1,381 research outputs found
Plant regulation of microbial enzyme production in situ
Soil extracellular enzymes regulate the rate at which complex organic forms of nitrogen (N) become bio-available. Much research has focused on the limitations to heterotrophic enzyme production via lab incubations, but little has been done to understand the limitations to enzyme production in situ. We created root and symbiotic mycelia exclusion treatments using mesh in-growth bags in the field to isolate the effect of roots and other portions of the microbial community on enzyme production. When fertilized with complex protein N we found increases in N-degrading enzyme concentrations only when root in-growth was allowed. No response was observed when complex N was added to root-free treatments. Expanding on economic rules of microbial element limitation theory developed from lab incubation data, we suggest this is due to active transport of labile carbon (C) from roots to associated microbial communities in root bags. Roots alleviate C-limitation of microbial enzyme synthesis, representing a trade off between plants and microbes- plant C for microbial derived N
The ~11 yr Solar Cycle
Sunspot numbers and shifts in their distribution display a period of approximately 11 yr, a value sometimes uncritically applied to other measures of solar activity, direct and indirect, including the 10.7 cm radio flux, the inflow of galactic cosmic rays, solar flare frequency, terrestrial weather, and components of space climate, with a possible resulting loss of information. The ruling (Babcock) hypothesis and its derivatives link the sunspot cycle to dynamo processes mediated by differential solar rotation, but despite 60 years of observation and analysis the ~11 yr periodicity remains difficult to model; the possible contribution of planetary dynamics is also still controversial. The various solar sequences that genuinely display an ~11 yr cycle stand to benefit from an understanding of its periodicity that goes beyond statistical rigour. The outcome could ironically prompt the demotion of sunspots from their dominant historical role in favour of other possible indicators of solar cyclicity, such as the solar wind flux and its isotopic signatures, even if they are less accessible
Core to solar wind: a stepwise model for heating the solar corona
Operating experience from fusion research shows how Spitzer resistivity may render ohmic heating in the chromosphere self limiting and thus serve to define the lower margin of the transition region. Its upper margin is at about 6000 K, where radiative cooling of He:H plasma decelerates sharply. The third and last stage in the proposed scheme is expansion into the tenuous plasma of space, which leads to the acceleration of ions to high energies, long recorded by spacecraft instruments. There is thus dynamic continuity all the way from the solar interior, the energy source for spinning columns in the Rayleigh Benard setting of the convection zone, to the coronal exhalation of the solar wind, a finding which should benefit the analysis of space weather, witness the association between helium in the solar wind and the incidence of coronal mass ejections
Microbial carbon use efficiency predicted from genome-scale metabolic models
Respiration by soil bacteria and fungi is one of the largest fluxes of carbon (C) from the land surface. Although this flux is a direct product of microbial metabolism, controls over metabolism and their responses to global change are a major uncertainty in the global C cycle. Here, we explore an in silico approach to predict bacterial C-use efficiency (CUE) for over 200 species using genome-specific constraint-based metabolic modeling. We find that potential CUE averages 0.62āĀ±ā0.17 with a range of 0.22 to 0.98 across taxa and phylogenetic structuring at the subphylum levels. Potential CUE is negatively correlated with genome size, while taxa with larger genomes are able to access a wider variety of C substrates. Incorporating the range of CUE values reported here into a next-generation model of soil biogeochemistry suggests that these differences in physiology across microbial taxa can feed back on soil-C cycling.Published versio
The Uptake of Amino Acids by Microbes and Trees in Three Cold-Temperate Forests
Amino acids are emerging as a critical component of the terrestrial N cycle, yet there is little understanding of amino acid cycling in temperate forests. This research studied the uptake and turnover of amino acid N by soil microbes and the capacity of forest trees to take up the amino acid glycine in comparison to NH4+ and NO3ā. This research was conducted in three temperate forests located in northwest Connecticut, USA. The three forests differed in soil parent material and canopy tree species composition. At all three sites, amino acids were released from soil organic matter through the activity of proteolytic enzymes resulting in a pool of free amino acids in soil. Free amino acids were rapidly immobilized by soil microbes. A 15N-enriched-glycine-addition experiment also showed that a significant fraction of the amino acid N taken up by soil microbes was mineralized to NH4+ with substantial nitrification at one site. Tree species from all three sites had the physiological capacity to absorb the amino acid glycine but took up amino acid N, NH4+, and NO3ā in proportion to their availability in the soil. At the site with the highest gross fluxes of N, nearly all the N in amino acids was mineralized, and fine roots assimilated inorganic N much more rapidly than amino acid N. At the two sites with slower rates of gross amino acid production, the pool of free amino acids was larger, and fine roots assimilated amino acid N almost as fast as inorganic N. This study demonstrates that amino acids are an important component of the N cycle in temperate forests
