas em duplo quadrado latino 4×4, alimentadas com alimentação suplementar de 134, 240, 348 e 449 g kg‑1 de resíduos de feijão na ração concentrada. Os animais receberam a suplementação após a ordenha no período da manhã e da tarde. A inclusão de resíduos de feijão não alterou os pesos corporais médios e os escores corporais; no entanto, reduziu a produção de leite. Os teores de gordura, proteína bruta, lactose e extrato seco total não foram alterados pela inclusão dos resíduos de feijão. A concentração sanguínea de glicose e colesterol não foi alterada pela adição do resíduo antes do fornecimento de ração concentrada e nem quatro e seis horas após essa alimentação. A inclusão dos resíduos de feijão na ração concentrada de vacas leiteiras em pastejo, com produção média diária de 13 kg, reduz a produção de leite. Todavia, a utilização desses resíduos pouco influencia a composição do leite e os parâmetros sanguíneos

Zika virus infection among symptomatic patients from two healthcare centers in Sao Paulo State, Brazil: prevalence, clinical characteristics, viral detection in body fluids and serodynamics.

Zika virus (ZIKV) clinical presentation and frequency/duration of shedding need further clarification. Symptomatic ZIKV-infected individuals identified in two hospitals in Sao Paulo State, Brazil, were investigated regarding clinical characteristics, shedding in body fluids, and serodynamics. Ninety-four of 235 symptomatic patients (Site A: 58%; Site B: 16%) had Real-Time PCR-confirmed ZIKV infection; fever, headache and gastrointestinal symptoms were less frequent, and rash was more frequent compared to ZIKV-negative patients. Real-Time PCR in serum had worse performance compared to plasma, while urine had the highest sensitivity. Shedding in genital fluids and saliva was rare. IgM positivity was the highest 28 days (24%); IgG positivity increased >14 days (96%) remaining positive in 94% of patients >28 days. ZIKV prevalence varied importantly in two neighboring cities during the same transmission season. Urine Real-Time PCR can improve diagnostic sensitivity; serum testing is less useful. Accurate serological tests are needed to improve diagnosis and surveillance

Zika virus infection among symptomatic patients from two healthcare centers in Sao Paulo State, Brazil: prevalence, clinical characteristics, viral detection in body fluids and serodynamics

Zika virus (ZIKV) clinical presentation and frequency/duration of shedding need further clarification. Symptomatic ZIKV-infected individuals identified in two hospitals in Sao Paulo State, Brazil, were investigated regarding clinical characteristics, shedding in body fluids, and serodynamics. Ninety-four of 235 symptomatic patients (Site A: 58%; Site B: 16%) had Real-Time PCR-confirmed ZIKV infection; fever, headache and gastrointestinal symptoms were less frequent, and rash was more frequent compared to ZIKV-negative patients. Real-Time PCR in serum had worse performance compared to plasma, while urine had the highest sensitivity. Shedding in genital fluids and saliva was rare. IgM positivity was the highest <14 days after the symptoms onset (86%), decreasing >28 days (24%); IgG positivity increased >14 days (96%) remaining positive in 94% of patients >28 days. ZIKV prevalence varied importantly in two neighboring cities during the same transmission season. Urine Real-Time PCR can improve diagnostic sensitivity; serum testing is less useful. Accurate serological tests are needed to improve diagnosis and surveillance

estudos artísticos

Crescer na intervenção e na comunicação é um dos objetivos da Revista Gama, estudos artísticos. Promove-se a comunicação, formal, dentro das regras da comunicação académica, através de textos cuja característica comum é serem escritos por artistas, sobre a obra de outros artistas. Este foi o critério base que inspirou o projeto das iniciativas associadas ao Congresso CSO (criadores sobre outras obras), que já completou seis anos de disseminação. A Revista Gama singularizou-se por convocar artistas e obras que de algum modo estariam esquecidos, desconhecidos, ou ainda pouco divulgados. Obras cuja execução tem raízes em passados mais ou menos recentes, mas que pelo excesso de discursos na contemporaneidade, não obtiveram a divulgação desejada. Este é um propósito de intervenção no conhecimento patrimonial: as obras existem, foram executadas, enriquecem o nosso património, mas há que as fazer funcionar, dar a conhecer, aos outros artistas, aos especialistas, ao grande público. Assim se reuniram neste número 6 da Revista Gama vinte e quatro artigos originais, procurando-se, na sua sequência e articulação, algumas relações de pertinência e afinidade. Olhares sobre arquivos, sobre acervos, sobre coleções, conjuntos muitas vezes fechados e em perigo de esquecimento, ou de incompreensão: uma entrada discreta que se abre para o interior de uma câmara escura, que é um espaço cheio de imagens por revelar.info:eu-repo/semantics/publishedVersio

