Secondary circulation in natural streams

Secondary circulation which is sometimes referred to as secondary flow, secondary current or transverse current is an important phenomenon in natural streams and plays an important role in many natural processes in streams such as stream channel meander, bank erosion, bed scour, resuspension, and movement of sediment. Secondary circulation is that component of flow which is not in the main flow direction and is small as compared to the main flow velocity. A computerized data collection system for secondary circulation data acquisition in natural streams was developed and utilized in the field. The system includes an electromagnetic current meter, a micro-computer, an interface between the computer and the current meter, and a specially designed supporting structure. Secondary circulation data was collected in the Sangamon River near Mahomet, Illinois, utilizing the data collection system. A mathematical model for secondary circulation based on an existing model has been developed and tested against the data collected in the field. Model results generally reproduce similar secondary circulation patterns as observed from the field data but over-estimate the magnitudes of the velocities.U.S. Department of the InteriorU.S. Geological SurveyOpe

Cryo-EM structures of tau filaments from Alzheimer's disease

Alzheimer's disease is the most common neurodegenerative disease, and there are no mechanism-based therapies. The disease is defined by the presence of abundant neurofibrillary lesions and neuritic plaques in the cerebral cortex. Neurofibrillary lesions comprise paired helical and straight tau filaments, whereas tau filaments with different morphologies characterize other neurodegenerative diseases. No high-resolution structures of tau filaments are available. Here we present cryo-electron microscopy (cryo-EM) maps at 3.4-3.5 Å resolution and corresponding atomic models of paired helical and straight filaments from the brain of an individual with Alzheimer's disease. Filament cores are made of two identical protofilaments comprising residues 306-378 of tau protein, which adopt a combined cross-β/β-helix structure and define the seed for tau aggregation. Paired helical and straight filaments differ in their inter-protofilament packing, showing that they are ultrastructural polymorphs. These findings demonstrate that cryo-EM allows atomic characterization of amyloid filaments from patient-derived material, and pave the way for investigation of a range of neurodegenerative diseases