Management Of Mandibular Lateral Incisor Agenesis With Skeletal Class III Malocclusion by Space Closing Technique

Background: Patients with congenital absence of a mandibular lateral incisor are often found having difficulty in achieving adequate functional occlusion. It may affect esthetics, mastication, speech, and occlusal balance. Purpose: This paper reported an agenesis treatment of one mandibular lateral incisor case using a space closure method. Case: A twenty-three years old female patient with agenesis of tooth 42, mandibular anterior crowding, multiple diastema on mandibular anterior teeth, and skeletal class III malocclusion. Case Management: Space closure method was chosen to correct the agenesis by considering the class III skeletal malocclusion and multiple diastema condition. Conclusion: Space closure method treatment improved the patient’s facial and dental esthetics, and it provided a good functional occlusion, despite the absence of a mandibular lateral incisor, which generally impairs the adequate incisal guidanc

Post Oral Administration of Epigallocatechin Gallate from Camelia sinensis Extract Enhances Vascular Endothelial Growth Factor and Fibroblast Growth Factor Expression during Orthodontic Tooth Movement in Wistar Rats

Background:East Java green tea leaf (Camelia sinensis) possesed active compound such as Epigallocatechin Gallate (EGCG) is well known for enhancing the bone remodelling through enhancement of Vascular Endothelial Growth Factor (VEGF) and Fibroblast Growth Factors (FGF-2). Remodelling of alveolar bone is very important to obtain optimal Orthodontic Tooth Movement (OTM) to align the tooth. Aim: To investigate the expression of VEGF and FGF-2 expression during OTM in Wistar rat after administration of EGCG from C. sinensis Extract (EGCG-CSE) Wistar rats. Material and Methods: This study was true experimental study with post-test only control group design. Twenty eight Wistar rats were randomly selected and divided into four groups accordingly; K- group which did not get both EGCGCSE administration and OTM; K+ group with OTM for 14 days, but no EGCG-CSE administration; 1 (T1) with 4 days of OTM and 7 days of EGCG-CSE administration; treatment group 2 (T2) with both 14 days OTM and EGCG-CSE administration. Ten g force/mm2 of NiTi close coil spring was installed between the upper left molars and cental insicive to move the molar mesially that induce OTM. All OTM animal model were terminated in the 14th days. Maxillary was isolated for immunohistochemistry investigation. Tukey Honest Significant Difference(HSD) was done after Analysis of Variance (ANOVA) test to investigate the significant difference betweengroups (p<0.05). Results: The highest positive VEGF expression was found in the T2 in both area. Meanwhile, the highest positive FGF-2 expression was found in the K-group in both area. There were significant different of VEGF and FGF-2 expression in both area between groups except T1 and T2. Conclusion: Post administration of EGCG-CSE can stimulate the VEGF and FGF-2 expression during OTM in Wistar rats

High Mobility Group Box 1 and Heat Shock Protein-70 Expression Post (-)-Epigallocatechin-3-Gallate in East Java Green Tea Methanolic Extract Administration During Orthodontic Tooth Movement in Wistar Rats

Objective: To investigate the expression of High Mobility Group Box 1 (HMGB1) and Heat Shock Protein-70 (HSP-70) during orthodontic tooth movement (OTM) after (-)- Epigallocatechin-3-Gallate(EGCG) in East Java Green Tea (Camelia Sinensis) Methanolic Extract (GTME) administration in vivo. Material and Methods: 28 Wistar rats (Rattus Novergicus) was used and divided into 4 groups accordingly: K- without EGCG and OTM; K+ with OTM, without EGCG for 14 days; T1with OTM for 14 days and EGCG for 7 days; treatment group 2 (T2) with OTM and EGCG for 14 days. OTM animal model was achieved through the installation of the OTM device by means of NiTi close coil spring with 10g force placed between the first incisor and first maxillary molars. The samples were terminated on Day 14. The pre-maxillary was isolated for the immunohistochemical examination. Analysis of Variance (ANOVA) then continued with Tukey Honest Significant Difference (HSD) (p<0.05) was performed to analyze the data. Results: The highest HMGB1 and HSP-70 expression were found in the K+ group pressure side, meanwhile the lowest HMGB1 and HSP-70 expression were found in K- group tension side in the alveolar bone. There was a significant decrease of HMGB1 and HSP-70 expression in T2 compared to T1 and K+ with significant between groups (p<0.05; p=0.0001). Conclusion: The decreased expression of HMGB1 and HSP-70 in alveolar bone of OTM wistar rats due to post administration of GTME that consisted EGCG