O Serviço de documentação textual e iconografia do Museu Paulista

The essay compares the curatorship's works realized during the decade of 1990 by the actual Department of Textual and Iconographical Documentation of Museu Paulista, responsible for the MP Fund / Permanent File (Fundo MP/Arquivo Permanente), hundreds of collections and textual funds and 50.000 iconography pieces, great part of which are gathered in photographic collections. It shows how the documentation work extrapolates the limits of SVDHICO in order to integrate itself with the group activities of the museum and with other research groups. It also points towards new work methodologies which allow to perform the curatorship in an integrated way with the interdisciplinary research and the culture diffusion.O artigo faz um balanço dos trabalhos de curadoria realizados durante a década de 1990 pelo atual Serviço de Documentação Textual e Iconografia do Museu Paulista, responsável pelo Fundo MP/Arquivo Permanente, centenas de coleções e fundos textuais e 50.000 peças de iconografia, grande parte delas reunidas em coleções fotográficas. Mostra como o trabalho de documentação extrapola os limites do SVDHICO para integrar-se com as atividades de conjunto do Museu e com outros grupos de pesquisa. Aponta também para novas metodologias de trabalho com imagens que permitem realizar a curadoria de forma integrada à pesquisa interdisciplinar e à difusão cultural

Função lútea em adolescentes normais com ciclos menstruais regulares Luteal function in normal adolescents with regular menstrual cycles

OBJETIVO: examinar a função lútea em adolescentes com ciclos menstruais regulares. MÉTODOS: foram incluídas neste estudo, de coorte prospectivo, 55 adolescentes eumenorrêicas, com idade entre 14 e 19 anos e menarca aos 12,2 anos. Examinaram-se o dia da ocorrência de ovulação, o grau de vascularização do corpo lúteo e o índice de resistência dos vasos ovarianos, as concentrações de progesterona e a resposta do endométrio. A ovulação foi identificada por ultra-sonografia transvaginal a partir do 2º ou 5º dia do ciclo, repetindo-a a cada dois dias. A vascularização e o índice de resistência lútea foram aferidos por dopplervelocimetria no 10º dia pós-ovulação. Os níveis de progesterona sérica foram estimados por quimioluminescência nos dias 6, 9 e 12 da fase lútea. A histologia endometrial foi analisada após biópsia feita entre o 8º e o 10º dia pós-ovulação. Usou-se software SPSS para análise estatística, assumindo-se significância quando p<0,05. RESULTADOS: A ovulação foi observada em média no 17º dia. Os níveis séricos de progesterona foram de 11,4, 10,9 e 3,9 ng/mL respectivamente nos dias 6, 9 e 12 pós-ovulação; a média dos níveis de progesterona nos três dias foi de 10,3 ng/ml. A vascularização do corpo lúteo foi escassa em 34,6%, moderada em 23,6% e exuberante em 41,8%. O índice de resistência foi de 0,441 dez dias após a ovulação. O endométrio mostrou-se sincrônico em 85,5% das biópsias e fora de fase em 14,5%. As correlações entre o dia da ovulação e o tipo de endométrio (p=0,29), níveis de progesterona e o tipo endométrio (p=0,454), grau de vascularização (p=0,99) e índice de resistência do corpo lúteo (p=0,23) não foram estatisticamente significantes. A histologia endometrial e o grau de vascularização também não mostraram associação (p=0,61). CONCLUSÃO: a função lútea em adolescentes eumenorrêicas, avaliada pela datação do endométrio em ciclo único, mostrou-se anormal em 14,5%. As variáveis grau e intensidade da vascularização, concentração sérica de progesterona ou dia da ocorrência de ovulação não mostraram associação com a histologia endometrial.<br>PURPOSE: to evaluate the luteal function in adolescents with regular menstrual cycles. METHODS: this prospective cohort study included 55 adolescents, aged 14-19 years, with menarche at 12.2 years. Ovulation was identified by ultrasound, starting on the second or fifth day of the cycle. The corpus luteum vascularization and the resistence index of the ovarian vessels were measured by Doppler on the tenth postovulatory day. Progesterone was measured by chemoluminescence on days 6, 9 and 12 of the luteal phase. The endometrial biopsy was performed 8 to 10 days after ovulation. The results were analyzed using the SPSS software and were considered significant when p<0.05. RESULTS: on average ovulation was on day 17. Progesterone levels were 11.4, 10.9 and 3.9 ng/mL on days 6, 9, and 12 after ovulation, respectively; the progesterone mean during the whole luteal phase was 10.3 ng/ml. Luteal vascularization was scarce in 34.6%, mild in 23.6% and exuberant in 41.8%. The resistance index was 0.441. On the tenth day post-ovulation the endometrium was normal in 85.5% and out-of-phase in 14.5%. There was no correlation between the ovulation day and endometrial dating (p=0.294), levels of progesterone and endometrial dating (p=0.454), progesterone and corpus luteum vascularization (p=0.994), or resistance index (p=0.237). There also was no association between endometrium development and degree of vascularization (p=0.611). CONCLUSION: abnormal luteal function in adolescents with regular menstrual cycles was found in 14.5%. Degree of vascularization, resistance index, and serum progesterone were not related to endometrium development

