593 research outputs found
Cotton harvest aids (1994)
Removing leaves and opening bolls with harvest aids are important components of a management plan for producing high-quality cotton fiber. When not properly managed, harvest aids may reduce yield and quality. By properly managing the time of defoliation, the crop can be prepared for a timely harvest, which is critical in Missouri's short season environment. The number of suitable hours for harvest operations decreases rapidly during the fall. With timely harvest, earlier stalk destruction can occur as an essential part of insect and disease management. The objective of this publication is to provide information on proper defoliation techniques and the available harvest aids on the market today.David W. Albers (State Extension Specialist-Cotton, Delta Center), Fred Fishel (Pesticide Application Trainer Supervisor), Jill B. Mobley (Research Specialist).New March 199
Mass spectrometric gas composition measurements associated with jet interaction tests in a high-enthalpy wind tunnel
Knowledge of test gas composition is important in wind-tunnel experiments measuring aerothermodynamic interactions. This paper describes measurements made by sampling the top of the test section during runs of the Langley 7-Inch High-Temperature Tunnel. The tests were conducted to determine the mixing of gas injected from a flat-plate model into a combustion-heated hypervelocity test stream and to monitor the CO2 produced in the combustion. The Mass Spectrometric (MS) measurements yield the mole fraction of N2 or He and CO2 reaching the sample inlets. The data obtained for several tunnel run conditions are related to the pressures measured in the tunnel test section and at the MS ionizer inlet. The apparent distributions of injected gas species and tunnel gas (CO2) are discussed relative to the sampling techniques. The measurements provided significant real-time data for the distribution of injected gases in the test section. The jet N2 diffused readily from the test stream, but the jet He was mostly entrained. The amounts of CO2 and Ar diffusing upward in the test section for several run conditions indicated the variability of the combustion-gas test-stream composition
Healthcare Utilization and Quality of Life Improvement after Ablation for Paroxysmal AF in Younger and Older Patients
Background
Atrial fibrillation (AF) prevalence increases significantly with age. Little is known about the effect of AF ablation on quality of life and healthcare utilization in the elderly. The objective of this study was to quantify the healthcare utilization and quality of life benefits of catheter ablation for AF, for patients ≥65 years compared to patients <65 years.
Methods
Two multicenter U.S. registry studies enrolled patients with paroxysmal AF. Baseline characteristics and acute outcomes were collected for 736 patients receiving catheter ablation with the Navistar® ThermoCool® SF Catheter (Biosense Webster, Inc., Diamond Bar, CA, USA). Healthcare utilization and quality of life outcomes were collected through 1 year postablation for 508 patients.
Results
The rates of acute pulmonary vein isolation were high and similar between patients ≥65 years and <65 years (97.5% vs 95.8%, P = 0.2130). Length of stay for the index procedure was similar between age groups with 82.2% of the older group and 83.2% of the younger group having one-day hospitalization. Disease-specific quality of life instrument scores improved significantly and similarly for older and younger patients at 1 year postablation, compared to baseline. AF-related hospitalizations and emergency department visits were similar or lower in older patients compared to younger patients, as reported at 1 year postablation.
Conclusion
For older patients undergoing catheter ablation for paroxysmal AF, healthcare utilization parameters were lower or not significantly different than for younger patients, and quality of life outcomes were similarly improved. These findings support the use of catheter ablation as a treatment option in older patients with paroxysmal AF
Delivery of a chromosomally normal child from an oocyte with reciprocal aneuploid polar bodies
High Polymer Content 3,5-Pyridine- Polybenzimidazole Copolymer Membranes with Improved Compressive Properties
Phosphorylation of histone H3(T118) alters nucleosome dynamics and remodeling
