ABNORMALNI TEST OPTEREĆENJA GLUKOZOM I BLAGI GESTACIJSKI DIJABETES

Objective. The status of carbohydrate metabolism of pregnant women with positive glucose challenge test (GCT), but normal oral glucose tolerance test (OGTT) and their neonates are not defined clearly. Study Design. Pregnant women with normal GCT (n: 120), with abnormal glucose challenge test (AGCT) but normal OGTT (n: 67) and with gestational diabetes (GDM, n: 67) were included into the study. Insulin sensitivity was evaluated by fasting insulin level, homeostasis model assessment of insulin resistance index (HOMA-IR); quantitative insulin check index (QUICKI) and ISOGTT. Serum insulin and glucose values during OGTT were documented. Perinatal outcome and delivery modalities were compared. Results. Both GDM (31.6±5.9 yrs) and AGCT groups (29.0±4.0 yrs) were older than controls (28.1±4.9 yrs). Body mass index (BMI) was the predominant factor affecting both AGCT and GDM groups (OR: 3.78 and 5.97 respectively). Despite there was no significance between insulin indices; serum glucose and insulin values were similarly different; macrosomic infant and caesarean section rates were higher than controls in both GDM and AGCT groups in favor of gestational diabetics (6.6% vs. 18.9%; p=0.0001 and 20% vs. 27.7% p=0.0001 respectively). Conclusion. Pregnant woman with abnormal glucose challenge test have impaired carbohydrate metabolism as in gestational diabetics with a lesser severe degree.Cilj istraživanja. Stanje metabolizma ugljikohidrata u trudnica s pozitivnim testom probira (glucose challenge test – GCT), a normalnim testom opterećenja glukozom (OGTT) te njihove novorođenčadi, nije jasno definirano. Način istraživanja. U studiju su uključene trudnice s normalnim GCT-om (n: 120), s abnormalnim GCT-om ali normalnim OGTT-om (n:67 te trudnice s gestacijskim dijabetesom (n: 67). Insulinska osjetljivost je vrednovana jutarnjiom vrijednošću insulina, modelom prosudbe homeostaze indeksom rezistencije na inzulin (HOMA-IR), kvantitativnim indeksom provjere insulina (QUICKI) i ISOGTT-om. Vrijednosti serumske glukoze i inzulina su analizirane. Uspoređen je perinatalni ishod i način poroda. Rezultati. Trudnice s GDM (31,6±5,9 godina) i one s AGCT (29,0±4,0 godina) su bile starije dobi od kontrolnih trudnica (28,1±4,9 godina). Indeks tjelesne težine (BMI) je bio presudni čimbenik u skupini s AGCT i GDM (OR: 3,78 odnosno 5,97). Unatoč tome nije bilo značajnosti među inzuilinskim indeksima; serumske vrijednosti glukoze i inzulina su bile slično različite; makrosomna djeca i stopa carskih rezova su u trudnica s AGCT i OGTT bile češće, posebice u trudnica s GDM (6,6% prama 18,9%, p = 0,0001; 20% prama 27,7%, p = 0,0001). Zaključak. Trudnice s abnormalnim testom probira na glukozu (AGCT) imaju poremećaj metabolizma ugljikohidrata kao i trudnice s gestacijskim dijabetesom (GDM), ali u nešto manjoj mjeri

