bcl-2 Induction of Urokinase Plasminogen Activator Receptor Expression in Human Cancer Cells through Sp1 Activation INVOLVEMENT OF ERK1/ERK2 ACTIVITY

We have previously demonstrated that Bcl-2 overexpression in human breast carcinoma and melanoma cells synergizes with hypoxia to increase angiogenesis through up-regulation of vascular endothelial growth factor. In this work we demonstrated, for the first time, that Bcl-2 overexpression in cancer cells exposed to hypoxia modulates urokinase plasminogen activator receptor (uPAR) expression through Sp1 transcription factor and that the extracellular signal-regulated kinase (ERK) pathway plays a role in Sp1 transcriptional activity. In particular, an increase in uPAR protein and mRNA expression was found in melanoma bcl-2 transfectants grown under hypoxia when compared with control cells, and a decrease of uPAR protein expression was induced by treatment of cells with specific bcl-2 antisense oligonucleotides. Up-regulation of uPAR expression was accompanied by increased Sp1 protein expression, stability, serine phosphorylation, and DNA binding activity. Treatment of cells with mitramycin A, an inhibitor of Sp1 activity, confirmed the role of Sp1 transcriptional activity in uPAR induction by Bcl-2. The contribution of the ERK pathway in Sp1-increased transcriptional activity was demonstrated by the use of chemical inhibition. In fact, ERK kinase activation was induced in Bcl-2-overexpressing cells exposed to hypoxia, and the ERK kinase inhibitor UO126 was able to down-regulate Sp1 phosphorylation and DNA binding activity. Using a human breast carcinoma line, we obtained data supporting our findings with melanoma cells and identified a link between the induction of Sp1 and uPAR expression as a common bcl-2-controlled phenomenon in human tumors. In conclusion, our results strongly indicate that up-regulation of uPAR expression by Bcl-2 in hypoxia is modulated by Sp1 DNA binding activity through the ERK signaling pathway

Long-Term Performance of a Full-Scale Membrane Plant for Landfill Leachate Pretreatment: A Case Study

This paper presents a case study describing a full-scale membrane bioreactor (MBR) for the pretreatment of landfill leachates. The treatment train includes an aerated equalization tank, a denitrification tank, an oxidation/nitrification tank, and two ultrafiltration units. The plant has worked continuously since 2008 treating landfill leachates at a flux of 2–11 L·h−1·m−2. The old train of membranes worked in these conditions for more than seven years prior to being damaged and replaced. The permeability (K) of the membrane varied between 30 and 80 L·h−1·m−2·bar−1 during the years of operation. In 2010, after two years of operation, the oxidation/nitrification tank was changed to work in alternate cycles of aerated and anoxic conditions, in order to improve the denitrification process. The MBR, working at a mean sludge retention time of 144 days and with mixed liquor suspended solids of 17 g/L, achieved high removal rates of conventional contaminants, with more than 98% for Biochemical Oxygen Demand, 96% for ammonium, and 75% for Chemical Oxygen Demand (COD). From the COD balance, half the COD entering was determined to be biologically oxidized into carbon dioxide, while another 24% remains in the sludge. In order to obtain these results, the company used 5.2 KWh·m−3, while spending 0.79 €·m−3

A Performance Measurement Tool Leading Wastewater Treatment Plants toward Economic Efficiency and Sustainability

Wastewater treatment is an important link in the water cycle that allows for water sanitation and reuse, facilitates energy generation, and allows for the recovery of products from waste. The scientific community has paid significant attention to wastewater treatment, especially from a technical point of view. Extensive literature is available on new technologies, processes, and materials to improve wastewater treatment. However, scant studies have been conducted in the management field focusing on the development of a performance measurement tool that supports plant managers. The current article addresses this literature gap, developing a reporting tool that integrates technical and cost measures and implements it in a large wastewater utility. The tool successfully identifies cause and effect linkages among key plant performance drivers and supports management in finding activities with poor performance and allows them to delay non-relevant measures of control

