Zwischen Ver-hüllung und Ent-hüllung

Ihren Ursprung fand vorliegende Arbeit in der Aussage Michel Foucaults, Sexualität habe im 18. Jahrhunderts eine diskursive Explosion erfahren. Es galt zu untersuchen, inwiefern diese Entwicklung auch in der Literatur festzustellen sei. Im ersten Teil der Diplomarbeit werden sozialgeschichtliche Umstände beleuchtet, Sexualität etwa im medizinischen Kontext untersucht. Dabei geht es vor allem um die Beschreibung einer sich entfaltenden Form der bürgerlichen Sexualität: Ein erstarkendes Bürgertum, das sich von der Aristokratie und dem einfachen Volk abzugrenzen versucht, entwickelt sein eigenes Konzept der Liebe und der Sexualität. Vor allem dient dieser erste Teil der Arbeit auch der Präsentation zweier ideengeschichtlicher Entwürfe, die als gegensätzliche Pole die Darstellung von Sexualität beeinflussen werden: Einerseits der französische Materialismus, der einer libertinären Lebensphilosophie Vorschub leistet, andererseits die Bewegung der Empfindsamkeit, die durch ihren Freundschaftskult eine Desexualisierung zur Folge hat. Vor dem Hintergrund dieses Spannungsfeldes soll im zweiten Teil die Analyse von literarischen Werken erfolgen: Als Untersuchungsgegenstand werden primär bürgerliche Romane aus Frankreich, England und Deutschland herangezogen. Dabei wird auf zwei Ebenen gearbeitet: Erstens wird die sprachliche Darstellung betrachtet. Wie bereits der Titel vorliegender Diplomarbeit verrät, wird die Darstellbarkeit analysiert. Es wird die Frage gestellt, bis zu welchem Grad sich eine Benennung des Geschlechtlichen mit dem Begriff der Tugendhaftigkeit als vereinbar erweist. Auf zweiter Ebene werden bürgerliche Motive untersucht, besonders in Hinblick auf deren Repräsentation eines genuin bürgerlichen Sexualkonzepts. Das Ergebnis der Textanalysen ist schließlich die Bestätigung der Ansicht Foucaults: Sexualität scheint im Roman des 18. Jahrhunderts allgegenwärtig. Die Wiedergabe einer entsexualisierten Welt erweist sich als unmöglich, da selbst die Tugendhaftigkeit ihre Unschuld verloren hat (Richardson, Gellert). Das Wissen um die Sexualität ist bereits zu sehr ausgebildet, als dass eine tugendhafte Reinheit unhinterfragt bleiben kann. Dieses Wissen spiegelt sich auch auf lexikaler Ebene: Das 18. Jahrhundert ist das Jahrhundert des double entendre. Autoren wie Laurence Sterne, Denis Diderot oder Henry Fielding arbeiten mit Doppeldeutigkeiten und dehnen somit den bürgerlichen Moralkodex der Wohlanständigkeit. Eine dichotome Gegenüberstellung von sexuell impliziter und sexuell expliziter Darstellung erweist sich als irrelevant: Während der pornographische Roman eines John Cleland durch seine exzessive Metaphorik den Blick verstellt, verweisen die Romane Wielands oder auch Richardsons durch geschickte Aussparungen auf einen sexuellen Sachverhalt und spielen mit der Macht der Einbildungskraft. Dadurch sind sie wohl nicht unschuldiger als der sexuell explizite Roman

Urban Heat Islands Strategy Plan Vienna – Implementing Urban Green Infrastructure to Reduce Negative Effects of Urban Heat Islands

