Myositis ossificans of the masseter muscle: a rare location. Report of a case and review of literature

Background: Myositis Ossificans is a rare heterotopic bone formation within a muscle being the masticatory muscles exceptionally involved. In most cases there is a previous trauma, bearing in mind that there may be many other etiologies. CT scan and panoramic radiographs along with histological findings are essential diagnostic aids. Case Desciption: we report a rare case of MO of masseter muscle in 49 years-old woman after repetitive wisdom tooth infection with the discussion of clinical, radiological and histological features. Clinical Implications: MO is a rare disease of masticatory muscles being the masseter the most frequently affected. Wide surgical excision with free margins is the treatment of choice although close postoperative monitoring it’s essential to avoid relapses

Integrated analytical approach in veal calvesadministered the anabolic androgenic steroidsboldenone and boldione: urine and plasma kineticprofile and changes in plasma protein expression

Surveillance of illegal use of steroids hormones in cattle breeding is a key issue to preserve human health. To this purpose, an integrated approach has been developed for the analysis of plasma and urine from calves treated orally with a single dose of a combination of the androgenic steroids boldenone and boldione. A quantitative estimation of steroid hormones was obtained by LC-APCI-QMS/ MS analysis of plasma and urine samples obtained at various times up to 36 and 24 h after treatment, respectively. These experiments demonstrated that boldione was never found, while boldenone a- and b-epimers were detected in plasma and urine only within 2 and 24 h after drug administration, respectively. Parallel proteomic analysis of plasma samples was obtained by combined 2-DE,MALDI-TOF-MS and mLC-ESI-IT-MS/MS procedures. A specific protein, poorly represented in normal plasmasamples collected before treatment,was found upregulated even 36 h after hormone treatment.Extensivemassmapping experiments proved this component as an N-terminal truncated form of apolipoprotein A1 (ApoA1), a protein involved in cholesterol transport. The expression profile of ApoA1 analysed byWestern blot analysis confirmed a significant and time dependent increase of thisApoA1 fragment. Then, provided that further experiments performed with a growth-promoting schedule will confirm these preliminary findings, truncated ApoA1 may be proposed as a candidate biomarker for steroid boldenone and possibly other anabolic androgens misuse in cattle veal calves, when no traces of hormones are detectable in plasma or urine

Proof of nicotine transfer to rat pups through maternal breast feeding to evaluate the neurobehavioral consequences of nicotine exposure

This study investigates the passage of nicotine from lactating dams to their offspring through breast milk in the frame of a research focused to ascertain toxicological and neuro-behavioural effects on pups as consequence of either unavoidable (“passive”) or voluntary (“active”) maternal nicotine exposure. To this aim, plasmatic concentrations of nicotine and cotinine were determined by LC-MS/MS in Wistar rat pups whose mothers were orally administered with nicotine during lactation. Mothers were divided into voluntary consumption group, unavoidable consumption group, and controls. The limits of detection and quantification of the LC-MS/MS method were 0.20 and 0.65 ng/mL, respectively. Within-laboratory reproducibility (CV%) was <10.0%, with recovery of 86.2-118.8%. Results showed the presence of nicotine in 67% of samples from voluntary consumption group (1.30±0.31 ng/mL) and in 60% of samples from “passive” consumption group (1.19±0.62 ng/mL); cotinine was found in all the samples from voluntary (1.92±0.77 ng/mL) and  “passive” consumption groups (1.43±0.30 ng/mL). Data provide an evidence-based support to maternal/offspring nicotine transfer as function of different ways of oral exposure

COMPARAÇÃO DE TRÊS MÉTODOS DE ANÁLISE QUÍMICA UTILIZANDO GRAMA BERMUDA (Cynodon dactylon) COMO MATRIZ ANALÍTICA

Este trabalho foi realizado com objetivo de conhecer a eficiência dos procedimentos de análise foliar e de raiz, através da comparação de três metodologias para de análise química foliar e de raiz para a quantificação de potássio, sódio e fósforo. Os métodos avaliados foram a digestão via seca em cadinhos de porcelana, digestão nitro-perclórica e extração via úmida com ácido clorídrico. As amostras foram coletadas no setor de agronomia no Centro Universitário da Fundação Educacional de Barretos, e analisadas no laboratório de solos da mesma entidade. Analisaram-se amostras da grama-bermuda (Cynodon dactylon), enriquecida com lodo de esgoto. Na avaliação do método digestão via seca em cadinhos de porcelana obteve-se melhor sensibilidade para leitura do potássio na raiz da planta; na metodologia via úmida nitro-perclórica obteve-se melhor resultado para fósforo tanto na parte aérea quanto na raiz, também para o potássio na parte aérea e para o sódio na raiz; a extração via úmida em ácido clorídrico obteve-se um bom resultado do sódio na parte aérea

