Agri-food surplus, waste and loss as sustainable biobased ingredients: a review

Ensuring a sustainable supply of food for the world’s fast growing population is a major challenge in today’s economy, as modern lifestyle and increasing consumer concern with maintaining a balanced and nutritious diet is an important challenge for the agricultural sector worldwide. This market niche for healthier products, especially fruits and vegetables, has increased their production, consequently resulting in increased amounts of agri-food surplus, waste, and loss (SWL) generated during crop production, transportation, storage, and processing. Although many of these materials are not utilized, negatively affecting the environmental, economic, and social segments, they are a rich source of valuable compounds that could be used for different purposes, thus preventing the losses of natural resources and boosting a circular economy. This review aimed to give insights on the efficient management of agri-food SWL, considering conventional and emerging recovery and reuse techniques. Particularly, we explored and summarized the chemical composition of three worldwide cultivated and consumed vegetables (carrots, broccoli and lettuce) and evaluate the potential of their residues as a sustainable alternative for extracting value-added ingredients for the development of new biodynamic productsThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through the national funds FCT/MCTES to CIMO (UIDB/00690/2020) and national funding by FCT, P.I., through the institutional scientific employment program contract for L. Barros and A. Fernandes.info:eu-repo/semantics/publishedVersio

Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrancosa) leaves.

We report the determination of phenolic compounds in olive leaves by reversedphase HPLC/DAD, and the evaluation of their in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely Gram positive (Bacillus cereus, B. subtilis and Staphylococcus aureus), Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae) and fungi (Candida albicans and Cryptococcus neoformans). Seven phenolic compounds were identified and quantified: caffeic acid, verbascoside, oleuropein, luteolin 7-O-glucoside, rutin, apigenin 7-O-glucoside and luteolin 4’-O-glucoside. At low concentrations olive leafs extracts showed an unusual combined antibacterial and antifungal action, which suggest their great potential as nutraceuticals, particulalry as a source of phenolic compounds

Phenolics and antimicrobial activity of traditional stoned table olives alcaparra

In the present work, we report the determination of phenolic compounds in ‘alcaparra’ table olives by reversed-phase HPLC/DAD, and the evaluation of their extract in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely Gram positive (Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus), Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae) and fungi (Candida albicans and Cryptococcus neoformans). Three flavonoidic compounds were identified and quantified: luteolin 7-O-glucoside, apigenin 7-O-glucoside, and luteolin. At low concentrations (0.05 mg/mL) ‘alcaparra’ extract revealed significant inhibition of both Gram positive and Gram negative bacteria growth, with exception of P. aeruginosa. Nevertheless, no antifungal activity was observed at the tested concentrations

Hazel (Corylus avellana L.) leaves as source of antimicrobial and antioxidative compounds

Aqueous extracts of leaves of different hazel (Corylus avellana L.) cultivars (Cv. M. Bollwiller, Fertille de Coutard and Daviana), were analysed by reversed-phase HPLC/DAD for the definition of their phenolic composition. Antioxidant potential was assessed by the reducing power assay, and the scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and b-carotene linoleate model system. Their antimicrobial capacity was also tested against Gram positive (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae) and fungi (Candida albicans, Cryptococcus neoformans). Eight phenolic compounds were identified: 3-, 4- and 5-caffeoylquinic acids, caffeoyltartaric acid, p-coumaroyltartaric acid, myricetin-rhamnoside, quercetin 3-rhamnoside and kaempferol 3-rhamnoside. A p-coumaric acid, three myricetin and one quercetin derivatives were also detected. The hazel leaves extract presented high antioxidant activity in a concentration-dependent way, in general with similar behaviour of all cultivars. Gram positive bacteria revealed to be very sensitive to hazel leaf extract (MIC 0.1 mg/ml for B. cereus and S. aureus and 1 mg/ml for B. subtilis). However, Gram negative and the fungi displayed much lower sensitivity, being P. aeruginosa and C. albicans resistant at 100 mg/ml. Cv. M. Bollwiller exhibited the most potent antimicrobial activity

