A Comparative Study of Commercial Modified Celluloses as Bread Making Additives

The effect of commercial modified celluloses: microcrystalline cellulose, carboxymethyl cellulose, and hydroxypropylmethyl cellulose on bread quality attributes and their potential protective effect with respect to bread staling were analyzed. Two levels of gums were assayed (0.5 and 1.5 g/100 g flour). The best performance was obtained with carboxymethyl cellulose and hydroxypropylmethyl cellulose F 4 M at both levels; these gums led to higher specific volumes and a better crumb texture as measured by texture profile analysis. In general, crumbs were softer, more cohesive, and resilient and exhibited lower chewiness values. Other gums like microcrystalline cellulose and hydroxypropylmethyl cellulose F50 did not improve bread quality on the same extent. Mechanical spectra obtained by dynamic mechanical analysis assays indicated a marked change in molecular mobility when carboxymethyl cellulose was present. Bread staling was evaluated by texture profile analysis, moisture loss, and calorimetric assays. Gums did not avoid retrogradation and even exhibited an accelerating effect, probably due to changes in water retention and migration during storage. However, in most cases, final crumb hardness in samples with hydrocolloids was lower than that in the control sample.Centro de Investigación y Desarrollo en Criotecnología de Alimento

Interactive activities to stimulate debate and critical thinking about issues related to Earth sciences and sustainable development

During the International Year of Planet Earth (2007-2009), the Department of Earth Sciences of Turin University and a local Museum of Natural History promoted a project entitled, Understanding how the Earth works: from local situations to global processes. In this context, two geothematic exhibitions on the Cape Verde Archipelago were designed and staged in local museums. The exhibition called Getting to know a volcano in order to live with it was the subject of action research that involved the design of interactive activities and the analysis of data collected during guided tours conducted with students of different ages. This study allowed the demonstration of the effectiveness of teaching strategies in which relevant Earth sciences topics are proposed, like risk and sustainable development, thus stimulating debate among the students. This approach enhances the cultural experience of individuals by sharing it with other people. The aim was to widen their awareness of the cultural value of the territory, and to stimulate a new critical way of thinking about the Earth sciences. These didactic tools were further developed when they were proposed and pursued by experienced museum guides and teachers, who were able to involve not only institutions (museums and schools) in the knowledge construction process, but also families, relatives and the local community

Proinflammatory response of canine trophoblasts to Brucella canis infection

Brucella canis infection is an important cause of late-term abortion in pregnant bitches. The pathophysiological mechanisms leading to B. canis–induced abortion are unknown, but heavily infected trophoblasts are consistently observed. As trophoblasts responses to other pathogens contribute to placental inflammation leading to abortion, the aim of the present study was to characterize the cytokine response of canine trophoblasts to B. canis infection. To achieve this, trophoblasts isolated from term placenta of healthy female dogs were infected with B. canis, culture supernatants were harvested for cytokine determinations, and the load of intracellular viable B. canis was determined at different times post-infection. Additionally, cytokine responses were assessed in non-infected trophoblasts stimulated with conditioned media (CM) from B. canis-infected canine monocytes and neutrophils. Finally, cytokine response and bacteria replication were assessed in canine placental explants infected ex vivo. B. canis successfully infected and replicated in primary canine trophoblasts, eliciting an increase in IL-8 and RANTES (CCL5) secretion. Moreover, the stimulation of trophoblasts with CM from B. canis-infected monocytes and neutrophils induced a significant increase in IL-8, IL-6 and RANTES secretion. B. canis replication was confirmed in infected placental explants and the infection elicited an increased secretion of TNF-α, IL-8, IL-6 and RANTES. This study shows that canine trophoblasts produce proinflammatory cytokines in response to B. canis infection and/or to stimulation with factors produced by infected monocytes and neutrophils. These cytokines may contribute to placental inflammation leading to abortion in B. canis-infected pregnant bitches.Fil: Fernandez, Andrea Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Hielpos, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Ferrero, Mariana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Fossati, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Baldi, Pablo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentin

Rheological and Microstructure Characterization of Composite Dough with Wheat and Mesquite (Prosopis spp) Flours

