Additional file 1: of Exon-intron structure and sequence variation of the calreticulin gene among Rhipicephalus sanguineus group ticks

Figure S1. Alignment of the full sequence of the crt region amplified in the Rhipicephalus spp. individuals analysed (coded as in Table 1). Nucleotides in orange boxes belong to exon regions in 5′ and 3′ splice donor regions. Nucleotides in light grey boxes belong to intron region in 5′ and 3′ splice donor regions. Nucleotides in black boxes indicate sequence identity in intron region. Asterisks show nucleotide identity in exon regions. Numbers indicate the nucleotide position of the R. sanguineus (s.l.) crt sequence AY395275 [25]. Abbreviations: R.s. Trop. lin., Rhipicephalus sanguineus (s.l.) “Tropical lineage”; R.s. Temp. lin., Rhipicephalus sanguineus (s.l.) “Temperate lineage”. (TIF 1069 kb

Additional file 2: of Biological compatibility between two temperate lineages of brown dog ticks, Rhipicephalus sanguineus (sensu lato)

Partial cytochrome c oxidase subunit 1 (cox1) gene sequences generated in this study. (FAS 60 kb

Number of <i>O. lupi</i> microfilariae retrieved by soaking skin samples from each anatomical site collected at the necropsy of dog 1.

<p>Larvae were counted after 6 h and 12 h of soaking (i.e. replicate 1 and 2) by reading two aliquots (each by 20 µl) of sediment.</p

Additional file 1: Figure S1. of Paramyosin of canine Onchocerca lupi: usefulness for the diagnosis of a neglected zoonotic disease

Epitopes predicted by bioinformatics and those reported in the literature for other organisms mapped to Onchocerca lupi paramyosin. Residues underlined indicate T-cell epitopes whilst those in bold indicate B-cell epitopes. Of the 874 predicted antibody epitopes, when compared with those predicted for Acanthocheilonema viteae, Brugia malayi, Dirofilaria immitis, Loa loa, Onchocerca volvulus and Wuchereria bancrofti, 621 were unique to Onchocerca lupi. (DOCX 32 kb

Microfilaria of <i>Onchocerca lupi</i>.

<p>Microfilaria of <i>Onchocerca lupi</i> found in the skin sediment of a dog (Scale bar: 50 µm).</p

Distribution of microfilariae of <i>Onchocerca lupi</i>.

<p>Overall (A) and individual (B) mean number of microfilariae of <i>Onchocerca lupi</i> found in each body site of dogs 2–6.</p

Circadian rhythm of <i>Onchocerca lupi</i> microfilariae.

<p>Overall (A) and individual (B) mean number of microfilariae of <i>Onchocerca lupi</i> retrieved from dogs 2–6, in the morning, late afternoon and at night.</p

<i>Onchocerca lupi</i> microfilariae in the skin sediment.

<p>Several <i>Onchocerca lupi</i> microfilariae collected from the right ear of dog 1 (second replicate) (Scale bar: 100 µm).</p

Tick-borne pathogens identified during the study.

<p>(A) First-stage larvae (L1) of <i>Cercopithifilaria bainae</i> retrieved in a skin snip of a dog (scale-bar = 50 µm); (B) <i>Hepatozoon canis</i> gamont (scale-bar = 20 µm); (C) <i>Anaplasma platys</i> (scale-bar = 20 µm).; (D) <i>Babesia vogeli</i> trophozoites retrieved at blood-smear examination (scale-bar = 20 µm).</p

Incidence density rate (IDR) of positive animal-month at risk for <i>Cercopithifilaria bainae</i>-infected dogs throughout the study period.

*<p>These numbers represent the negative dogs that have been included in the cohort for calculating the IDR at each sampling time.</p