32 research outputs found

    Nueva cepa de TRICHODERMA SATURNISPORUM, composiciones y aplicaciones de la misma

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    Número de publicación: 2 411 807 Número de solicitud: 201132042 51 Int. CI.: C12N 1/14 (2006.01) C12P 1/02 (2006.01) C12R 1/885 (2006.01)Nueva cepa de Trichoderma saturnisporum, composiciones y aplicaciones de la misma. La presente invención se refiere a una nueva cepa de Trichoderma saturnisporum con número de depósito CECT nº 20781. Asimismo se refiere a una composición fitosanitaria que comprende dicha cepa y a su empleo para el control biológico de plagas, para potenciar el crecimiento y/o producción de cultivos hortícolas o plantas y para la descomposición de materia orgánica

    Cladobotryum mycophilum as Potential Biocontrol Agent

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    A study was conducted to explore the efficacy of potential biocontrol agent Cladobotryum mycophilum against different phytopathogenic fungi. The growth rates of 24 isolates of C. mycophilum were determined, and their antagonistic activity was analysed in vitro and in vivo against Botrytis cinerea, Fusarium oxysporum f. sp. radicis-lycopersici, Fusarium oxysporum f.sp. cucumerinum, Fusarium solani, Phytophthora parasitica, Phytophthora capsici, Pythium aphanidermatum and Mycosphaerella melonis. Most isolates grow rapidly, reaching the opposite end of the Petri dish within 72–96 h. Under dual-culture assays, C. mycophilum showed antagonistic activity in vitro against all phytopathogenic fungi tested, with mycelial growth inhibition ranging from 30 to 90% against all the different phytopathogens tested. Similarly, of all the selected isolates, CL60A, CL17A and CL18A significantly (p < 0.05) reduced the disease incidence and severity in the plant assays compared to the controls for the different pathosystems studied. Based on these results, we conclude that C. mycophilum can be considered as a potential biological control agent in agriculture. This is the first study of Cladobotryum mycophilum as a biological control agent for different diseases caused by highly relevant phytopathogens in horticultur

    Plant Growth Promotion and Biocontrol of Pythium ultimum by Saline Tolerant Trichoderma Isolates under Salinity Stress

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    This present study evaluates three isolates of Trichoderma as plant growth promoting or biological control agents: Trichoderma aggressivum f. sp. europaeum, Trichoderma saturnisporum, and the marine isolate obtained from Posidonia oceanica, Trichoderma longibrachiatum. The purpose is to contribute to an overall reduction in pesticide residues in the fruit and the environment and to a decrease in chemical fertilizers, the excess of which aggravates one of the most serious abiotic stresses, salinity. The tolerance of the different isolates to increasing concentrations of sodium chloride was evaluated in vitro, as well as their antagonistic capacity against Pythium ultimum. The plant growth promoting capacity and effects of Trichoderma strains on the severity of P. ultimum on melon seedlings under saline conditions were also analysed. The results reveal that the three isolates of Trichoderma, regardless of their origin, alleviate the stress produced by salinity, resulting in larger plants with an air-dry weight percentage above 80% in saline stress conditions for T. longibrachiatum, or an increase in root-dry weight close to 50% when T. aggressivum f. sp. europaeum was applied. Likewise, the three isolates showed antagonistic activity against P. ultimum, reducing the incidence of the disease, with the highest response found for T. longibrachiatum. Biological control of P. ultimum by T. aggressivum f. sp. europaeum and T. saturnisporum is reported for the first time, reducing disease severity by 62.96% and 51.85%, respectively. This is the first description of T. aggressivum f. sp. europaeum as a biological control agent and growth promoter. The application of these isolates can be of enormous benefit to horticultural crops, in both seedbeds and greenhouses

    Screening and Evaluation of Essential Oils from Mediterranean Aromatic Plants against the Mushroom Cobweb Disease, Cladobotryum mycophilum

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    The main aim of this study was to evaluate the use of essential oils (EOs) as an alternative to synthetic fungicides used in the control of cobweb disease of button mushroom (Agaricus bisporus) caused by Cladobotryum mycophilum. The EOs used were obtained by hydrodistillation from five Mediterranean aromatic species (Lavandula × intermedia, Salvia lavandulifolia, Satureja montana, Thymus mastichina, and Thymus vulgaris), analyzed by gas chromatography, and tested in vitro for their antifungal activity against C. mycophilum. In vitro bioassays showed that the EOs obtained from T. vulgaris and S. montana (ED50 = 35.5 and 42.8 mg L−1, respectively) were the most effective EOs for inhibiting the mycelial growth of C. mycophilum, and were also the most selective EOs between C. mycophilum and A. bisporus. The in vivo efficacy of T. vulgaris and S. montana EOs at two different concentrations (0.5 and 1%) were evaluated in two mushroom growing trials with C. mycophilum inoculation. The treatments involving T. vulgaris and S. montana EOs at the higher dose (1% concentration) were as effective as fungicide treatment. The effect of these EOs on mushroom productivity was tested in a mushroom cropping trial without inoculation. They had a strong fungitoxic effect at the first flush. However, a compensatory effect was observed by the end of the crop cycle and no differences were observed in biological efficiency between treatments. The main compounds found were carvacrol and p-cymene for S. montana, and p-cymene and thymol for T. vulgaris. These results suggest that T. vulgaris and S. montana EOs may be useful products to manage cobweb disease if used as part of an integrated pest management (IPM) program

    Influence of the application of Jasmonic Acid and Benzoic Acid on Watermelon fruit quality.

