Upper Respiratory Tract Microbiome and Otitis Media Intertalk : Lessons from the Literature

Otitis media (OM) is one of the most common diseases occurring during childhood. Microbiological investigations concerning this topic have been primarily focused on the four classical otopathogens (Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Streptococcus pyogenes) mainly because most of the studies have been conducted with culture-dependent methods. In recent years, the introduction of culture-independent techniques has allowed high-throughput investigation of entire bacterial communities, leading to a better comprehension of the role of resident flora in health and disease. The upper respiratory tract (URT) is a region of major interest in otitis media pathogenesis, as it could serve as a source of pathogens for the middle ear (ME). Studies conducted with culture-independent methods in the URT and ME have provided novel insights on the pathogenesis of middle ear diseases through the identification of both possible new causative agents and of potential protective bacteria, showing that imbalances in bacterial communities could influence the natural history of otitis media in children. The aim of this review is to examine available evidence in microbiome research and otitis media in the pediatric age, with a focus on its different phenotypes: acute otitis media, otitis media with effusion and chronic suppurative otitis media

O uso dos instrumentos da avaliação do risco da violência na Argentina e no Chile

Introducción: En las últimas décadas hubo importantes avances científicos en la sistematización de la evaluación del riesgo de violencia, pero esas contribuciones no se implementan de manera rápida ni uniforme en la práctica latinoamericana. Objetivos: Describir qué instrumentos de evaluación de riesgo de violencia se utilizan en Chile y Argentina, y qué características tiene esa práctica profesional. Material y método: Se realizó una encuesta a través de la web, a profesionales relacionados con la salud mental registrados en listas nacionales e internacionales de 17 países, entre septiembre y diciembre del 2012. En el estudio se informan resultados de 46 respondientes de Chile y Argentina. Resultados: El 78% de los psiquiatras, el 93% de los psicólogos y el 100% de los trabajadores sociales utilizaron instrumentos para ERV; la amplia mayoría los consideró relativamente útiles. Los instrumentos más utilizados fueron HCR-20, PCL-R y PCL-SV.Introduction: The last decades revealed important scientific advances in the systemization of violence risk assessment; nevertheless, the implementation in practice of these contributions have neither been fast nor uniform in Latin America. Objectives: The article is aimed at describing the instruments for violence risk assessment used in Chile and Argentina and the features of this professional practice. Material and method: A web survey was carried out among mental health-related professionals registered in the national and international lists of 17 countries between September and December 2012. The results obtained from 46 respondents from Chile and Argentina are reported in this study: 78% of the psychiatrists, 93% of the psychologists and 100% of the social workers involved have employed violence risk assessment (VRA) instruments deemed relatively useful by a wide majority, being HCR-20, PCL-R and PCL-SV the most commonly used.Introdução: Nas últimas décadas houve avanços científicos importantes na sistematização da avaliação do risco da violência, mas aquelas contribuições não são executadas rapidamente nem uniformemente na prática latinoamericana. Objetivos: Descrever que instrumentos da avaliação do risco da violência são usados no Chile e na Argentina, e que características tem essa prática profissional. Material e método: Uma pesquisa foi feita através da Web, aos profissionais relacionados à saúde mental registrados nas listas nacionais e internacionais de 17 países, entre setembro e dezembro do 2012. No estudo os resultados de 46 respondentes do Chile e da Argentina são informados. Resultados: 78% dos psiquiatra, 93% dos psicólogos e o 100% dos trabalhadores sociais usaram instrumentos para ERV; a ampla maioria os considerou relativamente úteis. Os instrumentos mais usados foram HCR-20, PCL-R e PCL-SV.Facultad de Ciencias Médica

Supervised nasal saline irrigations in otitis-prone children

Objectives: To retrospectively investigate the impact of supervised daily nasal saline irrigations (NSI) with 0. 9% saline solution in children with a history of recurrent acute otitis media (RAOM). Methods: A retrospective pilot study was planned to evaluate the possible effect of supervised NSI in reducing the number of acute otitis media (AOM) episodes in otitis-prone children aged 1-5 years, compared to children not instructed to correct NSI performance. Results: Analysis was based on the data contained in 173 charts (57.3% males, mean age of 30.9 \ub1 7.3 months). 52.0% of children had not been instructed to perform NSI, while the remaining (48.0%) patients had received supervised NSI. At the 4-months follow-up visit a significant reduced number of AOM episodes (1.03 \ub1 0.14 vs. 2.08 \ub1 0.16; p < 0.001) as well as antibiotic treatments (1.48 \ub1 0.17 vs. 2.59 \ub1 0.18; p < 0.001) was documented in children receiving supervised NSI compared to those not instructed for NSI performance. Conclusions: These data suggest that NSI should be considered in the therapeutic management of children with RAOM, and should be routinely prescribed as a daily adjunctive treatment to reduce acute infectious exacerbations in otitis-prone patients. Accurate parents training is crucial in order to improve children compliance and treatment effectiveness

Apelin-13 limits infarct size and improves cardiac postischemic mechanical recovery only if given after ischemia