Walnut (Juglans regia L.) leaves: phenolic compounds, antimicrobial activity ans antioxidant potential of different cultivars

Different cultivars of walnut (Juglans regia L.) leaves (Cv. Lara, Franquette, Mayette, Marbot, Mellanaise and Parisienne) grown in Portugal, were investigated in what concerns phenolic compounds and antimicrobial and antioxidant properties. Phenolics analysis was performed by reversed-phase HPLC/DAD and 10 compounds were identified and quantified: 3- and 5-caffeoylquinic acids, 3- and 4-pcoumaroylquinic acids, p-coumaric acid, quercetin 3-galactoside, quercetin 3-pentoside derivative, quercetin 3-arabinoside, quercetin 3-xyloside and quercetin 3-rhamnoside. The antimicrobial capacity was screened against Gram positive (Bacillus cereus, B. subtilis, Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae) and fungi (Candida albicans, Cryptococcus neoformans). Walnut leaves selectively inhibited the growth of Gram positive bacteria, being B. cereus the most susceptible one (MIC 0.1 mg/mL). Gram negative bacteria and fungi were resistant to the extracts at 100 mg/mL. Lara walnut leaves were also submitted to antibacterial assays using 18 clinical isolates of Staphylococcus sp. Antioxidant activity was accessed by the reducing power assay, the scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and b-carotene linoleate model system. In a general way, all of the studied walnut leaves cultivars presented high antioxidant activity (EC50 values lower than 1 mg/mL), being Cv. Lara the most effective one

Phenolic compounds and bioactive properties of Ruscus aculeatus L. (asparagaceae): the pharmacological potential of an underexploited subshrub

Ruscus aculeatus L. is a subshrub used in traditional medicine in different parts of the world, namely in Europe and the Iberian Peninsula. According to reported folk knowledge, the aerial parts are mainly used as diuretics and the underground organs are used for the treatment of disorders of the urinary system and as a laxative. In this work, the aerial part and the roots and rhizomes of R. aculeatus were chemically characterized with regard to the content of phenolic compounds and bioactive properties. Aqueous (infusions and decoctions) preparations and hydroethanolic extracts from the two mentioned parts of the plant were prepared. Nine phenolic compounds were detected in all the extracts. Apigenin-C-hexoside-C-pentoside isomer II was the major compound in aqueous extracts and, in the hydroethanolic extract was quercetin-O-deoxyhexoside-hexoside followed by apigenin-C-hexoside-C-pentoside isomer II. All extracts revealed antioxidant activity and potential to inhibit some of the assayed bacteria; aqueous extracts of the aerial part and infusions of roots and rhizomes did not show cytotoxic effects on a non-tumor primary cell culture. This preliminary study provides suggestions of the biological potential associated with the empirical uses and knowledge of this species, in particular its bioactivities.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through the national funds FCT/MCTES to CIMO (UIDB/00690/2020) and national funding by FCT, P.I., through the institutional scientific employment program contract for L. Barros, A. Fernandes, M.I. Dias, C. Pereira, and R.C. Calhelha. The authors are also grateful to FEDER-Interreg España-Portugal program for financial support through the project TRANSCoLAB 0612_TRANS_CO_LAB_2_P and to European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project Norte-01-0145-FEDER- 000042: GreenHealth.info:eu-repo/semantics/publishedVersio

