Doppler ultrasonography of the pectinis oculi artery in harpy eagles (Harpia harpyja)

Twenty harpy eagles (Harpia harpyja) without systemic or ocular diseases were examined to measure blood velocity parameters of the pectinis oculi artery using Doppler ultrasonography. Pectinate artery resistive index (RI) and pulsatility index (PI) were investigated using ocular Doppler ultrasonography. The mean RI and PI values across all eyes were 0.44±0.10 and 0.62±0.20 respectively. Low RI and PI values found in the harpy eagle´s pectinis oculi artery compared with the American pekin ducks one and other tissue suggest indeed a high metabolic activity in pecten oculi and corroborates the hypothesis of a nutritional function and/or intraocular pressure regulation.Keywords: Avian posterior segment, Pulsatility index, Raptors, Resistive index

Doppler ultrasonography of the pectinis oculi artery in harpy eagles (Harpia harpyja)

Twenty harpy eagles (Harpia harpyja) without systemic or ocular diseases were examined to measure blood velocity parameters of the pectinis oculi artery using Doppler ultrasonography. Pectinate artery resistive index (RI) and pulsatility index (PI) were investigated using ocular Doppler ultrasonography. The mean RI and PI values across all eyes were 0.44±0.10 and 0.62±0.20 respectively. Low RI and PI values found in the harpy eagle´s pectinis oculi artery compared with the American pekin ducks one and other tissue suggest indeed a high metabolic activity in pecten oculi and corroborates the hypothesis of a nutritional function and/or intraocular pressure regulation.Keywords: Avian posterior segment, Pulsatility index, Raptors, Resistive index

Radiotherapy modulates expression of EGFR, ERCC1 and p53 in cervical cancer

<div><p>Cervical cancer is a public health problem and the molecular mechanisms underlying radioresistance are still poorly understood. Here, we evaluated the modulation of key molecules involved in cell proliferation, cell cycle and DNA repair in cervical cancer cell lines (CASKI and C33A) and in malignant tissues biopsied from 10 patients before and after radiotherapy. The expression patterns of epidermal growth factor receptor (EGFR), excision repair cross-complementation group 1 (ERCC1) and p53 were evaluated in cancer cell lines by quantitative PCR and western blotting, and in human malignant tissues by immunohistochemistry. The mutation status of TP53 gene was evaluated by direct sequencing. Among cell lines, absent or weak modulations of EGFR, ERCC1 and p53 were observed after exposure to 1.8 Gy. Conversely, increased expressions of p53 (5/10 patients; P=0.0239), ERCC1 (5/10 patients; P=0.0294) and EGFR (4/10 patients; P=0.1773) were observed in malignant tissues after radiotherapy with the same radiation dose. TP53 mutations were found only in one patient. Here we show that a single dose of radiotherapy induced EGFR, ERCC1 and p53 expression in malignant tissues from cervical cancer patients but not in cancer cell lines, highlighting the gap between in vitro and in vivo experimental models. Studies on larger patient cohorts are needed to allow an interpretation that an upregulation of p53, EGFR and ERCC1 may be part of a radioresistance mechanism.</p></div

Desempenho animal e avaliação do potencial produtivo de forragens para ensilagem, por intermédio de diferentes fontes de suplementação nitrogenada Animal performance and evaluation of forage potential production for silage making by means of different sources of nitrogen supplementation

Este estudo foi conduzido para avaliar o milho (Zea mays L.) AG 1051 e os sorgos (Sorghum bicolor, (L.) Moench) AG 2006 e BR 303, para produção de silagem, e verificar o efeito da substituição do farelo de soja por uréia (0, 50 e 100%), nas rações concentradas para bovinos confinados. As silagens foram usadas em dois experimentos e os animais foram distribuídos em delineamento experimental inteiramente casualizado. No primeiro, 72 bovinos mestiços F1 Pardo Suíço x Nelore, inteiros, com 20 meses de idade e 412 kg PV, foram distribuídos em arranjo fatorial 3 x 3 (silagem de milho e de sorgos e nível de substituição do farelo de soja por uréia). No segundo, 48 novilhos Nelore, com 34 meses e 378 kg PV, foram distribuídos em arranjo fatorial 2 x 3 (silagem de sorgos e nível de substituição do farelo de soja por uréia). Não houve diferença entre as forragens quanto às produções/ha de matéria seca total e de folha, colmo e à relação espiga/panícula. As plantas de milho e sorgo BR 303 apresentaram cerca de 53% de espiga/panícula quando comparado a 45% do sorgo AG 2006. Não houve interação entre as silagens e a fonte de nitrogênio. A silagem de milho propiciou melhor desempenho para os animais mestiços. Economicamente, o melhor resultado foi obtido com o uso da silagem de milho e do farelo de soja. O desempenho dos animais Nelores não foi influenciado pelo tipo de silagem e pela fonte de nitrogênio. Não houve diferença no retorno econômico para as silagens. Contudo, para as fontes de nitrogênio, o maior retorno economico foi obtido com o uso da uréia, seguido da mistura farelo de soja + uréia e, por último, com o farelo de soja.<br>This study was conducted to evaluate the corn (Zea mays L.) AG 1051 and the sorghums (Sorghum bicolor (L.) Moench) AG 2006 and BR 303, for silage production, and the effect of substitution of soybean meal by urea (0,50 and 100%) in the concentrated diets for feedlot cattle. The silages were used in two experiments and the animals were alloted to a completely experimental design. In the first, 72 F1 crossbred Brown Swiss x Nellore bulls, with 20 months of age and 412 kg LW were distributed to a 3x3 factorial arrangement (corn silage and sorghums silage and level of substitution of soybean meal by urea). In the second, 48 Nellore steers, with 34 month of age and 378 kg LW, were allotted to a 2 x 3 factorial arrangement (sorghum silage and level of substitution of soybean by urea). There were no differences between the forages as for production/ha of total dry matter and leaves, stem and of ear/panicle ratio. The corn and sorghum BR 303 plants presented about 53% of ear/panicle ratio as compared to 45% of the sorghum AG 2006. There was no interaction between the silage and the nitrogen source. The corn silage provided better performance for the crossbred bulls. Economically, the highest profit was obtained by using corn silage and soybean meal. The performance of Nellore steers was not affected by silage type or nitrogen source. There were no differences in the economic return for the silages. However, for the nitrogen source, the higher economic return was obtained by the use of urea followed by soybean meal + urea mixture and, finally, by soybean meal