Rheological behavior and microstructural characteristics of composite dough with wheat and mesquite flours (from Prosopis alba) were analyzed by response surface methodology. A central composite design was applied varying proportions of water (50 to 80 g each 100 g wheat flour) and mesquite flour (0 to 70 each 100 g wheat flour). Texture profile analysis showed that increasing the amount of mesquite flour and decreasing the amount of water led to less cohesive and more resilient doughs. Stress relaxation curves were fitted with a Maxwell model and relaxation times were obtained. These parameters resulted higher for those formulations low in mesquite content and high water levels. Composite dough showed a typical viscoelastic behavior with higher elastic moduli (G) when mesquite flour ratio in the mixture was increased. 1H-RMN T2 relaxation assays revealed higher mobility in samples with high amounts of water and minimum level of mesquite. A farinograph was adequate to obtain optimum water amounts and showed that addition of mesquite led to less stable dough respect to control wheat dough. By using confocal laser scanning microscopy with fluorescent probes rhodamine B and fluoresceine isothiocyanate, a poor gluten network development or protein aggregation was observed when water contents were far from optimum.Fil: Bigne, Facundo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Puppo, Maria Cecilia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Ferrero, Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentin

Outdoor thermal and visual perception of natural cool materials for roof and urban paving

Given the acknowledged thermal performance of natural light color gravels applied as cool roof and cool urban paving, this work is aimed at investigating if such behavior is perceivable by pedestrians, who are questioned in this paper about their visual and thermal comfort perception. In fact, there are still related aspects to analyze, in order to optimize their application and provide a comfortable space for users, both on the thermal and the visual point of view. Therefore, the question that this work wants to answer is: given their intrinsic characteristics, do these materials create a sensitive thermally and visually more comfortable environment for pedestrians? In order to address this uninvestigated issue, users’ judgment about visual and thermal comfort of these surfaces is considered, also by comparing them with grassland and asphalt. Also, the statistical correspondence between physical properties of such materials and possible correspondence with respect to human perception with varying weather conditions is analyzed. Given the relatively high reflectance of these materials, it appears particularly important to evaluate these aspects, to consciously apply them as urban paving or roof covering by optimizing their natural passive cooling potential. In this preliminary study, users’ response to these surfaces is evaluated by mean of field surveys, both on the thermal and the visual evaluation, and contemporary in-field measurements of surface parameters. Also, human perception with respect to these high-reflectance surfaces’ is compared with the one related to grassland and asphalt, with varying weather conditions. Then, a statistical analysis is performed to investigate the differences among different gravels, grassland and asphalt, based on surveys’ results. The results show how pedestrians, questioned during summer days, prefer grassland, while asphalt is the less favorite surface both visually and thermally; there is a small difference between gravels’ types evaluation, while weather variability affect the preferences

Magnetic Field Effects on Molecular Emissivity in Solutions

Control over dynamics of excited states of molecules is fundamental to utilization of these states in all areas of technologies, including optical microscopy and tomography. We explored the possibility of magnetically controlling molecular emissivity by influencing spin dynamics in radical pairs (RPs) and triplet-triplet pair. We envisioned that by including RPs into a pathway leading to the formation (or decay) of an emissive triplet state, magnetic influence on phosphorescence could be realized via modulation of the RP\u27s spin dynamics. RPs can initially be produced in their singlet or triplet spin state. These two cases were explored in the studies of electron and energy dynamics in series of donor-acceptor systems, comprising phosphorescent platinum (II) porphyrins (PtP) and rhodamine B (RhB+) derivatives. In one series, the phosphorescent triplet state of PtP is generated by way of photo-excitation of RhB+, followed by photoinduced electron transfer with formation of a singlet-born RP, RP intersystem crossing, and subsequent recombination of the triplet RP. Similarly, singlet-born RPs were included into a pathway leading to PtP triplet state in a triad system comprising PtP, anthracene, and boron dipyrromethene. Using another series, we have demonstrated that visible room-temperature phosphorescence can be modulated by weak magnetic fields (\u3c1T). In this case, the RP is initially born in its triplet state upon direct excitation of PtP, followed by electron transfer originating in the PtP triplet state. External magnetic field modulates spin dynamics in the RP, affecting contribution of the singlet charge recombination channel and thereby influencing the phosphorescence. Spin dynamics of triplet-triplet pair is also susceptible to magnetic field. Triplet-triplet pair can undergo triplet-triplet annihilation (TTA) process leading to photon upconversion, which is typically observed as p-type delayed fluorescence. We have demonstrated TTA-sensitized delayed fluorescence and delayed phosphorescence, mediated by near-infrared absorbing metalloporhyrins as sensitizers in solution at room temperature, can be magnetically modulated. These studies present unusual and interesting examples of magnetic field effects on molecular emission and set the stage for rational design of optical imaging probes with magnetically controlled emissivity

Propiedades psicométricas del inventario de creencias sobre violencia de pareja íntima

