Parameterized thermal macromodeling for fast and effective design of electronic components and systems

We present a parameterized macromodeling approach to perform fast and effective dynamic thermal simulations of electronic components and systems where key design parameters vary. A decomposition of the frequency-domain data samples of the thermal impedance matrix is proposed to improve the accuracy of the model and reduce the number of the computationally costly thermal simulations needed to build the macromodel. The methodology is successfully applied to analyze the impact of layout variations on the dynamic thermal behavior of a state-of-the-art 8-finger AlGaN/GaN HEMT grown on a SiC substrate

Quinoa (Chenopodium quinoa Willd.) flour as novel and safe ingredient in bread formulation.

Aims of this study were to investigate the protein fraction of quinoa flour and to evaluate its in-vitro digestibility for bread formulation. The chemical composition of quinoa protein isolate and flour were investigated. Quinoa flour showed an excellent nutritional profile, including a high protein (about 14%), lipid (about 7%) and ash (about 2%) content. Proteomic and R5 ELISA analyses showed absence of gluten, confirming quinoa as a naturally gluten-free crop. The microstructure of flour and protein isolate, dough and quinoa bakery product were observed through Scanning Electron Microscopy (SEM). Furthermore, we studied the protein fraction of quinoa flour and protein isolate and to evaluate their in-vitro digestibility for a functional bread development using a static in vitro model of protein gastrointestinal digestion Romano et al. (2017). MS/MS analysis of gastrointestinal digests had a high degree of digestibility and survival of only few resistant peptides, none of which recognized by western blotting with sera of individuals allergic to cereals nor by in silico screening on allergenic sequence databases. Bakery product exclusively based on quinoa flour was prepared with valid nutritional properties. Results indicated that quinoa flour had a high degree of digestibility, supporting its excellent nutritional value and the use of quinoa as ingredient in substitutive dough formulations

Advancements towards restoration of the endangered limpet Patella ferruginea Gmelin, 1791 through controlled reproduction

The ferruginous limpet is one of the most threatened invertebrate species in the Mediterranean Sea. Its controlled reproduction has been considered one of the potentially most effective strategies for the production of juveniles for population restocking or for recolonization of areas where the species was brought to extinction by human pressure but is now under protection (e.g., MPAs). This approach has been pursued for at least two decades but with partial results: at most, some juveniles have been obtained and have reached adult stage, yet not starting from spawning induction but through sacrifice of female specimens (lethal approach), additionally resulting in low genetic diversity of the juveniles produced. Herein, we describe, for the first time, positive results of the spawning induction in P. ferruginea. The fertilizations made after these experiences allow describing the larval development of the species from its early stages to its metamorphosis. The fertilization rate in these spawning events was much higher (>97%) than in previous studies that were based on the extraction of oocytes by dissection. The rate of non-anomalous larvae after 15 hours was not negligible but variable, depending perhaps on the quality of the sperm available at each fertilization. The timing of larval development of the species and its variability, particularly regarding metamorphosis, are described. Settlement ability is reached at 3\u20134 days after fertilization, depending on water temperature, while a large variability is observed in metamorphosis, occurring between 7 and 32 days (probably up to 40 days), although inducing factors are still unknown

Ampiezza e tassi dei movimenti verticali a Capo Vaticano (Calabria occidentale,Italia) negli ultimi 20 mila anni determinati sulla base di cunei progradanti epiattaforme di abrasione.

Ampiezza e tassi dei movimenti tettonici verticali sono stati quantificati nel settore offshore di Capo Vaticano (Calabria occidentale), nell’intervallo Pleistocene superiore – Olocene, sulla base delle profondità del ciglio dei cunei progradanti infralitorali e delle piattaforme di abrasione formatesi durante l’ultimo massimo glaciale (LGM). I cunei progradanti sono stati riconosciuti in profili sismici a riflessione ad alta risoluzione Sparker. I dati sismici sono stati acquisiti lungo la piattaforma e la scarpata continentale superiore, durante le crociere oceanografiche Marisk 2010 e 2012 organizzate dall’IAMC del CNR di Napoli, il DISTEM dell’Università di Palermo e il Dip. di Scienze della Terra dell’Università di Napoli. La deformazione tettonica verticale del promontorio di Capo Vaticano e del suo prolungamento offshore è caratterizzata da una marcata asimmetria, con profondità dei cigli dei cunei infralitorali che si approfondisce progressivamente procedendo verso NE. La rimozione della componente non tettonica dei movimenti verticali, ottenuta utilizzando dati sulle variazioni glacio-eustatiche del tardo Quaternario [Lambeck et al., 2011], indica nell’area in esame ~11 (± 5) m di sollevamento e di ~25 (± 5) m di subsidenza, nell’intervallo post-LGM, muovendoci da sud-ovest verso nord-est, su una distanza di ~22 km. Il valore medio del tasso di sollevamento e di subsidenza (considerando la componente sia regionale sia locale) per gli ultimi 20.350 (± 1,35) anni sono pari a 0,52 (± 0,28) mm/anno e di 1,23 (± 0,32) mm/anno, rispettivamente. I valori dell’ampiezza e il pattern dei movimenti verticali ottenuti attraverso l’analisi dei cunei progradanti infralitorali sono comparabili, sebbene a tassi parzialmente differenti, sia con quelli determinati attraverso marker geomorfologici tardo Olocenici [Spampinato et al., 2012] che con i tassi di sollevamento a lungo termine calcolati sulla base delle posizioni dei terrazzi marini formatisi a 80 a 215 mila anni [Cucci & Tertulliani, 2010]. L’integrazione dei nuovi dati con quelli disponibili in letteratura indica che il basculamento del promontorio di Capo Vaticano è episodico ed è avvenuto principalmente tra 215 e 125 mila anni e nel post-LGM