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    In watermelon crops in Southeastern Spain, important thermal differences appear during the first stages of plant development that can affect them. This work shows the effect of applying jasmonic acid and benzoic acid (JA+BA), inductors of systemic acquired resistance (SAR) and induced systemic resistance (ISR), respectively, on fruit quality parameters from a crop in a greenhouse in Southeastern Spain, where crops face a remarkable abiotic stress. We assessed two treatments of JA+BA, T1 (500+500 ppm), T2 (2000+2000 ppm) and a control test using an experimental design of randomized blocks with four replications. The results obtained for the parameters assessed (ºBrix, flesh firmness, rind thickness, polar and equatorial diameter) did not show statistically significant differences. The results showed that there was no metabolic cost in the plants when applying the assessed treatments of JA+BA

    Effect of the application of jasmonic acid and benzoic acid on grafted watermelons yield under greenhouse conditions in the southeast of Spain for mitigation of stress

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    One of the main problems of watermelon crops in Sou theast Spain is the thermal difference because of c limatic conditions that appear during the first stages of the crop. The objective of this work was to evaluate the effect of inducing the systemic acq uired resistance (SAR) and the induced systemic resistance (ISR) through the application of jasmonic ac id (JA) and benzoic acid (BA), respectively, to counter the abiotic stress. We assessed two treatments of JA and BA, T1 (500 mg·kg-1 + 500 mg·kg -1 ) and T 2 (2000 mg·kg -1 + 2000 mg·kg -1), as well as a control test using an experimental design of randomized blocks with four replications. The results obtained for kg·m -2, fruits/m², kg/plant and fruits/plant did not show statistically significant differences. However, we obtained statistically sig nificant differences in the average fruit weight co mpared with the control test in the two experiments carried out in 2009 and 2010. The results showed that there was no metabolic cost in the plants when applying the assessed treatments of JA and BA

    Nueva cepa de Paecilomyces variotii, composiciones y aplicaciones de la misma

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    Número de publicación: ES2684858 A1 (04.10.2018) También publicado como: ES2684858 B1 (09.07.2019) Número de Solicitud: Consulta de Expedientes OEPM (C.E.O.) P201700542 (31.03.2017)Nueva cepa de Paecilomyces variotii, composiciones y aplicaciones de la misma. La presente invención se refiere a una nueva cepa de Paecilomyces variotii, con número de depósito CECT nº 20957 y que se ha denominado Pae10773. Dadas las propiedades de esta nueva cepa, en la presente invención se contempla su uso para potenciar el crecimiento de plantas, tal como cultivos hortícolas. Asimismo, se refiere a una composición que comprende dicha cepa, con capacidad de promover el desarrollo de plantas en suelos infestados por patógenos o condiciones de estrés. Por ello, se describe su uso como biofertilizante para plantas.Universidad de Almerí

    Parameter Selection for the Evaluation of Compost Quality

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    This work studies variables measured from the first phase of composting through the acquisition of the final product, with the goal of identifying those that are more strongly related to quality and are most useful for developing an index. The necessity to establish quality control procedures thus exists for the classification of raw materials in the same way as for the finished products. To accomplish this, three mixtures were prepared, with the goal of achieving a C/N ratio of 30 and a moisture content of 60%. The primary component of each mixture was: fruit processing waste (C1), sewage sludge from the food industry (C2), and the manufacturing waste of fried foods (C3). Temperatures were measured over 107 days, with the corresponding data fit to a logistical model where T °C ~ α / ((1 + exp (− (Time − β) / − γ))) + δ, with interaction compost * time being statistically significant (p Bd and <TPS, total pore space) and higher content of Zn and Cu. Composting carried out with appropriate mixtures can offer high-quality products for use as fertiliser, in soil restoration, and as an alternative substrate to peat and virgin mountain soil

    Nueva cepa de Trichoderma aggressivum fsp europaeum, composiciones y aplicaciones de la misma

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    Número de publicación: ES2706099 A1 (27.03.2019) También publicado como: ES2706099 B2 (03.02.2020) Número de Solicitud: Consulta de Expedientes OEPM (C.E.O.) P201731151 (27.09.2017)Nueva Cepa de Trichoderma aggressivum fsp europaeum, composiciones y aplicaciones de la misma. La presente invención se refiere a una nueva cepa de Trichoderma aggressivum fsp europaeum, con número de depósito CECT nº 20974 y que se ha denominado Tae52481. Dadas las propiedades de esta nueva cepa, en la presente invención se contempla su uso para potenciar el crecimiento de plantas, tales como cultivos hortícolas u ornamentales. Asimismo, se refiere a una composición que comprende dicha cepa, con capacidad de promover el desarrollo de plantas en suelos salinos, con presencia de patógenos o condiciones de estrés. Por ello, se describe su uso como biofertilizante para plantas.Universidad de Almerí

    Almeria’s Green Pest Management Revolution: An Opportunity That Arose from a Food Safety Alert

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    Almería, a province of Spain, is the leader in horticultural production on a Spanish and European scale. The specific conditions of greenhouse cultivation favor plant development, but also the proliferation of pests. This high incidence of pests was controlled in the past mainly by means of chemical phytosanitary treatments. The aim of the present work has been to analyze the tools facilitated by the Andalusian Government (Junta de Andalucía) to replace the usual pest management process, taking advantage of the context of the food safety alert arising from the detection of isophenphos-methyl in peppers from the province of Almeria in December 2007. The results illustrate that, unlike many programs of public subsidies which involve long-term expenditure, the aid in question took advantage of the socioeconomic situation following the food safety alert. The program led to substantial economic savings and met its objectives swiftly, achieving excellent results in terms of removing most of the pesticides used in the “conventional production system”. In the 2006–2007 season, only 515 hectares in Almería used biological control organisms, four years later, it reached 20,081 hectares, and the average area during the last ten years was 24,953 hectares. This shows that Almeria’s green pest management revolution had been consolidated
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