Urban heat islands (UHI) have been known since the 19th century (Howard, 1820) and describe the difference in temperature between cities and their rural surroundings. This difference can be up to 12°C (Eliasson, 2000, 31); the phenomenon is caused by the transformation of natural surfaces through e.g. soil sealing, construction of infrastructure and buildings. However, differences in temperature not only occur between cities and their adjacent areas, but also within different parts of cities depending on the provision of green and blue infrastructure as well as on their share of sealed surfaces. The situation is further aggravated by a changing climate. Numerous studies state that the number of heat days (maximum temperature of at least 30°C) as well as the number and duration of heat waves will increase worldwide – especially in cities due to their sealed surfaces, building density and lack of green space (Formayer et al., 2008; Bowler et al., 2010). This problem will become even more crucial in the future: in 2005 approx. half of the world population lived in urban regions and this number is predicted to rise by up to nearly two thirds by 2050 (Schlünzen, 2012). Consequences of those growing cities and expanding urban areas are further densification of settlement areas and loss of open and green space; this strengthens the urban heat islands effect even more. Urban heat islands can have negative effects on human health and wellbeing with sensitive groups such as the elderly being especially affected (Allex et al., 2013). To counteract UHI and their negative effects, the “Urban Heat Islands Strategy Plan Vienna” has been elaborated as part of a Central Europe project (www.http://eu-uhi.eu/, 2011-2014). The aim was the development of a strategy for the City of Vienna to implement open space planning as well as urban ecology measures to reduce the negative aspects of UHI. Within the project, a guideline has been elaborated by the project team and members of the Environmental Protection Department Vienna (MA 22) to support planners, architects as well as members of the Vienna City Administration, to show possibilities of technical and strategic measures against UHI as well as their potential to reduce urban heat, and to point out planning tools and planning levels. The guideline is available online (https://www.wien.gv.at/umweltschutz/raum/uhi-strategieplan.html, in German)

Urban green infrastructure planning as a contribution to the smart ‘green’ city

The urban green infrastructure is getting due to the strong growth of the City of Vienna under increasing pressure. A foresighted planning of green and open spaces is necessary to obtain the different "Ecosytem Services" - provision-related services, regulatory services, cultural services and support services (MEA 2005). Additionally an increase in the number of hot days and thus an increase of the heat load in the city is predicted for Vienna (ZAMG 2012). Again, making a foresighted planning of green and open spaces is a significant contribution to meet these climatic challenges (Kuffner A. 2012, Hagen et al. 2010). Based on the concept of "green infrastructure" (Pauleit et al. 2011) and the ecosystem services of these, it is shown which contribution - in particular to reduce the heating of the city - they can make to the Smart City concept

Time and phenotype-dependent transcriptome analysis in AAV-TGFβ1 and Bleomycin-induced lung fibrosis models

We have previously established a novel mouse model of lung fibrosis based on Adeno-associated virus (AAV)-mediated pulmonary overexpression of TGFβ1. Here, we provide an in-depth characterization of phenotypic and transcriptomic changes (mRNA and miRNA) in a head-to-head comparison with Bleomycin-induced lung injury over a 4-week disease course. The analyses delineate the temporal state of model-specific and commonly altered pathways, thereby providing detailed insights into the processes underlying disease development. They further guide appropriate model selection as well as interventional study design. Overall, Bleomycin-induced fibrosis resembles a biphasic process of acute inflammation and subsequent transition into fibrosis (with partial resolution), whereas the TGFβ1-driven model is characterized by pronounced and persistent fibrosis with concomitant inflammation and an equally complex disease phenotype as observed upon Bleomycin instillation. Finally, based on an integrative approach combining lung function data, mRNA/miRNA profiles, their correlation and miRNA target predictions, we identify putative drug targets and miRNAs to be explored as therapeutic candidates for fibrotic diseases. Taken together, we provide a comprehensive analysis and rich data resource based on RNA-sequencing, along with a strategy for transcriptome-phenotype coupling. The results will be of value for TGFβ research, drug discovery and biomarker identification in progressive fibrosing interstitial lung diseases

Sustainable Management of Urban Green Infrastructure – The Challenge of Providing High-Quality Green in Multi-Storey Residential Construction