Bioactive properties of Ruscus aculeatus L.: na underexploited subshrub

Ruscus aculeatus L. is a sub-shrub used in traditional medicine in different parts of the world, namely in Europe and the Iberian Peninsula[1]. Traditionally, the aerial parts are mainly used as diuretics and the underground parts are used for the treatment of urinary tract diseases and as a laxative[2]. In the present work, the aerial part, roots, and rhizomes of R. aculeatus were characterized regarding their bioactive properties in order to contribute to the knowledge of the chemical composition of this unexplored species. The plant material of R. aculeatus was harvested in April 2019 inside woodlands and hedgerows, in Valpaços, Portugal. Two distinct parts were gathered: the aerial part (cladodes or laminar stems and lateral branches) and the underground organs (rhizomes with roots). Hydroethanolic extracts and aqueous (infusions and decoctions) preparations from the two mentioned parts of the plant were prepared. Multi-resistant clinical bacterial strains (Enterococcus faecalis, Listeria monocytogenes, methicillin-resistant Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Morganela morganii, Proteus mirabilis and Pseudomonas aeruginosa) were used to screen the antimicrobial activity and the antioxidant activity was evaluated through two different in vitro assays: inhibition of lipid peroxidation in brain homogenates by thiobarbituric acid reactive substances (TBARS) and oxidative hemolysis inhibition assay (OxHLIA). The cytotoxic activity was assayed using MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung carcinoma), HeLa (cervical carcinoma) and HepG2 (hepatocellular carcinoma) human tumor cell lines, and also a non-tumor cell line (porcine liver primary cells, PLP2). All extracts revealed antioxidant activity and potential to inhibit some of the assessed bacteria; except for the infusion of the aerial part (for the lines MCF7 and HepG2) and decoction extracts (line MCF7), all the remaining extracts presented effective results in the inhibition of the tested cell lines; the aqueous extracts of the aerial part and infusions of roots and rhizomes did not show cytotoxic effects in a non-tumor primary cell culture. This preliminary study provided innovative and interesting results in relation to the bioactive properties of this little-studied and explored wild plant.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020) and national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros, A. Fernandes, C. Pereira and R.C. Calhelha´s contract. The authors are grateful to the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project Norte-01-0145-FEDER-000042: GreenHealth.info:eu-repo/semantics/publishedVersio

Role of dynamical particle-vibration coupling in reconciliation of the d3/2d_{3/2} puzzle for spherical proton emitters

It has been observed that decay rate for proton emission from $d_{3/2}$ single particle state is systematically quenched compared with the prediction of a one dimensional potential model although the same model successfully accounts for measured decay rates from $s_{1/2}$ and $h_{11/2}$ states. We reconcile this discrepancy by solving coupled-channels equations, taking into account couplings between the proton motion and vibrational excitations of a daughter nucleus. We apply the formalism to proton emitting nuclei $^{160,161}$Re to show that there is a certain range of parameter set of the excitation energy and the dynamical deformation parameter for the quadrupole phonon excitation which reproduces simultaneously the experimental decay rates from the 2$d_{3/2}$, 3$s_{1/2}$ and 1$h_{11/2}$ states in these nuclei.Comment: RevTex, 12 pages, 4 eps figure

SCI1 Is a Direct Target of AGAMOUS and WUSCHEL and Is Specifically Expressed in the Floral Meristematic Cells

The specified floral meristem will develop a pre-established number of floral organs and, thus, terminate the floral meristematic cells. The floral meristematic pool of cells is controlled, among some others, by WUSCHEL (WUS) and AGAMOUS (AG) transcription factors (TFs). Here, we demonstrate that the SCI1 (Stigma/style cell-cycle inhibitor 1) gene, a cell proliferation regulator, starts to be expressed since the floral meristem specification of Nicotiana tabacum and is expressed in all floral meristematic cells. Its expression is higher in the floral meristem and the organs being specified, and then it decreases from outside to inside whorls when the organs are differentiating. SCI1 is co-expressed with N. tabacum WUSCHEL (NtWUS) in the floral meristem and the whorl primordia at very early developmental stages. Later in development, SCI1 is co-expressed with NAG1 (N. tabacum AG) in the floral meristem and specialized tissues of the pistil. In silico analyses identified cis-regulatory elements for these TFs in the SCI1 genomic sequence. Yeast one-hybrid and electrophoresis mobility shift assay demonstrated that both TFs interact with the SCI1 promoter sequence. Additionally, the luciferase activity assay showed that NAG1 clearly activates SCI1 expression, while NtWUS could not do so. Taken together, our results suggest that during floral development, the spatiotemporal regulation of SCI1 by NtWUS and NAG1 may result in the maintenance or termination of proliferative cells in the floral meristem, respectively