Identifikasi Daging Ayam Broiler Dengan Pengamatan Struktur Histologis Identification of Broiler Meat With Histological Methods

A study to identification the microscopic structure of broiler meat by histological methods, has been carried out. The meat broiler samples were collected from musculus iliotibialis cranialis has been taken from Kupang supermarket. The tissue samples were fixed, dehydrated and embedded in paraffin and 5 μm sections. Harris-Haematoxilin-Eosin staining method, using to identified of histological structure. Microscopic analysis was performed using a binocular light microscope (100X). The study showed that, histological structure of broiler meat was composed by longitudinally and transverselly skeletal muscle. The nuclei in the periphery of the cell, there are dense connective tissue, fat, with small blood vessels. The skeletal myofibers diameter of muscle is 6,1 ± 0,8 μm. We observed for the presence of endomysium, perimysium and epimysium

Manajemen Kesehatan Ternak Babi Melalui Pemberdayaan Masyarakat Peternak Babi Skala Rumah Tangga

Kegiatan pengabdian ini bertujuan mengoptimalisasi pemberdayaan masyarakat peternak babi skala rumah tangga di desa Niukbaun, Kabupaten Kupang.  Kegiatan ini meliputi pelayanan kesehatan ternak babi, pemilihan bibit unggul, edukasi pengolahan pakan babi, dan edukasi manajemen perkandangan sehat. Ditinjau dari aspek kesehatan hewan, meningkatnya jumlah kematian ternak babi akibat beberapa penyakit pada babi, seperti diare, kecacingan dan saat ini, adalah penyakit ASF yang lebih membahayakan dibandingkan penyakit lain pada ternak babi dengan angka mortality rate yang tinggi. Kegiatan yang dilakukan meliputi 1) pertemuan dengan anggota kelompok peternak dari dua mitra; 2) pelayanan kesehatan ternak babi; 3) edukasi manajemen perkandangan babi sehat dan pemilihan bibit unggul, dan 4) edukasi pengolahan pakan babi dengan memanfaatkan bahan lokal. Secara umum kegiatan pengabdian kepada Masyarakat (PkM) berjalan dengan baik. Mitra kegiatan sangat antusias dalam berkoordinasi untuk pelayanan kesehatan ternak, mengolah pakan, dan mengikuti pemaparan tentang pemilihan bibit unggul dan pentingnya manajemen kesehatan ternak dan perkandagannya. Luaran yang dihasilkan antara lain publikasi kegiatan, peningkatan pemahaman mitra akan pentingnya kesehatan ternak babi, dan pemanfaatan bahan lokal untuk pakan ternak babi yang semuanya berlandaskan aspek kesejahteraan hewanKegiatan pengabdian ini bertujuan mengoptimalisasi pemberdayaan masyarakat peternak babi skala rumah tangga di desa Niukbaun, Kabupaten Kupang.  Kegiatan ini meliputi pelayanan kesehatan ternak babi, pemilihan bibit unggul, edukasi pengolahan pakan babi, dan edukasi manajemen perkandangan sehat. Ditinjau dari aspek kesehatan hewan, meningkatnya jumlah kematian ternak babi akibat beberapa penyakit pada babi, seperti diare, kecacingan dan saat ini, adalah penyakit ASF yang lebih membahayakan dibandingkan penyakit lain pada ternak babi dengan angka mortality rate yang tinggi. Kegiatan yang dilakukan meliputi 1) pertemuan dengan anggota kelompok peternak dari dua mitra; 2) pelayanan kesehatan ternak babi; 3) edukasi manajemen perkandangan babi sehat dan pemilihan bibit unggul, dan 4) edukasi pengolahan pakan babi dengan memanfaatkan bahan lokal. Secara umum kegiatan pengabdian kepada Masyarakat (PkM) berjalan dengan baik. Mitra kegiatan sangat antusias dalam berkoordinasi untuk pelayanan kesehatan ternak, mengolah pakan, dan mengikuti pemaparan tentang pemilihan bibit unggul dan pentingnya manajemen kesehatan ternak dan perkandagannya. Luaran yang dihasilkan antara lain publikasi kegiatan, peningkatan pemahaman mitra akan pentingnya kesehatan ternak babi, dan pemanfaatan bahan lokal untuk pakan ternak babi yang semuanya berlandaskan aspek kesejahteraan hewa

KAJIAN HISTOKIMIA SEBARAN KARBOHIDRAT ASAM PADA LAMBUNG DEPAN SAPI SUMBA ONGOLE (Bos indicus)