Vienna is known as one of the most liveable cities worldwide (Mercer, 2015), not least because of Vienna’s green infrastructure (GI). These qualities of life and the trend of urbanisation lead to strong population growth in Vienna. It is predicted that the Viennese population will grow from 1.8 million (2015) to 2 million in 2029 (MA 23, 2014); to offer living space, the creation of up to 120,000 new homes is planned until 2050 (MA 18, 2014). The growth and the resulting exploitation pressure on the (green) areas pose a major challenge for the City of Vienna. The loss of green space induced by land use results in the reduction or loss of ecosystem services. The negative effects of the decline of green areas and the increasing soil sealing already occur especially in areas of high population density. Furthermore, increasing heat stress and risks related to natural disasters like the flood event in 2002 show the importance of green space in urban areas for the maintenance of ecosystem functions. Therefore, a challenge of the next years will be to maintain a high-quality and efficient network of GI. At the moment, the floor area ratio (“Geschoßflächenzahl”) and other values like the degree of soil sealing, the density rate for buildings, building heights etc. are the defining parameters for urban development in Vienna and regulate the degree of building coverage. The supply of the neighbourhoods with open/green space is determined only indirectly. Besides, those parameters are not able to state the quality of green space for humans. Vienna has already recognised the importance of GI and develops guides for developers and urban planners to contribute to encourage GI in the city (MA 18, 2014; MA 22, 2013; MA 22, 2015). But a clear framework for the conservation and provision of minimum standards for urban green space, however, is still missing; incentives for implementation of GI elements are primarily given through grants. Some cities have developed defining parameters or policy instruments for GI to enable a management of open space supply and quality (e.g. Berlin: “Biotope Area Factor”; Malmö: “Green Space Factor”; Seattle: “Green Factor”, Helsinki...), but in the current practice of applying the green space factors almost only ecological aspects are taken into account (Kruuse, 2011; Szulczewska et al., 2014). Socio-cultural aspects such as usability, aesthetics or recreation are rarely considered. For urban planning and administration it would be important to start thinking about a green space factor which, beneath size and space consumption, also takes into account socio-cultural aspects. The main objective of the “AddedValueGreen!” project was to develop a green and open space factor (“Grünflächenfaktor” or “GFF”) which encompasses regulating, socio-cultural and economic effects of urban GI (uGI). The focus was on the management and intervention of private and housing-related spaces to secure a certain amount of high-quality open/green space on building lots. Through the evaluation of housing projects by using the GFF, deficits in the green space supply and quality can be identified and recommendations to improve the GI can be derived. Furthermore, it will be possible to integrate this evaluation tool into other planning levels or management tools

A laboratory pilot study on voids in flowable bulk-fill composite restorations in bovine Class-II and endodontic access cavities after sonic vibration

This pilot study investigated whether sonic-powered application of a bulk-fill resin-based composite (RBC) in Class-II or endodontic access cavities reduces void formation. The crowns and roots of 60 bovine teeth with Class-II cavities (C) and endodontic access cavities (E) respectively, were assigned to ten groups (C1–C5, E1–E5). Cavities were filled with RBC (SDR flow + , one increment) using different application techniques: no adaptation (C1 + E1), spreading of RBC on the cavity surfaces with a dental explorer tip (C2 + E2), low (C3 + E3) or high frequency (C4 + E4) direct activation by inserting a sonic-powered tip into RBC and high frequency indirect activation with an ultrasonic insertion tip (C5 + E5). The restorations were light-cured and investigated for voids using microtomography. The number of voids and percentage of voids related to the volume were statistically analysed (α  0.05). The percentage of voids showed no differences in E1-E5 (p > 0.05). C4 showed a significantly higher percentage of voids compared to C2 (p < 0.001). There is no benefit in applying sonic vibration when filling Class-II or endodontic access cavities