We studied whether apelin-13 is cardioprotective against ischemia/reperfusion injury if given as either a pre- or postconditioning mimetic and whether the improved postischemic mechanical recovery induced by apelin-13 depends only on the reduced infarct size or also on a recovery of function of the viable myocardium. We also studied whether nitric oxide (NO) is involved in apelin-induced protection and whether the reported ischemia-induced overexpression of the apelin receptor (APJ) plays a role in cardioprotection. Langendorff-perfused rat hearts underwent 30 min of global ischemia and 120 min of reperfusion. Left ventricular pressure was recorded. Infarct size and lactate dehydrogenase release were determined to evaluate the severity of myocardial injury. Apelin-13 was infused at 0.5 \u3bcM concentration for 20 min either before ischemia or in early reperfusion, without and with NO synthase inhibition by N(G)-nitro-l-arginine (l-NNA). In additional experiments, before ischemia also 1 \u3bcM apelin-13 was tested. APJ protein level was measured before and after ischemia. Whereas before ischemia apelin-13 (0.5 and 1.0 \u3bcM) was ineffective, after ischemia it reduced infarct size from 54 \ub1 2% to 26 \ub1 4% of risk area (P < 0.001) and limited the postischemic myocardial contracture (P < 0.001). l-NNA alone increased postischemic myocardial contracture. This increase was attenuated by apelin-13, which, however, was unable to reduce infarct size. Ischemia increased APJ protein level after 15-min perfusion, i.e., after most of reperfusion injury has occurred. Apelin-13 protects the heart only if given after ischemia. In this protection NO plays an important role. Apelin-13 efficiency as postconditioning mimetic cannot be explained by the increased APJ level

Phenotype Profiling and Allergy in Otitis-Prone Children

Background: Otitis-prone children can present some distinctive clinical patterns and although a number of known risk factors for recurrent acute otitis media (RAOM) are known, no dedicated epidemiological models have been developed to explain clinical heterogeneity. Methods: A preliminary retrospective pilot study was planned to evaluate the possible effect of allergic disease in the development of different disease phenotypes in otitis-prone children aged 3-10 years, particularly the absence (simple RAOM), or presence of episodes of otitis media with effusion between acute infections (RAOM with OME). Results: Analysis was based on the data contained in 153 charts (55.6% males, mean age of 59.4 \ub1 16.4 months). 75.8% of children had a simple RAOM and 24.2% a RAOM with OME. Atopy or allergy were documented in respectively 47.7 and 41.3% of children considered as a whole. The prevalence of atopy or allergy was significantly higher in the children with a RAOM with OME (atopy: 73.0 vs. 39.5%, p < 0.001; allergy: 60.0 vs. 36.1%, p = 0.049), who also more frequently showed adenoidal hypertrophy (p = 0.016), chronic adenoiditis (p = 0.007), conductive hearing loss (p = 0.004), and impaired tympanometry (p < 0.001). Conclusions: These data suggest that children with a RAOM with OME are clinically different from children with simple RAOM, as they have a more complex clinical presentation that includes not only adenoidal disease and audiological impairment, but also an underlying allergy or atopy. The possibility that the factors mentioned above may be differently involved in the heterogeneous clinical manifestations occurring in otitis-prone children needs to be further investigated in ad hoc epidemiological studies

Evaluación de riesgo de incidentes agresivos y antisociales en población psiquiátrico forense brasileña

La evaluación del riesgo y el adecuado dimensionamiento de las conductas agresivas y comportamientos antisociales en un hospital psiquiátrico forense es necesaria para evitar prejuicios y para planificar las acciones preventivas correspondientes. El sesgo que producen el comportamiento agresivo y el antisocial puede tener efectos perjudiciales a la hora de planificar la externación. Sin embargo, no existía hasta este estudio información científica sobre la incidencia de estas conductas en hospitales psiquiátrico forenses brasileños.Facultad de Ciencias Médica

Evaluación de riesgo de incidentes agresivos y antisociales en población psiquiátrico forense brasileña

La evaluación del riesgo y el adecuado dimensionamiento de las conductas agresivas y comportamientos antisociales en un hospital psiquiátrico forense es necesaria para evitar prejuicios y para planificar las acciones preventivas correspondientes. El sesgo que producen el comportamiento agresivo y el antisocial puede tener efectos perjudiciales a la hora de planificar la externación. Sin embargo, no existía hasta este estudio información científica sobre la incidencia de estas conductas en hospitales psiquiátrico forenses brasileños.Facultad de Ciencias Médica

Evaluación de riesgo de incidentes agresivos y antisociales en población psiquiátrico forense brasileña

La evaluación del riesgo y el adecuado dimensionamiento de las conductas agresivas y comportamientos antisociales en un hospital psiquiátrico forense es necesaria para evitar prejuicios y para planificar las acciones preventivas correspondientes. El sesgo que producen el comportamiento agresivo y el antisocial puede tener efectos perjudiciales a la hora de planificar la externación. Sin embargo, no existía hasta este estudio información científica sobre la incidencia de estas conductas en hospitales psiquiátrico forenses brasileños.Facultad de Ciencias Médica