Sumba ongole (Bos indicus) is one of the Indonesian local cattle breeds that has a high number of carcasses and good adaptability to the dry climate and low humidity on the island of Sumba. Cattle have a forestomach consisting of the rumen, reticulum, and omasum, which functions to ferment and absorb nutrition. This study aims to determine the distribution of acid carbohydrates in the rumen, reticulum, and omasum of sumba ongole cattle. Six samples of the rumen, reticulum, and omasum were collected from East Sumba Slaughter House, fixed in formalin 10 %, processed histologically, and continued with alcian blue (AB) staining. The result showed the various/different intensity of acid carbohydrates in each of the tunica of the rumen, reticulum, and omasum. The distribution is mostly found in the stratum corneum lamina epithelium. The results of this study indicate that the stratum corneum is the layer that is more frequently exposed to food that requires acid carbohydrates in its function to protect the forestomach as well as to lubricate the food to make it easier to digest

Studi Anatomi Catecholamine Mesolimbic Pathway pada Kalong Kapauk (Pteropus vampyrus) Asal Pulau Timor

Catecholamine mesolimbic pathway (CMP) is a dopamine pathways of the brain that derived from cell bodies in the ventral tegmental area (VTA) to the limbic area i.e. nucleus accumbens, amygdala and hippocampus. In normal conditions, CMP plays a role as controls of motor activity, motivation, emotional and cognitive. Bats are flying mammals which can be potential as natural reservoir of rabies. One of the symptoms of animal rabies is impaired in emotional control that related with limbic system. Kalong kapauk (Pteropus vampyrus) is one of the species of bats that distributed in south Sumatra, Java, Borneo, and spread out to the island of Timor, East Nusa Tenggara. The aim of this research is studying the anatomical structures in CMP of kalong kapauk (Pteropus vampyrus) from Timor island. Four kalong kapauk (Pteropus vampyrus) from Soe-Timor island, East Nusa Tenggara was anaesthetized by using ketamine (20 mg/kg bw) and xylazin (2 mg/kg bw). In deep anesthesia condition, animals were perfused by using physiological saline and after the blood were removed well, the physiological saline were changed to 10% buffered formalin as a fixative. The brain were removed from the cranium, dissected midsagital and processed for histology by paraffin method. The brains were cut in 12 μm thickness and then it stained by using cresyl echt violet and immunohistochemistry by tyrosine hydroxilase antibody. The sections were examined for shape and size of neurons in the VTA and their axonal pathways by light microscope and were documented using a digital camera. The results were analyzed descriptively. The results shows that neurons in the VTA are bipolar and multipolar in shape with the size of 10-32 μm (mean 20.31 ± 4.40 μm) and densities are 15.33 ± 5.71 cells/0,116 mm2. The area of nucleus accumbens, amygdala, and cornua ammonis 3 of hippocampal are TH immunoreactive as the axon terminal. The conclusion of this study is there are catecholaminergic neurons in the VTA that made an area limbic pathways i.e. nucleus accumbens, amygdala, and cornua ammonis 3 of hippocampal

Parameter fisiologi kambing kacang (Capra aegagrus hircus) di Desa Nunkurus Kecamatan Kupang Timur Kabupaten Kupang

 Goat are herbivoreus ruminant animals that commonly farm in Indonesia. Goats have advantages like quick to breed and can adapt in different environments. Goats played importantely in social and culture in Nunkurus Kupang district, goat can have an important role in the field of animal husbandry in the region. The purpose of this study was to determine the physiology parameter of goat in Nunkurus. The measured parameter are body temperature, respiration and pulse. This research uses as much as 36  kacang goats. Sample are grouped into 3 groups based on age. Child group with age 2 to 8 months, young 8 to 12 months and adult >12 months. In each group consist of 6 males and 6 females. The data about fisiology value taken in morning and afternoon in one week for each aged group. The results showed that average of kacang goat body temperature in Nunkurus at the morning is 38,8 ± 0,09°C to 39,7 ± 0,05°C and at the afternoon is 38,9 ± 0,2°C to 40,1 ± 0,1°C. The respiration average at the morning is 14,28 ± 1,1 to 19,42 ± 1,1 times/minute and at the afternoon is 16,85 ± 0,48 to 21,14 ± 0,78  times/minute. The pulse at the morning is 74 ± 1,39 to 99 ± 0,9 times/minute and at the afternoon is 76 ± 1,13 to 102 ± 0,75 times/minute. It is concluded that the value of fisiology parameter in Nunkurus Village is in the same range with the other area in Indonesia

Studi Anatomi Lambung Kelelawar Buah (Pteropus vampyrus) Dengan Pewarnaan Histokimia Periodic Acid Shiff (PAS)