Effect of Wastewater on the Composition of Bacterial Microbiota of <i>Phragmites australis</i> Used in Constructed Wetlands for Phytodepuration

Phytodepuration occurs in the plant-mediated remediation processes exploited to remove pollutants from wastewater, and Phragmites australis is one of the most used plants. This goal is achieved using constructed wetlands (CW), which are engineered systems designed to mimic the natural processes of pollutants removal. The aim of this work was to characterize the bacterial communities associated to P. australis, soils, and permeates of the CW of Calice (Prato, Italy), to evaluate the possible effect of wastewaters on the CW bacterial communities, through a next-generation sequencing-based approach. A total of 122 samples were collected from different tissues of P. australis (i.e., roots, aerial parts, and stem), soil (i.e., rhizospheric and bulk soil), and permeates, and analyzed. All samples were collected during five sampling campaigns, with the first one performed before the activation of the plant. Obtained results highlighted a specific microbiota of P. australis, conserved among the different plant tissues and during time, showing a lower alpha diversity than the other samples and not influenced by the more complex and variable environmental (soils and permeates) bacterial communities. These data suggest that P. australis is able to select and maintain a defined microbiota, a capacity that could allow the plant to survive in hostile environments, such as that of CW

Biodiversity of Soil Bacterial Communities from the Sasso Fratino Integral Nature Reserve

The Sasso Fratino Integral Nature Reserve (Italy) aims to protect nature and territory. Since no anthropic activities are allowed, it represents a good model to study the bacterial community of a wild environment. The aim of this work was to characterise the cultivable and the total bacterial community of soil samples from the reserve in terms of taxonomy, composition, and structure. Seven soil samples were collected at different altitudes, and the chemical composition, the total and the cultivable microbiota, and the antibiotic resistance profiles of isolates were investigated. Total bacterial communities, studied through Next Generation Sequences analysis, included 390 genera. Samples differed in terms of microbial composition basing on the different altitude/vegetation of collection points. Random Amplified Polymorphic DNA Analysis (RAPD) allowed to identify 82 haplotypes out of 158 bacterial isolates. The taxonomic identification through 16S rDNA sequencing revealed that the strains were affiliated to 21 genera. Antibiotic resistance profiles of bacteria were also investigated, highlighting a high resistance against streptomycin and kanamycin. This work represents the first description of the soil bacterial community from the Natural Reserve of Sasso Fratino, and it is the first study considering the soil microbiota of an Italian integral nature reserve

Progetto di concorso internazionale per la sistemazione del teatro del tempio di Ercole vincitore a Tivoli (Roma)

Il progetto di concorso concerne gli interventi strutturali necessari per l'inserimento di un teatro e di una struttura di accoglienza all'interno del sito del Santuario di Ercole Vincitore a Tivoli (progettisti per gli aspetti strutturali A. Gallo Curcio, F. De Cesaris, F. Iacobelli, S. Barbaliscia). Le gradinate del teatro sono state pensate in modo che costituissero una protezione per i materiali antichi, separati da essi e minimamente alteranti le condizioni di carico (strutture cave), ma nel contempo stabilizzanti. Analogamente si è proceduto per la sistemazione del piazzale. Una struttura lignea smontabile è stata prevista per la scena. Nell'ambito dello stesso progetto è stato previsto il restauro di alcuni volumi, inizialmente dedicati a funzione di accoglienza e attualmente destinati ad Antiquarium. Detti volumi, posizionati marginalmente alla "via tecta" e al teatro, sono stati consolidati con un recupero delle murature in un sistema antisismico ottenuto migliorando i collegamenti murari attraverso la connessione operata con le modifiche e i rinforzi di solai e coperture. Nel caso di un solaio particolarmente robusto è stata prevista l'integrale conservazione con connessioni d'ambito. Analogamente è stato utilizzato il solaio di sostituzione per collegare i muri perimetrali mediante la realizzazione di cordoli metallici sommitali