Excluded-Volume Effects in Tethered-Particle Experiments: Bead Size Matters
The tethered-particle method is a single-molecule technique that has been
used to explore the dynamics of a variety of macromolecules of biological
interest. We give a theoretical analysis of the particle motions in such
experiments. Our analysis reveals that the proximity of the tethered bead to a
nearby surface (the microscope slide) gives rise to a volume-exclusion effect,
resulting in an entropic force on the molecule. This force stretches the
molecule, changing its statistical properties. In particular, the proximity of
bead and surface brings about intriguing scaling relations between key
observables (statistical moments of the bead) and parameters such as the bead
size and contour length of the molecule. We present both approximate analytic
solutions and numerical results for these effects in both flexible and
semiflexible tethers. Finally, our results give a precise,
experimentally-testable prediction for the probability distribution of the
distance between the polymer attachment point and the center of the mobile
bead.Comment: 4 pages, 3 figure
Elementary simulation of tethered Brownian motion
We describe a simple numerical simulation, suitable for an undergraduate
project (or graduate problem set), of the Brownian motion of a particle in a
Hooke-law potential well. Understanding this physical situation is a practical
necessity in many experimental contexts, for instance in single molecule
biophysics; and its simulation helps the student to appreciate the dynamical
character of thermal equilibrium. We show that the simulation succeeds in
capturing behavior seen in experimental data on tethered particle motion.Comment: Submitted to American Journal of Physic
Geometry, kinematics and rates of deformation in a normal fault segment boundary, central Greece
The geometry, kinematics and rates of deformation within a fault segment boundary between the ends of two major active normal fault segments have been investigated through examination of a faulted 126 ka marine terrace. Slipāvector azimuths defined by striations on the faults indicate NāS extension on c. EāW faults, subāparallel to those from earthquake focal mechanisms, together with significant and contemporaneous EāW extension on c. NāS faults. Summed rates of EāW extension along a c. 550 m transect (0.17 mm/yr) are comparable with those for NāS extension (0.20 mm/yr) along a c. 350 m transect. Our observations show that distributed nonāplane strain extension occurs in fault segment boundaries and this should be noted when studying faultātip fracture toughness and regional deformation rates
ET-1 plasma levels, choroidal thickness and multifocal electroretinogram in retinitis pigmentosa
Aim To assess the relationship between both photoreceptor function and choroidal thickness and endothelin-1 (ET-1) plasma levels in patients with early stage retinitis pigmentosa (RP). Main methods We compared 24 RP patients (14 males and 10 females), 25 to 42 years of age (mean age: 34 Ā± 7 years) with 24 healthy controls (12 males and 12 females) aged between 28 and 45 years (mean 36 Ā± 6.8 years). All patients underwent visual field test, electroretinogram and multifocal-electroretinogram and choroidal thickness measurement by using spectral domain optical coherence tomography. Key findings RP patients had a visual acuity of 0.95, a mean defect of the visual field of ā 7.90 Ā± 1.75 dB, a pattern standard deviation index of 6.09 Ā± 4.22 dB and a b-wave ERG amplitude of 45.08 Ā± 8.24 Ī¼V. Notably RP subjects showed significantly increased ET-1 plasma levels and reduced choroidal thickness compared with controls: respectively, 2.143 Ā± 0.258 pg/ml vs. 1.219 Ā± 0.236 pg/ml; p < 0.002 and 226.75 Ā± 76.37 Ī¼m vs. 303.9 Ā± 39.87 Ī¼m; p < 0.03. Spearman's correlation test highlighted that the increase of ET-1 plasma levels was related with the decrease of choroidal thickness (r = ā 0.702; p < 0.023) and the increase of implicit time in both ring 2 (r = ā 0.669; p < 0.034) and ring 3 (r = ā 0.883; p < 0.007) of mfERG. Significance Increased ET-1 plasma levels may play a key role in the impairment of retinal and choroidal blood flow due to the vasoconstriction induced by ET-1. This could lead to worsening of the abiotrophic process of the macular photoreceptors
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