Timor Island, East Nusa Tenggara (NTT) has a large enough population of bats. A fruit bat (Pteropus vampyrus) is one of such species. Gastric in mammals consist of three region that is fundus, cardia and pylorus. This study aims to determine whether there are differences in the distribution pattern of constituent cells of gastric in Pteropus vampyrus to other mammals. Pteropus vampyrus is anaesthetized with ketamine 20 mg/kg and xylazine 2 mg/kg intramuscularly. In the anesthetized state, the perfusion of the heart is done by opening the chest cavity. Further observation is to the gastric in macroanatomy and then gastric organs were fixed by 10% formalin and then stain with histochemical staining (PAS). Region of Fundus of the stomach area occupies most of the region compared to the cardia and pylorus. Cardia and pyloric region are dominated by the parietal cells and mucous neck cells on the surface, but the pyloric region has begun to form gastric pits. Fundus region is dominated by the constituent cells such as gastric chief cells and parietal cells. Based on the results of the study, it is concluded that the distribution pattern in Pteropus vampyrus chief cells, parietal and mucous neck cells are different from other mammals

The Anatomy of Asian Palm Civet (Paradoxurus hermaphroditus) Brain in Timor Island

Asian palm civet (Paradoxurus hermaphroditus) is native to some Asian regions, include Indonesia. Despite its wide spreading in those areas, study in regards to its anatomy is very few. This study is aimed to unveil the anatomy of Asian plam civet brain in Timor island. This study was performed in three Asian palm civets. The palm civets were anesthesized and humanely euthanazied using perfusion technique, and macroscopically observed for their brains anatomy.  Results showed Asian palm civet consisted of three main parts which were cerebrum, cerebellum, and brain stem. Compared to brain of dog, sulci and gyri of the Asian palm civet is simpler, with paraflocculus lobes were merely located in dorsal and ventral parts of the brain. However, the vermis was more similar to dogs, and more developed compare to Reusettussp (Javanese codot). This suggests that the anatomical difference is a result of the different behavior, which are nocturnal animal and live on trees

DISTRIBUSI KARBOHIDRAT NETRAL PADA LAMBUNG DEPAN SAPI SUMBA ONGOLE (Bos indicus)

Sumba Ongole cattle (Bos indicus) is a superior livestock commodity in East Nusa Tenggara Province, especially on the island of Sumba. Cattle have a forestomach consisting of the rumen, reticulum, and omasum. This forestomach plays an important role in carbohydrate fermentation. This study aims to determine the distribution of neutral carbohydrates in the forestomach of Sumba Ongole cattle. Six samples of the rumen, reticulum, and omasum were collected from East Sumba Slaughter House. The tissue was fixed in formalin 10 %, continued with processed histologically and PAS staining. The result showed that neutral carbohydrates distributed in the tunica mucosa and tunica muscularis rumen, reticulum, and omasum with a weak (+) to strong (+++) reaction intensity staining. The factors that influence the difference in color intensity in each tunica of the rumen, reticulum, and omasum are related to the function and mucus secretion of each cell

GAMBARAN PATOLOGI ANATOMI PADA BABI LANDRACE SUSPECT AFRICAN SWINE FEVER (ASF) DI KABUPATEN KUPANG

African Swine Fever (ASF) is a viral disease that attacks pigs and to date has caused many pig deaths in Kupang Regency. ASF is caused by a double-stranded DNA virus from the Asfivirus genus and the Asfarviridae family. This research aims to determine the anatomical pathology of the swine landrace suspect ASF. Organ samples were collected from two male landrace pigs and two female landrace pigs, aged 7 months, from Oeltuah Village, Taebenu District and Tarus Village, Central Kupang District, Kupang Regency, NTT. Clinical examinations were carried out on sick animals that were found during the investigation, then necropsied on the dead animals were carried out and continued with anatomical pathology examinations at the Pathology Laboratory, Faculty of Veterinary Medicine, Nusa Cendana University. Anatomical pathology examinations are carried out by observing changes in the structure and appearance of the organs. The necropsy results showed sub-cutaneous ecchymosis hemorrhage in the abdomen, limbs and ears, gastric, intestinal and hepatic hemorrhage, hemorrhagic lymphadenitis in mesenteric lymph nodes, hyperemic splenomegaly, pteckie hemorrhage in the renal capsule,, multifocal hemorrhage in the renal medulla and pulmonary lobe. Based on the observation of clinical symptoms and changes in anatomical pathology, it can be concluded that the death of pigs was suspected to be caused by the suspect ASF

Carbohidrate Distribution In The Small Intestine of Sumba Ongole Cattle (Bos indicus)

The small intestine has cells that function to secrete mucus that protects the intestine from pathogenic agents and mechanical damage. One of the components of mucus is carbohydrates. This study aims to knowing the distribution of acidic and neutral carbohydrates in the small intestine of sumba ongole (Bos indicus) cattle. Six samples of the small intestine were collected from East Sumba Slaughter House. The tissue was fixed in formalin 10 %, continued with processed histologically and AB-PAS staining. The results showed that acidic and neutral carbohydrates were distributed in the tunica of the duodenum, jejunum, and ileum with varying intensity. The strong intensity was seen in goblet cells, Lieberkuhn crypts, and Brunner's glands. The different distribution of carbohydrates in the small intestine is related to the mucus secretion of each cell and that function