Característica do estilo individual de Vasily Vasilyevich Rozanov

The present article analyzes the individual style of the famous Russian philosopher, writer, prose writer, and journalist, Vasily Vasilyevich Rozanov. It is proved that the writer's idiostyle is unique in Russian literature of the late 19th and early 20th centuries. The formation of an individual style took place in three stages. At the first stage, the philosophical treatise “On Understanding” was created, in which the categories of knowledge, wisdom, etc. were subjected to philosophical analysis. At the second stage, Rozanov’s individual style, the critic, was formed. In this period, the individual style is characterized by inconsistency. At the third stage, Rozanov’s individual style is formed, a prose writer, the key characteristics of whose style were unsystematic, contradictory and antinomic.El presente artículo describe el estilo individual de la famosa rusa, escritor, prose escritor, y periodista, Vasily Vasilyevich Rozanov. Se trata de que el escritor de los idiostyle es único en los ríos de los Estados Unidos y de los últimos 20 años. La formación de un estilo individual tuvo lugar en tres etapas. En la primera etapa, se creó la filosofía táctica "On Understanding" en las categorías de conocimiento, sabiduría, etc. se han inscrito en el análisis filosófico. En la segunda etapa, Rozanov el estilo individual, la crítica, fue formada. En este período, el estilo individual se considera inconsistencia. En la tercera etapa, Rozanov's individual estilo se ha formado, la prose de escritor, la clave de su estilo han sido unsystematic, contradictoria y antinomic.O presente artigo analisa o estilo individual do famoso filósofo, escritor, escritor de prosa e jornalista russo Vasily Vasilyevich Rozanov. Está provado que o idiostilo do escritor é único na literatura russa do final do século XIX e início do século XX. A formação de um estilo individual ocorreu em três etapas. No primeiro estágio, foi criado o tratado filosófico "On Understanding", no qual as categorias de conhecimento, sabedoria, etc. foram submetidos à análise filosófica. Na segunda etapa, o estilo individual de Rozanov, o crítico, foi formado. Nesse período, o estilo individual é caracterizado por inconsistência. No terceiro estágio, formou-se o estilo individual de Rozanov, um escritor de prosa cujas característicaschave de cujo estilo eram não sistemáticas, contraditórias e antinômicas

Arquetipos en la estructura del metaconcepto

The history of studying the cognitive structure of our consciousness and its minimal mental unit dates back to the beginning of the 20th century and is associated with the name of the Russian scientist S.А. Askoldov, the scientific legacy of whom was not actually forgotten during the Soviet period. Appealing to the methods of representation of knowledge about the world has aroused the interest in the concept category. The basics which reflect primary human values are metaconcept. The present paper proves that the basis of the structure of the meta concept is an archetype.La historia del estudio de la estructura cognitiva de nuestra conciencia y su unidad mental mínima se remonta a principios del siglo XX y está asociada con el nombre del científico ruso S.А. Askoldov, cuyo legado científico no fue olvidado en realidad durante el período soviético. Apelar a los métodos de representación del conocimiento sobre el mundo ha despertado el interés en la categoría de concepto. Los conceptos básicos que reflejan los valores humanos primarios son el metaconcepto. El presente trabajo demuestra que la base de la estructura del metaconcepto es un arquetipo

Genomic features of resistant <i>Klebsiella pneumonia</i>, isolated from the bloodstream and cerebrospinal fluid of pediatric hospital patients

Introduction. Carbapenemase-producing Klebsiella pneumoniae (CP-Kp), which are international high-risk clones, have become a problem of utmost importance. CP-Kps, adapting to the hospital environment, evolve into convergent pathotypes. Such variants combine traits of two genetic lineages: multidrug resistant (MDR) and hypervirulent. The pathotypes, along with MDR K. pneumoniae, pose an exceptional threat to young patients during systemic infection. The objective of this study is the detailed molecular genetic analysis of MDR isolates of K. pneumoniae detected during the monitoring of resistant Gram-negative bacteria at the National Medical Research Center for Children’s Health in 2014–2021. Materials and methods. Whole-genome sequencing with a subsequent bioinformatics analysis of eight MDR isolates from the bloodstream and cerebrospinal fluid. Results. MDR isolates belonged to 4 sublineages (SL): SL307, SL395, SL29 and SL1198. In the genomes of 6 pangrug-resistant (PDR) isolates, genes associated with resistance to all categories of antibiotics recommended for Enterobacteriaceae therapy were identified. Plasmids were present in all genomes. In 6 isolates, plasmids contained heavy metal ion resistance operons in addition to antibiotic resistance genes. Prophages within the plasmids were also involved in the transfer of resistance genes. The ST395 isolate from the cerebrospinal fluid belonged to the convergent pathotype in terms of resistance and virulence. Comparison of genomes within SLs revealed recombination events in the K- and O-locus regions and the Yersiniabactin operon. Conclusion. Thus, in a sample of resistant K. pneumoniae isolated from bloodstream and cerebrospinal fluid, 6 PDR isolates were detected, one of which belongs to the convergent pathotype ST395