Article microgravity induces transient emt in human keratinocytes by early down-regulation of e-cadherin and cell-adhesion remodeling

Changes in cell–matrix and cell-to-cell adhesion patterns are dramatically fostered by the microgravity exposure of living cells. The modification of adhesion properties could promote the emergence of a migrating and invasive phenotype. We previously demonstrated that short exposure to the simulated microgravity of human keratinocytes (HaCaT) promotes an early epithelial– mesenchymal transition (EMT). Herein, we developed this investigation to verify if the cells maintain the acquired invasive phenotype after an extended period of weightlessness exposure. We also evaluated cells’ capability in recovering epithelial characteristics when seeded again into a normal gravitational field after short microgravity exposure. We evaluated the ultra-structural junctional features of HaCaT cells by Transmission Electron Microscopy and the distribution pattern of vinculin and E-cadherin by confocal microscopy, observing a rearrangement in cell–cell and cell–matrix interactions. These results are mirrored by data provided by migration and invasion biological assay. Overall, our studies demonstrate that after extended periods of microgravity, HaCaT cells recover an epithelial phenotype by re-establishing E-cadherin-based junctions and cytoskeleton remodeling, both being instrumental in promoting a mesenchymal–epithelial transition (MET). Those findings suggest that cytoskeletal changes noticed during the first weightlessness period have a transitory character, given that they are later reversed and followed by adaptive modifications through which cells miss the acquired mesenchymal phenotype

The role of the anterolateral ligament in knee’s biomechanics: a case–control retrospective study

Purpose: The aim of this study was to assess the functional and clinical results of patients who underwent ACL reconstruction surgery and were divided into subpopulations related to ACL-associated lesions and focused on ALL-associated lesion. Methods: Our retrospective analysis included 62 patients who underwent standard ACL reconstruction surgery in our hospital from 2014 to 2016. The mean follow-up period was 21 months (range 11–35). We divided the sample into two subpopulations due to the presence or absence of ALL tear at the preoperative MRI. In 42 patients out of 62 (68%), ALL lesion was evident. We evaluated in both subpopulations the ACL failure rate, the functional outcomes rated with IKDC, KOOS, Lysholm scores and the clinical assessment of anteroposterior and rotatory instability with the Lachman test and pivot-shift test. Results: The overall re-injury rate in our cohort of patients was 4.8% with a smaller but not a significant difference between the two groups. A statistically significant difference was observed for the three functional scores, favoring the isolated ACL-lesion group (p < 0.05). Similarly, a better Lachman score was observed in the isolated ACL-lesion group, without statistical significance (p = 0.77); overall, the rate of positive test was lower in the isolated ACL-lesion group. We observed a significant difference of residual rotatory instability (positive pivot-shift test) in the two subpopulations (p = 0.036), and 9% of patients in the ACL + ALL lesion group showed residual jerk or subluxation. Conclusion: The additional ALL reconstruction/repair surgery should always be considered in patients with evident ALL tear at the preoperative MRI

The chiral 1:2 adduct (S)S(S)C(-)589-ethyl 2-phenylbutyl sulphide-mercury (II) chloride:(-)589[(S)S(S)C-Et(2-PhBu)S.(HgCl2)2]. Stereoselective synthesis, asymmetric oxidation, crystal and molecular structure and circular dichroism spectra