Nucleosomes, the fundamental units of chromatin structure, are regulators and barriers to transcription, replication and repair. Post-translational modifications (PTMs) of the histone proteins within nucleosomes regulate these DNA processes. Histone H3(T118) is a site of phosphorylation [H3(T118ph)] and is implicated in regulation of transcription and DNA repair. We prepared H3(T118ph) by expressed protein ligation and determined its influence on nucleosome dynamics. We find H3(T118ph) reduces DNA–histone binding by 2 kcal/mol, increases nucleosome mobility by 28-fold and increases DNA accessibility near the dyad region by 6-fold. Moreover, H3(T118ph) increases the rate of hMSH2–hMSH6 nucleosome disassembly and enables nucleosome disassembly by the SWI/SNF chromatin remodeler. These studies suggest that H3(T118ph) directly enhances and may reprogram chromatin remodeling reactions
Derivation of 30 human embryonic stem cell lines—improving the quality
We have derived 30 human embryonic stem cell lines from supernumerary blastocysts in our laboratory. During the derivation process, we have studied new and safe method to establish good quality lines. All our human embryonic stem cell lines have been derived using human foreskin fibroblasts as feeder cells. The 26 more recent lines were derived in a medium containing serum replacement instead of fetal calf serum. Mechanical isolation of the inner cell mass using flexible metal needles was used in deriving the 10 latest lines. The lines are karyotypically normal, but culture adaptation in two lines has been observed. Our human embryonic stem cell lines are banked, and they are available for researchers
The Vehicle, Spring 1981
Vol. 22, No. 2
Table of Contents
Old Farmers at the Arcade CafeJohn Stockmanpage 4
ConfettiCathy Georgepage 6
Ode to a Corned Beef SandwichJeff Bennettpage 6
The Ice on Kirschner\u27s CreekScott Fishelpage 7
Love Poem to LindaJohn Stockmanpage 7
Grandfather\u27s PortraitJames Marshpage 8
The MassageKathleen Alakspage 9
A Driving ForceSandy Youngpage 10
King DandelionNancy Siebenpage 12
One Afternoon - Contemplating HouseworkKelli Sanderpage 13
Tent WallsAndy Sudkamppage 14
The SentinelElise Hempelpage 16
Daddy\u27s AftershaveJeff Bennettpage 16
The WeddingChris Goerlichpage 17
UntitledCarol Hansenpage 17
Treasures in the YardScott Fishelpage 18
Hitchhiker\u27s BootsAndy Sudkamppage 20
The RaffleLaura Henrypage 21
A Walk at NightJudi Jinespage 24
Morning in the DumpJeff Bennettpage 24
In Praise of Chocolate Ice CreamJohn Stockmanpage 25
Summer on the Isle of PalmsElisabeth Cristpage 26
The WaveHerbert S. Demminpage 27
RememberingJohn Kleinsteiberpage 27
PotatoJohn Stockmanpage 28
Late ShowChris Goerlichpage 30
Love in Him - JoeDebbie Klinnertpage 31
ShoeScott Fishelpage 35
The DrinkerBob Huntpage 36
The WidowGeorge Ndu Igbudupage 37
ElectricityScott Fishelpage 37
Hatchet JackB.L. Davidsonpage 39
Walking Home LateJohn Stockmanpage 41
NovemberCindy Hubbarttpage 41
On the BusLaura Henrypage 42
HaikuJames Marshpage 43
SpillwayGloria Rhoadspage 43
Art
Cover design by Linda Fraembs
PhotographRobin Scholzpage 3
PhotographRobin Scholzpage 5
PhotographMichelle Glassmeyerpage 15
PhotographRobert Schinaglpage 19
PhotographTom Robertspage 38
PhotographRobert Schinaglpage 44https://thekeep.eiu.edu/vehicle/1039/thumbnail.jp
Analysis of Microsatellite Polymorphism in Inbred Knockout Mice
Previously, we found that the genotype of 42 out of 198 mouse microsatellite loci, which are distributed among all chromosomes except the Y chromosome, changed from monomorphism to polymorphism (CMP) in a genetically modified inbred mouse strain. In this study, we further examined whether CMP also relates to the homologous recombination in gene knockout (KO) mouse strains. The same 42 microsatellite loci were analyzed by polymerase chain reaction (PCR) in 29 KO inbred mouse strains via short tandem sequence repeat (STR) scanning and direct sequence cloning to justify microsatellite polymorphisms. The C57BL/6J and 129 mouse strains, from which these 29 KO mice were derived, were chosen as the background controls. The results indicated that 10 out of 42 (23.8%) loci showed CMP in some of these mouse strains. Except for the trinucleotide repeat locus of D3Mit22, which had microsatellite CMP in strain number 9, the core sequences of the remaining 41 loci were dinucleotide repeats, and 9 out of 41 (21.95%) showed CMPs among detected mouse strains. However, 11 out of 29 (37.9%) KO mice strains were recognized as having CMPs. The popular dinucleotide motifs in CMP were (TG)n (50%, 2/4), followed by (GT)n (27.27%, 3/11) and (CA)n (23.08%, 3/13). The microsatellite CMP in (CT)n and (AG)n repeats were 20% (1/5). According to cloning sequencing results, 6 KO mouse strains showed insertions of nucleotides whereas 1 showed a deletion. Furthermore, 2 loci (D13Mit3 and D14Mit102) revealed CMP in 2 strains, and mouse strain number 9 showed CMPs in two loci (D3Mit22 and D13Mit3) simultaneously. Collectively, these results indicated that microsatellite polymorphisms were present in the examined inbred KO mice
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