QTL mapping of soybean cyst nematode race 9: a generalized linear modeling approach

The Female Index (FI) is a relative measure of host suitability of a soybean line for a particular nematode population and often shows a non-normal distribution. Moreover, most quantitative trait loci (QTL) mapping methods assume that the phenotype follows a normal distribution such as composite interval mapping (CIM). Therefore, a generalized linear modeling (GLM) approach was employed to map QTL for resistance to race 9 of the soybean cyst nematode (SCN) using a total of 83 simple sequence repeat markers (SSR). Two GLM models were tested: model 1, where the FI was treated as a continuous variable, assuming a Gamma distribution with a logarithmic link function; and model 2, where the FI was treated as a categorical trait in a five-item hierarchy, assuming a multinomial distribution with a cumulative logit link function. The FI data of 108 recombinant inbred lines (RIL) confirmed the non-normal distribution for race 9 of the SCN (Shapiro-Wilk?s w=0.86, P<0.0001, skewness=1.52 and kurtosis=2.93). Eight RIL were confirmed to be resistant (FI≤10), and 23 to be highly susceptible (FI≥100). Both GLM models identified one QTL for SCN on the molecular linkage group G, between the markers Satt275 and Satt038 at 48.4 centiMorgans (P=0.017 and 0.033, for models 1 and 2, respectively). Additionally, these results were also compared with the CIM and Bayesian interval mapping (BIM) methods, assuming experimental data with a non-normal response, to determine the robustness and statistical power of these two methods for mapping QTLs. The results make clear that generalized linear modeling approach can be used as an efficient method to map QTLs in a continuous trait with a non-Gaussian distribution. CIM and BIM were robust enough for a reliable mapping of QTLs underlying nonnormally distributed data.Fil: Arriagada, Osvin. Universidad de Talca; ChileFil: Ferreira, Marcia F. S.. Universidade Federal Do Espirito Santo; BrasilFil: Cervigni, Gerardo Domingo Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Schuster, Ivan. Central Cooperative for Agricultural Research; BrasilFil: Scapim, Carlos A.. Universidade Estadual de Maringá; BrasilFil: Mora, Freddy. Universidad de Talca; Chil

Neuroanatomical pattern classification in a population-based sample of first-episode schizophrenia

AbstractRecent neuroanatomical pattern classification studies have attempted to individually classify cases with psychotic disorders using morphometric MRI data in an automated fashion. However, this approach has not been tested in population-based samples, in which variable patterns of comorbidity and disease course are typically found. We aimed to evaluate the diagnostic accuracy (DA) of the above technique to discriminate between incident cases of first-episode schizophrenia identified in a circumscribed geographical region over a limited period of time, in comparison with next-door healthy controls. Sixty-two cases of first-episode schizophrenia or schizophreniform disorder and 62 age, gender and educationally-matched controls underwent 1.5T MRI scanning at baseline, and were naturalistically followed-up over 1year. T1-weighted images were used to train a high-dimensional multivariate classifier, and to generate both spatial maps of the discriminative morphological patterns between groups and ROC curves. The spatial map discriminating first-episode schizophrenia patients from healthy controls revealed a complex pattern of regional volumetric abnormalities in the former group, affecting fronto-temporal-occipital gray and white matter regions bilaterally, including the inferior fronto-occipital fasciculus, as well as the third and lateral ventricles. However, an overall modest DA (73.4%) was observed for the individual discrimination between first-episode schizophrenia patients and controls, and the classifier failed to predict 1-year prognosis (remitting versus non-remitting course) of first-episode schizophrenia (DA=58.3%). In conclusion, using a “real world” sample recruited with epidemiological methods, the application of a neuroanatomical pattern classifier afforded only modest DA to classify first-episode schizophrenia subjects and next-door healthy controls, and poor discriminative power to predict the 1-year prognosis of first-episode schizophrenia

Incidence and risk factors for Preeclampsia in a cohort of healthy nulliparous pregnant women: a nested case-control study

The objective of this study is to determine the incidence, socio-demographic and clinical risk factors for preeclampsia and associated maternal and perinatal adverse outcomes. This is a nested case-control derived from the multicentre cohort study Preterm SAMBA, in five different centres in Brazil, with nulliparous healthy pregnant women. Clinical data were prospectively collected, and risk factors were assessed comparatively between PE cases and controls using risk ratio (RR) (95% CI) plus multivariate analysis. Complete data were available for 1,165 participants. The incidence of preeclampsia was 7.5%. Body mass index determined at the first medical visit and diastolic blood pressure over 75 mmHg at 20 weeks of gestation were independently associated with the occurrence of preeclampsia. Women with preeclampsia sustained a higher incidence of adverse maternal outcomes, including C-section (3.5 fold), preterm birth below 34 weeks of gestation (3.9 fold) and hospital stay longer than 5 days (5.8 fold) than controls. They also had worse perinatal outcomes, including lower birthweight (a mean 379 g lower), small for gestational age babies (RR 2.45 [1.52-3.95]), 5-minute Apgar score less than 7 (RR 2.11 [1.03-4.29]), NICU admission (RR 3.34 [1.61-6.9]) and Neonatal Near Miss (3.65 [1.78-7.49]). Weight gain rate per week, obesity and diastolic blood pressure equal to or higher than 75 mmHg at 20 weeks of gestation were shown to be associated with preeclampsia. Preeclampsia also led to a higher number of C-sections and prolonged hospital admission, in addition to worse neonatal outcomes9CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ401636/2013-5Bill and Melinda Gates FoundationGates Foundation [OPP1107597]; CNPqNational Council for Scientific and Technological Development (CNPq) [401636/2013-5