Optically active (-)589ethyl (S)-2-phenylbutyl thioether, (-)(S)C-Et(PhBu)S (I), and its new diastereoisomeric mercury (II) chloride adduct, 1:2, (-)[(S)S(S)C-Et(PhBu)S.(HgCl2)2]2, (II) were stereoselectively synthesized; the absorbance (UV) and circular dichroism (CD) spectra were measured and the crystal and molecular structure of complex (II) was determined by single-crystal X-ray diffraction. Two different Hg centres are present whose coordination environments are built by two short bonds to chloride ligands in one case, and to one chloride and one sulphur in the other one. These originate digonal units. Electroneutrality is achieved by a further chlorine, which can be considered prevalently ionic and bonded to the two Hg centres, forming square bridging systems nearly perpendicular to the digonal molecules. The coordination polyhedra can be interpreted as 2 + 4 tetragonally-compressed octahedra with the four longer contacts lying in the equatorial plane. IR spectroscopic data are consistent with the presence of one bent and one linear Cl–Hg–Cl moiety. The absolute configurations at both stereogenic centres of the formed diastereoisomeric complex (II) are (S). The (S)S absolute configuration at the stereogenic sulphur atom bonded to the mercury(II) atom in complex (II) has been related with the negative Cotton effect assigned in its circular dichroism (CD) spectrum to a charge-transfer transition at ca. 230 nm. The stereoselective oxidation of (I) and (II) with hydrogen peroxide, induced by the stereogenic carbon atom (S)C of the enantiopure sulphide, gave (-)598ethyl (S)C-2-phenylbutyl(S)S-sulphoxide, (-)598[(S)S(S)C-Et(PhBu)SO], (III), having 18.1% de. Oxidations carried out in the presence of a 200 molar excess of mercury(II) chloride gave (-)598ethyl (S)C-2-phenylbutyl(R)S-sulphoxide, (-) 598[(R)S(S)C-Et(PhBu)SO], (IV) with 31% de, showing the cooperative influence of mercury(II) chloride on the selectivity of the oxidation reaction

Proteomic study of muscle sarcoplasmic proteins using AUT-PAGE/SDS-PAGE as two-dimensional gel electrophoresis

The conversion of muscle to meat in pig involves mainly proteolysis of myofibrillar proteins, which undergo notable changes since early stage of rigor mortis, even after 48 h post mortem. The tenderness of meat has been thoroughly investigated to understand the biochemical mechanisms, which influence texture and flavour development as well as the technological parameters and hence meat quality. Cytoplasmic proteolytic calcium dependent enzymes, named -and m-calpains, which act in the early stages of rigor mortis, significantly contribute to tenderization weakening myofibrils. These enzymes, however, act for fewdays because they are specifically inhibited by calpastatin and by pH lowering. However, when pH falls to about 5.0, proteolytic activity on muscle proteins is continued by longer acting lysosomal proteinase, cathepsins [3,7–9]. Post mortem proteolysis also causes relevant changes in sarcoplamic protein fraction, which represent the water soluble fraction (quantitatively about 30–35%) of meat total protein, and the involved proteins has already been identified by proteomic-based studies. Recent investigations have demonstrated that the most commonly found Lactobacillus species in dry fermented meats are able to hydrolyse myofibrillar and sarcoplasmic muscle proteins in vitro.The most abundant sarcoplasmic proteins, as mixture of basic polypeptides with a narrow spread range of molecular masses, represented an excellent model to test our analytical technique and to delineate its capabilities. In the present study, we compared 2D AUT-PAGE/SDSPAGE maps of water-soluble proteins extracted from fresh meat and from dry-cured ham, a non fermented product, from “Naples-type” salami, a microbiologically fermented product, and from “Coppa”, a typical semi-fermented product. Electrophoretically separated proteins have been identified by MALDI-ToF mass fingerprinting

Computerized surveillance of opioid-related adverse drug events in perioperative care: a cross-sectional study

<p>Abstract</p> <p>Background</p> <p>Given the complexity of surgical care, perioperative patients are at high risk of opioid-related adverse drug events. Existing methods of detection, such as trigger tools and manual chart review, are time-intensive which makes sustainability challenging. Using strategic rule design, computerized surveillance may be an efficient, pharmacist-driven model for event detection that leverages existing staff resources.</p> <p>Methods</p> <p>Computerized adverse drug event surveillance uses a logic-based rules engine to identify potential adverse drug events or evolving unsafe clinical conditions. We extended an inpatient rule (administration of naloxone) to detect opioid-related oversedation and respiratory depression to perioperative care at a large academic medical center. Our primary endpoint was the adverse drug event rate. For all patients with a naloxone alert, manual chart review was performed by a perioperative clinical pharmacist to assess patient harm. In patients with confirmed oversedation, other patient safety event databases were queried to determine if they could detect duplicate, prior, or subsequent opioid-related events.</p> <p>Results</p> <p>We identified 419 cases of perioperative naloxone administration. Of these, 101 were given postoperatively and 69 were confirmed as adverse drug events after chart review yielding a rate of 1.89 adverse drug events/1000 surgical encounters across both the inpatient and ambulatory settings. Our ability to detect inpatient opioid adverse drug events increased 22.7% by expanding surveillance into perioperative care. Analysis of historical surveillance data as well as a voluntary reporting database revealed that 11 of our perioperative patients had prior or subsequent harmful oversedation. Nine of these cases received intraoperative naloxone, and 2 had received naloxone in the post-anesthesia care unit. Pharmacist effort was approximately 3 hours per week to evaluate naloxone alerts and confirm adverse drug events.</p> <p>Conclusion</p> <p>A small investment of resources into a pharmacist-driven surveillance model gave great gains in organizational adverse drug event detection. The patients who experienced multiple events are particularly relevant to future studies seeking risk factors for opioid induced respiratory depression. Computerized surveillance is an efficient, impactful, and sustainable model for ongoing capture and analysis of these rare, but potentially serious events.</p