Delayed Insemination of Non-Estrual Beef Heifers in 7-day CO-Synch Timed Artificial Insemination

Reproductive performance in cattle is considered to be the most economically important trait and is essential for the success of an operation. In the last decade, timed artificial insemination (AI) in the beef cattle industry has increased in popularity due to the decreased amount of labor required for estrous detection. To increase the use of AI; however, cost and time inputs need to be further decreased. The objective of this study was to determine if fertility could be improved in beef heifers that are not expressing estrus before timed AI by delaying insemination

Intensive Rotational Grazing Systems for Dairying in a Subtropical Environment: Animal, Plant, and Soil Responses

Forage species, stocking rate, and supplementation rate effects upon performance of lactating dairy cows were studied. Forage mass and nutritive value and nutrient concentration in shallow groundwater also were measured. Cows (n = 44) grazed bermudagrass (Cynodon dactylon) or rhizoma peanut (Arachis glabrata), stocked at 4.9 or 7.4 cows ha-1, and 2.5 or 4.9 cows ha-1, respectively. Pelleted concentrate:whole cottonseed (80:20) was offered twice daily post milking at 1 kg per 2 or 3 kg of daily milk production. Cows grazing rhizoma peanut produced more (P = .076) milk per day but had greater (P = .028) loss of body condition. Higher stocking rates caused greater (P \u3e .070) weight loss. Greater supplementation increased (P \u3c .05) production of milk, fat and protein, but also increased (P \u3c .044) weight and body condition losses. Higher stocking rates and lower supplementation reduced pasture (P \u3c .001) herbage mass. Rhizoma peanut herbage was higher in nutritive value than bermudagrass. Nutrient concentrations in shallow groundwater were not affected by treatments. Use of pasture for lactating cows has potential in Florida, but low milk production and loss of body condition during summer pose management challenges

Carbon Isotope and Lipid Biomarker Stratigraphy from Organic-Rich Strata Through the Neoproterozoic Shuram Excursion in South Oman

The regulation of oxygen levels in Earth’s atmosphere and oceans is inextricably linked to the carbon cycle. Carbon isotope ratios of carbonate and sedimentary organic matter provide first order insights into the operation of the carbon cycle in the geologic past. During the Ediacaran period, the ~580 Ma ‘Shuram Excursion’ (SE) records a dramatic, systematic shift in δ^(13)C_(carbonate) values to as low as cɑ. -12‰, lasting potentially millions to tens of millions of years in duration and constitutes the largest carbon isotope excursion known in the record [1]. The extremely negative carbon isotope values in carbonate challenges our understanding of the ancient carbon cycle and is difficult to rationalise via uniform carbon cycle principles. Several hypotheses have been developed to explain this behaviour, all of which make different predictions for the abundance, structure, and isotopic composition of organic carbon through the excursion. For a direct test of these ideas, we report paired organic and inorganic stable carbon isotope ratios in addition to detailed lipid biomarker stratigraphic records from a subsurface well drilled on the eastern flank of the South Oman Salt Basin, Sultanate of Oman. This well captures thermally immature and organic-rich Nafun Group strata traversing the SE, yielding variable but primary biomarker characteristics typical of Neoproterozoic rocks from this region. Despite the high organic matter contents, the carbon isotopic compositions of carbonates do not covary with those of organic phases. Furthermore, lipid biomarker data reveal that organic matter composition and source inputs varied stratigraphically, reflecting biological community shifts in non-migrated, syngenetic organic matter deposited during this interval. Together these observations imply that carbonateorganic isotopic decoupling during the SE is not a result of mixing of fossil or exogenous carbon sources (either DOC, detrital, or migrated) with syngenetic organic matter

Results of a production analysis survey of cow herds in Kansas

A survey of production levels and management practices of 205 cow herds representing over 26,000 cows in Kansas was conducted in 1991. These operations were located throughout Kansas except for the Northwest corner. Emphasis was placed on determining levels of production and reproductive parameters. Breeders emphasized calf crop. For example, the average calf crop was 91.6%, with 4.3% open females and 4.4% calf death loss. Cumulative calving percentages by 21-day calving periods were 32, 55, and 68%. Average weaning weights were 550 lb. for steers and 515 lb for heifers. Additionally, information was collected on breeding, nutrition, health, and general management practices

Switchgrass Response to Nitrogen Fertilizer Across Diverse Environments in the USA: a Regional Feedstock Partnership Report

The Regional Feedstock Partnership is a collaborative effort between the Sun Grant Initiative (through Land Grant Universities), the US Department of Energy, and the US Department of Agriculture. One segment of this partnership is the field-scale evaluation of switchgrass (Panicum virgatum L.) in diverse sites across the USA. Switchgrass was planted (11.2 kg PLS ha−1 ) in replicated plots in New York, Oklahoma, South Dakota, and Virginia in 2008 and in Iowa in 2009. Adapted switchgrass cultivars were selected for each location and baseline soil samples collected before planting. Nitrogen fertilizer (0, 56, and 112 kg N ha−1 ) was applied each spring beginning the year after planting, and switchgrass was harvested once annually after senescence. Establishment, management, and harvest operations were completed using fieldscale equipment. Switchgrass production ranged from 2 to 11.5 Mg ha−1 across locations and years. Yields were lowest the first year after establishment. Switchgrass responded positively to N in 6 of 19 location/year combinations and there was one location/year combination (NY in Year 2) where a significant negative response was noted. Initial soil N levels were lowest in SD and VA (significant N response) and highest at the other three locations (no N response). Although N rate affected some measures of biomass quality (N and hemicellulose), location and year had greater overall effects on all quality parameters evaluated. These results demonstrate the importance of local field-scale research and of proper N management in order to reduce unnecessary expense and potential environmental impacts of switchgrass grown for bioenergy