Derivatives of 9-phosphorylated acridine as butyrylcholinesterase inhibitors with antioxidant activity and the ability to inhibit β-amyloid self-aggregation: potential therapeutic agents for Alzheimer’s disease

We investigated the inhibitory activities of novel 9-phosphoryl-9,10-dihydroacridines and 9-phosphorylacridines against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and carboxylesterase (CES). We also studied the abilities of the new compounds to interfere with the self-aggregation of β-amyloid (Aβ42) in the thioflavin test as well as their antioxidant activities in the ABTS and FRAP assays. We used molecular docking, molecular dynamics simulations, and quantum-chemical calculations to explain experimental results. All new compounds weakly inhibited AChE and off-target CES. Dihydroacridines with aryl substituents in the phosphoryl moiety inhibited BChE; the most active were the dibenzyloxy derivative 1d and its diphenethyl bioisostere 1e (IC50 = 2.90 ± 0.23 µM and 3.22 ± 0.25 µM, respectively). Only one acridine, 2d, an analog of dihydroacridine, 1d, was an effective BChE inhibitor (IC50 = 6.90 ± 0.55 μM), consistent with docking results. Dihydroacridines inhibited Aβ42 self-aggregation; 1d and 1e were the most active (58.9% ± 4.7% and 46.9% ± 4.2%, respectively). All dihydroacridines 1 demonstrated high ABTS•+-scavenging and iron-reducing activities comparable to Trolox, but acridines 2 were almost inactive. Observed features were well explained by quantum-chemical calculations. ADMET parameters calculated for all compounds predicted favorable intestinal absorption, good blood–brain barrier permeability, and low cardiac toxicity. Overall, the best results were obtained for two dihydroacridine derivatives 1d and 1e with dibenzyloxy and diphenethyl substituents in the phosphoryl moiety. These compounds displayed high inhibition of BChE activity and Aβ42 self-aggregation, high antioxidant activity, and favorable predicted ADMET profiles. Therefore, we consider 1d and 1e as lead compounds for further in-depth studies as potential anti-AD preparations

Archetypes in metaconcept structure

The history of studying the cognitive structure of our consciousness and its minimal mental unit dates back to the beginning of the 20th century and is associated with the name of the Russian scientist S.А. Askoldov, the scientific legacy of whom was not actually forgotten during the Soviet period. Appealing to the methods of representation of knowledge about the world has aroused the interest in the concept category. The basics which reflect primary human values are metaconcept. The present paper proves that the basis of the structure of the meta concept is an archetype.La historia del estudio de la estructura cognitiva de nuestra conciencia y su unidad mental mínima se remonta a principios del siglo XX y está asociada con el nombre del científico ruso S.А. Askoldov, cuyo legado científico no fue olvidado en realidad durante el período soviético. Apelar a los métodos de representación del conocimiento sobre el mundo ha despertado el interés en la categoría de concepto. Los conceptos básicos que reflejan los valores humanos primarios son el metaconcepto. El presente trabajo demuestra que la base de la estructura del metaconcepto es un arquetipo

