A new design of a multifunctional abutment to morse taper implant connection: experimental mechanical analysis

The objective of this study was to evaluate a new design of multifunctional abutment for Morse taper implant connections, relative to the retentive stability after the application of cyclic loads in cemented and screwed crowns. Multifunctional abutments with two different angulations in the seating portion of the crown were tested, forming 2 groups (n = 30 samples per group): Group Abut11, where Smart abutments with an angle of 11.42° were used; Group Abut5, where Ideale abutments with an angle of 5° were used. Fifteen samples from each group received cemented crowns (CC) and another fifteen screwed crowns (SC). All crown samples were subjected to the mechanical cycling test at 360,000 cycles at a frequency of 4 Hz and 150 N of the load. The samples with CC were subjected to the tensile test to remove the crowns, while in the samples with SC, the detorque value of the fastening crown screws was measured. The mean tensile strength value of CC in the Abut11 group was 131.9 ± 13.5 N and, in the Abut5 group was 230.9 ± 11.3 N; while the detorque mean value in samples with SC 5.8 ± 1.8 N for the Abut11 group and, 7.6 ± 1.1 N for Abut5 group. Significant statistical differences were found between the two groups in both situations tested (p < 0.05). The multifunctional abutments, presenting a lesser angulation of the crown-seating portion, showed higher values of retention of the CC and a lesser screw loosening of torque of the fixing screws in the SC after the application of cyclic loads when compared to the abutments with more angulation in the crown-seating portion.info:eu-repo/semantics/publishedVersio

#102 Comparação da resistência á tração de diferentes membranas de fibrina rica em plaquetas

Objetivos: Este estudo teve por objectivo fazer comparação directa da resistência à tração entre membranas produzidas com diferentes protocolos de centrifugação, Leucocyte?Platelet Rich Fibrin (L? PRF) versus advanced? Platelet Rich Fibrin (a?PRF). Materiais e métodos: Após a colheita de sangue de uma pessoa saudável e sem histórico de toma de anticoagulantes ou outro medicamento, sob controlo alimentar, procedeu? se à confeção de membranas segundo os protocolos de L? PRF e a? PRF previamente descritos na literatura. De seguida, as membranas, n=26 (13 para cada protocolo), foram submetidas a um teste mecânico de tração, para os quais foram obtidos valores de tração máxima e de tração média. A análise estatística dos dados foi feita com o teste t?Student não pareado. Resultados: Relativamente à tração média, o protocolo a? PRF e L?PRF, respetivamente, foram de 0.0288 N.mm? 2 e 0.0192 N.mm? 2 (pinfo:eu-repo/semantics/publishedVersio

Sinus lift associated with leucocyte‐platelet‐rich fibrin (second generation) for bone gain: a systematic review

The purpose of this systematic review was to analyze sinus lifting procedures and to compare the efficiency of this treatment associated with the second generation of platelet‐rich fibrin related to its effects on bone gain and to clarify the regenerative efficacy in sinus lift procedure, whether alone or as a coadjutant to other bone graft materials. The PICOT question was, “In clinical studies with patients needing a maxillary sinus lift (P), does the use of PRF either alone (I) or in conjunction with other biomaterials (C) improve the clinical outcome associated with bone gain and density (O), with at least three months of follow‐up (T)?” An electronic search was conducted in the MEDLINE (PubMed), Science Direct, and Scopus databases through a search strategy. A total of 443 articles were obtained from the electronic database search. Sixteen articles met all criteria and were included in this review. Within the limitation of this study and interpreting the results carefully, it was suggested that a higher risk for implant failure after a sinus elevation might be seen in patients with residual bone ≤4 mm, and PRF application was effective, suggesting reducing the time needed for new bone formation.info:eu-repo/semantics/publishedVersio

Periodontal regeneration after third molar extraction causing attachment loss in distal and furcation sites of the second molar: a case report with 12 months follow-up

Introduction: The periodontal support apparatus is extremely important due to guarantee insertion to the tooth. It helps with the maintenance of the structural resistance and reconstructive property, and its loss may contribute to root exposure and the possibility to obtain an augmentation of the sensitivity. Thereby, this paper aimed to describe a case report, using regenerative techniques in a bone loss involving distal and furcation regions of the mandibular second molar, which was associated with the extraction of the adjacent impacted third molar without bone interposing. Case presentation: The patient was referred to a private dental clinic reporting an unpleasant odor while using dental floss in the distal region of the mandibular left second molar, during the three years. In addition, reported a history of extraction of the wisdom tooth six years ago. Periapical examination and periodontal index were performed and an extensive radiolucent image involving distal and furcation regions, with a 12 mm probing depth involving the distal root at tooth (2nd molar). No other region showed probing depth greater than 3mm. Thus, after rigorous scaling and root planing, periodontal guided tissue regeneration was performed as an attempt to fill the bone defect. Conclusion: The regenerative process is a predictable way to treat vital mandibular second molar after extraction of the adjacent third molar. Clinicians must give special attention to extraction involving the mandibular third molars, mainly when there is no bone interposed between the second and third molars.info:eu-repo/semantics/publishedVersio