Synthesis of hydroxystilbenes and their derivatives via Heck reaction

A group of differently substituted acetoxy- and methoxystilbenes were synthesised via Heck reaction. A comparison between the use - as coupling reagents - of iodobenzene derivatives and diazonium salts was performed. A successful microwave-assisted demethylation by pyridine hydrochloride of some methoxystilbenes was carried out

Liquid chromatography with tandem mass spectrometry method for the determination of nicotine and minor tobacco alkaloids in electronic cigarette refill liquids and second-hand generated aerosol

A liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of nicotine and seven minor tobacco alkaloids in both refill liquids for electronic cigarettes and their generated aerosol was developed and validated. The limit of detection and limit of quantification values were 0.3–20.0 and 1.0–31.8 ng/mL, respectively. Within-laboratory reproducibility was 8.2–14.2% at limit of quantification values and 4.8–12.7% at other concentration levels. Interday recovery was 75.8–116.4%. The method was applied to evaluate the compliance of commercial liquids (n = 95) with their labels and to assess levels of minor alkaloids. Levels of nicotine and its corresponding compounds were also evaluated in generated aerosol. About 47% of samples showed differences above ±10 % of the stated nicotine concentration. About 78% of the “zero nicotine” liquids showed traces in the range of 1.3 ± 0.1–254.0 ± 14.6 μg/mL. Nicotine-N′-oxides, myosmine, and anatabine were the most common minor alkaloids in liquids containing nicotine. Nicotine and N′-oxides were detected in all air samples when aerosol was generated from liquids containing nicotine. Nicotine average emissions from electronic cigarette (2.7 ± 0.9 μg/m3) were significantly lower (p < 0.01, t-test) with respect to conventional cigarette (30.2 ± 1.5 μg/m3)

Magnetic Resonance Imaging of Primary Adult Brain Tumors: State of the Art and Future Perspectives

MRI is undoubtedly the cornerstone of brain tumor imaging, playing a key role in all phases of patient management, starting from diagnosis, through therapy planning, to treatment response and/or recurrence assessment. Currently, neuroimaging can describe morphologic and non-morphologic (functional, hemodynamic, metabolic, cellular, microstructural, and sometimes even genetic) characteristics of brain tumors, greatly contributing to diagnosis and follow-up. Knowing the technical aspects, strength and limits of each MR technique is crucial to correctly interpret MR brain studies and to address clinicians to the best treatment strategy. This article aimed to provide an overview of neuroimaging in the assessment of adult primary brain tumors. We started from the basilar role of conventional/morphological MR sequences, then analyzed, one by one, the non-morphological techniques, and finally highlighted future perspectives, such as radiomics and artificial intelligence

Magnetic Resonance Imaging of Primary Adult Brain Tumors: State of the Art and Future Perspectives

MRI is undoubtedly the cornerstone of brain tumor imaging, playing a key role in all phases of patient management, starting from diagnosis, through therapy planning, to treatment response and/or recurrence assessment. Currently, neuroimaging can describe morphologic and non-morphologic (functional, hemodynamic, metabolic, cellular, microstructural, and sometimes even genetic) characteristics of brain tumors, greatly contributing to diagnosis and follow-up. Knowing the technical aspects, strength and limits of each MR technique is crucial to correctly interpret MR brain studies and to address clinicians to the best treatment strategy. This article aimed to provide an overview of neuroimaging in the assessment of adult primary brain tumors. We started from the basilar role of conventional/morphological MR sequences, then analyzed, one by one the, non-morphological techniques, and finally highlighted future perspectives, such as radiomics and artificial intelligence

Magnetic Resonance Imaging of Primary Adult Brain Tumors: State of the Art and Future Perspectives

MRI is undoubtedly the cornerstone of brain tumor imaging, playing a key role in all phases of patient management, starting from diagnosis, through therapy planning, to treatment response and/or recurrence assessment. Currently, neuroimaging can describe morphologic and non-morphologic (functional, hemodynamic, metabolic, cellular, microstructural, and sometimes even genetic) characteristics of brain tumors, greatly contributing to diagnosis and follow-up. Knowing the technical aspects, strength and limits of each MR technique is crucial to correctly interpret MR brain studies and to address clinicians to the best treatment strategy. This article aimed to provide an overview of neuroimaging in the assessment of adult primary brain tumors. We started from the basilar role of conventional/morphological MR sequences, then analyzed, one by one, the non-morphological techniques, and finally highlighted future perspectives, such as radiomics and artificial intelligence