The Polyamine Inhibitor Alpha-Difluoromethylornithine Modulates Hippocampus-Dependent Function after Single and Combined Injuries

Exposure to uncontrolled irradiation in a radiologic terrorism scenario, a natural disaster or a nuclear battlefield, will likely be concomitantly superimposed on other types of injury, such as trauma. In the central nervous system, radiation combined injury (RCI) involving irradiation and traumatic brain injury may have a multifaceted character. This may entail cellular and molecular changes that are associated with cognitive performance, including changes in neurogenesis and the expression of the plasticity-related immediate early gene Arc. Because traumatic stimuli initiate a characteristic early increase in polyamine metabolism, we hypothesized that treatment with the polyamine inhibitor alpha-difluoromethylornithine (DFMO) would reduce the adverse effects of single or combined injury on hippocampus structure and function. Hippocampal dependent cognitive impairments were quantified with the Morris water maze and showed that DFMO effectively reversed cognitive impairments after all injuries, particularly traumatic brain injury. Similar results were seen with respect to the expression of Arc protein, but not neurogenesis. Given that polyamines have been found to modulate inflammatory responses in the brain we also assessed the numbers of total and newly born activated microglia, and found reduced numbers of newly born cells. While the mechanisms responsible for the improvement in cognition after DFMO treatment are not yet clear, the present study provides new and compelling data regarding the potential use of DFMO as a potential countermeasure against the adverse effects of single or combined injury

Building Partnerships to Address Social and Technological Challenges to Enhance Farm Profitability and Improve Water Quality Through Better Grassland Management

With 2.1 million acres of pastureland and 1.25 million acres of hay land in Virginia, the rural Virginia landscape is predominately grassland. These lands form the base of the $3.96 billion-dollar livestock and dairy industry in Virginia. Managing these livestock in a profitable manner for farmers and beneficial to the environment is important. A cultural tradition with roots in colonial times has been to run animals in large fields year-round throughout Virginia. Livestock often graze from spring until fall (about 220 days), and farmers feed hay the remainder of the year. Spikes in the cost of fuel, fertilizer, and equipment are making traditional grazing/haying systems less profitable. The Virginia Cooperative Extension Farm Enterprise budgets show that that the cost of hay accounts for over 50$75 per cow by extending their grazing season. The same phenomenon applies to other types of grazing livestock. If ten percent of the livestock producers in the state adopted better grazing management to extend their grazing season by 60 days, profitability is expected for Virginia grazing livestock producers by over $5 million per year. Practices such as rotational grazing and stream exclusion are directly tied to National and State goals to improve water quality in the Chesapeake Bay. Virginia’s Phase III WIP (Chesapeake Bay Watershed Improvement Plan) seeks the exclusion of livestock from all perennial streams and achieving good rotational grazing practices on 347,000 acres of pasture. A number of agencies and private sector groups have been providing cost share and technical guidance to incentivize livestock stream exclusion and the installation of pasture management infrastructure. Installation is only part of the challenge. Farmers also need to be taught how to how to manage the system in a profitable manner and have been slow to adopt good pasture management practices. Preliminary data show that 87% of Virginia’s cow-calf producers manage their grasslands using traditional methods. Only six percent have extended their grazing season beyond 265 days

Endothelial cells regulate p53-dependent apoptosis of neural progenitors after irradiation

Endothelial cells represent an important component of the neurogenic niche and may regulate self-renewal and differentiation of neural progenitor cells (NPCs). Whether they have a role in determining the apoptotic fate of NPCs after stress or injury is unclear. NPCs are known to undergo p53-dependent apoptosis after ionizing radiation, whereas endothelial cell apoptosis after irradiation is dependent on membrane acid sphingomyelinase (ASMase) and is abrogated in sphingomyelin phosphodiesterase 1 (smpd1)- (gene that encodes ASMase) deficient mice. Here we found that p53-dependent apoptosis of NPCs in vivo after irradiation was inhibited in smpd1-deficient mice. NPCs cultured from mice, wild type (+/+) or knockout (−/−), of the smpd1 gene, however, demonstrated no difference in apoptosis radiosensitivity. NPCs transplanted into the hippocampus of smpd1−/− mice were protected against apoptosis after irradiation compared with those transplanted into smpd1+/+ mice. Intravenous administration of basic fibroblast growth factor, which does not cross the blood–brain barrier, known to protect endothelial cells against apoptosis after irradiation also attenuated the apoptotic response of NPCs. These findings provide evidence that endothelial cells may regulate p53-dependent apoptosis of NPCs after genotoxic stress and add support to an important role of endothelial cells in regulating apoptosis of NPCs after injury or in disease