ABNORMALNI TEST OPTEREĆENJA GLUKOZOM I BLAGI GESTACIJSKI DIJABETES

Objective. The status of carbohydrate metabolism of pregnant women with positive glucose challenge test (GCT), but normal oral glucose tolerance test (OGTT) and their neonates are not defined clearly. Study Design. Pregnant women with normal GCT (n: 120), with abnormal glucose challenge test (AGCT) but normal OGTT (n: 67) and with gestational diabetes (GDM, n: 67) were included into the study. Insulin sensitivity was evaluated by fasting insulin level, homeostasis model assessment of insulin resistance index (HOMA-IR); quantitative insulin check index (QUICKI) and ISOGTT. Serum insulin and glucose values during OGTT were documented. Perinatal outcome and delivery modalities were compared. Results. Both GDM (31.6±5.9 yrs) and AGCT groups (29.0±4.0 yrs) were older than controls (28.1±4.9 yrs). Body mass index (BMI) was the predominant factor affecting both AGCT and GDM groups (OR: 3.78 and 5.97 respectively). Despite there was no significance between insulin indices; serum glucose and insulin values were similarly different; macrosomic infant and caesarean section rates were higher than controls in both GDM and AGCT groups in favor of gestational diabetics (6.6% vs. 18.9%; p=0.0001 and 20% vs. 27.7% p=0.0001 respectively). Conclusion. Pregnant woman with abnormal glucose challenge test have impaired carbohydrate metabolism as in gestational diabetics with a lesser severe degree.Cilj istraživanja. Stanje metabolizma ugljikohidrata u trudnica s pozitivnim testom probira (glucose challenge test – GCT), a normalnim testom opterećenja glukozom (OGTT) te njihove novorođenčadi, nije jasno definirano. Način istraživanja. U studiju su uključene trudnice s normalnim GCT-om (n: 120), s abnormalnim GCT-om ali normalnim OGTT-om (n:67 te trudnice s gestacijskim dijabetesom (n: 67). Insulinska osjetljivost je vrednovana jutarnjiom vrijednošću insulina, modelom prosudbe homeostaze indeksom rezistencije na inzulin (HOMA-IR), kvantitativnim indeksom provjere insulina (QUICKI) i ISOGTT-om. Vrijednosti serumske glukoze i inzulina su analizirane. Uspoređen je perinatalni ishod i način poroda. Rezultati. Trudnice s GDM (31,6±5,9 godina) i one s AGCT (29,0±4,0 godina) su bile starije dobi od kontrolnih trudnica (28,1±4,9 godina). Indeks tjelesne težine (BMI) je bio presudni čimbenik u skupini s AGCT i GDM (OR: 3,78 odnosno 5,97). Unatoč tome nije bilo značajnosti među inzuilinskim indeksima; serumske vrijednosti glukoze i inzulina su bile slično različite; makrosomna djeca i stopa carskih rezova su u trudnica s AGCT i OGTT bile češće, posebice u trudnica s GDM (6,6% prama 18,9%, p = 0,0001; 20% prama 27,7%, p = 0,0001). Zaključak. Trudnice s abnormalnim testom probira na glukozu (AGCT) imaju poremećaj metabolizma ugljikohidrata kao i trudnice s gestacijskim dijabetesom (GDM), ali u nešto manjoj mjeri

Tumor Necrosis Factor-Alpha Antagonist Administration Recovers Skeletal Muscle Dysfunction in Ovariectomized Rats

Skeletal muscles deteriorate after ovariectomy. Molecular pathway of this deterioration has not been defined. Tumor necrosis factor (TNF)-alpha activation is assumed to trigger muscle atrophy and administration of its antagonist is hypothesized to recover this atrophy in rats. Slow-twitch soleus and fast-twitch extensor digitorum longus muscle functions were investigated in intact, ovariectomized (OVX), and OVX plus 10 mu g/g/week TNF-alpha antagonist administered female rats. Maximum isometric twitch and tetanic contraction responses were lower in the OVX groups. Maximum isometric twitch amplitudes recovered in the extensor digitorum longus but not in the soleus muscles after TNF-alpha antagonist administration. The decrease in responses to tetanic stimulations recovered in the OVX TNF group at frequencies higher than 20 Hz in both muscle types. OVX animals body weight was 21% higher than intact animals. Muscle weight to body weight ratios of the OVX groups were higher than the control group which recovered after TNF-alpha antagonist administration. Findings suggest that the functional loss in OVX rat muscles is TNF-alpha pathway dependent. Skeletal muscle atrophy and function after OVX recovered by TNF-alpha antagonist administration. (C) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J. Orthop, Res. 29: 275-280, 201

Treatment Of Implant-Related Methicillin-Resistant Staphylococcus Aureus Osteomyelitis With Vancomycin-Loaded Vk100 Silicone Cement: An Experimental Study In Rats

Introduction: The purpose of this present study is to investigate the efficacy of vancomycin-loaded VK100 silicone cement drug delivery system in the treatment of implant-related methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis in rats. Materials and Methods: Thirty-six adult (18-20 weeks old) female Sprague-Dawley rats were included in the study. All rats underwent experimental osteomyelitis surgery via injecting 100 mu L bacterial suspension of MRSA into the medullary canal. After a 2-week duration for the formation of osteomyelitis model, rats were assigned Introduction: randomly into four groups: control (C), systemic vancomycin (V), local vancomycin-loaded VK100 silicone cement (vVK100), and systemic vancomycin and local vancomycin-loaded VK100 silicone cement (V+vVK100). The following treatment protocols were administered to each group for 4 weeks. For group C, 0.9% saline solution equivalent to the volume of vancomycin dose (approximately 1 ml/kg) was administered intraperitoneally twice daily (12-h intervals). For group V, 15 mg/kg of vancomycin was administered intraperitoneally twice daily (12-h intervals). For group vVK100, vVK100 polymer was included so that the intramedullary canal of the rats are affected. For group V+vVK100, vVK100 polymer was included so that the intramedullary canal of the rats are affected and 15 mg/kg of vancomycin was administered intraperitoneally twice daily (12-h intervals). After 4 weeks of treatment, clinical, radiologic, microbiologic, and histopathologic evaluations were performed for all groups. Results: Results of this study revealed that all scores of the evaluation criteria for the treatment groups (groups V, vVK100, and V+vVK100) decreased due to the treatment protocols when compared to group C. These results show the effectiveness of all treatment protocols for the implant-related chronic MRSA osteomyelitis. However, there were no statistical difference between these three protocols. Conclusions: vVK100 polymer, as a local antibiotic delivery system, seems to be an effective method for the treatment of implant-related chronic MRSA osteomyelitis.WoSScopu