Functions of DNA damage machinery in the innate immune response to DNA virus infection.

DNA is potently immunostimulatory, and self-DNA is packaged in the nucleus or mitochondria allowing it to remain silent to cell-intrinsic sensors. However, damaged or mislocalised self-DNA is sensed by our innate immune systems, resulting in the production of type I interferons (IFNI), chemokines and inflammatory cytokines. During DNA virus infection the detection of viral DNA genomes by pattern recognition receptors (PRRs) is essential for the initiation of IFNI responses and host defence against these pathogens. It is intriguing that a number of molecular mechanisms have been found to be common to both of these DNA-induced stress responses and this has potentially important consequences for both sides of the host/pathogen arms race.This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.coviro.2015.08.001

American White Pelican (\u3ci\u3ePelecanus erythrorhynchos\u3c/i\u3e) Growth, Nutrition and Immunology

Limited information about nutrition exists on American White Pelicans (Pelecanus erythrorhynchos) from hatching to fledging. To detail immunity, metabolism and nutrition of juvenile American White Pelicans, during 22-23 July 2011, 103 samples of regurgitate matter were collected at five Chase Lake, North Dakota, USA, and three Bitter Lake, South Dakota, USA, sub-colonies. Regurgitate sample nutrient content was significantly different for organic matter (P = 0.012), crude protein (P = 0.001), neutral detergent fiber (P = 0.014), acid detergent fiber (P = 0.005) and energy (P = 0.034) between North (n = 5) and South (n = 3) Dakota American White Pelican colonies. Average concentrations of immunoglobulins Y (2.74 ± 1.85 ng/mL) and A (9.04 ± 9.41 ng/mL) demonstrated passive transfer of immunity in regurgitate. To enhance information on growth and morphology in hand-reared American White Pelicans (n = 8), a 9-week captive trial was also conducted raising chicks from hatching to fledging. Predictive models were created to describe chick growth for intake, body weight, culmen length and tarsus length. Data collected during this study enhances both American White Pelican general ecology and conservation with implications for both captive and wild bird management

A Scenario-based Parametric Analysis of the Army Personnel-to-assignment Matching Problem

This study aims to compare linear programming and stable marriage approaches to the personnel assignment problem under conditions of uncertainty. Robust solutions should exhibit reduced variability of solutions in the presence of one or more additional constraints or problem perturbations added to some baseline problems.Several variations of each approach are compared with respect to solution speed, solution quality as measured by officer-to-assignment preferences and solution robustness as measured by the number of assignment changes required after inducing a set of representative perturbations or constraints to an assignment instance. These side constraints represent the realistic assignment categorical priorities and limitations encountered by army assignment managers who solve this problem semiannually, and thus the synthetic instances considered herein emulate typical problem instances

Using 360-Degree Video for Immersive Learner Engagement

A 360-degree video is a powerful tool that can bring learners into environments that would otherwise be inaccessible. These videos are simultaneously recorded in all directions, allowing the viewer to control viewing direction. Viewers can experience these videos on a computer, smartphone, or tablet or with a virtual reality headset. Camera and software equipment needed to produce 360-degree videos is affordable, allowing Extension educators to produce their own videos. This article addresses the practical aspects of producing 360-degree-video content that can be shared online or in a classroom setting

Stellar Population Models and Individual Element Abundances I: Sensitivity of Stellar Evolution Models

Integrated light from distant galaxies is often compared to stellar population models via the equivalent widths of spectral features--spectral indices--whose strengths rely on the abundances of one or more elements. Such comparisons hinge not only on the overall metal abundance but also on relative abundances. Studies have examined the influence of individual elements on synthetic spectra but little has been done to address similar issues in the stellar evolution models that underlie most stellar population models. Stellar evolution models will primarily be influenced by changes in opacities. In order to explore this issue in detail, twelve sets of stellar evolution tracks and isochrones have been created at constant heavy element mass fraction Z that self-consistently account for varying heavy element mixtures. These sets include scaled-solar, alpha-enhanced, and individual cases where the elements C, N, O, Ne, Mg, Si, S, Ca, Ti, and Fe have been enhanced above their scaled-solar values. The variations that arise between scaled-solar and the other cases are examined with respect to the H-R diagram and main sequence lifetimes.Comment: 33 pages, 13 figures, accepted to Ap

Online Divorce Education: Learning from Participants Who Want More

Divorce education is designed for divorcing parents with minor children, and in a growing number of states, participation has become court-mandated to finalize a divorce. To increase accessibility, some states have adopted brief, online formats for divorce education programming. Evaluations are encouraging; however, less is known about how opinions on course length relate to participants’ views on the benefits of their participation. This study analyzed qualitative data from parents (n = 41) who thought their mandated divorce education course was too short. Results indicate that participants thought the course increased their knowledge of divorce-related matters. They also suggested improvements related to course content priorities. These findings may inform Extension specialists, family life practitioners, and program planners working to improve the delivery of online divorce education programs

Exploiting Connections for Viral Replication.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the COVID-19 (coronavirus disease 2019) pandemic, is a positive strand RNA (+RNA) virus. Like other +RNA viruses, SARS-CoV-2 is dependent on host cell metabolic machinery to survive and replicate, remodeling cellular membranes to generate sites of viral replication. Viral RNA-containing double-membrane vesicles (DMVs) are a striking feature of +RNA viral replication and are abundant in SARS-CoV-2-infected cells. Their generation involves rewiring of host lipid metabolism, including lipid biosynthetic pathways. Viruses can also redirect lipids from host cell organelles; lipid exchange at membrane contact sites, where the membranes of adjacent organelles are in close apposition, has been implicated in the replication of several +RNA viruses. Here we review current understanding of DMV biogenesis. With a focus on the exploitation of contact site machinery by +RNA viruses to generate replication organelles, we discuss evidence that similar mechanisms support SARS-CoV-2 replication, protecting its RNA from the host cell immune response