Aspectos ultra-estruturais do citomegalovírus humano: em órgãos fetais - baço, pulmão e cérebro

Made available in DSpace on 2011-11-01T16:37:05Z (GMT). No. of bitstreams: 2 license.txt: 1648 bytes, checksum: e095249ac7cacefbfe39684dfe45e706 (MD5) 1984 - Aspectos ultra-estruturais do citomegalovírus humano.pdf: 1262808 bytes, checksum: 7a4e78ac8ee8b9476a1add5d2c240a7d (MD5) Previous issue date: 1984Universidade Federal do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Instituto de Matemática. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Conselho de Ensino de Pós-graduação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Conselho de Ensino de Pós-graduação. Rio de Janeiro, RJ, Brasil.Um estudo ultra-estrutural de casos de doenças congênitas foi realizado em três órgãos (baço, pulmão, cérebro). Partículas herpéticas foram observadas tanto no núcleo como no citoplasma dos órgãos estudados. os aspectos ultra-estruturais foram destacados por seu caráter de importância no diagnóstico por seu caráter de importância no diagnóstico da infecção viral

Comparison of two smoking cessation interventions for inpatients

ABSTRACT Objective: This study aimed to compare the effectiveness of two cognitive behavioral therapy-based smoking cessation interventions initiated during hospitalization and to evaluate the factors related to relapse after discharge. Methods: This was a prospective randomized study involving 90 smokers hospitalized in a university hospital. We collected data related to sociodemographic characteristics, reasons for admission, smoking-related diseases, smoking history, the degree of nicotine dependence (ND), and the level of craving. Patients were divided into two treatment groups: brief intervention (BrInter, n = 45); and intensive intervention with presentation of an educational video (InInterV, n=45). To assess relapse, all patients were assessed by telephone interview in the first, third, and sixth months after discharge. Abstinence was confirmed by measurement of exhaled carbon monoxide (eCO). Results: Of the 90 patients evaluated, 55 (61.1%) were male. The mean age was 51.1 ± 12.2 years. The degree of ND was elevated in 39 (43.4%), and withdrawal symptoms were present in 53 (58.9%). The mean eCO at baseline was 4.8 ± 4.5 ppm. The eCO correlated positively with the degree of ND (r = 0.244; p = 0.02) and negatively with the number of smoke-free days (r = −0.284; p = 0.006). There were no differences between the groups in terms of the variables related to socioeconomic status, smoking history, or hospitalization. Of the 81 patients evaluated at 6 months, 33 (40.7%) remained abstinent (9 and 24 BrInter and InInterV group patients, respectively; p = 0.001), and 48 (59.3%) had relapsed (31 and 17 BrInter and InInterV group patients, respectively; p= 0.001). Moderate or intense craving was a significant independent risk factor for relapse, with a relative risk of 4.0 (95% CI: 1.5-10.7; p < 0.00001). Conclusions: The inclusion of an educational video proved effective in reducing relapse rates. Craving is a significant risk factor for relapse

Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection.

Vaccination is one the most important strategies for the prevention of visceral leishmaniasis (VL). In the current study, a new Leishmania hypothetical protein, LiHyP, which was previously showed as antigenic in an immunoproteomic search in canine VL, was evaluated regarding its immunogenicity and protective efficacy against Leishmania infantum infection. The effects of the immunization using LiHyP were evaluated when administered as a DNA plasmid (DNA LiHyP) or recombinant protein (rLiHyP) associated with saponin. The immunity elicited by both vaccination regimens reduced the parasitism in liver, spleen, bone marrow and draining lymph nodes, being associated with high levels of IFN-?, IL-12, GM-CSF, and specific IgG2a antibody, besides low production of IL-4, IL-10, and protein and parasite-specific IgG1 antibodies. CD4+ T cells contributed more significantly to IFN-? production in the rLiHyP/saponin group, while CD8+ T cells were more important in the production of this cytokine in the DNA LiHyP group. In addition, increased IFN-? secretion, along with low levels of IL-10, were found when PBMCs from treated VL subject and healthy individuals were stimulated with the recombinant protein. In conclusion, when administered either as a DNA plasmid or recombinant protein, LiHyP can direct the immune response towards a Th1 immune profile, protecting animals against L. infantum infection; therefore, it can be seen as a promising immunogen against human VL