Arquetipos en la estructura del metaconcepto

The history of studying the cognitive structure of our consciousness and its minimal mental unit dates back to the beginning of the 20th century and is associated with the name of the Russian scientist S.А. Askoldov, the scientific legacy of whom was not actually forgotten during the Soviet period. Appealing to the methods of representation of knowledge about the world has aroused the interest in the concept category. The basics which reflect primary human values are metaconcept. The present paper proves that the basis of the structure of the meta concept is an archetype.La historia del estudio de la estructura cognitiva de nuestra conciencia y su unidad mental mínima se remonta a principios del siglo XX y está asociada con el nombre del científico ruso S.А. Askoldov, cuyo legado científico no fue olvidado en realidad durante el período soviético. Apelar a los métodos de representación del conocimiento sobre el mundo ha despertado el interés en la categoría de concepto. Los conceptos básicos que reflejan los valores humanos primarios son el metaconcepto. El presente trabajo demuestra que la base de la estructura del metaconcepto es un arquetipo

Archetypes in Metaconcept Structure

The history of studying the cognitive structure of our consciousness and its minimal mental unit dates back to the beginning of the 20th century and is associated with the name of the Russian scientist S.А. Askoldov, the scientific legacy of whom was not actually forgotten during the Soviet period. Appealing to the methods of representation of knowledge about the world has aroused the interest in the concept category. The basics which reflect primary human values are metaconcept. The present paper proves that the basis of the structure of the meta concept is an archetype

Signatures of Dermal Fibroblasts from RDEB Pediatric Patients

The recessive form of dystrophic epidermolysis bullosa (RDEB) is a debilitating disease caused by impairments in the junctions of the dermis and the basement membrane of the epidermis. Mutations in the COL7A1 gene induce multiple abnormalities, including chronic inflammation and profibrotic changes in the skin. However, the correlations between the specific mutations in COL7A1 and their phenotypic output remain largely unexplored. The mutations in the COL7A1 gene, described here, were found in the DEB register. Among them, two homozygous mutations and two cases of compound heterozygous mutations were identified. We created the panel of primary patient-specific RDEB fibroblast lines (FEB) and compared it with control fibroblasts from healthy donors (FHC). The set of morphological features and the contraction capacity of the cells distinguished FEB from FHC. We also report the relationships between the mutations and several phenotypic traits of the FEB. Based on the analysis of the available RNA-seq data of RDEB fibroblasts, we performed an RT-qPCR gene expression analysis of our cell lines, confirming the differential status of multiple genes while uncovering the new ones. We anticipate that our panels of cell lines will be useful not only for studying RDEB signatures but also for investigating the overall mechanisms involved in disease progression

Signatures of Dermal Fibroblasts from RDEB Pediatric Patients

The recessive form of dystrophic epidermolysis bullosa (RDEB) is a debilitating disease caused by impairments in the junctions of the dermis and the basement membrane of the epidermis. Mutations in the COL7A1 gene induce multiple abnormalities, including chronic inflammation and profibrotic changes in the skin. However, the correlations between the specific mutations in COL7A1 and their phenotypic output remain largely unexplored. The mutations in the COL7A1 gene, described here, were found in the DEB register. Among them, two homozygous mutations and two cases of compound heterozygous mutations were identified. We created the panel of primary patient-specific RDEB fibroblast lines (FEB) and compared it with control fibroblasts from healthy donors (FHC). The set of morphological features and the contraction capacity of the cells distinguished FEB from FHC. We also report the relationships between the mutations and several phenotypic traits of the FEB. Based on the analysis of the available RNA-seq data of RDEB fibroblasts, we performed an RT-qPCR gene expression analysis of our cell lines, confirming the differential status of multiple genes while uncovering the new ones. We anticipate that our panels of cell lines will be useful not only for studying RDEB signatures but also for investigating the overall mechanisms involved in disease progression

Clinical Features and Management of the Disease Caused by New Coronaviral Infection (COVID-19) in Children. Version 2

The Ministry of Health of the Russian Federation jointly with professional association and experts in the field of pediatrics, infectious diseases and resuscitation has revised guidelines “Clinical Features and Management of the Disease Caused by New Coronaviral Infection (COVID-19) in Children” in order to provide the child population with effective medical care during the pandemic of the new coronaviral infection. The practical experience of specialists from various countries was considered during the development of this document. Special attention should be given to the evidence base of the presented data, as well as to the efficiency and safety issues of medications used in treatment of coronaviral infection and its complications. The authors highlight the problems of prevention, diagnostics and management of pathological conditions caused by COVID-19 in the article according to the presented guidelines. Patient’s management is presented depending on the age and severity of the disease itself. The therapy is considered with regard to etiological, pathogenetic and symptom focus