High Mobility Group Box 1 and Heat Shock Protein-70 Expression Post (-)-Epigallocatechin-3-Gallate in East Java Green Tea Methanolic Extract Administration During Orthodontic Tooth Movement in Wistar Rats

Objective: To investigate the expression of High Mobility Group Box 1 (HMGB1) and Heat Shock Protein-70 (HSP-70) during orthodontic tooth movement (OTM) after (-)- Epigallocatechin-3-Gallate (EGCG) in East Java Green Tea (Camelia Sinensis) Methanolic Extract (GTME) administration in vivo. Material and Methods: 28 Wistar rats (Rattus Novergicus) was used and divided into 4 groups accordingly: K- without EGCG and OTM; K+ with OTM, without EGCG for 14 days; T1with OTM for 14 days and EGCG for 7 days; treatment group 2 (T2) with OTM and EGCG for 14 days. OTM animal model was achieved through the installation of the OTM device by means of NiTi close coil spring with 10g force placed between the first incisor and first maxillary molars. The samples were terminated on Day 14. The pre-maxillary was isolated for the immunohistochemical examination. Analysis of Variance (ANOVA) then continued with Tukey Honest Significant Difference (HSD) (p&lt;0.05) was performed to analyze the data. Results: The highest HMGB1 and HSP-70 expression were found in the K+ group pressure&nbsp; side, meanwhile the lowest HMGB1 and HSP-70 expression were found in K- group tension side in the alveolar bone. There was a significant decrease of HMGB1 and HSP-70 expression in T2 compared to T1 and K+ with significant between groups (p&lt;0.05; p=0.0001). Conclusion: The decreased expression of HMGB1 and HSP-70 in alveolar bone of OTM wistar rats due to post administration of GTME that consisted EGCG

Pengaruh Pemberian Epigallocatechin Gallate (Egcg) Terhadap Ekspresi VEGF Dan FGF-2 Pada Pergerakan Gigi Ortodonti Tikus Wistar

Latar Belakang : Pergerakan Gigi Ortodonti (PGO) dapat dicapai melalui remodeling jaringan periodontal yang optimal. Epigallocatechin Gallate (EGCG) dalam daun teh hijau (Camelia Sinensis) yang dikenal untuk meningkatkan aposisi tulang melalui stimulasi Faktor Pertumbuhan. Vascular Endothelial Growth Factor (VEGF) dan Fibroblast Growth factor (FGF-2) memainkan peran penting dalam proses aposisi tulang. Tujuan: Menyelidiki efek pemberian EGCG terhadap ekspresi VEGF dan FGF-2 selama PGO pada tikus wistar. Bahan dan metode: Sampel terdiri dari dua puluh delapan ekor tikus wistar kemudian dibagi menjadi 4 kelompok: kelompok kontrol negatif (K-); kelompok kontrol positif (K +) dengan PGO tetapi tanpa pemberian EGCG; kelompok perlakuan 1 (T1) dengan PGO dan EGCG setelah tujuh hari PGO hingga hari 14; kelompok perlakuan 2 (T2) dengan PGO dan EGCG dari hari 1 hingga hari 14. PGO dipasang dengan menggunakan NiTi close coil spring yang ditempatkan di antara gigi seri dan gigi molar rahang atas untuk memindahkan molar secara mesial dengan kekuatan 10g. Semua sampel dikorbankan pada hari 14 kemudian jaringan rahang diekstraksi untuk analisis imunohistokimia. Data dianalisis untuk menguji perbedaan antara kelompok dengan menggunakan Analisis Varian kemudian dilanjutkan dengan Least Signficant Difference (p <0,05). Hasil: Ekspresi VEGF tertinggi ditemukan pada T2 di kedua area. Sementara itu, ekspresi FGF-2 tertinggi ditemukan di K- di keduaarea. Ada perbedaan yang signifikan dari ekspresi VEGF dan FGF-2 di keduaarea antara kelompok kecuali T1 dan T2. Kesimpulan: Pemberian EGCG dapat meningkatkan ekspresi VEGF dan FGF-2 selama PGO pada tikus wistar