Back to Basics: Pitting Edema and the Optimization of Hypertension Treatment in Incident Peritoneal Dialysis Patients (BRAZPD)

Systemic arterial hypertension is an important risk factor for cardiovascular disease that is frequently observed in populations with declining renal function. Initiation of renal replacement therapy at least partially decreases signs of fluid overload; however, high blood pressure levels persist in the majority of patients after dialysis initiation. Hypervolemia due to water retention predisposes peritoneal dialysis (PD) patients to hypertension and can clinically manifest in several forms, including peripheral edema. The approaches to detect edema, which include methods such as bioimpedance, inferior vena cava diameter and biomarkers, are not always available to physicians worldwide. For clinical examinations, the presence of pitting located in the lower extremities and/or over the sacrum to diagnose the presence of peripheral edema in their patients are frequently utulized. We evaluated the impact of edema on the control of blood pressure of incident PD patients during the first year of dialysis treatment. Patients were recruited from 114 Brazilian dialysis centers that were participating in the BRAZPD study for a total of 1089 incident patients. Peripheral edema was diagnosed by the presence of pitting after finger pressure was applied to the edematous area. Patients were divided into 2 groups: those with and without edema according to the monthly medical evaluation. Blood arterial pressure, body mass index, the number of antihypertensive drugs and comorbidities were analyzed. We observed an initial BP reduction in the first five months and a stabilization of blood pressure levels from five to twelve months. The edematous group exhibited higher blood pressure levels than the group without edema during the follow-up. The results strongly indicate that the presence of a simple and easily detectable clinical sign of peripheral edema is a very relevant tool that could be used to re-evaluate not only the patient's clinical hypertensive status but also the PD prescription and patient compliance

The activity of laurel essential oil (crude and fractions) in the control of adult bovine ticks and larvae

Ectoparasites cause direct and indirect losses to farmers, affecting the production of meat and milk and increasing the production costs due to the acquisition of acaricides to maintain cattle health. The financial losses caused by Rhipicephalus (Boophilus) microplus, which is the main livestock ectoparasite in Brazil, reach approximately US$ 3 billion annually. The aim of this study was to evaluate the acaricidal effect of the crude essential oil (EO) and EO’s fractions (FR) obtained from Laurus nobilis L. (Lauraceae) leaves on Rhipicephalus (Boophilus) microplus. Eight fractions were obtained, wherein five major compounds were identified (sabinene, α-terpinyl acetate, 1,8-cineole, linalool, and α-terpineol). The acaricidal activity of these FR was tested by the larval packet test. The EO was tested by the adult immersion test, and, at concentrations of 200, 100 and 50 µL/mL, the oil caused mortality of engorged females, egg mass reduction, and hatching inhibition. The fractions with α-terpineol and sabinene, as the major compounds, were the most active larvicides (LC50=0.13 µL/mL, LC99=0.51 µL/mL; and LC50=0.20 µL/mL, LC99=0.56 µL/mL, respectively). This assessment also indicated that fractionation was important since most of the fractions obtained were more active than the EO. Furthermore, this is the first report of laurel EO and its fractions employed in the control of cattle ticks. Thereby, new prospects for the use of this essential oil or its chromatographic fractions in products applied for cattle tick control can be opened up. However, studies in other stages of development of cattle ticks for the active fractions, and studies under field conditions, the effect on non-target organisms and residual effect on the environment are still needed to evaluate the acaricidal activity of EO and its active fractions

Gender Differences in COVID-19 Among Liver Transplant Recipients: Results from a Multicenter Brazilian Cohort

Introduction: Existing literature presents varying perspectives on the impact of COVID-19 on liver transplant recipients.However, no research has specifically investigated the role of gender differences in the manifestation of COVID-19 among liver transplant recipients. This study aims to examine the effects of COVID-19 on liver transplant recipients, with a focus on gender differences in disease presentation and progression. Methods: Conducted as a multicenter historical cohort study, this research collected patient records through an online questionnaire. Assessing COVID-related mortality was the main objective. Additionally, demographic, clinical, and laboratory data pertaining to disease presentation and progression werecollected. Results: The study included a total of 283 patients, of whom 76 were female and 206 were male. The median follow-up period for males was 99 days (IQR 38-283), while for females, it was 126 days (IQR 44-291). A higher prevalence of cardiovascular disease was observed in males (p=0.002). Females frequently experienced a loss of smell (p=0.021), whereas males commonly exhibited fever (p=0.031). Levels of ALT and gamma-glutamyl transferase were significantly elevated in males (p=0.008 and 0.004, respectively). Although there was a trend towards increased mortality in males, it did not reach statistical significance. Conclusion: This study is the first attempt to investigate gender differences in COVID-19 among liver transplant recipients. Our findings highlight the need for a comprehensive and personalised approach to treating this patient population and underscore the importance of further elucidating the disease presentation in these individuals