This study analysed the psychometric properties of the Inventory of Beliefs about Intimate Partner Violence (IBIPV) which is designed to measure general and specific attitudes to violence against women in intimate relationships. The participants were 1169 people aged from 18 to 77 years. We found that the IBIPV has a three-factor structure, the factors identified were: a) Justifying Partner Violence (JPV), b) Victims Responsible for Violence (VRV) and c) Abuser Responsible for Violence (ARV). Multi-group confirmatory factor analysis showed three levels of measurement invariance across gender: configural, metric and scalar invariance. As expected, evidence of the convergent and divergent validity of the inventory was provided by comparisons with the Ambivalent Sexism Inventory (ASI) and Ambivalence toward Men Inventory (AMI). Potential applications of the scale in the field of partner violence are discussed.  Este estudio analiza las propiedades psicométricas del Inventa-rio de Creencias sobre la violencia de pareja. En concreto, este inventario mide actitudes generales y específicas hacia la violencia contra las mujeres dentro de las relaciones de pareja. En el estudio participan 1169 personas con una edad comprendida entre 18 y 77 años. Los resultados muestran tres factores: a) Justificación de la violencia de género, b) Responsabilidad de la víctima y c) Responsabilidad del maltratador. El análisis factorial con-firmatorio mostró índices de ajuste apropiados: CFI = .952, NFI = .942 y RMSEA = .062 (90% CI [.058 - .065]). Además, los resultados muestran validez convergente y divergente con el sexismo ambivalente hacia las mu-jeres y hacia los hombres. Se discute la utilidad de esta escala en el ámbito de la violencia contra la pareja.

Btp Proteins from Brucella abortus Modulate the Lung Innate Immune Response to Infection by the Respiratory Route

Although inhalation of infected aerosols is a frequent route for Brucella infection in humans, it rarely causes pulmonary clinical manifestations, suggesting a mild or nearly absent local inflammatory response. The goal of this study was to characterize the early innate immune response to intratracheal infection with Brucella abortus in mice and to evaluate whether it is modulated by this pathogen. After infection with 106 CFU of B. abortus, the pulmonary bacterial burden at 7 days post-infection (p.i.) was comparable to the initial inoculum, despite an initial transient decline. Brucella was detected in spleen and liver as early as 1 day p.i. IL-1β and MCP-1 increased at 3 days p.i., whereas IL-12, KC, TNF-α, and IFN-γ only increased at 7 days p.i. Histological examination did not reveal peribronchial or perivascular infiltrates in infected mice. Experiments were conducted to evaluate if the limited inflammatory lung response to B. abortusis caused by a bacterial mechanism of TLR signaling inhibition. Whereas inoculation of E. coli LPS to control mice [phosphate-buffered saline (PBS)/LPS] caused lung inflammation, almost no histological changes were observed in mice preinfected intratracheally with B. abortus (WT/LPS). We speculated that the Brucella TIR-containing proteins (Btps) A and B, which impair TLR signaling in vitro, may be involved in this modulation. After LPS challenge, mice preinfected with the B. abortus btpAbtpB double mutant exhibited a stronger pulmonary polymorphonuclear infiltrate than WT/LPS mice, although milder than that of the PBS/LPS group. In addition, lungs from B. abortus btpAbtpB-infected mice presented a stronger inflammatory infiltrate than those infected with the WT strain, and at day 7 p.i., the pulmonary levels of KC, MCP-1, and IL-12 were higher in mice infected with the mutant. This study shows that B. abortus infection produces a mild proinflammatory response in murine lungs, partially due to immune modulation by its Btp proteins. This may facilitate its survival and dissemination to peripheral organs.Fil: Hielpos, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Ferrero, Mariana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Fernandez, Andrea Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Falivene, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Comerci, Diego José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Baldi, Pablo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentin

Thermal comfort in the historical urban canyon: the effect of innovative materials

Urban heat island (UHI) can considerably affect the thermal quality of the urban environment, especially within urban canyons, that have typically low sky view factor and limited surface heat re-emission capability. A huge research effort has been registered to develop mitigation solutions for UHI, such as cool materials and greenery. Nevertheless, it is not always possible to apply such strategies in historical urban environments due to constrains for the preservation of their cultural value that do not allow to modify the exterior architectural appearance of heritage buildings. In this scenario, the present paper deals with the analysis of the potential of innovative cool materials characterized by the same appearance of historical ones in mitigating the UHI occurring in the context of a historical urban canyon located in central Italy selected as pilot case study. To this purpose, a preliminary experimental characterization of such innovative highly reflective materials has been performed. Afterwards, an experimental continuous monitoring campaign of the main outdoor microclimate parameters and a numerical modelling of the canyon have been carried out to evaluate the local mitigation capability of such materials when applied over the vertical and horizontal surfaces of the historical canyon. The results show the huge potential of the proposed innovative cool materials in mitigating the local microclimate of the historical urban canyon. In fact, a MOCI reduction up to 0.15 and 0.30 is detected by applying cool red envelope materials and cool red envelope materials plus cool grey paving materials, respectively, on the canyon surfaces