Inducible stem cell-derived embryos capture mouse morphogenetic events in vitro

The development of mouse embryos can be partially recapitulated by combining embryonic (ES), trophoblast (TS) and extra-embryonic endoderm (XEN) stem cells to generate ETX-embryos. Although ETX-embryos transcriptionally capture the mouse gastrula, their ability to recapitulate complex morphogenic events such as gastrulation is limited, possibly due to the limited potential of XEN cells. To address this, we generated ES cells transiently expressing transcription factor Gata4 that drives the extra-embryonic endoderm fate and combined them together with ES cells and TS cells to generate induced ETX-embryos (iETX-embryos). We show that iETX-embryos establish a robust anterior signalling centre that migrates unilaterally to break embryo symmetry. Furthermore, iETX-embryos gastrulate generating embryonic and extra-embryonic mesoderm, and definitive endoderm. Our findings reveal that replacement of XEN cells with ES cells transiently expressing Gata4 endows iETX-embryos with greater developmental potential, thus enabling the study of the establishment of anterior-posterior patterning and gastrulation in an in vitro system.This work was supported by a European Research Council Grant (RG77946)Wellcome Trust (207415/Z/17/Z), Open Philanthropy, Shurl and Kay Curci, and Weston Havens Foundations grants awarded to M.Z.G.; K.Y.C.L. is supported by the Croucher Foundation and Cambridge Trust. F.H. is supported by a European Research Council Grant (695669) and Wellcome Trust (WT108438/C/15/Z). J.D.J. is supported by the Biotechnology and Biological Sciences Research Council

Inducible stem cell-derived embryos capture mouse morphogenetic events in vitro

The development of mouse embryos can be partially recapitulated by combining embryonic (ES), trophoblast (TS) and extra-embryonic endoderm (XEN) stem cells to generate ETX-embryos. Although ETX-embryos transcriptionally capture the mouse gastrula, their ability to recapitulate complex morphogenic events such as gastrulation is limited, possibly due to the limited potential of XEN cells. To address this, we generated ES cells transiently expressing transcription factor Gata4 that drives the extra-embryonic endoderm fate and combined them together with ES cells and TS cells to generate induced ETX-embryos (iETX-embryos). We show that iETX-embryos establish a robust anterior signalling centre that migrates unilaterally to break embryo symmetry. Furthermore, iETX-embryos gastrulate generating embryonic and extra-embryonic mesoderm, and definitive endoderm. Our findings reveal that replacement of XEN cells with ES cells transiently expressing Gata4 endows iETX-embryos with greater developmental potential, thus enabling the study of the establishment of anterior-posterior patterning and gastrulation in an in vitro system.This work was supported by a European Research Council Grant (RG77946)Wellcome Trust (207415/Z/17/Z), Open Philanthropy, Shurl and Kay Curci, and Weston Havens Foundations grants awarded to M.Z.G.; K.Y.C.L. is supported by the Croucher Foundation and Cambridge Trust. F.H. is supported by a European Research Council Grant (695669) and Wellcome Trust (WT108438/C/15/Z). J.D.J. is supported by the Biotechnology and Biological Sciences Research Council

Phenocopy – A Strategy to Qualify Chemical Compounds during Hit-to-Lead and/or Lead Optimization

A phenocopy is defined as an environmentally induced phenotype of one individual which is identical to the genotype-determined phenotype of another individual. The phenocopy phenomenon has been translated to the drug discovery process as phenotypes produced by the treatment of biological systems with new chemical entities (NCE) may resemble environmentally induced phenotypic modifications. Various new chemical entities exerting inhibition of the kinase activity of Transforming Growth Factor β Receptor I (TGF-βR1) were qualified by high-throughput RNA expression profiling. This chemical genomics approach resulted in a precise time-dependent insight to the TGF-β biology and allowed furthermore a comprehensive analysis of each NCE's off-target effects. The evaluation of off-target effects by the phenocopy approach allows a more accurate and integrated view on optimized compounds, supplementing classical biological evaluation parameters such as potency and selectivity. It has therefore the potential to become a novel method for ranking compounds during various drug discovery phases