Il Concordato Preventivo Con Riserva

Trattazione del concordato preventivo con riserva: dalla sua nascita agli elementi problematici, il tutto passando per un'analisi dettagliata dell'istituto

A Framework to Evaluate Information Quality in Public Administration Website

This paper presents a framework aimed at assessing the capacity of Public Administration bodies (PA) to offer a good quality of information and service on their web portals. Our framework is based on the extraction of “.it” domain names registered by Italian public institutions and the subsequent analysis of their relative websites. The analysis foresees an automatic gathering of the web pages of PA portals by means of web crawling and an assessment of the quality of their online information services. This assessment is carried out by verifying their compliance with current legislation on the basis of the criteria established in government guidelines[1]. This approach provides an ongoing monitoring process of the PA websites that can contribute to the improvement of their overall quality. Moreover, our approach can also hopefully be of benefit to local governments in other countries. Available at: https://aisel.aisnet.org/pajais/vol5/iss3/3

Access control: AAAA where the first two As stand for Ability Authentication

We investigate the notion of authentication procedure. We show how this can be split in several phases: establishing that an ability can be performed, deducing a certain information from such an ability, witnessing the authenticity of the information deduced by the ability. In our framework, ability means that that one is able to perform role in a protocol. We argue that the concept of ability authentication is more general that the one of identity authentication, that is commonly used in AAA systems. We then develop an architecture for managing abilities and we show some application scenarios

Receptor-based virtual screening evaluation for the identification of estrogen receptor β ligands.

In this paper, a receptor-based virtual screening study for the identification of estrogen receptor β (ERβ) ligands was developed. Starting from a commercial database of 400,000 molecules, only six compounds resulted to be potential active ligands of ERβ. Interestingly, all the six molecules possess scaffolds that had already been reported in known ERβ ligands. Therefore, the results obtained herein confirm the reliability of our virtual screening procedure, thus encouraging the application of this protocol to larger commercial databases in order to identify new ERβ ligands

Proposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt Virus

Viruses provoke considerable economical losses in agriculture. New molecular approaches to develop genetic resistance based on translational genomics and precision genetic modifications are highly expected. The type II Clustered, Regularly Interspaced Palindromic Repeats (CRISPR) system including Cas9 nuclease represent a promising and very powerful tool to specifically modulate the expression and activity of genes involved in biotic stress responses. In this study, we describe an approach to develop a platform system based on CRISPR system for genome editing technology in tomato. Tomato is an excellent plant for this approach considering the high-quality genome sequence, the rapid life cycle, the highly efficient in vitro plant culture and transformation protocols, Genome editing can be used to allow resistance to Tomato Spotted Wilt Virus (TSWV) infections by the successful obtainment of two specific objectives: (1) Development of a Genome Editing (GE) system using CRISPR-Cas9 system in tomato (Objective 1) and (2) test the system in inducing genetic resistance to TSWV infections. First, it will be necessary to model the molecular dynamics of key host and pathogen proteins predicting how targeted mutations affect these interactions. Then these host players will be targeted by CRISPR-Cas9 technology. The obtained plants can be evaluated for their phenotypic resistance and deeply analyzed using "omic" platforms to gain insight into gene regulatory networks of plant resistance. Outcomes of the proposed project will be essentially three: (1) Identify host target proteins interacting with pathogenic proteins and model their dynamic interactions; (2) develop a platform technology usable to obtain resistant tomatoes to TSWV inducing targeted genetic modifications in the genome; (3) facilitate the adaption of this platform to the improvement of important traits in other specialty crops

Identification of Lactate Dehydrogenase 5 Inhibitors using Pharmacophore- Driven Consensus Docking

Background: Human lactate dehydrogenase 5 (hLDH5) represents a promising anticancer target, particularly for the treatment of hypoxic tumors, where it is often hyperexpressed. In fact, by catalyzing the reduction of pyruvate to lactate, hLDH5 allows the survival of tumor cells under hypoxic conditions by means of glycolysis. Despite the efforts dedicated to the identification and development of hLDH5 inhibitors, only few compounds showing promising activity in cancer cell lines have been reported. Objective: In the present study, we developed a virtual screening (VS) protocol aimed at identifying new small molecule inhibitors of hLDH5. Method: The VS strategy consisted in a pharmacophore-driven consensus docking (CD) approach, combining a structure-based pharmacophore screening and CD protocol employing three different docking methods. Results: The VS protocol was applied to filter the Enamine commercial database and allowed the selection of three candidate ligands to be subjected to hLDH5 inhibition assays. One of the selected compounds showed a promising activity, compared to its low molecular weight, with an IC50 of 180.7 ± 16.5 μM. Conclusion: We identified a new small-molecule inhibitor of hLDH5 that can be considered as a new lead for the development of potent hLDH5 inhibitors. Moreover, these results demonstrate the reliability of the VS protocol developed

Application of microwave irradiation for the removal of polychlorinated biphenyls from siloxane transformer and hydrocarbon engine oils

The removal of polychlorinated biphenyls (PCBs) both from siloxane transformer oil and hydrocarbon engine oil was investigated through the application of microwave (MW) irradiation and a reaction system based on polyethyleneglycol (PEG) and potassium hydroxide. The influence of the main reaction parameters (MW irradiation time, molecular weight of PEG, amount of added reactants and temperature) on the dechlorination behavior was studied. Promising performances were reached, allowing about 50% of dechlorination under the best experimental conditions, together time and energy saving compared to conventional heating systems. Moreover, an interesting dechlorination degree (up to 32%) was achieved for siloxane transformer oil when MW irradiation was employed as the unique driving force. To the best of our knowledge, this is the first time in which MW irradiation is tested as the single driving force for the dechlorination of these two types of PCB-contaminated oils

Salicylaldoxime derivatives as new leads for the development of carbonic anhydrase inhibitors

n/

Development of terphenyl-2-methyloxazol-5(4H)-one derivatives as selective reversible MAGL inhibitors

Monoacylglycerol lipase is a serine hydrolase that plays a major role in the degradation of the endocannabinoid neurotransmitter 2-arachidonoylglycerol. A wide number of MAGL inhibitors are reported in literature; however, many of them are characterised by an irreversible mechanism of action and this behavior determines an unwanted chronic MAGL inactivation, which acquires a functional antagonism of the endocannabinoid system. The possible use of reversible MAGL inhibitors has only recently been explored, due to the lack of known compounds possessing efficient reversible inhibitory activities. In this work, we report a new series of terphenyl-2-methyloxazol-5(4H)-one derivatives characterised by a reversible MAGL-inhibition mechanism. Among them, compound 20b showed to be a potent MAGL reversible inhibitor (IC50= 348 nM) with a good MAGL/FAAH selectivity. Furthermore, this compound showed antiproliferative activities against two different cancer cell lines that overexpress MAGL