Blood and salivary inflammatory biomarkers profile in patients with chronic kidney disease and periodontal disease: a systematic review

Introduction: Periodontitis is the most prevalent inflammatory disease worldwide. Its inflammatory levels spread systemically, which can be associated with chronic kidney disease. Biomarkers have the potential to diagnose and correlate periodontitis and chronic kidney disease, helping to monitor systemic inflammation. Thereby, this study aimed to analyze the association between chronic kidney disease and periodontitis by conducting a biomarker analysis on blood and saliva. Material and methods: An electronic search through PubMed/MEDLINE, EMBASE, and Web of Science databases was conducted to identify clinical studies published in the last ten years, with no language restrictions. Twelve articles met all the inclusion criteria, two randomized controlled trials, one cohort study, and nine observational studies. Results: The studies included a total of 117 patients for saliva biomarkers, with a mean age of approximately 57 years old, and 56.68% of the subjects were female. After analyzing all the included studies, it was possible to verify the following biomarkers assessed: CRP, WBC, fibrinogen, IL-4 and -6, cardiac troponin T, NOx, ADMA, albumin, osteocalcin, cystatin C, PGLYRP1, cholesterol, HDL, LDL, triglycerides, and hemoglobin. Conclusion: A direct cause–effect association between periodontitis and CKD could not be established. However, it was possible to conclude that there was a correlating effect present, through the analyzed biomarkers.info:eu-repo/semantics/publishedVersio

Histological and immunohistochemical analysis of a nanobiomaterial in a maxillary sinus lift surgery: a case report

The objective of this study was to histologically and immunologically analyze the level of bone substitution and the presence of new blood vessels in a nanobiomaterial composed of hydroxyapatite and BTCP in a maxillary sinus lift surgery. A case of a maxillary sinus lift was investigated. The patient had a 1.0 mm bone remnant on the left side and 2 grams of nanobiomaterial (80% hydroxyapatite and 20% β-tricalcium phosphate) were grafted into the pneumatized sinus. After 6 months, during dental implant placement surgery, grafted bone samples were collected with 4 mm trephines. These samples were sent to the laboratory for analysis where they were stained with masson'strichrome and immuno stained with osteonectin and osteopontin. After 6 months of bone regeneration a result of 14 mm of bone gain was reached, the analyzes in masson's trichrome showed an excellent gain of newly formed bone, in addition to a very high percentage of blood vessels. In the immunostaining, a very large number of osteoblasts and osteoclasts was observed, signaling an excellent osteoconduction and osteoinduction of the studied nanobiomaterial. The maxillary sinus lift surgery with nanobiomaterial provided a very favorable bone height and thickness gain, as well as the high vascularity and cellularity, which enabled the patient's oral rehabilitation with osseointegrated dental implants and permanent ceramic prostheses.info:eu-repo/semantics/publishedVersio

#080 Alongamento coronário com o uso do laser díodo e sistema piezoelétrico: relato de caso

Introdução: O uso do laser de díodo de alta intensidade em cirurgias periodontais proporciona maior precisão do corte cirúrgico e permite pouca absorção de luz pelos tecidos duros quando se utilizam parâmetros adequados, não gerando assim qualquer dano térmico. Outras vantagens seriam a mais rápida coagulação tecidual, a redução do tempo cirúrgico e a diminuição do risco de infeções pós?operatórias. Outro equipamento com crescente uso é o piezoelétrico, o qual também está indicado em cirurgias orais, a proporcionar também osteotomias mais precisas, limpas e com menor trauma para os tecidos moles. Portanto, o objetivo deste relato foi mostrar a utilização de tecnologias em procedimento estético periodontal. Descrição do caso clínico: Paciente do sexo feminino, 25 anos, saudável, com tratamento ortodôntico prévio, com queixa principal de grande exposição gengival ao sorrir. Planeou?se um alongamento coronário com uso do laser de díodo, em região estética superior (1.4 – 2.4). Após uso do laser para corte gengival, foi feito retalho de espessura total para visualização do osso de sustentação e posterior osteotomia com piezoelétrico, a seguir mensurações e proporções estéticas. Posteriormente, retalho foi reposicionado e suturado. No pós?operatório de 7 dias e 14 dias, pode? se confirmar a excelente recuperação do tecido local e da paciente. Discussão e conclusões: Existem evidências de que a cirurgia em tecidos moles com laser a díodo, e a cirurgia de tecidos duros com aparelho piezoelétrico, proporcionam um bom prognóstico e melhorando assim o pós? cirúrgico do paciente. O laser de díodo permite ter um campo cirúrgico limpo, sem hemorragia, diminuindo o risco de inflamação e infeção pós? cirúrgica quando comparado a sistema tradicional de cirurgias. A osteotomia com piezoeléctrico permite um corte preciso e menos traumático, proporcionando um menor perfil inflamatório a nível ósseo. As vantagens para o paciente são: diminuição da dor e do edema. Enquanto as vantagens para o profissional são: maior sensibilidade tátil e uma melhor visibilidade do campo operatório. Também proporciona proteção dos tecidos moles e das estruturas nobres adjacentes, incluindo um maior controlo da assepsia. A utilização destas tecnologias em cirurgia periodontal mostrou maior exequibilidade e visibilidade, campo cirúrgico mais limpo e menor hemorragia e edema. Inclusive, estas técnicas permitiram reparo ósseo e gengival mais favorável.info:eu-repo/semantics/publishedVersio

The international platform of registered systematic review and meta-analysis protocols (INPLASY) at 3 years: an analysis of 4,658 registered protocols on inplasy.com, platform features, and website statistics

Background: INPLASY® is an international platform for registering systematic reviews and meta-analysis protocols that was launched in March 2020. INPLASY® provides an online database in which the protocols are maintained as permanent public records and can be accessed on its website (www.inplasy.com). Methods: We described the database features and registered information of all records published since the launch of the registry on March 31, 2023. Additionally, we analyzed the website statistics dataset to explore user experience and promote data transparency. Results: Four thousand six hundred fifty-eight records were registered in INPLASY®, and more than 94% of the protocols were published within 24 h. Most of the submissions were from China, followed by Portugal, Taiwan, Malaysia, and Brazil. The INPLASY® website received 386,395 page views from 64,568 visitors during the first three years. The accesses were obtained from 170 countries. Most of the accesses were from China, followed by the US, the UK, and Portugal. The review status “completed and published” was observed in 898 protocols, and these studies were published in 372 different scientific peer-reviewed journals. The features of INPLASY® include the following: (i) INPLASY® identifier, a unique protocol number; (ii) the digital object identifier (DOI) number, the URL of the protocol linked to a specific DOI; (iii) ORCID update, INPLASY® automatically updates authors' ORCID page, including their protocol; and (iv) search tools, the protocols are freely accessible on www.inplasy.com. Conclusions: INPLASY® has several practical and useful features that should be considered when planning the registration of a systematic review protocol. Furthermore, the sharp increase in the number of protocols registered in INPLASY® in the first three years and the database statistics demonstrate that INPLASY® has become an important source of systematic review protocols. Therefore, authors should access INPLASY® before planning a future review study to avoid unintended duplication of efforts and to obtain timely registration.publishedVersio

Tensile strength essay comparing three different platelet-rich fibrin membranes (L-PRF, A-PRF, and A-PRF plus): a mechanical and structural in vitro evaluation

Predictable outcomes intended by the application of PRF (platelet-rich fibrin) derivative membranes have created a lack of consideration for their consistency and functional integrity. This study aimed to compare the mechanical properties through tensile strength and analyze the structural organization among the membranes produced by L-PRF (leukocyte platelet-rich fibrin), A-PRF (advanced platelet-rich fibrin), and A-PRF+ (advanced platelet-rich fibrin plus) (original protocols) that varied in centrifugation speed and time. L-PRF (n = 12), A-PRF (n = 19), and A-PRF+ (n = 13) membranes were submitted to a traction test, evaluating the maximum and average traction. For maximum traction, 0.0020, 0.0022, and 0.0010 N·mm−2 were obtained for A-PRF, A-PRF+, and L-PRF, respectively; regarding the average resistance to traction, 0.0012, 0.0015, and 0.006 N·mm−2 were obtained, respectively (A-PRF+ > A-PRF > L-PRF). For all groups studied, significant results were found. In the surface morphology observations through SEM, the L-PRF matrix showed a highly compact surface with thick fibers present within interfibrous areas with the apparent destruction of red blood cells and leukocytes. The A-PRF protocol showed a dense matrix composed of thin and elongated fibers that seemed to follow a preferential and orientated direction in which the platelets were well-adhered. Porosity was also evident with a large diameter of the interfibrous spaces whereas A-PRF+ was the most porous platelet concentrate with the greatest fiber abundance and cell preservation. Thus, this study concluded that A-PRF+ produced membranes with significant and higher maximum traction results, indicating a better viscoelastic strength when stretched by two opposing forces.info:eu-repo/semantics/publishedVersio

Fibroblasts and osteoblasts behavior after contact with different titanium surfaces used as implant abutment: An in vitro experimental study

Background: The goal of this in vitro study was to compare three different surfaces: two types of implant surfaces commercially available ([a] smooth/machined and [b] acid-treated surface) versus (c) anodized surface. Discs were manufactured with commercially pure titanium (CP) grade IV, which were subsequently analyzed by scanning microscopy and fibroblastic and osteoblastic cell cultures. Methods: Ninety-nine discs (5 ×2 mm) were manufactured in titanium grade IV and received different surface treatments: (i) Mach group: machined; (ii) AA group: double acid etch; and (iii) AN group: anodizing treatment. Three discs from each group were analyzed by Scanning Electron Microscopy (SEM) to obtain surface topography images and qualitatively analyzed by EDS. Balb/c 3T3 fibroblasts and pre-osteoblastic cells (MC3T3-E1 lineage) were used to investigate each group’s biological response (n =10/cellular type). The data were compared statistically using the ANOVA one-way test, considered as a statistically significant difference p Mach >AN). There was similar behavior for cell adhesion for the test groups (Mach and AN), with greater adhesion of Balb/c 3T3 fibroblast